Evaluation of Triazole-Chelated Lanthanides as Chemically Stabile Bioimaging Agents
Europium (Eu), dysprosium (Dy), samarium (Sm), and terbium (Tb) complexes were prepared using the neutral tridentate chelator 2,6-bis(1-benzyl-1,2,3-triazol-4-yl)pyridine and one equivalent of each lanthanide salt. The physicochemical, aerodynamic, and in vitro cellular properties of each lanthanide...
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| Veröffentlicht in: | Journal of pharmaceutical sciences 2013-08, Vol.102 (8), p.2589-2598 |
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| creator | Indapurkar, Amruta Henriksen, Brian Tolman, Justin Fletcher, James |
| description | Europium (Eu), dysprosium (Dy), samarium (Sm), and terbium (Tb) complexes were prepared using the neutral tridentate chelator 2,6-bis(1-benzyl-1,2,3-triazol-4-yl)pyridine and one equivalent of each lanthanide salt. The physicochemical, aerodynamic, and in vitro cellular properties of each lanthanide metal complex were studied to determine their viability as cell surface fluorescent probes. Each compound was characterized by electrospray ionization mass spectroscopy (ESI-MS), ultraperformance liquid chromatography (UPLC), differential scanning calorimetry (DSC), and thermogravimetic analysis (TGA). Upon excitation at 320nm each complex displayed characteristic lanthanide-based fluorescence emission in the visible wavelength range with stokes shifts greater than 200nm. Each complex was found to be chemically stable when exposed to pH range of 1–11 for 72h and resistant to photobleaching. To simulate pulmonary administration of these fluorophores, the aerodynamic properties of the Eu and Tb complexes were determined using a next generation impactor (NGI). This measurement confirmed that the complexes retain their fluorescence emission properties after nebulization. Cellular cytotoxicity was determined on A-549 lung cancer cell line using methylthiazol tetrazolium (MTT) cytotoxicity assay at 24, 48, and 72h postexposure to the complexes. The complexes showed a dose and time-dependent effect on the percent viability of the cells. © 2013 Wiley Periodicals, Inc. and the American Pharmacists Association J Pharm Sci 102:2589-2598, 2013 |
| doi_str_mv | 10.1002/jps.23616 |
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The physicochemical, aerodynamic, and in vitro cellular properties of each lanthanide metal complex were studied to determine their viability as cell surface fluorescent probes. Each compound was characterized by electrospray ionization mass spectroscopy (ESI-MS), ultraperformance liquid chromatography (UPLC), differential scanning calorimetry (DSC), and thermogravimetic analysis (TGA). Upon excitation at 320nm each complex displayed characteristic lanthanide-based fluorescence emission in the visible wavelength range with stokes shifts greater than 200nm. Each complex was found to be chemically stable when exposed to pH range of 1–11 for 72h and resistant to photobleaching. To simulate pulmonary administration of these fluorophores, the aerodynamic properties of the Eu and Tb complexes were determined using a next generation impactor (NGI). This measurement confirmed that the complexes retain their fluorescence emission properties after nebulization. Cellular cytotoxicity was determined on A-549 lung cancer cell line using methylthiazol tetrazolium (MTT) cytotoxicity assay at 24, 48, and 72h postexposure to the complexes. 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The physicochemical, aerodynamic, and in vitro cellular properties of each lanthanide metal complex were studied to determine their viability as cell surface fluorescent probes. Each compound was characterized by electrospray ionization mass spectroscopy (ESI-MS), ultraperformance liquid chromatography (UPLC), differential scanning calorimetry (DSC), and thermogravimetic analysis (TGA). Upon excitation at 320nm each complex displayed characteristic lanthanide-based fluorescence emission in the visible wavelength range with stokes shifts greater than 200nm. Each complex was found to be chemically stable when exposed to pH range of 1–11 for 72h and resistant to photobleaching. To simulate pulmonary administration of these fluorophores, the aerodynamic properties of the Eu and Tb complexes were determined using a next generation impactor (NGI). This measurement confirmed that the complexes retain their fluorescence emission properties after nebulization. Cellular cytotoxicity was determined on A-549 lung cancer cell line using methylthiazol tetrazolium (MTT) cytotoxicity assay at 24, 48, and 72h postexposure to the complexes. The complexes showed a dose and time-dependent effect on the percent viability of the cells. © 2013 Wiley Periodicals, Inc. and the American Pharmacists Association J Pharm Sci 102:2589-2598, 2013</description><subject>Aerodynamic properties</subject><subject>Calorimetry</subject><subject>cell culture</subject><subject>Cell Line, Tumor</subject><subject>Cell surface</subject><subject>Cell Survival - drug effects</subject><subject>Chelating Agents - chemistry</subject><subject>Chelating Agents - toxicity</subject><subject>chelation</subject><subject>Chemical compounds</subject><subject>Coordination Complexes - chemistry</subject><subject>Coordination Complexes - toxicity</subject><subject>Coordination compounds</subject><subject>Cytotoxicity</subject><subject>Differential scanning calorimetry</subject><subject>Dysprosium</subject><subject>Emissions</subject><subject>Europium</subject><subject>Fluorescence</subject><subject>fluorescence spectroscopy</subject><subject>Fluorescent Dyes - chemistry</subject><subject>Fluorescent Dyes - toxicity</subject><subject>Fluorescent indicators</subject><subject>fluorescent probes</subject><subject>Fluorophores</subject><subject>Humans</subject><subject>Ionization</subject><subject>Lanthanides</subject><subject>Lanthanoid Series Elements - chemistry</subject><subject>Lanthanoid Series Elements - toxicity</subject><subject>Liquid chromatography</subject><subject>Lung cancer</subject><subject>Mass spectroscopy</subject><subject>Medical imaging</subject><subject>Photobleaching</subject><subject>Samarium</subject><subject>Solubility</subject><subject>Terbium</subject><subject>thermal analysis</subject><subject>Thermogravimetric analysis</subject><subject>Toxicity</subject><subject>Triazoles - chemistry</subject><subject>Triazoles - toxicity</subject><issn>0022-3549</issn><issn>1520-6017</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kc1uEzEUhS0EoqGw4AXQSGzKYlr_z3hZopYfRSpSsrduPXdSR8442DNF6dPXJS0LBF1Zsj9_uvccQt4zesoo5WebXT7lQjP9gsyY4rTWlDUvyay88VooaY7Im5w3lFJNlXpNjrhoNKPMzMjy4hbCBKOPQxX7apU83MWA9fwGA4zYVQsYxhsYfIe5glyV-613EMK-Wo5w7QNWn330W1j7YV2dr3EY81vyqoeQ8d3jeUxWlxer-dd6cfXl2_x8UTvJla7ROZTC0LZtNRegm8ZobDiT2EqqTKeQK8fBKaV63UDbQ4eKA1PQQW-MOCYnB-0uxZ8T5tFufXYYAgwYp2yZZJpK3oqmoB__QjdxSkMZzvISG-eNZOY5qrikEkybB9enA-VSzDlhb3ep7J_2llH7UIctddjfdRT2w6Nxut5i94d8yr8AZwfgV0ly_3-T_f5j-aQUhx9Ykr31mGx2HgeHnU_oRttF_49B7gH-VKOG</recordid><startdate>201308</startdate><enddate>201308</enddate><creator>Indapurkar, Amruta</creator><creator>Henriksen, Brian</creator><creator>Tolman, Justin</creator><creator>Fletcher, James</creator><general>Elsevier Inc</general><general>Wiley Subscription Services, Inc., A Wiley Company</general><general>Elsevier Limited</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7QP</scope><scope>7QR</scope><scope>7TK</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>201308</creationdate><title>Evaluation of Triazole-Chelated Lanthanides as Chemically Stabile Bioimaging Agents</title><author>Indapurkar, Amruta ; 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The physicochemical, aerodynamic, and in vitro cellular properties of each lanthanide metal complex were studied to determine their viability as cell surface fluorescent probes. Each compound was characterized by electrospray ionization mass spectroscopy (ESI-MS), ultraperformance liquid chromatography (UPLC), differential scanning calorimetry (DSC), and thermogravimetic analysis (TGA). Upon excitation at 320nm each complex displayed characteristic lanthanide-based fluorescence emission in the visible wavelength range with stokes shifts greater than 200nm. Each complex was found to be chemically stable when exposed to pH range of 1–11 for 72h and resistant to photobleaching. To simulate pulmonary administration of these fluorophores, the aerodynamic properties of the Eu and Tb complexes were determined using a next generation impactor (NGI). This measurement confirmed that the complexes retain their fluorescence emission properties after nebulization. Cellular cytotoxicity was determined on A-549 lung cancer cell line using methylthiazol tetrazolium (MTT) cytotoxicity assay at 24, 48, and 72h postexposure to the complexes. The complexes showed a dose and time-dependent effect on the percent viability of the cells. © 2013 Wiley Periodicals, Inc. and the American Pharmacists Association J Pharm Sci 102:2589-2598, 2013</abstract><cop>Hoboken</cop><pub>Elsevier Inc</pub><pmid>23761019</pmid><doi>10.1002/jps.23616</doi><tpages>10</tpages></addata></record> |
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| subjects | Aerodynamic properties Calorimetry cell culture Cell Line, Tumor Cell surface Cell Survival - drug effects Chelating Agents - chemistry Chelating Agents - toxicity chelation Chemical compounds Coordination Complexes - chemistry Coordination Complexes - toxicity Coordination compounds Cytotoxicity Differential scanning calorimetry Dysprosium Emissions Europium Fluorescence fluorescence spectroscopy Fluorescent Dyes - chemistry Fluorescent Dyes - toxicity Fluorescent indicators fluorescent probes Fluorophores Humans Ionization Lanthanides Lanthanoid Series Elements - chemistry Lanthanoid Series Elements - toxicity Liquid chromatography Lung cancer Mass spectroscopy Medical imaging Photobleaching Samarium Solubility Terbium thermal analysis Thermogravimetric analysis Toxicity Triazoles - chemistry Triazoles - toxicity |
| title | Evaluation of Triazole-Chelated Lanthanides as Chemically Stabile Bioimaging Agents |
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