Protoplast fusion used for the construction of presumptive polyploids of the D-xylose fermenting yeast Candida shehatae
Protoplast fusion technique was employed in the preparation of presumptive diploid, triploid and tetraploid strains of the D-xylose fermenting yeast C. shehatae CBS2779. Prototrophic selection technique was employed for the recovery of presumed fusant strains. The hybrid nature of the presumptive di...
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| Veröffentlicht in: | Current genetics 1985-01, Vol.9 (4), p.313-319 |
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| creator | Johannsen, E Eagle, L Bredenhann, G |
| description | Protoplast fusion technique was employed in the preparation of presumptive diploid, triploid and tetraploid strains of the D-xylose fermenting yeast C. shehatae CBS2779. Prototrophic selection technique was employed for the recovery of presumed fusant strains. The hybrid nature of the presumptive diploid, triploid and tetraploid strains was confirmed by analyzing: I) the nuclear condition; II) the cell size and the cell volume of the parental and fusant strain; III) the cellular DNA content; and IV) the induced and spontanenous mitotic segregation of properties in these strains. The increased level of ploidy was found to have an effect on the rate of ethanol production from D-xylose. |
| doi_str_mv | 10.1007/BF00419961 |
| format | Article |
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Prototrophic selection technique was employed for the recovery of presumed fusant strains. The hybrid nature of the presumptive diploid, triploid and tetraploid strains was confirmed by analyzing: I) the nuclear condition; II) the cell size and the cell volume of the parental and fusant strain; III) the cellular DNA content; and IV) the induced and spontanenous mitotic segregation of properties in these strains. The increased level of ploidy was found to have an effect on the rate of ethanol production from D-xylose.</description><identifier>ISSN: 0172-8083</identifier><identifier>EISSN: 1432-0983</identifier><identifier>DOI: 10.1007/BF00419961</identifier><identifier>CODEN: CUGED5</identifier><language>eng</language><publisher>Heidelberg: Springer</publisher><subject>Applied microbiology ; Biological and medical sciences ; Biotechnology ; Candida ; Candida shehatae ; D-xylose ; ethanol ; Eukaryotes ; fermentation ; Fundamental and applied biological sciences. 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Prototrophic selection technique was employed for the recovery of presumed fusant strains. The hybrid nature of the presumptive diploid, triploid and tetraploid strains was confirmed by analyzing: I) the nuclear condition; II) the cell size and the cell volume of the parental and fusant strain; III) the cellular DNA content; and IV) the induced and spontanenous mitotic segregation of properties in these strains. The increased level of ploidy was found to have an effect on the rate of ethanol production from D-xylose.</description><subject>Applied microbiology</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Candida</subject><subject>Candida shehatae</subject><subject>D-xylose</subject><subject>ethanol</subject><subject>Eukaryotes</subject><subject>fermentation</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genetic technics</subject><subject>Methods. Procedures. Technologies</subject><subject>Microbiology</subject><subject>Miscellaneous</subject><subject>Mutant screening</subject><subject>polyploids</subject><subject>polyploidy</subject><subject>protoplast fusion</subject><subject>strains</subject><subject>xylose</subject><issn>0172-8083</issn><issn>1432-0983</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1985</creationdate><recordtype>article</recordtype><recordid>eNpFkEFP3DAQha2qSN0uXPoH6kPFASnFjh3bOcJSoNJKIMGeI8cZ76bKxsHjAPvvm2hRe5qR3jdP8x4h3zj7yRnTl9e3jElelop_IgsuRZ6x0ojPZMG4zjPDjPhCviL-YYznptQL8vYYQwpDZzFRP2IbejoiNNSHSNMOqAs9pji6NCvB0yECjvshta9Ah9Adhi60Dc7KTN9k74cuIFAPcQ99avstPcDsvbJ90zaW4g52Nlk4JSfedghnH3NJNre_nlf32frh7vfqap05wUXKtFO5ckUNhXMwraap6xK05brwtffOuEJLplytjC4FTCFV0xRS1jXLlVRCLMn50XeI4WUETNW-RQddZ3sII1Zccqlzwybw4gi6GBAj-GqI7d7GQ8VZNXdb_e92gn98uFp0tvPR9q7FfxdGKiX5jH0_Yt6Gym7jhGyecsbF9Jwo88KIvxdVhBk</recordid><startdate>19850101</startdate><enddate>19850101</enddate><creator>Johannsen, E</creator><creator>Eagle, L</creator><creator>Bredenhann, G</creator><general>Springer</general><scope>FBQ</scope><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7T7</scope><scope>7TM</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>19850101</creationdate><title>Protoplast fusion used for the construction of presumptive polyploids of the D-xylose fermenting yeast Candida shehatae</title><author>Johannsen, E ; Eagle, L ; Bredenhann, G</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c313t-7c626c5be5cce6268dbb9e7a175fbffc8c57406cb68793e1726dd544bb0264633</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1985</creationdate><topic>Applied microbiology</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>Candida</topic><topic>Candida shehatae</topic><topic>D-xylose</topic><topic>ethanol</topic><topic>Eukaryotes</topic><topic>fermentation</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genetic technics</topic><topic>Methods. Procedures. Technologies</topic><topic>Microbiology</topic><topic>Miscellaneous</topic><topic>Mutant screening</topic><topic>polyploids</topic><topic>polyploidy</topic><topic>protoplast fusion</topic><topic>strains</topic><topic>xylose</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Johannsen, E</creatorcontrib><creatorcontrib>Eagle, L</creatorcontrib><creatorcontrib>Bredenhann, G</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Current genetics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Johannsen, E</au><au>Eagle, L</au><au>Bredenhann, G</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Protoplast fusion used for the construction of presumptive polyploids of the D-xylose fermenting yeast Candida shehatae</atitle><jtitle>Current genetics</jtitle><date>1985-01-01</date><risdate>1985</risdate><volume>9</volume><issue>4</issue><spage>313</spage><epage>319</epage><pages>313-319</pages><issn>0172-8083</issn><eissn>1432-0983</eissn><coden>CUGED5</coden><abstract>Protoplast fusion technique was employed in the preparation of presumptive diploid, triploid and tetraploid strains of the D-xylose fermenting yeast C. shehatae CBS2779. Prototrophic selection technique was employed for the recovery of presumed fusant strains. The hybrid nature of the presumptive diploid, triploid and tetraploid strains was confirmed by analyzing: I) the nuclear condition; II) the cell size and the cell volume of the parental and fusant strain; III) the cellular DNA content; and IV) the induced and spontanenous mitotic segregation of properties in these strains. The increased level of ploidy was found to have an effect on the rate of ethanol production from D-xylose.</abstract><cop>Heidelberg</cop><cop>Berlin</cop><pub>Springer</pub><doi>10.1007/BF00419961</doi><tpages>7</tpages></addata></record> |
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| identifier | ISSN: 0172-8083 |
| ispartof | Current genetics, 1985-01, Vol.9 (4), p.313-319 |
| issn | 0172-8083 1432-0983 |
| language | eng |
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| source | SpringerNature Journals |
| subjects | Applied microbiology Biological and medical sciences Biotechnology Candida Candida shehatae D-xylose ethanol Eukaryotes fermentation Fundamental and applied biological sciences. Psychology Genetic technics Methods. Procedures. Technologies Microbiology Miscellaneous Mutant screening polyploids polyploidy protoplast fusion strains xylose |
| title | Protoplast fusion used for the construction of presumptive polyploids of the D-xylose fermenting yeast Candida shehatae |
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