Immune Response Evaluation Through Determination of Type 1, Type 2, and Type 17 Patterns in Patients With Epithelial Ovarian Cancer
Innate and adaptive immune cells secrete different cytokines, which participate through distinct mechanisms in cell-mediated immunity and humoral immune responses. The aim of this study was to evaluate the immune response through analysis of type 1 (Th1), Th2, and Th17 cells in patients with epithel...
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| Veröffentlicht in: | Reproductive sciences (Thousand Oaks, Calif.) Calif.), 2013-07, Vol.20 (7), p.828-837 |
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| creator | Cândido, Eduardo Batista Silva, Luciana Maria Carvalho, Andréa Teixeira Lamaita, Rívia Mara Filho, Roberto Mundim Porto Cota, Bianca Della Croce Vieira Silva-Filho, Agnaldo Lopes da |
| description | Innate and adaptive immune cells secrete different cytokines, which participate through distinct mechanisms in cell-mediated immunity and humoral immune responses. The aim of this study was to evaluate the immune response through analysis of type 1 (Th1), Th2, and Th17 cells in patients with epithelial ovarian cancer (EOC). Our study included 44 patients with EOC (study group) and 32 gynecological patients with no ovarian disease (control group). Fragments of ovarian tissue and blood samples were collected in both groups and aliquots of intracystic fluid and peritoneal fluid were recovered from the EOC patient group. Interleukin (IL)-2/IL-4/IL-6/IL-10/IL-17/tumor necrosis factor (TNF)-α/interferon (IFN)-γ levels were measured by cytometric bead array. Statistical analysis included chi-squared, Student t, Mann-Whitney, Kruskal-Wallis tests, and Cox regression model. Patients with EOC were associated with higher levels of TNF-α/IL-4/IL-6/IL-10 compared to the control group. Both IL-10 and TNF-α concentrations were higher in patients with stage III/IV EOC and also associated with higher levels of cancer antigen 125. Higher Th1-mediated immune response was observed when the cytoreduction was considered optimal. However, patients with EOC with unsatisfactory cytoreductive surgery and undifferentiated tumors were associated with higher concentrations of Th2 cytokines in the 4 sites studied. Higher IL-6/IL-10 and lower IFN-γ concentrations were also associated with a lower overall survival rate in patients with EOC. The EOC group presented a predominantly Th2 response and an immunosuppressant standard and had association between IL-6/IL-10/IFN-γ and prognosis. |
| doi_str_mv | 10.1177/1933719112466299 |
| format | Article |
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The aim of this study was to evaluate the immune response through analysis of type 1 (Th1), Th2, and Th17 cells in patients with epithelial ovarian cancer (EOC). Our study included 44 patients with EOC (study group) and 32 gynecological patients with no ovarian disease (control group). Fragments of ovarian tissue and blood samples were collected in both groups and aliquots of intracystic fluid and peritoneal fluid were recovered from the EOC patient group. Interleukin (IL)-2/IL-4/IL-6/IL-10/IL-17/tumor necrosis factor (TNF)-α/interferon (IFN)-γ levels were measured by cytometric bead array. Statistical analysis included chi-squared, Student t, Mann-Whitney, Kruskal-Wallis tests, and Cox regression model. Patients with EOC were associated with higher levels of TNF-α/IL-4/IL-6/IL-10 compared to the control group. Both IL-10 and TNF-α concentrations were higher in patients with stage III/IV EOC and also associated with higher levels of cancer antigen 125. Higher Th1-mediated immune response was observed when the cytoreduction was considered optimal. However, patients with EOC with unsatisfactory cytoreductive surgery and undifferentiated tumors were associated with higher concentrations of Th2 cytokines in the 4 sites studied. Higher IL-6/IL-10 and lower IFN-γ concentrations were also associated with a lower overall survival rate in patients with EOC. The EOC group presented a predominantly Th2 response and an immunosuppressant standard and had association between IL-6/IL-10/IFN-γ and prognosis.</description><identifier>ISSN: 1933-7191</identifier><identifier>EISSN: 1933-7205</identifier><identifier>DOI: 10.1177/1933719112466299</identifier><identifier>PMID: 23239818</identifier><language>eng</language><publisher>Los Angeles, CA: SAGE Publications</publisher><subject>Adult ; Aged ; Carcinoma, Ovarian Epithelial ; Embryology ; Female ; Humans ; Immunity, Cellular ; Interleukin-1 - biosynthesis ; Interleukin-17 - biosynthesis ; Interleukin-2 - biosynthesis ; Medicine & Public Health ; Middle Aged ; Neoplasms, Glandular and Epithelial - diagnosis ; Neoplasms, Glandular and Epithelial - immunology ; Neoplasms, Glandular and Epithelial - mortality ; Obstetrics/Perinatology/Midwifery ; Original Article ; Ovarian Neoplasms - diagnosis ; Ovarian Neoplasms - immunology ; Ovarian Neoplasms - mortality ; Prospective Studies ; Reproductive Medicine ; Survival Rate - trends ; Th1 Cells - immunology ; Th1 Cells - metabolism ; Th1 Cells - pathology ; Th17 Cells - immunology ; Th17 Cells - metabolism ; Th17 Cells - pathology ; Th2 Cells - immunology ; Th2 Cells - metabolism ; Th2 Cells - pathology</subject><ispartof>Reproductive sciences (Thousand Oaks, Calif.), 2013-07, Vol.20 (7), p.828-837</ispartof><rights>The Author(s) 2012</rights><rights>Society for Reproductive Investigation 2013</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c412t-4af9c9efc445581b563473b048806a95913f63dabbe52ff98cd9e55957098e23</citedby><cites>FETCH-LOGICAL-c412t-4af9c9efc445581b563473b048806a95913f63dabbe52ff98cd9e55957098e23</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://journals.sagepub.com/doi/pdf/10.1177/1933719112466299$$EPDF$$P50$$Gsage$$H</linktopdf><linktohtml>$$Uhttps://journals.sagepub.com/doi/10.1177/1933719112466299$$EHTML$$P50$$Gsage$$H</linktohtml><link.rule.ids>314,780,784,21818,27923,27924,41487,42556,43620,43621,51318</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23239818$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Cândido, Eduardo Batista</creatorcontrib><creatorcontrib>Silva, Luciana Maria</creatorcontrib><creatorcontrib>Carvalho, Andréa Teixeira</creatorcontrib><creatorcontrib>Lamaita, Rívia Mara</creatorcontrib><creatorcontrib>Filho, Roberto Mundim Porto</creatorcontrib><creatorcontrib>Cota, Bianca Della Croce Vieira</creatorcontrib><creatorcontrib>Silva-Filho, Agnaldo Lopes da</creatorcontrib><title>Immune Response Evaluation Through Determination of Type 1, Type 2, and Type 17 Patterns in Patients With Epithelial Ovarian Cancer</title><title>Reproductive sciences (Thousand Oaks, Calif.)</title><addtitle>Reprod. Sci</addtitle><addtitle>Reprod Sci</addtitle><description>Innate and adaptive immune cells secrete different cytokines, which participate through distinct mechanisms in cell-mediated immunity and humoral immune responses. The aim of this study was to evaluate the immune response through analysis of type 1 (Th1), Th2, and Th17 cells in patients with epithelial ovarian cancer (EOC). Our study included 44 patients with EOC (study group) and 32 gynecological patients with no ovarian disease (control group). Fragments of ovarian tissue and blood samples were collected in both groups and aliquots of intracystic fluid and peritoneal fluid were recovered from the EOC patient group. Interleukin (IL)-2/IL-4/IL-6/IL-10/IL-17/tumor necrosis factor (TNF)-α/interferon (IFN)-γ levels were measured by cytometric bead array. Statistical analysis included chi-squared, Student t, Mann-Whitney, Kruskal-Wallis tests, and Cox regression model. Patients with EOC were associated with higher levels of TNF-α/IL-4/IL-6/IL-10 compared to the control group. Both IL-10 and TNF-α concentrations were higher in patients with stage III/IV EOC and also associated with higher levels of cancer antigen 125. Higher Th1-mediated immune response was observed when the cytoreduction was considered optimal. However, patients with EOC with unsatisfactory cytoreductive surgery and undifferentiated tumors were associated with higher concentrations of Th2 cytokines in the 4 sites studied. Higher IL-6/IL-10 and lower IFN-γ concentrations were also associated with a lower overall survival rate in patients with EOC. The EOC group presented a predominantly Th2 response and an immunosuppressant standard and had association between IL-6/IL-10/IFN-γ and prognosis.</description><subject>Adult</subject><subject>Aged</subject><subject>Carcinoma, Ovarian Epithelial</subject><subject>Embryology</subject><subject>Female</subject><subject>Humans</subject><subject>Immunity, Cellular</subject><subject>Interleukin-1 - biosynthesis</subject><subject>Interleukin-17 - biosynthesis</subject><subject>Interleukin-2 - biosynthesis</subject><subject>Medicine & Public Health</subject><subject>Middle Aged</subject><subject>Neoplasms, Glandular and Epithelial - diagnosis</subject><subject>Neoplasms, Glandular and Epithelial - immunology</subject><subject>Neoplasms, Glandular and Epithelial - mortality</subject><subject>Obstetrics/Perinatology/Midwifery</subject><subject>Original Article</subject><subject>Ovarian Neoplasms - diagnosis</subject><subject>Ovarian Neoplasms - immunology</subject><subject>Ovarian Neoplasms - mortality</subject><subject>Prospective Studies</subject><subject>Reproductive Medicine</subject><subject>Survival Rate - trends</subject><subject>Th1 Cells - immunology</subject><subject>Th1 Cells - metabolism</subject><subject>Th1 Cells - pathology</subject><subject>Th17 Cells - immunology</subject><subject>Th17 Cells - metabolism</subject><subject>Th17 Cells - pathology</subject><subject>Th2 Cells - immunology</subject><subject>Th2 Cells - metabolism</subject><subject>Th2 Cells - pathology</subject><issn>1933-7191</issn><issn>1933-7205</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkUFP3DAQha0KVOjSOyfkIwdSPHaceI5ou7RISFTVShwjJzvZNUqcYCdInPvHySpLDz1UXDyjN9-bkfwYOwfxDSDPrwGVygEBZJplEvETO91LSS6FPnrvp_kJ-xLjkxA6RWk-sxOppEID5pT9uWvb0RP_TbHvfCS-erHNaAfXeb7ehW7c7vh3Gii0zs9qV_P1a08cruYqr7j1m4OW8192mGgfufP73pEfIn90w46v-umlxtmGP7zY4KznS-srCmfsuLZNpK-HumDr29V6-TO5f_hxt7y5T6oU5JCktsYKqa7SVGsDpc5UmqtSpMaIzKJGUHWmNrYsScu6RlNtkLRGnQs0JNWCXc5r-9A9jxSHonWxoqaxnroxFqAQETKTiw-gmUZjYLq_YGJGq9DFGKgu-uBaG14LEMU-pOLfkCbLxWH7WLa0-Wt4T2UCYAbiNPJbCsVTNwY__c3_liYHj93SB_g32ASnAw</recordid><startdate>20130701</startdate><enddate>20130701</enddate><creator>Cândido, Eduardo Batista</creator><creator>Silva, Luciana Maria</creator><creator>Carvalho, Andréa Teixeira</creator><creator>Lamaita, Rívia Mara</creator><creator>Filho, Roberto Mundim Porto</creator><creator>Cota, Bianca Della Croce Vieira</creator><creator>Silva-Filho, Agnaldo Lopes da</creator><general>SAGE Publications</general><general>Springer International Publishing</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7T5</scope><scope>H94</scope></search><sort><creationdate>20130701</creationdate><title>Immune Response Evaluation Through Determination of Type 1, Type 2, and Type 17 Patterns in Patients With Epithelial Ovarian Cancer</title><author>Cândido, Eduardo Batista ; Silva, Luciana Maria ; Carvalho, Andréa Teixeira ; Lamaita, Rívia Mara ; Filho, Roberto Mundim Porto ; Cota, Bianca Della Croce Vieira ; Silva-Filho, Agnaldo Lopes da</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c412t-4af9c9efc445581b563473b048806a95913f63dabbe52ff98cd9e55957098e23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Adult</topic><topic>Aged</topic><topic>Carcinoma, Ovarian Epithelial</topic><topic>Embryology</topic><topic>Female</topic><topic>Humans</topic><topic>Immunity, Cellular</topic><topic>Interleukin-1 - biosynthesis</topic><topic>Interleukin-17 - biosynthesis</topic><topic>Interleukin-2 - biosynthesis</topic><topic>Medicine & Public Health</topic><topic>Middle Aged</topic><topic>Neoplasms, Glandular and Epithelial - diagnosis</topic><topic>Neoplasms, Glandular and Epithelial - immunology</topic><topic>Neoplasms, Glandular and Epithelial - mortality</topic><topic>Obstetrics/Perinatology/Midwifery</topic><topic>Original Article</topic><topic>Ovarian Neoplasms - diagnosis</topic><topic>Ovarian Neoplasms - immunology</topic><topic>Ovarian Neoplasms - mortality</topic><topic>Prospective Studies</topic><topic>Reproductive Medicine</topic><topic>Survival Rate - trends</topic><topic>Th1 Cells - immunology</topic><topic>Th1 Cells - metabolism</topic><topic>Th1 Cells - pathology</topic><topic>Th17 Cells - immunology</topic><topic>Th17 Cells - metabolism</topic><topic>Th17 Cells - pathology</topic><topic>Th2 Cells - immunology</topic><topic>Th2 Cells - metabolism</topic><topic>Th2 Cells - pathology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Cândido, Eduardo Batista</creatorcontrib><creatorcontrib>Silva, Luciana Maria</creatorcontrib><creatorcontrib>Carvalho, Andréa Teixeira</creatorcontrib><creatorcontrib>Lamaita, Rívia Mara</creatorcontrib><creatorcontrib>Filho, Roberto Mundim Porto</creatorcontrib><creatorcontrib>Cota, Bianca Della Croce Vieira</creatorcontrib><creatorcontrib>Silva-Filho, Agnaldo Lopes da</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><jtitle>Reproductive sciences (Thousand Oaks, Calif.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Cândido, Eduardo Batista</au><au>Silva, Luciana Maria</au><au>Carvalho, Andréa Teixeira</au><au>Lamaita, Rívia Mara</au><au>Filho, Roberto Mundim Porto</au><au>Cota, Bianca Della Croce Vieira</au><au>Silva-Filho, Agnaldo Lopes da</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Immune Response Evaluation Through Determination of Type 1, Type 2, and Type 17 Patterns in Patients With Epithelial Ovarian Cancer</atitle><jtitle>Reproductive sciences (Thousand Oaks, Calif.)</jtitle><stitle>Reprod. Sci</stitle><addtitle>Reprod Sci</addtitle><date>2013-07-01</date><risdate>2013</risdate><volume>20</volume><issue>7</issue><spage>828</spage><epage>837</epage><pages>828-837</pages><issn>1933-7191</issn><eissn>1933-7205</eissn><abstract>Innate and adaptive immune cells secrete different cytokines, which participate through distinct mechanisms in cell-mediated immunity and humoral immune responses. The aim of this study was to evaluate the immune response through analysis of type 1 (Th1), Th2, and Th17 cells in patients with epithelial ovarian cancer (EOC). Our study included 44 patients with EOC (study group) and 32 gynecological patients with no ovarian disease (control group). Fragments of ovarian tissue and blood samples were collected in both groups and aliquots of intracystic fluid and peritoneal fluid were recovered from the EOC patient group. Interleukin (IL)-2/IL-4/IL-6/IL-10/IL-17/tumor necrosis factor (TNF)-α/interferon (IFN)-γ levels were measured by cytometric bead array. Statistical analysis included chi-squared, Student t, Mann-Whitney, Kruskal-Wallis tests, and Cox regression model. Patients with EOC were associated with higher levels of TNF-α/IL-4/IL-6/IL-10 compared to the control group. Both IL-10 and TNF-α concentrations were higher in patients with stage III/IV EOC and also associated with higher levels of cancer antigen 125. Higher Th1-mediated immune response was observed when the cytoreduction was considered optimal. However, patients with EOC with unsatisfactory cytoreductive surgery and undifferentiated tumors were associated with higher concentrations of Th2 cytokines in the 4 sites studied. Higher IL-6/IL-10 and lower IFN-γ concentrations were also associated with a lower overall survival rate in patients with EOC. The EOC group presented a predominantly Th2 response and an immunosuppressant standard and had association between IL-6/IL-10/IFN-γ and prognosis.</abstract><cop>Los Angeles, CA</cop><pub>SAGE Publications</pub><pmid>23239818</pmid><doi>10.1177/1933719112466299</doi><tpages>10</tpages></addata></record> |
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| identifier | ISSN: 1933-7191 |
| ispartof | Reproductive sciences (Thousand Oaks, Calif.), 2013-07, Vol.20 (7), p.828-837 |
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| subjects | Adult Aged Carcinoma, Ovarian Epithelial Embryology Female Humans Immunity, Cellular Interleukin-1 - biosynthesis Interleukin-17 - biosynthesis Interleukin-2 - biosynthesis Medicine & Public Health Middle Aged Neoplasms, Glandular and Epithelial - diagnosis Neoplasms, Glandular and Epithelial - immunology Neoplasms, Glandular and Epithelial - mortality Obstetrics/Perinatology/Midwifery Original Article Ovarian Neoplasms - diagnosis Ovarian Neoplasms - immunology Ovarian Neoplasms - mortality Prospective Studies Reproductive Medicine Survival Rate - trends Th1 Cells - immunology Th1 Cells - metabolism Th1 Cells - pathology Th17 Cells - immunology Th17 Cells - metabolism Th17 Cells - pathology Th2 Cells - immunology Th2 Cells - metabolism Th2 Cells - pathology |
| title | Immune Response Evaluation Through Determination of Type 1, Type 2, and Type 17 Patterns in Patients With Epithelial Ovarian Cancer |
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