Copper toxicity in Withania somnifera: Growth and antioxidant enzymes responses of in vitro grown plants

Indian ginseng ( Withania somnifera L. Dunal), a medicinally important plant exposed to different concentrations of CuSO 4 (0, 10, 25, 50, 100 and 200 μM) for 30 d in vitro accumulated high amount of Cu in a concentration manner. Toxic effects of Cu on plants were reflected by the reductions in fresh weight (FW), shoot and root length, chlorophyll and carotenoids contents. Malondialdehyde (MDA), a decomposition product of polyunsaturated fatty acids hydroperoxides, often considered as a suitable biomarker for lipid peroxidation was induced in metal-treated plants compared to the control. This hypothesis is also confirmed by the higher lipoxygenase (LOX) activity, coupled with higher hydrogen peroxide (H 2O 2) and superoxide anions (O 2 − ) content in treated plants. Ascorbate peroxidase (APX), monodehydroascorbate reductase (MDHAR), dehydroascorbate reductase (DHAR), glutathione- S-transferase (GST) and guaiacol-peroxidase (G-POD) activities of leaves were increased in the presence of Cu when compared to control plants indicated that antioxidant enzymes played an important role in protecting the plant from Cu toxicity. After native polyacrylamide gel electrophoresis (PAGE) analysis, six APX and four G-POD isoforms were detected and significantly induced in metal-treated plants as compared to control plants. The marked decrease in superoxide dismutase (SOD), catalase (CAT), glutathione reductase (GR) and glutathione peroxidase (GPx) activities seems to reflect its inability for eliminating the reactive oxygen species (ROS) resulting from Cu-induced oxidative stress. On the other hand, total phenolic contents increased with increasing concentration of Cu compared to the control. This study reveals that plants have the ability to grow in Cu polluted areas by altering various physiological changes. Since the level of total phenolic content in metal-treated plants increases, therefore, it is advisable to assess the biological activity of the plants before using for medicinal purposes.