Hanganutziu-Deicher Heterophile Antigen in Human Retinoblastoma Cells
We investigated the possible existence of Hanganutziu-Deicher antigen (HD antigen) on retinoblastoma cells by means of indirect membrane immunofluorescence and a complement-dependent antibody-mediated cytotoxicity test. We prepared antiserum in the chicken by immunization with purified equine hemato...
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| Veröffentlicht in: | American journal of ophthalmology 1983-09, Vol.96 (3), p.321-325 |
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| container_title | American journal of ophthalmology |
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| creator | Ohashi, Yuichi Sasabe, Testuo Nishida, Teruo Nishi, Yasuaki Higashi, Hideyoshi |
| description | We investigated the possible existence of Hanganutziu-Deicher antigen (HD antigen) on retinoblastoma cells by means of indirect membrane immunofluorescence and a complement-dependent antibody-mediated cytotoxicity test. We prepared antiserum in the chicken by immunization with purified equine hematoside highly specific to HD antigen. Three kinds of retinoblastoma cell lines, Y-79, WERI-Rb 1, and TOTL-1, showed positive membrane staining. Y-79 and TOTL-1 cells were also confirmed by the cytotoxicity test to possess HD antigen. Retinoblastoma cells freshly isolated from two patients had a positive membrane immunofluorescence. These results indicated that HD antigen was expressed in human retinoblastoma cells in vitro as well as in vivo. |
| doi_str_mv | 10.1016/S0002-9394(14)77822-5 |
| format | Article |
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We prepared antiserum in the chicken by immunization with purified equine hematoside highly specific to HD antigen. Three kinds of retinoblastoma cell lines, Y-79, WERI-Rb 1, and TOTL-1, showed positive membrane staining. Y-79 and TOTL-1 cells were also confirmed by the cytotoxicity test to possess HD antigen. Retinoblastoma cells freshly isolated from two patients had a positive membrane immunofluorescence. 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We prepared antiserum in the chicken by immunization with purified equine hematoside highly specific to HD antigen. Three kinds of retinoblastoma cell lines, Y-79, WERI-Rb 1, and TOTL-1, showed positive membrane staining. Y-79 and TOTL-1 cells were also confirmed by the cytotoxicity test to possess HD antigen. Retinoblastoma cells freshly isolated from two patients had a positive membrane immunofluorescence. These results indicated that HD antigen was expressed in human retinoblastoma cells in vitro as well as in vivo.</description><subject>antigens</subject><subject>Antigens, Heterophile - analysis</subject><subject>Antigens, Neoplasm - analysis</subject><subject>Antigens, Surface - analysis</subject><subject>Cell Line</subject><subject>cell surface</subject><subject>Cytotoxicity Tests, Immunologic</subject><subject>Eye Neoplasms - immunology</subject><subject>Fluorescent Antibody Technique</subject><subject>Humans</subject><subject>man</subject><subject>retina</subject><subject>Retinoblastoma - immunology</subject><subject>tumor cells</subject><issn>0002-9394</issn><issn>1879-1891</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1983</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkMlOwzAQhi0EKmV5hEo5ITgEbGdzTqgqhSBVQmI5W44zbo0Sp9gOEjw97qJeucxoNP8_y4fQhOBbgkl-94YxpnGZlOk1SW-KglEaZ0doTFhRxoSV5BiND5JTdObcZyjzIi1GaJQnGckpHaN5JcxSmMH_6iF-AC1XYKMKPNh-vdItRFPj9RJMpE1UDZ0w0St4bfq6Fc73nYhm0LbuAp0o0Tq43Odz9PE4f59V8eLl6Xk2XcQyoYmPCZBcylQwVsqcFoAhBAW0KZq6TgnFVClgShQ1YQrLVGGRM5BKqYQEV5mco6vd3LXtvwZwnnfayXCBMNAPjpMUl6zM0iDMdkJpe-csKL62uhP2hxPMN_j4Fh_fsAkuvsXHs-Cb7BcMdQfNwbXnFfr3uz6EL781WO6kBiOh0Rak502v_9nwB0J9gCE</recordid><startdate>198309</startdate><enddate>198309</enddate><creator>Ohashi, Yuichi</creator><creator>Sasabe, Testuo</creator><creator>Nishida, Teruo</creator><creator>Nishi, Yasuaki</creator><creator>Higashi, Hideyoshi</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>M7Z</scope><scope>P64</scope></search><sort><creationdate>198309</creationdate><title>Hanganutziu-Deicher Heterophile Antigen in Human Retinoblastoma Cells</title><author>Ohashi, Yuichi ; Sasabe, Testuo ; Nishida, Teruo ; Nishi, Yasuaki ; Higashi, Hideyoshi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c323t-1e16cc4a889c627e0e27efe2d7dbb41202ffe8fa7b18f0c4f0a68ecfff314a893</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1983</creationdate><topic>antigens</topic><topic>Antigens, Heterophile - analysis</topic><topic>Antigens, Neoplasm - analysis</topic><topic>Antigens, Surface - analysis</topic><topic>Cell Line</topic><topic>cell surface</topic><topic>Cytotoxicity Tests, Immunologic</topic><topic>Eye Neoplasms - immunology</topic><topic>Fluorescent Antibody Technique</topic><topic>Humans</topic><topic>man</topic><topic>retina</topic><topic>Retinoblastoma - immunology</topic><topic>tumor cells</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ohashi, Yuichi</creatorcontrib><creatorcontrib>Sasabe, Testuo</creatorcontrib><creatorcontrib>Nishida, Teruo</creatorcontrib><creatorcontrib>Nishi, Yasuaki</creatorcontrib><creatorcontrib>Higashi, Hideyoshi</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biochemistry Abstracts 1</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>American journal of ophthalmology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ohashi, Yuichi</au><au>Sasabe, Testuo</au><au>Nishida, Teruo</au><au>Nishi, Yasuaki</au><au>Higashi, Hideyoshi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Hanganutziu-Deicher Heterophile Antigen in Human Retinoblastoma Cells</atitle><jtitle>American journal of ophthalmology</jtitle><addtitle>Am J Ophthalmol</addtitle><date>1983-09</date><risdate>1983</risdate><volume>96</volume><issue>3</issue><spage>321</spage><epage>325</epage><pages>321-325</pages><issn>0002-9394</issn><eissn>1879-1891</eissn><abstract>We investigated the possible existence of Hanganutziu-Deicher antigen (HD antigen) on retinoblastoma cells by means of indirect membrane immunofluorescence and a complement-dependent antibody-mediated cytotoxicity test. We prepared antiserum in the chicken by immunization with purified equine hematoside highly specific to HD antigen. Three kinds of retinoblastoma cell lines, Y-79, WERI-Rb 1, and TOTL-1, showed positive membrane staining. Y-79 and TOTL-1 cells were also confirmed by the cytotoxicity test to possess HD antigen. Retinoblastoma cells freshly isolated from two patients had a positive membrane immunofluorescence. These results indicated that HD antigen was expressed in human retinoblastoma cells in vitro as well as in vivo.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>6351622</pmid><doi>10.1016/S0002-9394(14)77822-5</doi><tpages>5</tpages></addata></record> |
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| ispartof | American journal of ophthalmology, 1983-09, Vol.96 (3), p.321-325 |
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| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | antigens Antigens, Heterophile - analysis Antigens, Neoplasm - analysis Antigens, Surface - analysis Cell Line cell surface Cytotoxicity Tests, Immunologic Eye Neoplasms - immunology Fluorescent Antibody Technique Humans man retina Retinoblastoma - immunology tumor cells |
| title | Hanganutziu-Deicher Heterophile Antigen in Human Retinoblastoma Cells |
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