Clinical isolates of Pseudomonas aeruginosa from superficial skin infections have different physiological patterns
Abstract Pseudomonas aeruginosa are known to have a wide physiological potential allowing them to constantly populate diverse environments leading to severe infections of humans such as septicemia, leg ulcers, and burn wounds. We set out to probe physiological characteristics of P. aeruginosa isolat...
Gespeichert in:
| Veröffentlicht in: | FEMS microbiology letters 2013-06, Vol.343 (2), p.183-189 |
|---|---|
| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 189 |
|---|---|
| container_issue | 2 |
| container_start_page | 183 |
| container_title | FEMS microbiology letters |
| container_volume | 343 |
| creator | Buivydas, Andrius Pasanen, Tanja Senčilo, Ana Daugelavičius, Rimantas Vaara, Martti Bamford, Dennis H. |
| description | Abstract
Pseudomonas aeruginosa are known to have a wide physiological potential allowing them to constantly populate diverse environments leading to severe infections of humans such as septicemia, leg ulcers, and burn wounds. We set out to probe physiological characteristics of P. aeruginosa isolates from diabetic leg ulcers collected from Helsinki metropolitan area. A total of 61 clinical isolates were obtained. Detailed phenotypic (physiological) characteristics [outer membrane (OM) permeability, membrane voltage, and activity of multidrug resistance pumps] were determined in several growth phases leading to the division of the analyzed set of P. aeruginosa strains into five distinct clusters including cells with similar physiological properties. In addition, their antibiotic resistance patterns and genetic heterogeneity were determined. Multiple isolates from the same patient were genetically very closely related and belonged to the same phenotypic cluster. However, genetically close isolates from different patients expressed very different phenotypic properties. The characteristics of infected patients seem to determine the growth environments for microorganisms that adapt by changing their physiological and/or genetic properties. |
| doi_str_mv | 10.1111/1574-6968.12148 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1399906452</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><oup_id>10.1111/1574-6968.12148</oup_id><sourcerecordid>3318464541</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4698-b09a665e5f19c3b9aff19ba86d0cb3ba8ed410328ad0c82f997add061a6a4d5d3</originalsourceid><addsrcrecordid>eNqNkV2L1DAUhoO4uOPqtXcSEEGE7iZtkjaXMrgqzKIXeh1O22Q3a5vUnFaZf29mZ_YDETQ3ORye93y9hLzg7JTnd8ZlLQqlVXPKSy6aR2R1l3lMVqyqm4IzXR-Tp4jXjDFRMvWEHJeV1ExWbEXSevDBdzBQj3GA2SKNjn5Bu_RxjAGQgk3LpQ8RgboUR4rLZJPznc8a_O4D9cHZbvYxIL2Cn5b23jmbbJjpdLVFH4d4edNggnm2KeAzcuRgQPv88J-Qb-fvv64_FpvPHz6t322KTijdFC3ToJS00nHdVa0Gl4MWGtWzrq1yYHvBWVU2kBNN6bSuoe-Z4qBA9LKvTsibfd0pxR-LxdmMHjs7DBBsXNDwSmvNlJDlf6BSaSl0LTL66g_0Oi4p5EUMzxflSjSSZepsT3UpIibrzJT8CGlrODM758zOJ7Pzydw4lxUvD3WXdrT9HX9rVQZeHwDAfE6XIHQe77m60kLy3YByz_3yg93-q685v9jcDvB2r4vL9FdV8WDa30V8vZw</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1530164850</pqid></control><display><type>article</type><title>Clinical isolates of Pseudomonas aeruginosa from superficial skin infections have different physiological patterns</title><source>Oxford University Press Journals All Titles (1996-Current)</source><source>MEDLINE</source><source>Wiley Online Library Journals Frontfile Complete</source><creator>Buivydas, Andrius ; Pasanen, Tanja ; Senčilo, Ana ; Daugelavičius, Rimantas ; Vaara, Martti ; Bamford, Dennis H.</creator><creatorcontrib>Buivydas, Andrius ; Pasanen, Tanja ; Senčilo, Ana ; Daugelavičius, Rimantas ; Vaara, Martti ; Bamford, Dennis H.</creatorcontrib><description>Abstract
Pseudomonas aeruginosa are known to have a wide physiological potential allowing them to constantly populate diverse environments leading to severe infections of humans such as septicemia, leg ulcers, and burn wounds. We set out to probe physiological characteristics of P. aeruginosa isolates from diabetic leg ulcers collected from Helsinki metropolitan area. A total of 61 clinical isolates were obtained. Detailed phenotypic (physiological) characteristics [outer membrane (OM) permeability, membrane voltage, and activity of multidrug resistance pumps] were determined in several growth phases leading to the division of the analyzed set of P. aeruginosa strains into five distinct clusters including cells with similar physiological properties. In addition, their antibiotic resistance patterns and genetic heterogeneity were determined. Multiple isolates from the same patient were genetically very closely related and belonged to the same phenotypic cluster. However, genetically close isolates from different patients expressed very different phenotypic properties. The characteristics of infected patients seem to determine the growth environments for microorganisms that adapt by changing their physiological and/or genetic properties.</description><identifier>ISSN: 0378-1097</identifier><identifier>EISSN: 1574-6968</identifier><identifier>DOI: 10.1111/1574-6968.12148</identifier><identifier>PMID: 23590530</identifier><identifier>CODEN: FMLED7</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Antibiotic resistance ; Bacteriology ; Biological and medical sciences ; Drug Resistance, Bacterial ; efflux pumps ; Fundamental and applied biological sciences. Psychology ; Genetic Variation ; Genotype ; Heterogeneity ; Humans ; Metropolitan areas ; microbial physiology ; Microbial Sensitivity Tests ; Microbiology ; Microorganisms ; Miscellaneous ; Onium Compounds - metabolism ; Organophosphorus Compounds - metabolism ; Phenotype ; Phylogeny ; Physiology ; Pseudomonas aeruginosa ; Pseudomonas aeruginosa - classification ; Pseudomonas aeruginosa - drug effects ; Pseudomonas aeruginosa - isolation & purification ; Pseudomonas aeruginosa - physiology ; Pseudomonas Infections - microbiology ; Skin Diseases, Infectious - microbiology ; tetraphenylphosphonium</subject><ispartof>FEMS microbiology letters, 2013-06, Vol.343 (2), p.183-189</ispartof><rights>2013 Federation of European Microbiological Societies. Published by John Wiley & Sons Ltd. All rights reserved 2013</rights><rights>2013 Federation of European Microbiological Societies. Published by John Wiley & Sons Ltd. All rights reserved</rights><rights>2014 INIST-CNRS</rights><rights>2013 Federation of European Microbiological Societies. Published by John Wiley & Sons Ltd. All rights reserved.</rights><rights>Copyright © 2013 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4698-b09a665e5f19c3b9aff19ba86d0cb3ba8ed410328ad0c82f997add061a6a4d5d3</citedby><cites>FETCH-LOGICAL-c4698-b09a665e5f19c3b9aff19ba86d0cb3ba8ed410328ad0c82f997add061a6a4d5d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2F1574-6968.12148$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2F1574-6968.12148$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27903,27904,45553,45554</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=27394514$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23590530$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Buivydas, Andrius</creatorcontrib><creatorcontrib>Pasanen, Tanja</creatorcontrib><creatorcontrib>Senčilo, Ana</creatorcontrib><creatorcontrib>Daugelavičius, Rimantas</creatorcontrib><creatorcontrib>Vaara, Martti</creatorcontrib><creatorcontrib>Bamford, Dennis H.</creatorcontrib><title>Clinical isolates of Pseudomonas aeruginosa from superficial skin infections have different physiological patterns</title><title>FEMS microbiology letters</title><addtitle>FEMS Microbiol Lett</addtitle><description>Abstract
Pseudomonas aeruginosa are known to have a wide physiological potential allowing them to constantly populate diverse environments leading to severe infections of humans such as septicemia, leg ulcers, and burn wounds. We set out to probe physiological characteristics of P. aeruginosa isolates from diabetic leg ulcers collected from Helsinki metropolitan area. A total of 61 clinical isolates were obtained. Detailed phenotypic (physiological) characteristics [outer membrane (OM) permeability, membrane voltage, and activity of multidrug resistance pumps] were determined in several growth phases leading to the division of the analyzed set of P. aeruginosa strains into five distinct clusters including cells with similar physiological properties. In addition, their antibiotic resistance patterns and genetic heterogeneity were determined. Multiple isolates from the same patient were genetically very closely related and belonged to the same phenotypic cluster. However, genetically close isolates from different patients expressed very different phenotypic properties. The characteristics of infected patients seem to determine the growth environments for microorganisms that adapt by changing their physiological and/or genetic properties.</description><subject>Antibiotic resistance</subject><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>Drug Resistance, Bacterial</subject><subject>efflux pumps</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genetic Variation</subject><subject>Genotype</subject><subject>Heterogeneity</subject><subject>Humans</subject><subject>Metropolitan areas</subject><subject>microbial physiology</subject><subject>Microbial Sensitivity Tests</subject><subject>Microbiology</subject><subject>Microorganisms</subject><subject>Miscellaneous</subject><subject>Onium Compounds - metabolism</subject><subject>Organophosphorus Compounds - metabolism</subject><subject>Phenotype</subject><subject>Phylogeny</subject><subject>Physiology</subject><subject>Pseudomonas aeruginosa</subject><subject>Pseudomonas aeruginosa - classification</subject><subject>Pseudomonas aeruginosa - drug effects</subject><subject>Pseudomonas aeruginosa - isolation & purification</subject><subject>Pseudomonas aeruginosa - physiology</subject><subject>Pseudomonas Infections - microbiology</subject><subject>Skin Diseases, Infectious - microbiology</subject><subject>tetraphenylphosphonium</subject><issn>0378-1097</issn><issn>1574-6968</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkV2L1DAUhoO4uOPqtXcSEEGE7iZtkjaXMrgqzKIXeh1O22Q3a5vUnFaZf29mZ_YDETQ3ORye93y9hLzg7JTnd8ZlLQqlVXPKSy6aR2R1l3lMVqyqm4IzXR-Tp4jXjDFRMvWEHJeV1ExWbEXSevDBdzBQj3GA2SKNjn5Bu_RxjAGQgk3LpQ8RgboUR4rLZJPznc8a_O4D9cHZbvYxIL2Cn5b23jmbbJjpdLVFH4d4edNggnm2KeAzcuRgQPv88J-Qb-fvv64_FpvPHz6t322KTijdFC3ToJS00nHdVa0Gl4MWGtWzrq1yYHvBWVU2kBNN6bSuoe-Z4qBA9LKvTsibfd0pxR-LxdmMHjs7DBBsXNDwSmvNlJDlf6BSaSl0LTL66g_0Oi4p5EUMzxflSjSSZepsT3UpIibrzJT8CGlrODM758zOJ7Pzydw4lxUvD3WXdrT9HX9rVQZeHwDAfE6XIHQe77m60kLy3YByz_3yg93-q685v9jcDvB2r4vL9FdV8WDa30V8vZw</recordid><startdate>201306</startdate><enddate>201306</enddate><creator>Buivydas, Andrius</creator><creator>Pasanen, Tanja</creator><creator>Senčilo, Ana</creator><creator>Daugelavičius, Rimantas</creator><creator>Vaara, Martti</creator><creator>Bamford, Dennis H.</creator><general>Blackwell Publishing Ltd</general><general>Wiley-Blackwell</general><general>Oxford University Press</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7T7</scope><scope>7TK</scope><scope>7TM</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>K9.</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>201306</creationdate><title>Clinical isolates of Pseudomonas aeruginosa from superficial skin infections have different physiological patterns</title><author>Buivydas, Andrius ; Pasanen, Tanja ; Senčilo, Ana ; Daugelavičius, Rimantas ; Vaara, Martti ; Bamford, Dennis H.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4698-b09a665e5f19c3b9aff19ba86d0cb3ba8ed410328ad0c82f997add061a6a4d5d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Antibiotic resistance</topic><topic>Bacteriology</topic><topic>Biological and medical sciences</topic><topic>Drug Resistance, Bacterial</topic><topic>efflux pumps</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genetic Variation</topic><topic>Genotype</topic><topic>Heterogeneity</topic><topic>Humans</topic><topic>Metropolitan areas</topic><topic>microbial physiology</topic><topic>Microbial Sensitivity Tests</topic><topic>Microbiology</topic><topic>Microorganisms</topic><topic>Miscellaneous</topic><topic>Onium Compounds - metabolism</topic><topic>Organophosphorus Compounds - metabolism</topic><topic>Phenotype</topic><topic>Phylogeny</topic><topic>Physiology</topic><topic>Pseudomonas aeruginosa</topic><topic>Pseudomonas aeruginosa - classification</topic><topic>Pseudomonas aeruginosa - drug effects</topic><topic>Pseudomonas aeruginosa - isolation & purification</topic><topic>Pseudomonas aeruginosa - physiology</topic><topic>Pseudomonas Infections - microbiology</topic><topic>Skin Diseases, Infectious - microbiology</topic><topic>tetraphenylphosphonium</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Buivydas, Andrius</creatorcontrib><creatorcontrib>Pasanen, Tanja</creatorcontrib><creatorcontrib>Senčilo, Ana</creatorcontrib><creatorcontrib>Daugelavičius, Rimantas</creatorcontrib><creatorcontrib>Vaara, Martti</creatorcontrib><creatorcontrib>Bamford, Dennis H.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>FEMS microbiology letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Buivydas, Andrius</au><au>Pasanen, Tanja</au><au>Senčilo, Ana</au><au>Daugelavičius, Rimantas</au><au>Vaara, Martti</au><au>Bamford, Dennis H.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Clinical isolates of Pseudomonas aeruginosa from superficial skin infections have different physiological patterns</atitle><jtitle>FEMS microbiology letters</jtitle><addtitle>FEMS Microbiol Lett</addtitle><date>2013-06</date><risdate>2013</risdate><volume>343</volume><issue>2</issue><spage>183</spage><epage>189</epage><pages>183-189</pages><issn>0378-1097</issn><eissn>1574-6968</eissn><coden>FMLED7</coden><abstract>Abstract
Pseudomonas aeruginosa are known to have a wide physiological potential allowing them to constantly populate diverse environments leading to severe infections of humans such as septicemia, leg ulcers, and burn wounds. We set out to probe physiological characteristics of P. aeruginosa isolates from diabetic leg ulcers collected from Helsinki metropolitan area. A total of 61 clinical isolates were obtained. Detailed phenotypic (physiological) characteristics [outer membrane (OM) permeability, membrane voltage, and activity of multidrug resistance pumps] were determined in several growth phases leading to the division of the analyzed set of P. aeruginosa strains into five distinct clusters including cells with similar physiological properties. In addition, their antibiotic resistance patterns and genetic heterogeneity were determined. Multiple isolates from the same patient were genetically very closely related and belonged to the same phenotypic cluster. However, genetically close isolates from different patients expressed very different phenotypic properties. The characteristics of infected patients seem to determine the growth environments for microorganisms that adapt by changing their physiological and/or genetic properties.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>23590530</pmid><doi>10.1111/1574-6968.12148</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0378-1097 |
| ispartof | FEMS microbiology letters, 2013-06, Vol.343 (2), p.183-189 |
| issn | 0378-1097 1574-6968 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1399906452 |
| source | Oxford University Press Journals All Titles (1996-Current); MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Antibiotic resistance Bacteriology Biological and medical sciences Drug Resistance, Bacterial efflux pumps Fundamental and applied biological sciences. Psychology Genetic Variation Genotype Heterogeneity Humans Metropolitan areas microbial physiology Microbial Sensitivity Tests Microbiology Microorganisms Miscellaneous Onium Compounds - metabolism Organophosphorus Compounds - metabolism Phenotype Phylogeny Physiology Pseudomonas aeruginosa Pseudomonas aeruginosa - classification Pseudomonas aeruginosa - drug effects Pseudomonas aeruginosa - isolation & purification Pseudomonas aeruginosa - physiology Pseudomonas Infections - microbiology Skin Diseases, Infectious - microbiology tetraphenylphosphonium |
| title | Clinical isolates of Pseudomonas aeruginosa from superficial skin infections have different physiological patterns |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-27T18%3A47%3A25IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Clinical%20isolates%20of%20Pseudomonas%20aeruginosa%20from%20superficial%20skin%20infections%20have%20different%20physiological%20patterns&rft.jtitle=FEMS%20microbiology%20letters&rft.au=Buivydas,%20Andrius&rft.date=2013-06&rft.volume=343&rft.issue=2&rft.spage=183&rft.epage=189&rft.pages=183-189&rft.issn=0378-1097&rft.eissn=1574-6968&rft.coden=FMLED7&rft_id=info:doi/10.1111/1574-6968.12148&rft_dat=%3Cproquest_cross%3E3318464541%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1530164850&rft_id=info:pmid/23590530&rft_oup_id=10.1111/1574-6968.12148&rfr_iscdi=true |