Pleomorphomonas diazotrophica sp. nov., an endophytic N-fixing bacterium isolated from root tissue of Jatropha curcas L
A novel aerobic, non-motile, pleomorphic, Gram-negative and nitrogen-fixing bacterial strain, designated R5-392T, was isolated from surface-sterilized root tissue of Jatropha curcas. The organism grew optimally at 30 °C in media containing 1 % (w/v) NaCl and at pH 6.0–8.0. The predominant ubiquinone...
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| Veröffentlicht in: | International journal of systematic and evolutionary microbiology 2013-07, Vol.63 (Pt 7), p.2477-2483 |
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| container_title | International journal of systematic and evolutionary microbiology |
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| creator | Madhaiyan, Munusamy Jin, Tan Yuan Roy, Joseph Jegan Kim, Soo-Jin Weon, Hang-Yeon Kwon, Soon-Wo Ji, Lianghui |
| description | A novel aerobic, non-motile, pleomorphic, Gram-negative and nitrogen-fixing bacterial strain, designated R5-392T, was isolated from surface-sterilized root tissue of Jatropha curcas. The organism grew optimally at 30 °C in media containing 1 % (w/v) NaCl and at pH 6.0–8.0. The predominant ubiquinone was Q-10 and the major fatty acids were C18 : 1ω7c/C18 : 1ω6c, C16 : 0 and C19 : 0 cyclo ω8c. The DNA G+C content was 63.2 mol%. Analysis of the 16S rRNA gene sequence suggested that strain R5-392T is affiliated with the order Rhizobiales within the class Alphaproteobacteria and is most closely related to Pleomorphomonas oryzae F-7T (98.8 % similarity) and Pleomorphomonas koreensis Y9T (98.3 % similarity). Analysis of partial nifH gene sequences also revealed a monophyletic lineage within the class Alphaproteobacteria, and strain R5-392T was most closely related to P. oryzae F-7T (98 %). Highest nitrogenase activity was detected in the presence of low-level organic nitrogen or in the presence of nitrogenase co-factors (Fe/Mo) in N-free media. Phenotypic and chemotaxonomic data suggest that strain R5-392T represents a novel species within the genus Pleomorphomonas, for which the name Pleomorphomonas diazotrophica sp. nov. is proposed. The type strain is R5-392T ( = KACC 16233T = DSM 25022T). |
| doi_str_mv | 10.1099/ijs.0.044461-0 |
| format | Article |
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The organism grew optimally at 30 °C in media containing 1 % (w/v) NaCl and at pH 6.0–8.0. The predominant ubiquinone was Q-10 and the major fatty acids were C18 : 1ω7c/C18 : 1ω6c, C16 : 0 and C19 : 0 cyclo ω8c. The DNA G+C content was 63.2 mol%. Analysis of the 16S rRNA gene sequence suggested that strain R5-392T is affiliated with the order Rhizobiales within the class Alphaproteobacteria and is most closely related to Pleomorphomonas oryzae F-7T (98.8 % similarity) and Pleomorphomonas koreensis Y9T (98.3 % similarity). Analysis of partial nifH gene sequences also revealed a monophyletic lineage within the class Alphaproteobacteria, and strain R5-392T was most closely related to P. oryzae F-7T (98 %). Highest nitrogenase activity was detected in the presence of low-level organic nitrogen or in the presence of nitrogenase co-factors (Fe/Mo) in N-free media. Phenotypic and chemotaxonomic data suggest that strain R5-392T represents a novel species within the genus Pleomorphomonas, for which the name Pleomorphomonas diazotrophica sp. nov. is proposed. The type strain is R5-392T ( = KACC 16233T = DSM 25022T).</description><identifier>ISSN: 1466-5034</identifier><identifier>ISSN: 1466-5026</identifier><identifier>EISSN: 1466-5034</identifier><identifier>DOI: 10.1099/ijs.0.044461-0</identifier><identifier>PMID: 23223815</identifier><language>eng</language><publisher>England: International Union of Microbiological Societies</publisher><subject>Alphaproteobacteria - classification ; Alphaproteobacteria - genetics ; Alphaproteobacteria - isolation & purification ; bacteria ; Bacterial Typing Techniques ; Base Composition ; chemotaxonomy ; DNA ; DNA, Bacterial - genetics ; endophytes ; fatty acids ; Fatty Acids - analysis ; iron ; Jatropha - microbiology ; Jatropha curcas ; Molecular Sequence Data ; molybdenum ; monophyly ; nitrogen ; Nitrogen Fixation ; nitrogenase ; Nitrogenase - analysis ; nucleotide sequences ; Phylogeny ; Plant Roots - microbiology ; Quinones - analysis ; Rhizobiales ; ribosomal RNA ; RNA, Ribosomal, 16S - genetics ; Sequence Analysis, DNA ; sodium chloride ; ubiquinones</subject><ispartof>International journal of systematic and evolutionary microbiology, 2013-07, Vol.63 (Pt 7), p.2477-2483</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c319t-295bd5bc34c788a3397ae2ed620d8d95524b0bb6263d838f46965862a53a87d43</citedby><cites>FETCH-LOGICAL-c319t-295bd5bc34c788a3397ae2ed620d8d95524b0bb6263d838f46965862a53a87d43</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,3746,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23223815$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Madhaiyan, Munusamy</creatorcontrib><creatorcontrib>Jin, Tan Yuan</creatorcontrib><creatorcontrib>Roy, Joseph Jegan</creatorcontrib><creatorcontrib>Kim, Soo-Jin</creatorcontrib><creatorcontrib>Weon, Hang-Yeon</creatorcontrib><creatorcontrib>Kwon, Soon-Wo</creatorcontrib><creatorcontrib>Ji, Lianghui</creatorcontrib><title>Pleomorphomonas diazotrophica sp. nov., an endophytic N-fixing bacterium isolated from root tissue of Jatropha curcas L</title><title>International journal of systematic and evolutionary microbiology</title><addtitle>Int J Syst Evol Microbiol</addtitle><description>A novel aerobic, non-motile, pleomorphic, Gram-negative and nitrogen-fixing bacterial strain, designated R5-392T, was isolated from surface-sterilized root tissue of Jatropha curcas. The organism grew optimally at 30 °C in media containing 1 % (w/v) NaCl and at pH 6.0–8.0. The predominant ubiquinone was Q-10 and the major fatty acids were C18 : 1ω7c/C18 : 1ω6c, C16 : 0 and C19 : 0 cyclo ω8c. The DNA G+C content was 63.2 mol%. Analysis of the 16S rRNA gene sequence suggested that strain R5-392T is affiliated with the order Rhizobiales within the class Alphaproteobacteria and is most closely related to Pleomorphomonas oryzae F-7T (98.8 % similarity) and Pleomorphomonas koreensis Y9T (98.3 % similarity). Analysis of partial nifH gene sequences also revealed a monophyletic lineage within the class Alphaproteobacteria, and strain R5-392T was most closely related to P. oryzae F-7T (98 %). Highest nitrogenase activity was detected in the presence of low-level organic nitrogen or in the presence of nitrogenase co-factors (Fe/Mo) in N-free media. Phenotypic and chemotaxonomic data suggest that strain R5-392T represents a novel species within the genus Pleomorphomonas, for which the name Pleomorphomonas diazotrophica sp. nov. is proposed. The type strain is R5-392T ( = KACC 16233T = DSM 25022T).</description><subject>Alphaproteobacteria - classification</subject><subject>Alphaproteobacteria - genetics</subject><subject>Alphaproteobacteria - isolation & purification</subject><subject>bacteria</subject><subject>Bacterial Typing Techniques</subject><subject>Base Composition</subject><subject>chemotaxonomy</subject><subject>DNA</subject><subject>DNA, Bacterial - genetics</subject><subject>endophytes</subject><subject>fatty acids</subject><subject>Fatty Acids - analysis</subject><subject>iron</subject><subject>Jatropha - microbiology</subject><subject>Jatropha curcas</subject><subject>Molecular Sequence Data</subject><subject>molybdenum</subject><subject>monophyly</subject><subject>nitrogen</subject><subject>Nitrogen Fixation</subject><subject>nitrogenase</subject><subject>Nitrogenase - analysis</subject><subject>nucleotide sequences</subject><subject>Phylogeny</subject><subject>Plant Roots - microbiology</subject><subject>Quinones - analysis</subject><subject>Rhizobiales</subject><subject>ribosomal RNA</subject><subject>RNA, Ribosomal, 16S - genetics</subject><subject>Sequence Analysis, DNA</subject><subject>sodium chloride</subject><subject>ubiquinones</subject><issn>1466-5034</issn><issn>1466-5026</issn><issn>1466-5034</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpNkUtv1TAQhS0Eog_YsgQvWZAwfl57iapCQVeABF1bE9tpXSVxsBOg_HpSbkFsZkZH35yRzhDyjEHLwNrX6aa20IKUUrMGHpBjJrVuFAj58L_5iJzUegOwCQCPyREXnAvD1DH58XmIecxlvt7qhJWGhL_yUvJ8nTzSOrd0yt_bVxQnGqewybdL8vRj06efabqiHfollrSONNU84BID7Useacl5oUuqdY009_QD_rFE6tfityv7J-RRj0ONT-_7Kbl8e_717KLZf3r3_uzNvvGC2aXhVnVBdV5IvzMGhbA7jDwGzSGYYJXisoOu01yLYITppbZaGc1RCTS7IMUpeXnwnUv-tsa6uDFVH4cBp5jX6piwFpQGu9vQ9oD6kmstsXdzSSOWW8fA3YXttrAduEPYDraF5_feazfG8A__m-4GvDgAPWaHVyVVd_mFA1N3n5DAjfgNP5GEog</recordid><startdate>20130701</startdate><enddate>20130701</enddate><creator>Madhaiyan, Munusamy</creator><creator>Jin, Tan Yuan</creator><creator>Roy, Joseph Jegan</creator><creator>Kim, Soo-Jin</creator><creator>Weon, Hang-Yeon</creator><creator>Kwon, Soon-Wo</creator><creator>Ji, Lianghui</creator><general>International Union of Microbiological Societies</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20130701</creationdate><title>Pleomorphomonas diazotrophica sp. nov., an endophytic N-fixing bacterium isolated from root tissue of Jatropha curcas L</title><author>Madhaiyan, Munusamy ; Jin, Tan Yuan ; Roy, Joseph Jegan ; Kim, Soo-Jin ; Weon, Hang-Yeon ; Kwon, Soon-Wo ; Ji, Lianghui</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c319t-295bd5bc34c788a3397ae2ed620d8d95524b0bb6263d838f46965862a53a87d43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Alphaproteobacteria - classification</topic><topic>Alphaproteobacteria - genetics</topic><topic>Alphaproteobacteria - isolation & purification</topic><topic>bacteria</topic><topic>Bacterial Typing Techniques</topic><topic>Base Composition</topic><topic>chemotaxonomy</topic><topic>DNA</topic><topic>DNA, Bacterial - genetics</topic><topic>endophytes</topic><topic>fatty acids</topic><topic>Fatty Acids - analysis</topic><topic>iron</topic><topic>Jatropha - microbiology</topic><topic>Jatropha curcas</topic><topic>Molecular Sequence Data</topic><topic>molybdenum</topic><topic>monophyly</topic><topic>nitrogen</topic><topic>Nitrogen Fixation</topic><topic>nitrogenase</topic><topic>Nitrogenase - analysis</topic><topic>nucleotide sequences</topic><topic>Phylogeny</topic><topic>Plant Roots - microbiology</topic><topic>Quinones - analysis</topic><topic>Rhizobiales</topic><topic>ribosomal RNA</topic><topic>RNA, Ribosomal, 16S - genetics</topic><topic>Sequence Analysis, DNA</topic><topic>sodium chloride</topic><topic>ubiquinones</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Madhaiyan, Munusamy</creatorcontrib><creatorcontrib>Jin, Tan Yuan</creatorcontrib><creatorcontrib>Roy, Joseph Jegan</creatorcontrib><creatorcontrib>Kim, Soo-Jin</creatorcontrib><creatorcontrib>Weon, Hang-Yeon</creatorcontrib><creatorcontrib>Kwon, Soon-Wo</creatorcontrib><creatorcontrib>Ji, Lianghui</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>International journal of systematic and evolutionary microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Madhaiyan, Munusamy</au><au>Jin, Tan Yuan</au><au>Roy, Joseph Jegan</au><au>Kim, Soo-Jin</au><au>Weon, Hang-Yeon</au><au>Kwon, Soon-Wo</au><au>Ji, Lianghui</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Pleomorphomonas diazotrophica sp. nov., an endophytic N-fixing bacterium isolated from root tissue of Jatropha curcas L</atitle><jtitle>International journal of systematic and evolutionary microbiology</jtitle><addtitle>Int J Syst Evol Microbiol</addtitle><date>2013-07-01</date><risdate>2013</risdate><volume>63</volume><issue>Pt 7</issue><spage>2477</spage><epage>2483</epage><pages>2477-2483</pages><issn>1466-5034</issn><issn>1466-5026</issn><eissn>1466-5034</eissn><abstract>A novel aerobic, non-motile, pleomorphic, Gram-negative and nitrogen-fixing bacterial strain, designated R5-392T, was isolated from surface-sterilized root tissue of Jatropha curcas. The organism grew optimally at 30 °C in media containing 1 % (w/v) NaCl and at pH 6.0–8.0. The predominant ubiquinone was Q-10 and the major fatty acids were C18 : 1ω7c/C18 : 1ω6c, C16 : 0 and C19 : 0 cyclo ω8c. The DNA G+C content was 63.2 mol%. Analysis of the 16S rRNA gene sequence suggested that strain R5-392T is affiliated with the order Rhizobiales within the class Alphaproteobacteria and is most closely related to Pleomorphomonas oryzae F-7T (98.8 % similarity) and Pleomorphomonas koreensis Y9T (98.3 % similarity). Analysis of partial nifH gene sequences also revealed a monophyletic lineage within the class Alphaproteobacteria, and strain R5-392T was most closely related to P. oryzae F-7T (98 %). Highest nitrogenase activity was detected in the presence of low-level organic nitrogen or in the presence of nitrogenase co-factors (Fe/Mo) in N-free media. Phenotypic and chemotaxonomic data suggest that strain R5-392T represents a novel species within the genus Pleomorphomonas, for which the name Pleomorphomonas diazotrophica sp. nov. is proposed. The type strain is R5-392T ( = KACC 16233T = DSM 25022T).</abstract><cop>England</cop><pub>International Union of Microbiological Societies</pub><pmid>23223815</pmid><doi>10.1099/ijs.0.044461-0</doi><tpages>7</tpages></addata></record> |
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| ispartof | International journal of systematic and evolutionary microbiology, 2013-07, Vol.63 (Pt 7), p.2477-2483 |
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| source | MEDLINE; Microbiology Society; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | Alphaproteobacteria - classification Alphaproteobacteria - genetics Alphaproteobacteria - isolation & purification bacteria Bacterial Typing Techniques Base Composition chemotaxonomy DNA DNA, Bacterial - genetics endophytes fatty acids Fatty Acids - analysis iron Jatropha - microbiology Jatropha curcas Molecular Sequence Data molybdenum monophyly nitrogen Nitrogen Fixation nitrogenase Nitrogenase - analysis nucleotide sequences Phylogeny Plant Roots - microbiology Quinones - analysis Rhizobiales ribosomal RNA RNA, Ribosomal, 16S - genetics Sequence Analysis, DNA sodium chloride ubiquinones |
| title | Pleomorphomonas diazotrophica sp. nov., an endophytic N-fixing bacterium isolated from root tissue of Jatropha curcas L |
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