Esophageal microsomal metabolism of N-nitrosomethylbenzylamine in the zinc-deficient rat

Epidemiological studies in China suggest that dietary zinc deficiency and environmental exposure to N-nitrosamine carcinogens, such as N-nitrosomethylbenzylamine (NMBA), are among the factors associated with an increased incidence of esophageal carcinoma in humans. NMBA belongs to a class of nitrosa...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Cancer research (Chicago, Ill.) Ill.), 1984-12, Vol.44 (12), p.5629-5633
Hauptverfasser: BARCH, D. H, KUEMMERLE, S. C, HOLLENBERG, P. F, IANNACCONE, P. M
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page 5633
container_issue 12
container_start_page 5629
container_title Cancer research (Chicago, Ill.)
container_volume 44
creator BARCH, D. H
KUEMMERLE, S. C
HOLLENBERG, P. F
IANNACCONE, P. M
description Epidemiological studies in China suggest that dietary zinc deficiency and environmental exposure to N-nitrosamine carcinogens, such as N-nitrosomethylbenzylamine (NMBA), are among the factors associated with an increased incidence of esophageal carcinoma in humans. NMBA belongs to a class of nitrosamines which require activation by the cytochrome P-450-dependent mixed-function oxidases in order to be mutagenic. Rats maintained on a zinc-deficient diet exhibited an increased incidence of NMBA-induced esophageal carcinoma when compared to rats on a control diet. The increased tumor formation was associated with an alteration of the microsomal metabolism of NMBA. Weanling male Sprague-Dawley rats were raised on egg protein diets containing 2.3 ppm zinc (low zinc) or 50 ppm zinc (control zinc). Analysis of tissues revealed a rapid decline in the levels of zinc in serum and esophagi of the animals fed the low-zinc diet. Gastric and hepatic zinc content did not differ significantly between the animals fed the low-zinc diet and the animals fed the control zinc diet, even after 6 weeks. Microsomes were prepared from esophageal mucosa, livers, and forestomachs from weanling animals fed these diets for 3 weeks. The rate of formation of benzaldehyde from NMBA by esophageal mucosal microsomes prepared from the rats fed the low-zinc diet was nearly 10-fold higher than that of the rats fed the control zinc diet [0.230 +/- 0.047 (S.E.) versus 0.024 +/- 0.008 nmol/min/mg microsomal protein; p less than 0.001]. The rate of benzaldehyde formation by hepatic microsomes was 0.062 +/- 0.005 nmol/min/mg microsomal protein in the rats fed the low-zinc diet and 0.042 +/- 0.002 nmol/min/mg microsomal protein in the rats fed the control zinc diet (p less than 0.01). The rate of benzaldehyde formation by forestomach microsomes was not detectable in tissue from rats on either diet. This increased rate of NMBA metabolism by esophageal mucosal microsomes from the zinc-deficient rats may explain the increased incidence of esophageal carcinoma in these animals.
format Article
fullrecord <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_proquest_miscellaneous_14050293</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>14050293</sourcerecordid><originalsourceid>FETCH-LOGICAL-h307t-14fad499770e58b5f14f2c9838ed67d00e8808c92196c31585f80f7369d57eaa3</originalsourceid><addsrcrecordid>eNo9kE1LxDAQhoMo67r6E4QexFsgaZImOcqyfsCiFwVvJU0nNpKma5M97P56qxZPM-88D-9hTtCSCqaw5FycoiUhRGHBZXmOLlL6nKKgRCzQouJaqZIt0fsmDbvOfIAJRe_tOKSh_1khm2YIPvXF4IpnHH3-RZC7Q2ggHg_B9D5C4WOROyiOPlrcgvPWQ8zFaPIlOnMmJLia5wq93W9e1494-_LwtL7b4o4RmTHlzrRcaykJCNUINx1KqxVT0FayJQSUIsrqkurKMiqUcIo4ySrdCgnGsBW6_evdjcPXHlKue58shGAiDPtUU04EKTWbxOtZ3Dc9tPVu9L0ZD_X8ionfzNwka4IbTbQ-_WuaypIRwb4BVKdovA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>14050293</pqid></control><display><type>article</type><title>Esophageal microsomal metabolism of N-nitrosomethylbenzylamine in the zinc-deficient rat</title><source>MEDLINE</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>American Association for Cancer Research</source><creator>BARCH, D. H ; KUEMMERLE, S. C ; HOLLENBERG, P. F ; IANNACCONE, P. M</creator><creatorcontrib>BARCH, D. H ; KUEMMERLE, S. C ; HOLLENBERG, P. F ; IANNACCONE, P. M</creatorcontrib><description>Epidemiological studies in China suggest that dietary zinc deficiency and environmental exposure to N-nitrosamine carcinogens, such as N-nitrosomethylbenzylamine (NMBA), are among the factors associated with an increased incidence of esophageal carcinoma in humans. NMBA belongs to a class of nitrosamines which require activation by the cytochrome P-450-dependent mixed-function oxidases in order to be mutagenic. Rats maintained on a zinc-deficient diet exhibited an increased incidence of NMBA-induced esophageal carcinoma when compared to rats on a control diet. The increased tumor formation was associated with an alteration of the microsomal metabolism of NMBA. Weanling male Sprague-Dawley rats were raised on egg protein diets containing 2.3 ppm zinc (low zinc) or 50 ppm zinc (control zinc). Analysis of tissues revealed a rapid decline in the levels of zinc in serum and esophagi of the animals fed the low-zinc diet. Gastric and hepatic zinc content did not differ significantly between the animals fed the low-zinc diet and the animals fed the control zinc diet, even after 6 weeks. Microsomes were prepared from esophageal mucosa, livers, and forestomachs from weanling animals fed these diets for 3 weeks. The rate of formation of benzaldehyde from NMBA by esophageal mucosal microsomes prepared from the rats fed the low-zinc diet was nearly 10-fold higher than that of the rats fed the control zinc diet [0.230 +/- 0.047 (S.E.) versus 0.024 +/- 0.008 nmol/min/mg microsomal protein; p less than 0.001]. The rate of benzaldehyde formation by hepatic microsomes was 0.062 +/- 0.005 nmol/min/mg microsomal protein in the rats fed the low-zinc diet and 0.042 +/- 0.002 nmol/min/mg microsomal protein in the rats fed the control zinc diet (p less than 0.01). The rate of benzaldehyde formation by forestomach microsomes was not detectable in tissue from rats on either diet. This increased rate of NMBA metabolism by esophageal mucosal microsomes from the zinc-deficient rats may explain the increased incidence of esophageal carcinoma in these animals.</description><identifier>ISSN: 0008-5472</identifier><identifier>EISSN: 1538-7445</identifier><identifier>PMID: 6498823</identifier><identifier>CODEN: CNREA8</identifier><language>eng</language><publisher>Philadelphia, PA: American Association for Cancer Research</publisher><subject>Animals ; Biological and medical sciences ; Carcinogenesis, carcinogens and anticarcinogens ; Carcinogens - metabolism ; Chemical agents ; Diet ; Dimethylnitrosamine - analogs &amp; derivatives ; Dimethylnitrosamine - metabolism ; Dimethylnitrosamine - toxicity ; Esophageal Neoplasms - chemically induced ; Esophageal Neoplasms - pathology ; Esophagus - metabolism ; Male ; Medical sciences ; Microsomes - metabolism ; Rats ; Rats, Inbred Strains ; Tumors ; Zinc - analysis ; Zinc - deficiency</subject><ispartof>Cancer research (Chicago, Ill.), 1984-12, Vol.44 (12), p.5629-5633</ispartof><rights>1985 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&amp;idt=9172305$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/6498823$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>BARCH, D. H</creatorcontrib><creatorcontrib>KUEMMERLE, S. C</creatorcontrib><creatorcontrib>HOLLENBERG, P. F</creatorcontrib><creatorcontrib>IANNACCONE, P. M</creatorcontrib><title>Esophageal microsomal metabolism of N-nitrosomethylbenzylamine in the zinc-deficient rat</title><title>Cancer research (Chicago, Ill.)</title><addtitle>Cancer Res</addtitle><description>Epidemiological studies in China suggest that dietary zinc deficiency and environmental exposure to N-nitrosamine carcinogens, such as N-nitrosomethylbenzylamine (NMBA), are among the factors associated with an increased incidence of esophageal carcinoma in humans. NMBA belongs to a class of nitrosamines which require activation by the cytochrome P-450-dependent mixed-function oxidases in order to be mutagenic. Rats maintained on a zinc-deficient diet exhibited an increased incidence of NMBA-induced esophageal carcinoma when compared to rats on a control diet. The increased tumor formation was associated with an alteration of the microsomal metabolism of NMBA. Weanling male Sprague-Dawley rats were raised on egg protein diets containing 2.3 ppm zinc (low zinc) or 50 ppm zinc (control zinc). Analysis of tissues revealed a rapid decline in the levels of zinc in serum and esophagi of the animals fed the low-zinc diet. Gastric and hepatic zinc content did not differ significantly between the animals fed the low-zinc diet and the animals fed the control zinc diet, even after 6 weeks. Microsomes were prepared from esophageal mucosa, livers, and forestomachs from weanling animals fed these diets for 3 weeks. The rate of formation of benzaldehyde from NMBA by esophageal mucosal microsomes prepared from the rats fed the low-zinc diet was nearly 10-fold higher than that of the rats fed the control zinc diet [0.230 +/- 0.047 (S.E.) versus 0.024 +/- 0.008 nmol/min/mg microsomal protein; p less than 0.001]. The rate of benzaldehyde formation by hepatic microsomes was 0.062 +/- 0.005 nmol/min/mg microsomal protein in the rats fed the low-zinc diet and 0.042 +/- 0.002 nmol/min/mg microsomal protein in the rats fed the control zinc diet (p less than 0.01). The rate of benzaldehyde formation by forestomach microsomes was not detectable in tissue from rats on either diet. This increased rate of NMBA metabolism by esophageal mucosal microsomes from the zinc-deficient rats may explain the increased incidence of esophageal carcinoma in these animals.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Carcinogenesis, carcinogens and anticarcinogens</subject><subject>Carcinogens - metabolism</subject><subject>Chemical agents</subject><subject>Diet</subject><subject>Dimethylnitrosamine - analogs &amp; derivatives</subject><subject>Dimethylnitrosamine - metabolism</subject><subject>Dimethylnitrosamine - toxicity</subject><subject>Esophageal Neoplasms - chemically induced</subject><subject>Esophageal Neoplasms - pathology</subject><subject>Esophagus - metabolism</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Microsomes - metabolism</subject><subject>Rats</subject><subject>Rats, Inbred Strains</subject><subject>Tumors</subject><subject>Zinc - analysis</subject><subject>Zinc - deficiency</subject><issn>0008-5472</issn><issn>1538-7445</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1984</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kE1LxDAQhoMo67r6E4QexFsgaZImOcqyfsCiFwVvJU0nNpKma5M97P56qxZPM-88D-9hTtCSCqaw5FycoiUhRGHBZXmOLlL6nKKgRCzQouJaqZIt0fsmDbvOfIAJRe_tOKSh_1khm2YIPvXF4IpnHH3-RZC7Q2ggHg_B9D5C4WOROyiOPlrcgvPWQ8zFaPIlOnMmJLia5wq93W9e1494-_LwtL7b4o4RmTHlzrRcaykJCNUINx1KqxVT0FayJQSUIsrqkurKMiqUcIo4ySrdCgnGsBW6_evdjcPXHlKue58shGAiDPtUU04EKTWbxOtZ3Dc9tPVu9L0ZD_X8ionfzNwka4IbTbQ-_WuaypIRwb4BVKdovA</recordid><startdate>19841201</startdate><enddate>19841201</enddate><creator>BARCH, D. H</creator><creator>KUEMMERLE, S. C</creator><creator>HOLLENBERG, P. F</creator><creator>IANNACCONE, P. M</creator><general>American Association for Cancer Research</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7U7</scope><scope>C1K</scope></search><sort><creationdate>19841201</creationdate><title>Esophageal microsomal metabolism of N-nitrosomethylbenzylamine in the zinc-deficient rat</title><author>BARCH, D. H ; KUEMMERLE, S. C ; HOLLENBERG, P. F ; IANNACCONE, P. M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-h307t-14fad499770e58b5f14f2c9838ed67d00e8808c92196c31585f80f7369d57eaa3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1984</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Carcinogenesis, carcinogens and anticarcinogens</topic><topic>Carcinogens - metabolism</topic><topic>Chemical agents</topic><topic>Diet</topic><topic>Dimethylnitrosamine - analogs &amp; derivatives</topic><topic>Dimethylnitrosamine - metabolism</topic><topic>Dimethylnitrosamine - toxicity</topic><topic>Esophageal Neoplasms - chemically induced</topic><topic>Esophageal Neoplasms - pathology</topic><topic>Esophagus - metabolism</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Microsomes - metabolism</topic><topic>Rats</topic><topic>Rats, Inbred Strains</topic><topic>Tumors</topic><topic>Zinc - analysis</topic><topic>Zinc - deficiency</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>BARCH, D. H</creatorcontrib><creatorcontrib>KUEMMERLE, S. C</creatorcontrib><creatorcontrib>HOLLENBERG, P. F</creatorcontrib><creatorcontrib>IANNACCONE, P. M</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>Toxicology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><jtitle>Cancer research (Chicago, Ill.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>BARCH, D. H</au><au>KUEMMERLE, S. C</au><au>HOLLENBERG, P. F</au><au>IANNACCONE, P. M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Esophageal microsomal metabolism of N-nitrosomethylbenzylamine in the zinc-deficient rat</atitle><jtitle>Cancer research (Chicago, Ill.)</jtitle><addtitle>Cancer Res</addtitle><date>1984-12-01</date><risdate>1984</risdate><volume>44</volume><issue>12</issue><spage>5629</spage><epage>5633</epage><pages>5629-5633</pages><issn>0008-5472</issn><eissn>1538-7445</eissn><coden>CNREA8</coden><abstract>Epidemiological studies in China suggest that dietary zinc deficiency and environmental exposure to N-nitrosamine carcinogens, such as N-nitrosomethylbenzylamine (NMBA), are among the factors associated with an increased incidence of esophageal carcinoma in humans. NMBA belongs to a class of nitrosamines which require activation by the cytochrome P-450-dependent mixed-function oxidases in order to be mutagenic. Rats maintained on a zinc-deficient diet exhibited an increased incidence of NMBA-induced esophageal carcinoma when compared to rats on a control diet. The increased tumor formation was associated with an alteration of the microsomal metabolism of NMBA. Weanling male Sprague-Dawley rats were raised on egg protein diets containing 2.3 ppm zinc (low zinc) or 50 ppm zinc (control zinc). Analysis of tissues revealed a rapid decline in the levels of zinc in serum and esophagi of the animals fed the low-zinc diet. Gastric and hepatic zinc content did not differ significantly between the animals fed the low-zinc diet and the animals fed the control zinc diet, even after 6 weeks. Microsomes were prepared from esophageal mucosa, livers, and forestomachs from weanling animals fed these diets for 3 weeks. The rate of formation of benzaldehyde from NMBA by esophageal mucosal microsomes prepared from the rats fed the low-zinc diet was nearly 10-fold higher than that of the rats fed the control zinc diet [0.230 +/- 0.047 (S.E.) versus 0.024 +/- 0.008 nmol/min/mg microsomal protein; p less than 0.001]. The rate of benzaldehyde formation by hepatic microsomes was 0.062 +/- 0.005 nmol/min/mg microsomal protein in the rats fed the low-zinc diet and 0.042 +/- 0.002 nmol/min/mg microsomal protein in the rats fed the control zinc diet (p less than 0.01). The rate of benzaldehyde formation by forestomach microsomes was not detectable in tissue from rats on either diet. This increased rate of NMBA metabolism by esophageal mucosal microsomes from the zinc-deficient rats may explain the increased incidence of esophageal carcinoma in these animals.</abstract><cop>Philadelphia, PA</cop><pub>American Association for Cancer Research</pub><pmid>6498823</pmid><tpages>5</tpages></addata></record>
fulltext fulltext
identifier ISSN: 0008-5472
ispartof Cancer research (Chicago, Ill.), 1984-12, Vol.44 (12), p.5629-5633
issn 0008-5472
1538-7445
language eng
recordid cdi_proquest_miscellaneous_14050293
source MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; American Association for Cancer Research
subjects Animals
Biological and medical sciences
Carcinogenesis, carcinogens and anticarcinogens
Carcinogens - metabolism
Chemical agents
Diet
Dimethylnitrosamine - analogs & derivatives
Dimethylnitrosamine - metabolism
Dimethylnitrosamine - toxicity
Esophageal Neoplasms - chemically induced
Esophageal Neoplasms - pathology
Esophagus - metabolism
Male
Medical sciences
Microsomes - metabolism
Rats
Rats, Inbred Strains
Tumors
Zinc - analysis
Zinc - deficiency
title Esophageal microsomal metabolism of N-nitrosomethylbenzylamine in the zinc-deficient rat
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-23T06%3A05%3A20IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Esophageal%20microsomal%20metabolism%20of%20N-nitrosomethylbenzylamine%20in%20the%20zinc-deficient%20rat&rft.jtitle=Cancer%20research%20(Chicago,%20Ill.)&rft.au=BARCH,%20D.%20H&rft.date=1984-12-01&rft.volume=44&rft.issue=12&rft.spage=5629&rft.epage=5633&rft.pages=5629-5633&rft.issn=0008-5472&rft.eissn=1538-7445&rft.coden=CNREA8&rft_id=info:doi/&rft_dat=%3Cproquest_pubme%3E14050293%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=14050293&rft_id=info:pmid/6498823&rfr_iscdi=true