Detection of Pythium aphanidermatum in tomato using loop-mediated isothermal amplification (LAMP) with species-specific primers

A loop-mediated isothermal amplification (LAMP) reaction with a primer set designed from the rDNA ITS sequence of P. aphanidermatum was developed. Results of a specificity test using 57 strains of Pythium spp. indicated that the LAMP assay gave no cross reactions in other 39 Pythium species, 11 stra...

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Veröffentlicht in:	European journal of plant pathology 2013-08, Vol.136 (4), p.689-701
Hauptverfasser:	Fukuta, Shiro, Takahashi, Reiko, Kuroyanagi, Satoru, Miyake, Noriyuki, Nagai, Hirofumi, Suzuki, Hirofumi, Hashizume, Fujio, Tsuji, Tomoko, Taguchi, Hiromi, Watanabe, Hideki, Kageyama, Koji
Format:	Artikel
Sprache:	eng
Schlagworte:	Agriculture Biomedical and Life Sciences Deoxyribonucleic acid detection limit DNA Ecology Genomics Hydroponics internal transcribed spacers Life Sciences Lycopersicon esculentum Pathogens Phytophthora Plant diseases Plant Pathology Plant Sciences Polymerase chain reaction Pythium Pythium aphanidermatum ribosomal DNA roots soil Tomatoes
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