Cotton plasma membrane intrinsic protein 2s (PIP2s) selectively interact to regulate their water channel activities and are required for fibre development
Aquaporins are thought to be associated with water transport and play important roles in cotton (Gossypium hirsutum) fibre elongation. Among aquaporins, plasma membrane intrinsic proteins (PIPs) constitute a plasma-membrane-specific subfamily and are further subdivided into PIP1 and PIP2 groups. In...
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| Veröffentlicht in: | The New phytologist 2013-08, Vol.199 (3), p.695-707 |
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| container_title | The New phytologist |
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| creator | Li, Deng‐Di Ruan, Xiang‐Mei Zhang, Jie Wu, Ya‐Jie Wang, Xiu‐Lan Li, Xue‐Bao |
| description | Aquaporins are thought to be associated with water transport and play important roles in cotton (Gossypium hirsutum) fibre elongation. Among aquaporins, plasma membrane intrinsic proteins (PIPs) constitute a plasma-membrane-specific subfamily and are further subdivided into PIP1 and PIP2 groups.
In this study, four fibre-preferential GhPIP2 genes were functionally characterized. The selective interactions among GhPIP2s and their interaction proteins were studied in detail to elucidate the molecular mechanism of cotton fibre development.
GhPIP2;3 interacted with GhPIP2;4 and GhPIP2;6, but GhPIP2;6 did not interact with GhPIP2;4. Coexpression of GhPIP2;3/2;4 or GhPIP2;3/2;6 resulted in a positive cooperative effect which increased the permeability coefficient of oocytes, while GhPIP2;4/2;6 did not. GhBCP2 (a blue copper-binding protein) inhibited GhPIP2;6 water channel activity through their interaction. Overexpression of GhPIP2 genes in yeast induced longitudinal growth of the host cells. By contrast, knockdown of expression of GhPIP2 genes in cotton by RNA interference markedly hindered fibre elongation.
In conclusion, GhPIP2 proteins are the primary aquaporin isoforms in fibres. They selectively form hetero-oligomers in order to regulate their activities to meet the requirements for rapid fibre elongation. |
| doi_str_mv | 10.1111/nph.12309 |
| format | Article |
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In this study, four fibre-preferential GhPIP2 genes were functionally characterized. The selective interactions among GhPIP2s and their interaction proteins were studied in detail to elucidate the molecular mechanism of cotton fibre development.
GhPIP2;3 interacted with GhPIP2;4 and GhPIP2;6, but GhPIP2;6 did not interact with GhPIP2;4. Coexpression of GhPIP2;3/2;4 or GhPIP2;3/2;6 resulted in a positive cooperative effect which increased the permeability coefficient of oocytes, while GhPIP2;4/2;6 did not. GhBCP2 (a blue copper-binding protein) inhibited GhPIP2;6 water channel activity through their interaction. Overexpression of GhPIP2 genes in yeast induced longitudinal growth of the host cells. By contrast, knockdown of expression of GhPIP2 genes in cotton by RNA interference markedly hindered fibre elongation.
In conclusion, GhPIP2 proteins are the primary aquaporin isoforms in fibres. They selectively form hetero-oligomers in order to regulate their activities to meet the requirements for rapid fibre elongation.</description><identifier>ISSN: 0028-646X</identifier><identifier>EISSN: 1469-8137</identifier><identifier>DOI: 10.1111/nph.12309</identifier><identifier>PMID: 23656428</identifier><language>eng</language><publisher>England: New Phytologist Trust</publisher><subject>Animals ; aquaporin ; Aquaporins ; Aquaporins - genetics ; Aquaporins - metabolism ; Cell Membrane - metabolism ; Cell membranes ; coexpression ; Cotton ; cotton (Gossypium hirsutum) ; Cotton Fiber ; Cotton fibers ; Down-Regulation ; Fiber cells ; fibre development ; Fluorescence ; Gene Expression Regulation, Plant ; Gene Knockdown Techniques ; Genes, Plant - genetics ; Genetic vectors ; Gossypium - genetics ; Gossypium - metabolism ; Oocytes ; Oocytes - metabolism ; Plant Proteins - genetics ; Plant Proteins - metabolism ; Plants ; Plasma interactions ; Protein Binding ; protein–protein interaction ; Reverse Transcriptase Polymerase Chain Reaction ; RNA Interference ; Schizosaccharomyces - genetics ; Subcellular Fractions - metabolism ; suppression of gene expression ; Two-Hybrid System Techniques ; Xenopus laevis - metabolism ; Yeasts</subject><ispartof>The New phytologist, 2013-08, Vol.199 (3), p.695-707</ispartof><rights>2013 New Phytologist Trust</rights><rights>2013 The Authors. New Phytologist © 2013 New Phytologist Trust</rights><rights>2013 The Authors. New Phytologist © 2013 New Phytologist Trust.</rights><rights>Copyright © 2013 New Phytologist Trust</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4599-92cf19cca6eb85f01172b5bf231e5af591327159c817a11a955b9b5600a5a2853</citedby><cites>FETCH-LOGICAL-c4599-92cf19cca6eb85f01172b5bf231e5af591327159c817a11a955b9b5600a5a2853</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/newphytologist.199.3.695$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/newphytologist.199.3.695$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,778,782,801,1414,1430,27907,27908,45557,45558,46392,46816,58000,58233</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23656428$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Li, Deng‐Di</creatorcontrib><creatorcontrib>Ruan, Xiang‐Mei</creatorcontrib><creatorcontrib>Zhang, Jie</creatorcontrib><creatorcontrib>Wu, Ya‐Jie</creatorcontrib><creatorcontrib>Wang, Xiu‐Lan</creatorcontrib><creatorcontrib>Li, Xue‐Bao</creatorcontrib><title>Cotton plasma membrane intrinsic protein 2s (PIP2s) selectively interact to regulate their water channel activities and are required for fibre development</title><title>The New phytologist</title><addtitle>New Phytol</addtitle><description>Aquaporins are thought to be associated with water transport and play important roles in cotton (Gossypium hirsutum) fibre elongation. Among aquaporins, plasma membrane intrinsic proteins (PIPs) constitute a plasma-membrane-specific subfamily and are further subdivided into PIP1 and PIP2 groups.
In this study, four fibre-preferential GhPIP2 genes were functionally characterized. The selective interactions among GhPIP2s and their interaction proteins were studied in detail to elucidate the molecular mechanism of cotton fibre development.
GhPIP2;3 interacted with GhPIP2;4 and GhPIP2;6, but GhPIP2;6 did not interact with GhPIP2;4. Coexpression of GhPIP2;3/2;4 or GhPIP2;3/2;6 resulted in a positive cooperative effect which increased the permeability coefficient of oocytes, while GhPIP2;4/2;6 did not. GhBCP2 (a blue copper-binding protein) inhibited GhPIP2;6 water channel activity through their interaction. Overexpression of GhPIP2 genes in yeast induced longitudinal growth of the host cells. By contrast, knockdown of expression of GhPIP2 genes in cotton by RNA interference markedly hindered fibre elongation.
In conclusion, GhPIP2 proteins are the primary aquaporin isoforms in fibres. They selectively form hetero-oligomers in order to regulate their activities to meet the requirements for rapid fibre elongation.</description><subject>Animals</subject><subject>aquaporin</subject><subject>Aquaporins</subject><subject>Aquaporins - genetics</subject><subject>Aquaporins - metabolism</subject><subject>Cell Membrane - metabolism</subject><subject>Cell membranes</subject><subject>coexpression</subject><subject>Cotton</subject><subject>cotton (Gossypium hirsutum)</subject><subject>Cotton Fiber</subject><subject>Cotton fibers</subject><subject>Down-Regulation</subject><subject>Fiber cells</subject><subject>fibre development</subject><subject>Fluorescence</subject><subject>Gene Expression Regulation, Plant</subject><subject>Gene Knockdown Techniques</subject><subject>Genes, Plant - genetics</subject><subject>Genetic vectors</subject><subject>Gossypium - genetics</subject><subject>Gossypium - metabolism</subject><subject>Oocytes</subject><subject>Oocytes - metabolism</subject><subject>Plant Proteins - genetics</subject><subject>Plant Proteins - metabolism</subject><subject>Plants</subject><subject>Plasma interactions</subject><subject>Protein Binding</subject><subject>protein–protein interaction</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>RNA Interference</subject><subject>Schizosaccharomyces - genetics</subject><subject>Subcellular Fractions - metabolism</subject><subject>suppression of gene expression</subject><subject>Two-Hybrid System Techniques</subject><subject>Xenopus laevis - metabolism</subject><subject>Yeasts</subject><issn>0028-646X</issn><issn>1469-8137</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kU9vFCEYh4nR2G314BcwJF7aw7T8GWaGo9lo26TRPWjijTDsO102DEyB6Wa_ip9W1m17MJELhDy_Jy_8EPpAySUt68pPm0vKOJGv0ILWjaw6ytvXaEEI66qmbn6doNOUtoQQKRr2Fp0w3oimZt0C_V6GnIPHk9Np1HiEsY_aA7Y-R-uTNXiKIYP1mCV8vrpdsXSBEzgw2T6C2x9AiNpknAOOcD87nQHnDdiId-UYsdlo78FhfUjYbCFh7ddYRyj8w2wjrPEQIh5sX67WUKxhGsHnd-jNoF2C90_7Gfr59cuP5U119_36dvn5rjK1kLKSzAxUGqMb6DsxEEpb1ot-YJyC0IOQlLOWCmk62mpKtRSil71oCNFCs07wM3R-9JaXPsyQshptMuBc-YcwJ0W5lJIzKkhBP_2DbsMcfZlO0Zq1naibWhbq4kiZGFKKMKgp2lHHvaJEHQpTpTD1t7DCfnwyzv0I6xfyuaECXB2BnXWw_79JfVvdPCurY2KbcogvCQ-7abPPwYV7WwanUiquGin4HzvqsY0</recordid><startdate>201308</startdate><enddate>201308</enddate><creator>Li, Deng‐Di</creator><creator>Ruan, Xiang‐Mei</creator><creator>Zhang, Jie</creator><creator>Wu, Ya‐Jie</creator><creator>Wang, Xiu‐Lan</creator><creator>Li, Xue‐Bao</creator><general>New Phytologist Trust</general><general>Wiley Subscription Services, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7SN</scope><scope>8FD</scope><scope>C1K</scope><scope>F1W</scope><scope>FR3</scope><scope>H95</scope><scope>L.G</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>201308</creationdate><title>Cotton plasma membrane intrinsic protein 2s (PIP2s) selectively interact to regulate their water channel activities and are required for fibre development</title><author>Li, Deng‐Di ; Ruan, Xiang‐Mei ; Zhang, Jie ; Wu, Ya‐Jie ; Wang, Xiu‐Lan ; Li, Xue‐Bao</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4599-92cf19cca6eb85f01172b5bf231e5af591327159c817a11a955b9b5600a5a2853</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Animals</topic><topic>aquaporin</topic><topic>Aquaporins</topic><topic>Aquaporins - genetics</topic><topic>Aquaporins - metabolism</topic><topic>Cell Membrane - metabolism</topic><topic>Cell membranes</topic><topic>coexpression</topic><topic>Cotton</topic><topic>cotton (Gossypium hirsutum)</topic><topic>Cotton Fiber</topic><topic>Cotton fibers</topic><topic>Down-Regulation</topic><topic>Fiber cells</topic><topic>fibre development</topic><topic>Fluorescence</topic><topic>Gene Expression Regulation, Plant</topic><topic>Gene Knockdown Techniques</topic><topic>Genes, Plant - genetics</topic><topic>Genetic vectors</topic><topic>Gossypium - genetics</topic><topic>Gossypium - metabolism</topic><topic>Oocytes</topic><topic>Oocytes - metabolism</topic><topic>Plant Proteins - genetics</topic><topic>Plant Proteins - metabolism</topic><topic>Plants</topic><topic>Plasma interactions</topic><topic>Protein Binding</topic><topic>protein–protein interaction</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>RNA Interference</topic><topic>Schizosaccharomyces - genetics</topic><topic>Subcellular Fractions - metabolism</topic><topic>suppression of gene expression</topic><topic>Two-Hybrid System Techniques</topic><topic>Xenopus laevis - metabolism</topic><topic>Yeasts</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Li, Deng‐Di</creatorcontrib><creatorcontrib>Ruan, Xiang‐Mei</creatorcontrib><creatorcontrib>Zhang, Jie</creatorcontrib><creatorcontrib>Wu, Ya‐Jie</creatorcontrib><creatorcontrib>Wang, Xiu‐Lan</creatorcontrib><creatorcontrib>Li, Xue‐Bao</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Ecology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Engineering Research Database</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>The New phytologist</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Li, Deng‐Di</au><au>Ruan, Xiang‐Mei</au><au>Zhang, Jie</au><au>Wu, Ya‐Jie</au><au>Wang, Xiu‐Lan</au><au>Li, Xue‐Bao</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cotton plasma membrane intrinsic protein 2s (PIP2s) selectively interact to regulate their water channel activities and are required for fibre development</atitle><jtitle>The New phytologist</jtitle><addtitle>New Phytol</addtitle><date>2013-08</date><risdate>2013</risdate><volume>199</volume><issue>3</issue><spage>695</spage><epage>707</epage><pages>695-707</pages><issn>0028-646X</issn><eissn>1469-8137</eissn><abstract>Aquaporins are thought to be associated with water transport and play important roles in cotton (Gossypium hirsutum) fibre elongation. Among aquaporins, plasma membrane intrinsic proteins (PIPs) constitute a plasma-membrane-specific subfamily and are further subdivided into PIP1 and PIP2 groups.
In this study, four fibre-preferential GhPIP2 genes were functionally characterized. The selective interactions among GhPIP2s and their interaction proteins were studied in detail to elucidate the molecular mechanism of cotton fibre development.
GhPIP2;3 interacted with GhPIP2;4 and GhPIP2;6, but GhPIP2;6 did not interact with GhPIP2;4. Coexpression of GhPIP2;3/2;4 or GhPIP2;3/2;6 resulted in a positive cooperative effect which increased the permeability coefficient of oocytes, while GhPIP2;4/2;6 did not. GhBCP2 (a blue copper-binding protein) inhibited GhPIP2;6 water channel activity through their interaction. Overexpression of GhPIP2 genes in yeast induced longitudinal growth of the host cells. By contrast, knockdown of expression of GhPIP2 genes in cotton by RNA interference markedly hindered fibre elongation.
In conclusion, GhPIP2 proteins are the primary aquaporin isoforms in fibres. They selectively form hetero-oligomers in order to regulate their activities to meet the requirements for rapid fibre elongation.</abstract><cop>England</cop><pub>New Phytologist Trust</pub><pmid>23656428</pmid><doi>10.1111/nph.12309</doi><tpages>13</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete; Jstor Complete Legacy; Wiley Free Content; IngentaConnect Free/Open Access Journals; EZB-FREE-00999 freely available EZB journals |
| subjects | Animals aquaporin Aquaporins Aquaporins - genetics Aquaporins - metabolism Cell Membrane - metabolism Cell membranes coexpression Cotton cotton (Gossypium hirsutum) Cotton Fiber Cotton fibers Down-Regulation Fiber cells fibre development Fluorescence Gene Expression Regulation, Plant Gene Knockdown Techniques Genes, Plant - genetics Genetic vectors Gossypium - genetics Gossypium - metabolism Oocytes Oocytes - metabolism Plant Proteins - genetics Plant Proteins - metabolism Plants Plasma interactions Protein Binding protein–protein interaction Reverse Transcriptase Polymerase Chain Reaction RNA Interference Schizosaccharomyces - genetics Subcellular Fractions - metabolism suppression of gene expression Two-Hybrid System Techniques Xenopus laevis - metabolism Yeasts |
| title | Cotton plasma membrane intrinsic protein 2s (PIP2s) selectively interact to regulate their water channel activities and are required for fibre development |
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