Genetic effects of injection of Rous sarcoma virus DNA into polar plasm of early Drosophila melanogaster embryos [Microinjection technique]
Retroviral proviruses and the transposable elements of eukaryotic genomes are structurally similar 1–3 . The biological significance of eukaryotic transposable elements has not been examined extensively but it is known that, like prokaryotic transposons, these elements can induce mutations in adjace...
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Veröffentlicht in: | Nature (London) 1984-01, Vol.311 (5984), p.392-394 |
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creator | Gazaryan, K.G Nabirochkin, S.D Tatosyan, A.G Shakhbazyan, A.K Shibanova, E.N |
description | Retroviral proviruses and the transposable elements of eukaryotic genomes are structurally similar
1–3
. The biological significance of eukaryotic transposable elements has not been examined extensively but it is known that, like prokaryotic transposons, these elements can induce mutations in adjacent genes and cause their transposition
4,5
. It is of interest to determine whether retroviral proviruses have the same mutagenic
6
and gene transposing ability as transposable elements, particularly because the retrovirus genome is assumed to have originated from transposable elements of lower eukaryotes
1
. The transfer of DNA sequences into animal zygotes or embryos by microinjection is a promising experimental approach for eluxidating their functions
7–11
: when foreign DNAs were introduced into a mouse germ line, mutations were induced
9–11
and at least in some mice, the mutation was caused by the insertion of a retroviral sequence
9,10
. We have introduced Rous sarcoma virus (RSV) DNA into a germ line of
Drosophila melanogaster
, and describe here the resultant genetic effects. |
doi_str_mv | 10.1038/311392a0 |
format | Article |
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1–3
. The biological significance of eukaryotic transposable elements has not been examined extensively but it is known that, like prokaryotic transposons, these elements can induce mutations in adjacent genes and cause their transposition
4,5
. It is of interest to determine whether retroviral proviruses have the same mutagenic
6
and gene transposing ability as transposable elements, particularly because the retrovirus genome is assumed to have originated from transposable elements of lower eukaryotes
1
. The transfer of DNA sequences into animal zygotes or embryos by microinjection is a promising experimental approach for eluxidating their functions
7–11
: when foreign DNAs were introduced into a mouse germ line, mutations were induced
9–11
and at least in some mice, the mutation was caused by the insertion of a retroviral sequence
9,10
. We have introduced Rous sarcoma virus (RSV) DNA into a germ line of
Drosophila melanogaster
, and describe here the resultant genetic effects.</description><identifier>ISSN: 0028-0836</identifier><identifier>EISSN: 1476-4687</identifier><identifier>DOI: 10.1038/311392a0</identifier><identifier>PMID: 6090937</identifier><identifier>CODEN: NATUAS</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>Animals ; Avian Sarcoma Viruses - genetics ; Biological and medical sciences ; DNA ; DNA - administration & dosage ; DNA, Viral - administration & dosage ; DNA, Viral - genetics ; Drosophila melanogaster ; Drosophila melanogaster - embryology ; Drosophila melanogaster - genetics ; embryo (animal) ; Embryo, Nonmammalian - physiology ; Female ; Fundamental and applied biological sciences. Psychology ; genetic engineering ; Humanities and Social Sciences ; letter ; Microinjections ; Molecular and cellular biology ; Molecular genetics ; multidisciplinary ; Mutagenesis. Repair ; Mutation ; Phenotype ; Plasmids ; Rous sarcoma virus ; Science ; Science (multidisciplinary)</subject><ispartof>Nature (London), 1984-01, Vol.311 (5984), p.392-394</ispartof><rights>Springer Nature Limited 1984</rights><rights>1985 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c419t-f1b128db3d3c8105f3fc054cb9f8b44bfbaaf0728940004d7c6b6ae1a05ae6d13</citedby><cites>FETCH-LOGICAL-c419t-f1b128db3d3c8105f3fc054cb9f8b44bfbaaf0728940004d7c6b6ae1a05ae6d13</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,2727,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=8951454$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/6090937$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Gazaryan, K.G</creatorcontrib><creatorcontrib>Nabirochkin, S.D</creatorcontrib><creatorcontrib>Tatosyan, A.G</creatorcontrib><creatorcontrib>Shakhbazyan, A.K</creatorcontrib><creatorcontrib>Shibanova, E.N</creatorcontrib><title>Genetic effects of injection of Rous sarcoma virus DNA into polar plasm of early Drosophila melanogaster embryos [Microinjection technique]</title><title>Nature (London)</title><addtitle>Nature</addtitle><addtitle>Nature</addtitle><description>Retroviral proviruses and the transposable elements of eukaryotic genomes are structurally similar
1–3
. The biological significance of eukaryotic transposable elements has not been examined extensively but it is known that, like prokaryotic transposons, these elements can induce mutations in adjacent genes and cause their transposition
4,5
. It is of interest to determine whether retroviral proviruses have the same mutagenic
6
and gene transposing ability as transposable elements, particularly because the retrovirus genome is assumed to have originated from transposable elements of lower eukaryotes
1
. The transfer of DNA sequences into animal zygotes or embryos by microinjection is a promising experimental approach for eluxidating their functions
7–11
: when foreign DNAs were introduced into a mouse germ line, mutations were induced
9–11
and at least in some mice, the mutation was caused by the insertion of a retroviral sequence
9,10
. We have introduced Rous sarcoma virus (RSV) DNA into a germ line of
Drosophila melanogaster
, and describe here the resultant genetic effects.</description><subject>Animals</subject><subject>Avian Sarcoma Viruses - genetics</subject><subject>Biological and medical sciences</subject><subject>DNA</subject><subject>DNA - administration & dosage</subject><subject>DNA, Viral - administration & dosage</subject><subject>DNA, Viral - genetics</subject><subject>Drosophila melanogaster</subject><subject>Drosophila melanogaster - embryology</subject><subject>Drosophila melanogaster - genetics</subject><subject>embryo (animal)</subject><subject>Embryo, Nonmammalian - physiology</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>genetic engineering</subject><subject>Humanities and Social Sciences</subject><subject>letter</subject><subject>Microinjections</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>multidisciplinary</subject><subject>Mutagenesis. Repair</subject><subject>Mutation</subject><subject>Phenotype</subject><subject>Plasmids</subject><subject>Rous sarcoma virus</subject><subject>Science</subject><subject>Science (multidisciplinary)</subject><issn>0028-0836</issn><issn>1476-4687</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1984</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNptkEFv1DAQhS0EKktB4g8APiAEhxQ7dhznWLVQkEorFXpCKJo4461XSZzaCdL-Bv40XmVZOHCyR-_TezOPkOecnXAm9HvBuahyYA_IistSZVLp8iFZMZbrjGmhHpMnMW4YYwUv5RE5UqxilShX5NcFDjg5Q9FaNFOk3lI3bNLX-WE33Pg50gjB-B7oTxfSdH51mpjJ09F3EOjYQex3KELotvQ8-OjHO9cB7bGDwa8hThgo9k3Y-ki_f3Em-L8ZE5q7wd3P-OMpeWShi_hs_x6T248fvp19yi6vLz6fnV5mRvJqyixveK7bRrTCaM4KK6xhhTRNZXUjZWMbAMvKXFcyHSzb0qhGAXJgBaBquTgmbxbfMfiUG6e6d9Fgl5bFdG3NJVNloVUC3y5gWjjGgLYeg-shbGvO6l3v9Z_eE_pi7zk3PbYHcF900l_vdYgGOhtgMC4eMF0VXBYyYe8WLCZlWGOoN34OQ2rjf5EvF3aAaQ548PoHeLUAFnwN65Dybr_mjAvGpap0wn4DvGKtCw</recordid><startdate>19840101</startdate><enddate>19840101</enddate><creator>Gazaryan, K.G</creator><creator>Nabirochkin, S.D</creator><creator>Tatosyan, A.G</creator><creator>Shakhbazyan, A.K</creator><creator>Shibanova, E.N</creator><general>Nature Publishing Group UK</general><general>Nature Publishing</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>7U9</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>19840101</creationdate><title>Genetic effects of injection of Rous sarcoma virus DNA into polar plasm of early Drosophila melanogaster embryos [Microinjection technique]</title><author>Gazaryan, K.G ; Nabirochkin, S.D ; Tatosyan, A.G ; Shakhbazyan, A.K ; Shibanova, E.N</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c419t-f1b128db3d3c8105f3fc054cb9f8b44bfbaaf0728940004d7c6b6ae1a05ae6d13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1984</creationdate><topic>Animals</topic><topic>Avian Sarcoma Viruses - genetics</topic><topic>Biological and medical sciences</topic><topic>DNA</topic><topic>DNA - administration & dosage</topic><topic>DNA, Viral - administration & dosage</topic><topic>DNA, Viral - genetics</topic><topic>Drosophila melanogaster</topic><topic>Drosophila melanogaster - embryology</topic><topic>Drosophila melanogaster - genetics</topic><topic>embryo (animal)</topic><topic>Embryo, Nonmammalian - physiology</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>genetic engineering</topic><topic>Humanities and Social Sciences</topic><topic>letter</topic><topic>Microinjections</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>multidisciplinary</topic><topic>Mutagenesis. Repair</topic><topic>Mutation</topic><topic>Phenotype</topic><topic>Plasmids</topic><topic>Rous sarcoma virus</topic><topic>Science</topic><topic>Science (multidisciplinary)</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gazaryan, K.G</creatorcontrib><creatorcontrib>Nabirochkin, S.D</creatorcontrib><creatorcontrib>Tatosyan, A.G</creatorcontrib><creatorcontrib>Shakhbazyan, A.K</creatorcontrib><creatorcontrib>Shibanova, E.N</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Nature (London)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gazaryan, K.G</au><au>Nabirochkin, S.D</au><au>Tatosyan, A.G</au><au>Shakhbazyan, A.K</au><au>Shibanova, E.N</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Genetic effects of injection of Rous sarcoma virus DNA into polar plasm of early Drosophila melanogaster embryos [Microinjection technique]</atitle><jtitle>Nature (London)</jtitle><stitle>Nature</stitle><addtitle>Nature</addtitle><date>1984-01-01</date><risdate>1984</risdate><volume>311</volume><issue>5984</issue><spage>392</spage><epage>394</epage><pages>392-394</pages><issn>0028-0836</issn><eissn>1476-4687</eissn><coden>NATUAS</coden><abstract>Retroviral proviruses and the transposable elements of eukaryotic genomes are structurally similar
1–3
. The biological significance of eukaryotic transposable elements has not been examined extensively but it is known that, like prokaryotic transposons, these elements can induce mutations in adjacent genes and cause their transposition
4,5
. It is of interest to determine whether retroviral proviruses have the same mutagenic
6
and gene transposing ability as transposable elements, particularly because the retrovirus genome is assumed to have originated from transposable elements of lower eukaryotes
1
. The transfer of DNA sequences into animal zygotes or embryos by microinjection is a promising experimental approach for eluxidating their functions
7–11
: when foreign DNAs were introduced into a mouse germ line, mutations were induced
9–11
and at least in some mice, the mutation was caused by the insertion of a retroviral sequence
9,10
. We have introduced Rous sarcoma virus (RSV) DNA into a germ line of
Drosophila melanogaster
, and describe here the resultant genetic effects.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>6090937</pmid><doi>10.1038/311392a0</doi><tpages>3</tpages></addata></record> |
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subjects | Animals Avian Sarcoma Viruses - genetics Biological and medical sciences DNA DNA - administration & dosage DNA, Viral - administration & dosage DNA, Viral - genetics Drosophila melanogaster Drosophila melanogaster - embryology Drosophila melanogaster - genetics embryo (animal) Embryo, Nonmammalian - physiology Female Fundamental and applied biological sciences. Psychology genetic engineering Humanities and Social Sciences letter Microinjections Molecular and cellular biology Molecular genetics multidisciplinary Mutagenesis. Repair Mutation Phenotype Plasmids Rous sarcoma virus Science Science (multidisciplinary) |
title | Genetic effects of injection of Rous sarcoma virus DNA into polar plasm of early Drosophila melanogaster embryos [Microinjection technique] |
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