The domain of unknown function DUF1521 exhibits metal ion-inducible autocleavage activity – a novel example from a putative effector protein of Vibrio coralliilyticus ATCC BAA-450
Abstract Vibrio coralliilyticus ATCC BAA-450 is a pathogen causing coral bleaching at elevated seawater temperatures. Based on the available genome sequence, the strain has a type III secretion system. Within the corresponding gene cluster, VIC_001052 is encoded, which contains a conserved domain of...
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| Veröffentlicht in: | FEMS microbiology letters 2013-06, Vol.343 (2), p.177-182 |
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| container_title | FEMS microbiology letters |
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| creator | Schirrmeister, Jana Zocher, Sara Flor, Liane Göttfert, Michael Zehner, Susanne |
| description | Abstract
Vibrio coralliilyticus ATCC BAA-450 is a pathogen causing coral bleaching at elevated seawater temperatures. Based on the available genome sequence, the strain has a type III secretion system. Within the corresponding gene cluster, VIC_001052 is encoded, which contains a conserved domain of unknown function DUF1521. In this study, we show that the purified domain exhibits autocleavage activity in the presence of several divalent metal ions, for example, calcium and manganese but not with magnesium or zinc. Autocleavage is not affected by temperatures between 0 and 30 °C, indicating that seawater temperature is not a critical factor for this activity. The DUF1521 domain and the cleavage site are conserved in several proteins from proteobacteria, suggesting a similar cleavage activity for these proteins. |
| doi_str_mv | 10.1111/1574-6968.12145 |
| format | Article |
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Vibrio coralliilyticus ATCC BAA-450 is a pathogen causing coral bleaching at elevated seawater temperatures. Based on the available genome sequence, the strain has a type III secretion system. Within the corresponding gene cluster, VIC_001052 is encoded, which contains a conserved domain of unknown function DUF1521. In this study, we show that the purified domain exhibits autocleavage activity in the presence of several divalent metal ions, for example, calcium and manganese but not with magnesium or zinc. Autocleavage is not affected by temperatures between 0 and 30 °C, indicating that seawater temperature is not a critical factor for this activity. The DUF1521 domain and the cleavage site are conserved in several proteins from proteobacteria, suggesting a similar cleavage activity for these proteins.</description><identifier>ISSN: 0378-1097</identifier><identifier>EISSN: 1574-6968</identifier><identifier>DOI: 10.1111/1574-6968.12145</identifier><identifier>PMID: 23551226</identifier><identifier>CODEN: FMLED7</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Amino Acid Motifs ; Amino Acid Sequence ; Bacterial Proteins - chemistry ; Bacterial Proteins - genetics ; Bacterial Proteins - isolation & purification ; Bacterial Proteins - metabolism ; Bacteriology ; Biological and medical sciences ; Conserved Sequence ; DUF1521 ; Fundamental and applied biological sciences. Psychology ; Gene Expression ; Ions - metabolism ; metal ion‐inducible autocleavage ; Microbiology ; Miscellaneous ; Multigene Family ; Protein Interaction Domains and Motifs ; Proteobacteria ; secreted effector ; Temperature ; Vibrio ; Vibrio - genetics ; Vibrio - metabolism</subject><ispartof>FEMS microbiology letters, 2013-06, Vol.343 (2), p.177-182</ispartof><rights>2013 Federation of European Microbiological Societies. Published by John Wiley & Sons Ltd. All rights reserved 2013</rights><rights>2013 Federation of European Microbiological Societies. Published by John Wiley & Sons Ltd. All rights reserved</rights><rights>2014 INIST-CNRS</rights><rights>2013 Federation of European Microbiological Societies. Published by John Wiley & Sons Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4415-f0272fcbd0c8136798f43ae9e51630f121b0c7cc3f5df5e5cd97d60961ba5cc03</citedby><cites>FETCH-LOGICAL-c4415-f0272fcbd0c8136798f43ae9e51630f121b0c7cc3f5df5e5cd97d60961ba5cc03</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2F1574-6968.12145$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2F1574-6968.12145$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27903,27904,45553,45554</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=27394513$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23551226$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Schirrmeister, Jana</creatorcontrib><creatorcontrib>Zocher, Sara</creatorcontrib><creatorcontrib>Flor, Liane</creatorcontrib><creatorcontrib>Göttfert, Michael</creatorcontrib><creatorcontrib>Zehner, Susanne</creatorcontrib><title>The domain of unknown function DUF1521 exhibits metal ion-inducible autocleavage activity – a novel example from a putative effector protein of Vibrio coralliilyticus ATCC BAA-450</title><title>FEMS microbiology letters</title><addtitle>FEMS Microbiol Lett</addtitle><description>Abstract
Vibrio coralliilyticus ATCC BAA-450 is a pathogen causing coral bleaching at elevated seawater temperatures. Based on the available genome sequence, the strain has a type III secretion system. Within the corresponding gene cluster, VIC_001052 is encoded, which contains a conserved domain of unknown function DUF1521. In this study, we show that the purified domain exhibits autocleavage activity in the presence of several divalent metal ions, for example, calcium and manganese but not with magnesium or zinc. Autocleavage is not affected by temperatures between 0 and 30 °C, indicating that seawater temperature is not a critical factor for this activity. The DUF1521 domain and the cleavage site are conserved in several proteins from proteobacteria, suggesting a similar cleavage activity for these proteins.</description><subject>Amino Acid Motifs</subject><subject>Amino Acid Sequence</subject><subject>Bacterial Proteins - chemistry</subject><subject>Bacterial Proteins - genetics</subject><subject>Bacterial Proteins - isolation & purification</subject><subject>Bacterial Proteins - metabolism</subject><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>Conserved Sequence</subject><subject>DUF1521</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression</subject><subject>Ions - metabolism</subject><subject>metal ion‐inducible autocleavage</subject><subject>Microbiology</subject><subject>Miscellaneous</subject><subject>Multigene Family</subject><subject>Protein Interaction Domains and Motifs</subject><subject>Proteobacteria</subject><subject>secreted effector</subject><subject>Temperature</subject><subject>Vibrio</subject><subject>Vibrio - genetics</subject><subject>Vibrio - metabolism</subject><issn>0378-1097</issn><issn>1574-6968</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkc1uEzEUhS0EoqGwZoe8QUJI09rjn4mXaSCAFMQmZTvyeGxq8IyDf1Ky4x14Fl6IJ8Fh0sICCbyxfP2de4_uAeAxRme4nHPMGlpxwednuMaU3QGz28pdMEOkmVcYieYEPIjxI0KI1ojfByc1YQzXNZ-B75srDXs_SDtCb2AeP43-eoQmjypZP8IXlyvMagz1lyvb2RThoJN0sHxVduyzsp3TUObkldNyJz-URxHubNrDH1-_QQlHv9OuyOWwLaQJfijFbU6yUBpqY7RKPsBt8ElPHt7bLlgPlQ_SOWvdPlmVI1xslkt4sVhUlKGH4J6RLupHx_sUXK5ebpavq_W7V2-Wi3WlKMWsMqhuaqO6Hqk5JrwRc0OJ1EIzzAkyZWUdUo1SxLDeMM1UL5qeI8FxJ5lSiJyCZ1PfYu9z1jG1g41KOydH7XNsMRFCIE4Z-w-UccGoYLyg5xOqgo8xaNNugx1k2LcYtYdY20OI7SHE9lesRfHk2Dx3g-5v-ZscC_D0CMiopDNBjsrG31xDBGWYFI5N3LV1ev-vue3q7frGwPNJ5_P2r6rqD7c_Afxzx4E</recordid><startdate>201306</startdate><enddate>201306</enddate><creator>Schirrmeister, Jana</creator><creator>Zocher, Sara</creator><creator>Flor, Liane</creator><creator>Göttfert, Michael</creator><creator>Zehner, Susanne</creator><general>Blackwell Publishing Ltd</general><general>Wiley-Blackwell</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QL</scope><scope>C1K</scope><scope>F1W</scope><scope>H95</scope><scope>H97</scope><scope>L.G</scope></search><sort><creationdate>201306</creationdate><title>The domain of unknown function DUF1521 exhibits metal ion-inducible autocleavage activity – a novel example from a putative effector protein of Vibrio coralliilyticus ATCC BAA-450</title><author>Schirrmeister, Jana ; Zocher, Sara ; Flor, Liane ; Göttfert, Michael ; Zehner, Susanne</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4415-f0272fcbd0c8136798f43ae9e51630f121b0c7cc3f5df5e5cd97d60961ba5cc03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Amino Acid Motifs</topic><topic>Amino Acid Sequence</topic><topic>Bacterial Proteins - chemistry</topic><topic>Bacterial Proteins - genetics</topic><topic>Bacterial Proteins - isolation & purification</topic><topic>Bacterial Proteins - metabolism</topic><topic>Bacteriology</topic><topic>Biological and medical sciences</topic><topic>Conserved Sequence</topic><topic>DUF1521</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression</topic><topic>Ions - metabolism</topic><topic>metal ion‐inducible autocleavage</topic><topic>Microbiology</topic><topic>Miscellaneous</topic><topic>Multigene Family</topic><topic>Protein Interaction Domains and Motifs</topic><topic>Proteobacteria</topic><topic>secreted effector</topic><topic>Temperature</topic><topic>Vibrio</topic><topic>Vibrio - genetics</topic><topic>Vibrio - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Schirrmeister, Jana</creatorcontrib><creatorcontrib>Zocher, Sara</creatorcontrib><creatorcontrib>Flor, Liane</creatorcontrib><creatorcontrib>Göttfert, Michael</creatorcontrib><creatorcontrib>Zehner, Susanne</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 3: Aquatic Pollution & Environmental Quality</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><jtitle>FEMS microbiology letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Schirrmeister, Jana</au><au>Zocher, Sara</au><au>Flor, Liane</au><au>Göttfert, Michael</au><au>Zehner, Susanne</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The domain of unknown function DUF1521 exhibits metal ion-inducible autocleavage activity – a novel example from a putative effector protein of Vibrio coralliilyticus ATCC BAA-450</atitle><jtitle>FEMS microbiology letters</jtitle><addtitle>FEMS Microbiol Lett</addtitle><date>2013-06</date><risdate>2013</risdate><volume>343</volume><issue>2</issue><spage>177</spage><epage>182</epage><pages>177-182</pages><issn>0378-1097</issn><eissn>1574-6968</eissn><coden>FMLED7</coden><abstract>Abstract
Vibrio coralliilyticus ATCC BAA-450 is a pathogen causing coral bleaching at elevated seawater temperatures. Based on the available genome sequence, the strain has a type III secretion system. Within the corresponding gene cluster, VIC_001052 is encoded, which contains a conserved domain of unknown function DUF1521. In this study, we show that the purified domain exhibits autocleavage activity in the presence of several divalent metal ions, for example, calcium and manganese but not with magnesium or zinc. Autocleavage is not affected by temperatures between 0 and 30 °C, indicating that seawater temperature is not a critical factor for this activity. The DUF1521 domain and the cleavage site are conserved in several proteins from proteobacteria, suggesting a similar cleavage activity for these proteins.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>23551226</pmid><doi>10.1111/1574-6968.12145</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0378-1097 |
| ispartof | FEMS microbiology letters, 2013-06, Vol.343 (2), p.177-182 |
| issn | 0378-1097 1574-6968 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1399906455 |
| source | MEDLINE; Wiley Online Library Journals Frontfile Complete; Oxford University Press Journals All Titles (1996-Current) |
| subjects | Amino Acid Motifs Amino Acid Sequence Bacterial Proteins - chemistry Bacterial Proteins - genetics Bacterial Proteins - isolation & purification Bacterial Proteins - metabolism Bacteriology Biological and medical sciences Conserved Sequence DUF1521 Fundamental and applied biological sciences. Psychology Gene Expression Ions - metabolism metal ion‐inducible autocleavage Microbiology Miscellaneous Multigene Family Protein Interaction Domains and Motifs Proteobacteria secreted effector Temperature Vibrio Vibrio - genetics Vibrio - metabolism |
| title | The domain of unknown function DUF1521 exhibits metal ion-inducible autocleavage activity – a novel example from a putative effector protein of Vibrio coralliilyticus ATCC BAA-450 |
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