Isolation and some properties of neosaxitoxin from a xanthid crab Zosimus aeneus
Attempts were made to isolate neosaxitoxin (neoSTX) from a xanthid crab Zosimus aeneus collected at Ishigaki Island, Okinawa Prefecture. Crab specimens were minced and extracted with acidic ethanol, and the extract chromatographed successively on Amberlite IRC-50, Bio-Gel P-2 and Bio-Rex 70 to isola...
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| Veröffentlicht in: | NIPPON SUISAN GAKKAISHI 1985/02/25, Vol.51(2), pp.309-313 |
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| container_title | NIPPON SUISAN GAKKAISHI |
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| creator | Daigo, K Uzu, A Arakawa, O Noguchi, T Hashimoto, K. (Tokyo Univ. (Japan). Faculty of Agriculture) Seto, H |
| description | Attempts were made to isolate neosaxitoxin (neoSTX) from a xanthid crab Zosimus aeneus collected at Ishigaki Island, Okinawa Prefecture. Crab specimens were minced and extracted with acidic ethanol, and the extract chromatographed successively on Amberlite IRC-50, Bio-Gel P-2 and Bio-Rex 70 to isolate neoSTX. The neoSTX preparation thus obtained was homogeneous in both thin-layer chromatography and cellulose acetate membrane electrophoresis. The neoSTX preparation showed a1H-NMR spectrum which coincided well with that in literature. The specific toxicity of this toxin was found to be 3, 900MU/mg. The dose-death time curve was clearly distinguishable from the curve of saxitoxin. Acid hydrolysis resulted in formation of a product whose electrophoretic and thin-layer chromatographic behaviors differed from any of known paralytic shellfish poisons. |
| doi_str_mv | 10.2331/suisan.51.309 |
| format | Article |
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(Tokyo Univ. (Japan). Faculty of Agriculture) ; Seto, H</creator><creatorcontrib>Daigo, K ; Uzu, A ; Arakawa, O ; Noguchi, T ; Hashimoto, K. (Tokyo Univ. (Japan). Faculty of Agriculture) ; Seto, H</creatorcontrib><description>Attempts were made to isolate neosaxitoxin (neoSTX) from a xanthid crab Zosimus aeneus collected at Ishigaki Island, Okinawa Prefecture. Crab specimens were minced and extracted with acidic ethanol, and the extract chromatographed successively on Amberlite IRC-50, Bio-Gel P-2 and Bio-Rex 70 to isolate neoSTX. The neoSTX preparation thus obtained was homogeneous in both thin-layer chromatography and cellulose acetate membrane electrophoresis. The neoSTX preparation showed a1H-NMR spectrum which coincided well with that in literature. The specific toxicity of this toxin was found to be 3, 900MU/mg. The dose-death time curve was clearly distinguishable from the curve of saxitoxin. Acid hydrolysis resulted in formation of a product whose electrophoretic and thin-layer chromatographic behaviors differed from any of known paralytic shellfish poisons.</description><identifier>ISSN: 0021-5392</identifier><identifier>EISSN: 1349-998X</identifier><identifier>DOI: 10.2331/suisan.51.309</identifier><identifier>CODEN: NSUGAF</identifier><language>eng</language><publisher>Tokyo: The Japanese Society of Fisheries Science</publisher><subject>AISLAMIENTO (TECNICA) ; Biological and medical sciences ; CANGREJO DE MAR ; CHEMICOPHYSICAL PROPERTIES ; CRABE ; CRABS ; Crustacea ; Fundamental and applied biological sciences. Psychology ; Invertebrates ; ISOLATION ; ISOLEMENT ; Marine ; NERVOUS SYSTEM DISEASES ; Physiology. Development ; PROPIEDADES FISICO-QUIMICAS ; PROPRIETE PHYSICO-CHIMIQUE ; TOXINAS ; TOXINE ; TOXINS ; TRASTORNOS DEL SISTEMA NERVIOSO ; TROUBLE DU SYSTEME NERVEUX ; Zosimus aeneus</subject><ispartof>NIPPON SUISAN GAKKAISHI, 1985/02/25, Vol.51(2), pp.309-313</ispartof><rights>The Japanese Society of Fisheries Science</rights><rights>1985 INIST-CNRS</rights><rights>Copyright Japan Science and Technology Agency 1985</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c446t-84042cbca89046572605ba7a16120884920fd7ce0678797351b92c2be8e91cdd3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,1876,27903,27904</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=9253575$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Daigo, K</creatorcontrib><creatorcontrib>Uzu, A</creatorcontrib><creatorcontrib>Arakawa, O</creatorcontrib><creatorcontrib>Noguchi, T</creatorcontrib><creatorcontrib>Hashimoto, K. (Tokyo Univ. (Japan). Faculty of Agriculture)</creatorcontrib><creatorcontrib>Seto, H</creatorcontrib><title>Isolation and some properties of neosaxitoxin from a xanthid crab Zosimus aeneus</title><title>NIPPON SUISAN GAKKAISHI</title><addtitle>NSUGAF</addtitle><description>Attempts were made to isolate neosaxitoxin (neoSTX) from a xanthid crab Zosimus aeneus collected at Ishigaki Island, Okinawa Prefecture. Crab specimens were minced and extracted with acidic ethanol, and the extract chromatographed successively on Amberlite IRC-50, Bio-Gel P-2 and Bio-Rex 70 to isolate neoSTX. The neoSTX preparation thus obtained was homogeneous in both thin-layer chromatography and cellulose acetate membrane electrophoresis. The neoSTX preparation showed a1H-NMR spectrum which coincided well with that in literature. The specific toxicity of this toxin was found to be 3, 900MU/mg. The dose-death time curve was clearly distinguishable from the curve of saxitoxin. Acid hydrolysis resulted in formation of a product whose electrophoretic and thin-layer chromatographic behaviors differed from any of known paralytic shellfish poisons.</description><subject>AISLAMIENTO (TECNICA)</subject><subject>Biological and medical sciences</subject><subject>CANGREJO DE MAR</subject><subject>CHEMICOPHYSICAL PROPERTIES</subject><subject>CRABE</subject><subject>CRABS</subject><subject>Crustacea</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Invertebrates</subject><subject>ISOLATION</subject><subject>ISOLEMENT</subject><subject>Marine</subject><subject>NERVOUS SYSTEM DISEASES</subject><subject>Physiology. Development</subject><subject>PROPIEDADES FISICO-QUIMICAS</subject><subject>PROPRIETE PHYSICO-CHIMIQUE</subject><subject>TOXINAS</subject><subject>TOXINE</subject><subject>TOXINS</subject><subject>TRASTORNOS DEL SISTEMA NERVIOSO</subject><subject>TROUBLE DU SYSTEME NERVEUX</subject><subject>Zosimus aeneus</subject><issn>0021-5392</issn><issn>1349-998X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1985</creationdate><recordtype>article</recordtype><recordid>eNpdkE1r3DAQhk1pods0x156ErT05q0-belYQtKkBJpDAyEXMZblRIstbTU2bP99FbzsIRfNQc888_JW1SdGt1wI9h2XgBC3im0FNW-qDRPS1Mboh7fVhlLOaiUMf199QNxRKlqhxaa6u8E0whxSJBB7gmnyZJ_T3uc5eCRpINEnhEOY0yFEMuQ0ESAHiPNz6InL0JHHhGFakICPfsGP1bsBRvTnx3lW3V9d_rm4rm9__7y5-HFbOymbudaSSu46B9pQ2aiWN1R10AJrGKdaS8Pp0LfO06bVrWmFYp3hjndee8Nc34uz6tvqLWn_Lh5nOwV0fhyhBF7QMska2QhawC-vwF1acizZCtNKQwvDC1WvlMsJMfvB7nOYIP-zjNqXdu3arlXMlnYL__VoBXQwDhmiC3haMlwJ1aqCXa3YDmd48qd_KPW60R-lzAj-Ij4-xX8C3DNk62MRfV5FAyQLT7nc-nWnFVWMN-I_SvKbqA</recordid><startdate>19850101</startdate><enddate>19850101</enddate><creator>Daigo, K</creator><creator>Uzu, A</creator><creator>Arakawa, O</creator><creator>Noguchi, T</creator><creator>Hashimoto, K. 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Faculty of Agriculture) ; Seto, H</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c446t-84042cbca89046572605ba7a16120884920fd7ce0678797351b92c2be8e91cdd3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1985</creationdate><topic>AISLAMIENTO (TECNICA)</topic><topic>Biological and medical sciences</topic><topic>CANGREJO DE MAR</topic><topic>CHEMICOPHYSICAL PROPERTIES</topic><topic>CRABE</topic><topic>CRABS</topic><topic>Crustacea</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Invertebrates</topic><topic>ISOLATION</topic><topic>ISOLEMENT</topic><topic>Marine</topic><topic>NERVOUS SYSTEM DISEASES</topic><topic>Physiology. 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Faculty of Agriculture)</creatorcontrib><creatorcontrib>Seto, H</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>Environment Abstracts</collection><collection>Oceanic Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Environment Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 3: Aquatic Pollution & Environmental Quality</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><jtitle>NIPPON SUISAN GAKKAISHI</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Daigo, K</au><au>Uzu, A</au><au>Arakawa, O</au><au>Noguchi, T</au><au>Hashimoto, K. (Tokyo Univ. (Japan). Faculty of Agriculture)</au><au>Seto, H</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Isolation and some properties of neosaxitoxin from a xanthid crab Zosimus aeneus</atitle><jtitle>NIPPON SUISAN GAKKAISHI</jtitle><addtitle>NSUGAF</addtitle><date>1985-01-01</date><risdate>1985</risdate><volume>51</volume><issue>2</issue><spage>309</spage><epage>313</epage><pages>309-313</pages><issn>0021-5392</issn><eissn>1349-998X</eissn><coden>NSUGAF</coden><abstract>Attempts were made to isolate neosaxitoxin (neoSTX) from a xanthid crab Zosimus aeneus collected at Ishigaki Island, Okinawa Prefecture. Crab specimens were minced and extracted with acidic ethanol, and the extract chromatographed successively on Amberlite IRC-50, Bio-Gel P-2 and Bio-Rex 70 to isolate neoSTX. The neoSTX preparation thus obtained was homogeneous in both thin-layer chromatography and cellulose acetate membrane electrophoresis. The neoSTX preparation showed a1H-NMR spectrum which coincided well with that in literature. The specific toxicity of this toxin was found to be 3, 900MU/mg. The dose-death time curve was clearly distinguishable from the curve of saxitoxin. Acid hydrolysis resulted in formation of a product whose electrophoretic and thin-layer chromatographic behaviors differed from any of known paralytic shellfish poisons.</abstract><cop>Tokyo</cop><pub>The Japanese Society of Fisheries Science</pub><doi>10.2331/suisan.51.309</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0021-5392 |
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| source | Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; J-STAGE (Japan Science & Technology Information Aggregator, Electronic) Freely Available Titles - Japanese; AgriKnowledge（アグリナレッジ）AGROLib |
| subjects | AISLAMIENTO (TECNICA) Biological and medical sciences CANGREJO DE MAR CHEMICOPHYSICAL PROPERTIES CRABE CRABS Crustacea Fundamental and applied biological sciences. Psychology Invertebrates ISOLATION ISOLEMENT Marine NERVOUS SYSTEM DISEASES Physiology. Development PROPIEDADES FISICO-QUIMICAS PROPRIETE PHYSICO-CHIMIQUE TOXINAS TOXINE TOXINS TRASTORNOS DEL SISTEMA NERVIOSO TROUBLE DU SYSTEME NERVEUX Zosimus aeneus |
| title | Isolation and some properties of neosaxitoxin from a xanthid crab Zosimus aeneus |
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