Juvenile hormone and 20-hydroxyecdysone as primary and secondary stimuli of vitellogenesis in Aedes aegypti

Female Aedes aegypti that were fed blood and immediately abdominally ligated did not deposit yolk. Injection of 20‐hydroxyecdysone (1.5–5.0 ng) or topical application of juvenile hormone (JH) analogue methoprene (25 pg) did not induce vitellogenesis in these abdomens. When blood‐gorged ligated abdom...

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Veröffentlicht in:Archives of insect biochemistry and physiology 1985, Vol.2 (1), p.75-90
Hauptverfasser: Borovsky, D, Thomas, B.R, Carlson, D.A, Whisenton, L.R, Fuchs, M.S
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container_issue 1
container_start_page 75
container_title Archives of insect biochemistry and physiology
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creator Borovsky, D
Thomas, B.R
Carlson, D.A
Whisenton, L.R
Fuchs, M.S
description Female Aedes aegypti that were fed blood and immediately abdominally ligated did not deposit yolk. Injection of 20‐hydroxyecdysone (1.5–5.0 ng) or topical application of juvenile hormone (JH) analogue methoprene (25 pg) did not induce vitellogenesis in these abdomens. When blood‐gorged ligated abdomens were treated with both hormones, however, vitellogenesis was stimulated in 60% of treated animals. Rocket immunoelectrophoresis indicated that vitellin concentration per follicle in treated animals was similar to that in intact controls. When ligated abdomens were first treated with methoprene and immediately injected with a crude head extract of egg development neurosecretory hormone, vitellogenin synthesis was induced at a rate similar to that in blood‐fed controls. Methoprene at this concentration (25 pg), did not cause an increase in whole‐body ecdysteroid titers. Larger amounts of methoprene (1.65 ng) were needed to stimulate egg development and ecdysteroid production. Implantation of ecdysone‐secreting ovaries into ligated abdomens did not stimulate vitellogenesis in the recipients. However, in recipients that were first treated with methoprene (25 pg), implantation of ecdysone‐secreting ovaries resulted in normal egg development. These experiments indicate that the appearance of JH precedes 20‐hydroxyecdysone in stimulating vitellogenesis following blood feeding in Ae. aegypti.
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Insect Biochem. Physiol</addtitle><description>Female Aedes aegypti that were fed blood and immediately abdominally ligated did not deposit yolk. Injection of 20‐hydroxyecdysone (1.5–5.0 ng) or topical application of juvenile hormone (JH) analogue methoprene (25 pg) did not induce vitellogenesis in these abdomens. When blood‐gorged ligated abdomens were treated with both hormones, however, vitellogenesis was stimulated in 60% of treated animals. Rocket immunoelectrophoresis indicated that vitellin concentration per follicle in treated animals was similar to that in intact controls. When ligated abdomens were first treated with methoprene and immediately injected with a crude head extract of egg development neurosecretory hormone, vitellogenin synthesis was induced at a rate similar to that in blood‐fed controls. Methoprene at this concentration (25 pg), did not cause an increase in whole‐body ecdysteroid titers. 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Physiology. Immunology</topic><topic>Biological and medical sciences</topic><topic>Culicidae</topic><topic>CULTIVO DE TEJIDOS</topic><topic>CULTURE DE TISSUS</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>HORMONAS</topic><topic>HORMONAS DE LA MUDA</topic><topic>HORMONE</topic><topic>HORMONE DE MUE</topic><topic>HORMONES</topic><topic>Insecta</topic><topic>Invertebrates</topic><topic>juvenile hormones</topic><topic>MOULTING HORMONES</topic><topic>Physiology. Development</topic><topic>TISSUE CULTURE</topic><topic>vitellogenesis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Borovsky, D</creatorcontrib><creatorcontrib>Thomas, B.R</creatorcontrib><creatorcontrib>Carlson, D.A</creatorcontrib><creatorcontrib>Whisenton, L.R</creatorcontrib><creatorcontrib>Fuchs, M.S</creatorcontrib><collection>AGRIS</collection><collection>Istex</collection><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>Endocrinology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><jtitle>Archives of insect biochemistry and physiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Borovsky, D</au><au>Thomas, B.R</au><au>Carlson, D.A</au><au>Whisenton, L.R</au><au>Fuchs, M.S</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Juvenile hormone and 20-hydroxyecdysone as primary and secondary stimuli of vitellogenesis in Aedes aegypti</atitle><jtitle>Archives of insect biochemistry and physiology</jtitle><addtitle>Arch. 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subjects 20-hydroxyecdysone
AEDES
AEDES AEGYPTI
Biochemistry. Physiology. Immunology
Biological and medical sciences
Culicidae
CULTIVO DE TEJIDOS
CULTURE DE TISSUS
Fundamental and applied biological sciences. Psychology
HORMONAS
HORMONAS DE LA MUDA
HORMONE
HORMONE DE MUE
HORMONES
Insecta
Invertebrates
juvenile hormones
MOULTING HORMONES
Physiology. Development
TISSUE CULTURE
vitellogenesis
title Juvenile hormone and 20-hydroxyecdysone as primary and secondary stimuli of vitellogenesis in Aedes aegypti
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