Identification and characterization of superoxide dismutase in Phytophthora cinnamomi
Superoxide dismutase (SOD) activities of the oomycete Phytophthora cinnamomi were examined. Five polypeptides with manganese superoxide dismutase (MnSOD) activity were found in mycelium growing in liquid culture with relative molecular weights ranging from approximately 25 to 100 kDa. Comparison wit...
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| Veröffentlicht in: | Protoplasma 2013-06, Vol.250 (3), p.779-785 |
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| creator | Guzmán-Deara, Jerónimo Reyes-De la Cruz, Homero Beltrán-Peña, Elda María Castro-Mercado, Elda García-Pineda, Ernesto |
| description | Superoxide dismutase (SOD) activities of the oomycete
Phytophthora cinnamomi
were examined. Five polypeptides with manganese superoxide dismutase (MnSOD) activity were found in mycelium growing in liquid culture with relative molecular weights ranging from approximately 25 to 100 kDa. Comparison with characterized avocado SODs showed no evidence for the presence of either iron or copper/zinc SODs in
P
.
cinnamomi
. The level of activity of the MnSOD polypeptides decreased in the presence of avocado root or cell wall components. Growth of
P
.
cinnamomi
, measured as dry weight, increased when the mycelium was grown in the presence of superoxide anion (O
2
−
), which was added exogenously. Our results suggest that the metabolism of O
2
−
has an important role in the development of
P
.
cinnamomi
. |
| doi_str_mv | 10.1007/s00709-012-0464-9 |
| format | Article |
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Phytophthora cinnamomi
were examined. Five polypeptides with manganese superoxide dismutase (MnSOD) activity were found in mycelium growing in liquid culture with relative molecular weights ranging from approximately 25 to 100 kDa. Comparison with characterized avocado SODs showed no evidence for the presence of either iron or copper/zinc SODs in
P
.
cinnamomi
. The level of activity of the MnSOD polypeptides decreased in the presence of avocado root or cell wall components. Growth of
P
.
cinnamomi
, measured as dry weight, increased when the mycelium was grown in the presence of superoxide anion (O
2
−
), which was added exogenously. Our results suggest that the metabolism of O
2
−
has an important role in the development of
P
.
cinnamomi
.</description><identifier>ISSN: 0033-183X</identifier><identifier>EISSN: 1615-6102</identifier><identifier>DOI: 10.1007/s00709-012-0464-9</identifier><identifier>PMID: 23086260</identifier><language>eng</language><publisher>Vienna: Springer Vienna</publisher><subject>Biomedical and Life Sciences ; Cell Biology ; Cell Wall - chemistry ; Fungal Proteins - antagonists & inhibitors ; Fungal Proteins - chemistry ; Fungal Proteins - metabolism ; Hydrogen Peroxide - chemistry ; Isoenzymes - antagonists & inhibitors ; Isoenzymes - chemistry ; Isoenzymes - metabolism ; Life Sciences ; Mycelium - drug effects ; Mycelium - enzymology ; Mycelium - growth & development ; Oomycetes ; Original Article ; Oxidants - pharmacology ; Persea - microbiology ; Persea americana ; Phytophthora - drug effects ; Phytophthora - enzymology ; Phytophthora - growth & development ; Phytophthora cinnamomi ; Plant Roots - microbiology ; Plant Sciences ; Potassium Cyanide - chemistry ; Superoxide Dismutase - antagonists & inhibitors ; Superoxide Dismutase - chemistry ; Superoxide Dismutase - metabolism ; Superoxides - pharmacology ; Zoology</subject><ispartof>Protoplasma, 2013-06, Vol.250 (3), p.779-785</ispartof><rights>Springer-Verlag Wien 2012</rights><rights>Springer-Verlag Wien 2013</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c405t-3c2a35ee58077c2ba71370811dd154067d5bf2a1ebe2514fb57c805baeda694c3</citedby><cites>FETCH-LOGICAL-c405t-3c2a35ee58077c2ba71370811dd154067d5bf2a1ebe2514fb57c805baeda694c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00709-012-0464-9$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00709-012-0464-9$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23086260$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Guzmán-Deara, Jerónimo</creatorcontrib><creatorcontrib>Reyes-De la Cruz, Homero</creatorcontrib><creatorcontrib>Beltrán-Peña, Elda María</creatorcontrib><creatorcontrib>Castro-Mercado, Elda</creatorcontrib><creatorcontrib>García-Pineda, Ernesto</creatorcontrib><title>Identification and characterization of superoxide dismutase in Phytophthora cinnamomi</title><title>Protoplasma</title><addtitle>Protoplasma</addtitle><addtitle>Protoplasma</addtitle><description>Superoxide dismutase (SOD) activities of the oomycete
Phytophthora cinnamomi
were examined. Five polypeptides with manganese superoxide dismutase (MnSOD) activity were found in mycelium growing in liquid culture with relative molecular weights ranging from approximately 25 to 100 kDa. Comparison with characterized avocado SODs showed no evidence for the presence of either iron or copper/zinc SODs in
P
.
cinnamomi
. The level of activity of the MnSOD polypeptides decreased in the presence of avocado root or cell wall components. Growth of
P
.
cinnamomi
, measured as dry weight, increased when the mycelium was grown in the presence of superoxide anion (O
2
−
), which was added exogenously. Our results suggest that the metabolism of O
2
−
has an important role in the development of
P
.
cinnamomi
.</description><subject>Biomedical and Life Sciences</subject><subject>Cell Biology</subject><subject>Cell Wall - chemistry</subject><subject>Fungal Proteins - antagonists & inhibitors</subject><subject>Fungal Proteins - chemistry</subject><subject>Fungal Proteins - metabolism</subject><subject>Hydrogen Peroxide - chemistry</subject><subject>Isoenzymes - antagonists & inhibitors</subject><subject>Isoenzymes - chemistry</subject><subject>Isoenzymes - metabolism</subject><subject>Life Sciences</subject><subject>Mycelium - drug effects</subject><subject>Mycelium - enzymology</subject><subject>Mycelium - growth & development</subject><subject>Oomycetes</subject><subject>Original Article</subject><subject>Oxidants - pharmacology</subject><subject>Persea - microbiology</subject><subject>Persea americana</subject><subject>Phytophthora - drug effects</subject><subject>Phytophthora - enzymology</subject><subject>Phytophthora - growth & development</subject><subject>Phytophthora cinnamomi</subject><subject>Plant Roots - microbiology</subject><subject>Plant Sciences</subject><subject>Potassium Cyanide - chemistry</subject><subject>Superoxide Dismutase - antagonists & inhibitors</subject><subject>Superoxide Dismutase - chemistry</subject><subject>Superoxide Dismutase - metabolism</subject><subject>Superoxides - pharmacology</subject><subject>Zoology</subject><issn>0033-183X</issn><issn>1615-6102</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp1kMFKHTEUhoNY9FZ9gG7KQDdupj1JJsnMskhrBUEXXnAXMsmZ3lzuJNdkBqpPb2RskYKbBJLv_8_hI-QTha8UQH3L5YCuBspqaGRTdwdkRSUVtaTADskKgPOatvz-mHzMeQsAgoE4IseMQyuZhBVZXzkMkx-8NZOPoTLBVXZjkrETJv-0PMahyvMeU_zjHVbO53GeTMbKh-p28zjF_WbaxGQq60MwYxz9KfkwmF3Gs9f7hKx__ri7-FVf31xeXXy_rm0DYqq5ZYYLRNGCUpb1RlGuoKXUOSoakMqJfmCGYo9M0GbohbItiN6gM7JrLD8h50vvPsWHGfOkR58t7nYmYJyzprzrOmhU2xb0y3_oNs4plO0KJTiTrZKiUHShbIo5Jxz0PvnRpEdNQb8414tzXZzrF-e6K5nPr81zP6L7l_gruQBsAXL5Cr8xvRn9buszomOMzQ</recordid><startdate>20130601</startdate><enddate>20130601</enddate><creator>Guzmán-Deara, Jerónimo</creator><creator>Reyes-De la Cruz, Homero</creator><creator>Beltrán-Peña, Elda María</creator><creator>Castro-Mercado, Elda</creator><creator>García-Pineda, Ernesto</creator><general>Springer Vienna</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7RV</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>88G</scope><scope>8AO</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB0</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2M</scope><scope>M7P</scope><scope>NAPCQ</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PSYQQ</scope><scope>Q9U</scope><scope>M7N</scope></search><sort><creationdate>20130601</creationdate><title>Identification and characterization of superoxide dismutase in Phytophthora cinnamomi</title><author>Guzmán-Deara, Jerónimo ; Reyes-De la Cruz, Homero ; Beltrán-Peña, Elda María ; Castro-Mercado, Elda ; García-Pineda, Ernesto</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c405t-3c2a35ee58077c2ba71370811dd154067d5bf2a1ebe2514fb57c805baeda694c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Biomedical and Life Sciences</topic><topic>Cell Biology</topic><topic>Cell Wall - chemistry</topic><topic>Fungal Proteins - antagonists & inhibitors</topic><topic>Fungal Proteins - chemistry</topic><topic>Fungal Proteins - metabolism</topic><topic>Hydrogen Peroxide - chemistry</topic><topic>Isoenzymes - antagonists & inhibitors</topic><topic>Isoenzymes - chemistry</topic><topic>Isoenzymes - metabolism</topic><topic>Life Sciences</topic><topic>Mycelium - drug effects</topic><topic>Mycelium - enzymology</topic><topic>Mycelium - growth & development</topic><topic>Oomycetes</topic><topic>Original Article</topic><topic>Oxidants - pharmacology</topic><topic>Persea - microbiology</topic><topic>Persea americana</topic><topic>Phytophthora - drug effects</topic><topic>Phytophthora - enzymology</topic><topic>Phytophthora - growth & development</topic><topic>Phytophthora cinnamomi</topic><topic>Plant Roots - microbiology</topic><topic>Plant Sciences</topic><topic>Potassium Cyanide - chemistry</topic><topic>Superoxide Dismutase - antagonists & inhibitors</topic><topic>Superoxide Dismutase - chemistry</topic><topic>Superoxide Dismutase - metabolism</topic><topic>Superoxides - pharmacology</topic><topic>Zoology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Guzmán-Deara, Jerónimo</creatorcontrib><creatorcontrib>Reyes-De la Cruz, Homero</creatorcontrib><creatorcontrib>Beltrán-Peña, Elda María</creatorcontrib><creatorcontrib>Castro-Mercado, Elda</creatorcontrib><creatorcontrib>García-Pineda, Ernesto</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Proquest Nursing & Allied Health Source</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Psychology Database (Alumni)</collection><collection>ProQuest Pharma Collection</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Psychology Database</collection><collection>Biological Science Database</collection><collection>Nursing & Allied Health Premium</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest One Psychology</collection><collection>ProQuest Central Basic</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><jtitle>Protoplasma</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Guzmán-Deara, Jerónimo</au><au>Reyes-De la Cruz, Homero</au><au>Beltrán-Peña, Elda María</au><au>Castro-Mercado, Elda</au><au>García-Pineda, Ernesto</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Identification and characterization of superoxide dismutase in Phytophthora cinnamomi</atitle><jtitle>Protoplasma</jtitle><stitle>Protoplasma</stitle><addtitle>Protoplasma</addtitle><date>2013-06-01</date><risdate>2013</risdate><volume>250</volume><issue>3</issue><spage>779</spage><epage>785</epage><pages>779-785</pages><issn>0033-183X</issn><eissn>1615-6102</eissn><abstract>Superoxide dismutase (SOD) activities of the oomycete
Phytophthora cinnamomi
were examined. Five polypeptides with manganese superoxide dismutase (MnSOD) activity were found in mycelium growing in liquid culture with relative molecular weights ranging from approximately 25 to 100 kDa. Comparison with characterized avocado SODs showed no evidence for the presence of either iron or copper/zinc SODs in
P
.
cinnamomi
. The level of activity of the MnSOD polypeptides decreased in the presence of avocado root or cell wall components. Growth of
P
.
cinnamomi
, measured as dry weight, increased when the mycelium was grown in the presence of superoxide anion (O
2
−
), which was added exogenously. Our results suggest that the metabolism of O
2
−
has an important role in the development of
P
.
cinnamomi
.</abstract><cop>Vienna</cop><pub>Springer Vienna</pub><pmid>23086260</pmid><doi>10.1007/s00709-012-0464-9</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | Protoplasma, 2013-06, Vol.250 (3), p.779-785 |
| issn | 0033-183X 1615-6102 |
| language | eng |
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| source | MEDLINE; SpringerNature Journals |
| subjects | Biomedical and Life Sciences Cell Biology Cell Wall - chemistry Fungal Proteins - antagonists & inhibitors Fungal Proteins - chemistry Fungal Proteins - metabolism Hydrogen Peroxide - chemistry Isoenzymes - antagonists & inhibitors Isoenzymes - chemistry Isoenzymes - metabolism Life Sciences Mycelium - drug effects Mycelium - enzymology Mycelium - growth & development Oomycetes Original Article Oxidants - pharmacology Persea - microbiology Persea americana Phytophthora - drug effects Phytophthora - enzymology Phytophthora - growth & development Phytophthora cinnamomi Plant Roots - microbiology Plant Sciences Potassium Cyanide - chemistry Superoxide Dismutase - antagonists & inhibitors Superoxide Dismutase - chemistry Superoxide Dismutase - metabolism Superoxides - pharmacology Zoology |
| title | Identification and characterization of superoxide dismutase in Phytophthora cinnamomi |
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