Hanganutziu-Deicher Heterophile Antigen in Human Retinoblastoma Cells

We investigated the possible existence of Hanganutziu-Deicher antigen (HD antigen) on retinoblastoma cells by means of indirect membrane immunofluorescence and a complement-dependent antibody-mediated cytotoxicity test. We prepared antiserum in the chicken by immunization with purified equine hemato...

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Veröffentlicht in:	American journal of ophthalmology 1983-09, Vol.96 (3), p.321-325
Hauptverfasser:	Ohashi, Yuichi, Sasabe, Testuo, Nishida, Teruo, Nishi, Yasuaki, Higashi, Hideyoshi
Format:	Artikel
Sprache:	eng
Schlagworte:	antigens Antigens, Heterophile - analysis Antigens, Neoplasm - analysis Antigens, Surface - analysis Cell Line cell surface Cytotoxicity Tests, Immunologic Eye Neoplasms - immunology Fluorescent Antibody Technique Humans man retina Retinoblastoma - immunology tumor cells
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Beschreibung
Zusammenfassung:	We investigated the possible existence of Hanganutziu-Deicher antigen (HD antigen) on retinoblastoma cells by means of indirect membrane immunofluorescence and a complement-dependent antibody-mediated cytotoxicity test. We prepared antiserum in the chicken by immunization with purified equine hematoside highly specific to HD antigen. Three kinds of retinoblastoma cell lines, Y-79, WERI-Rb 1, and TOTL-1, showed positive membrane staining. Y-79 and TOTL-1 cells were also confirmed by the cytotoxicity test to possess HD antigen. Retinoblastoma cells freshly isolated from two patients had a positive membrane immunofluorescence. These results indicated that HD antigen was expressed in human retinoblastoma cells in vitro as well as in vivo.
ISSN:	0002-9394 1879-1891
DOI:	10.1016/S0002-9394(14)77822-5