Functional brain GABA and glycine receptors in mRNA-injected Xenopus oocytes
Changes in membrane potential and conductance evoked by GABA were measured in Xenopus ooctyes injected with chick or rat brain mRNA. GABA responses were antagonized by bicuculline or picrotoxinin but enhanced by chlorazepate or by pentobarbitone. Oocytes injected with rat brain mRNA responded simila...
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| Veröffentlicht in: | Neuropharmacology 1984, Vol.23 (7), p.817-818 |
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| container_title | Neuropharmacology |
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| creator | Constanti, A. Houamed, K.M. Smart, T.G. Bilbe, G. Brown, D.A. Barnard, E.A. |
| description | Changes in membrane potential and conductance evoked by GABA were measured in Xenopus ooctyes injected with chick or rat brain mRNA. GABA responses were antagonized by bicuculline or picrotoxinin but enhanced by chlorazepate or by pentobarbitone. Oocytes injected with rat brain mRNA responded similarly to both GABA and glycine. Strychnine selectively depressed glycine responses, whereas picrotoxinin antagonized both GABA and glycine effects non-specifically. GABA (but not glycine) responses were enhanced by pentobarbitone. The cells also responded dose-dependently to glutamate, aspartate and kainate, indicating that this mRNA fraction was directing the appearance of a variety of functional neurotransmitter receptors. |
| doi_str_mv | 10.1016/0028-3908(84)90262-4 |
| format | Article |
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The cells also responded dose-dependently to glutamate, aspartate and kainate, indicating that this mRNA fraction was directing the appearance of a variety of functional neurotransmitter receptors.</description><identifier>ISSN: 0028-3908</identifier><identifier>DOI: 10.1016/0028-3908(84)90262-4</identifier><language>eng</language><subject>gamma -aminobutyric acid ; glycine ; microinjection ; mRNA ; oocytes ; Xenopus laevis</subject><ispartof>Neuropharmacology, 1984, Vol.23 (7), p.817-818</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c195t-caf34ff4463acc765f4e5f44b8d1077cc4f3d99adff3ae2b67c1681e18c2b23c3</citedby><cites>FETCH-LOGICAL-c195t-caf34ff4463acc765f4e5f44b8d1077cc4f3d99adff3ae2b67c1681e18c2b23c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,4010,27900,27901,27902</link.rule.ids></links><search><creatorcontrib>Constanti, A.</creatorcontrib><creatorcontrib>Houamed, K.M.</creatorcontrib><creatorcontrib>Smart, T.G.</creatorcontrib><creatorcontrib>Bilbe, G.</creatorcontrib><creatorcontrib>Brown, D.A.</creatorcontrib><creatorcontrib>Barnard, E.A.</creatorcontrib><title>Functional brain GABA and glycine receptors in mRNA-injected Xenopus oocytes</title><title>Neuropharmacology</title><description>Changes in membrane potential and conductance evoked by GABA were measured in Xenopus ooctyes injected with chick or rat brain mRNA. 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The cells also responded dose-dependently to glutamate, aspartate and kainate, indicating that this mRNA fraction was directing the appearance of a variety of functional neurotransmitter receptors.</description><subject>gamma -aminobutyric acid</subject><subject>glycine</subject><subject>microinjection</subject><subject>mRNA</subject><subject>oocytes</subject><subject>Xenopus laevis</subject><issn>0028-3908</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1984</creationdate><recordtype>article</recordtype><recordid>eNo9kDFPwzAUhD2ARCn8AwZPCIaAHbuOM4aKFqQIJAQSm-W8PKNUqR3sZOi_p6WI4XTS3emGj5Arzu444-qesVxnomT6RsvbkuUqz-QJmf3HZ-Q8pQ1jTGquZ6ReTR7GLnjb0ybaztN19VBR61v61e-g80gjAg5jiInu2-3bS5V1foMwYks_0YdhSjQE2I2YLsips33Cyz-fk4_V4_vyKatf18_Lqs6Al4sxA-uEdE5KJSxAoRZO4l6y0S1nRQEgnWjL0rbOCYt5owrgSnPkGvImFyDm5Pr4O8TwPWEazbZLgH1vPYYpGS5KpcpC7YfyOIQYUorozBC7rY07w5k54DIHLubAxWhpfnEZKX4AoeFgLw</recordid><startdate>1984</startdate><enddate>1984</enddate><creator>Constanti, A.</creator><creator>Houamed, K.M.</creator><creator>Smart, T.G.</creator><creator>Bilbe, G.</creator><creator>Brown, D.A.</creator><creator>Barnard, E.A.</creator><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>M7Z</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>1984</creationdate><title>Functional brain GABA and glycine receptors in mRNA-injected Xenopus oocytes</title><author>Constanti, A. ; Houamed, K.M. ; Smart, T.G. ; Bilbe, G. ; Brown, D.A. ; Barnard, E.A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c195t-caf34ff4463acc765f4e5f44b8d1077cc4f3d99adff3ae2b67c1681e18c2b23c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1984</creationdate><topic>gamma -aminobutyric acid</topic><topic>glycine</topic><topic>microinjection</topic><topic>mRNA</topic><topic>oocytes</topic><topic>Xenopus laevis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Constanti, A.</creatorcontrib><creatorcontrib>Houamed, K.M.</creatorcontrib><creatorcontrib>Smart, T.G.</creatorcontrib><creatorcontrib>Bilbe, G.</creatorcontrib><creatorcontrib>Brown, D.A.</creatorcontrib><creatorcontrib>Barnard, E.A.</creatorcontrib><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biochemistry Abstracts 1</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Neuropharmacology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Constanti, A.</au><au>Houamed, K.M.</au><au>Smart, T.G.</au><au>Bilbe, G.</au><au>Brown, D.A.</au><au>Barnard, E.A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Functional brain GABA and glycine receptors in mRNA-injected Xenopus oocytes</atitle><jtitle>Neuropharmacology</jtitle><date>1984</date><risdate>1984</risdate><volume>23</volume><issue>7</issue><spage>817</spage><epage>818</epage><pages>817-818</pages><issn>0028-3908</issn><abstract>Changes in membrane potential and conductance evoked by GABA were measured in Xenopus ooctyes injected with chick or rat brain mRNA. GABA responses were antagonized by bicuculline or picrotoxinin but enhanced by chlorazepate or by pentobarbitone. Oocytes injected with rat brain mRNA responded similarly to both GABA and glycine. Strychnine selectively depressed glycine responses, whereas picrotoxinin antagonized both GABA and glycine effects non-specifically. GABA (but not glycine) responses were enhanced by pentobarbitone. The cells also responded dose-dependently to glutamate, aspartate and kainate, indicating that this mRNA fraction was directing the appearance of a variety of functional neurotransmitter receptors.</abstract><doi>10.1016/0028-3908(84)90262-4</doi><tpages>2</tpages></addata></record> |
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| ispartof | Neuropharmacology, 1984, Vol.23 (7), p.817-818 |
| issn | 0028-3908 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_13966976 |
| source | Elsevier ScienceDirect Journals |
| subjects | gamma -aminobutyric acid glycine microinjection mRNA oocytes Xenopus laevis |
| title | Functional brain GABA and glycine receptors in mRNA-injected Xenopus oocytes |
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