Soil Metabolism of [14C]Methiozolin under Aerobic and Anaerobic Flooded Conditions

Methiozolin is a new turf herbicide controlling annual bluegrass in various cool- and warm-season turfgrasses. This study was conducted to investigate the fate of methiozolin in soil under aerobic and anaerobic flooded conditions using two radiolabeled tracers, [benzyl-14C]- and [isoxazole-14C]­methiozolin. The mass balance of applied radioactivity ranged from 91.7 to 104.5% in both soil conditions. In the soil under the aerobic condition, [14C]­methiozolin degraded with time to remain by 17.9 and 15.9% of the applied in soil at 120 days after treatment (DAT). [14C]Carbon dioxide and the nonextractable radioactivity increased as the soil aged to reach up to 41.5 and 35.7% for [benzyl-14C]­methiozolin at 120 DAT, respectively, but 36.1 and 39.8% for [isoxazole-14C]­methiozolin, respectively, during the same period. The nonextractable residue was associated more with humin and fulvic acid fractions under the aerobic condition. No significant volatile products or metabolites were detected during this study. The half-life of [14C]­methiozolin was approximately 49 days in the soil under the aerobic condition; however, it could not be estimated in the soil under the anaerobic flooded condition because [14C]­methiozolin degradation was limited. On the basis of these results, methiozolin is considered to undergo fast degradation by aerobic microbes, but not by anaerobic microbes in soil.