Antifungal and Antipatulin Activity of Gluconobacter Oxydans Isolated from Apple Surface
Fungicides are the most common agents used in postharvest treatment of fruit and are the most effective against blue mould, primarily caused by Penicillium expansum. Alternatively, blue mould can be treated with antagonistic microorganisms naturally occurring on fruit, such as the bacterium Gluconob...
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| Veröffentlicht in: | Arhiv za higijenu rada i toksikologiju 2013-06, Vol.64 (2), p.93-98 |
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| creator | Bevardi, Martina Frece, Jadranka Mesarek, Dragana Bošnir, Jasna Mrvčić, Jasna Delaš, Frane Markov, Ksenija |
| description | Fungicides are the most common agents used in postharvest treatment of fruit and are the most effective against blue mould, primarily caused by Penicillium expansum. Alternatively, blue mould can be treated with antagonistic microorganisms naturally occurring on fruit, such as the bacterium Gluconobacter oxydans. The aim of this study was to establish the antifungal potential of the G. oxydans 1J strain isolated from apple surface against Penicillium expansum in culture and apple juice and to compare it with the efficiency of a reference strain G. oxydans ATCC 621H. The highest antifungal activity of G. oxydans 1J was observed between days 3 and 9 with no colony growth, while on day 12, P. expansum colony diameter was reduced to 42.3 % of the control diameter. Although G. oxydans 1J did not fully inhibit mould growth, it showed a high level of efficiency and completely prevented patulin accumulation in apple juice.
Tretiranje voća fungicidima, nakon berbe, uobičajeni je način suzbijanja plave plijesni. Međutim, propadanje voća može se spriječiti i upotrebom antagonističkih mikroorganizama, kao što je bakterija Gluconobacter oxydans. Svrha ovoga rada bila je izolirati prirodnu mikrobnu populaciju s površine jabuka i istražiti moguće inhibitorno djelovanje Gluconobacter oxydans 1J na plavu plijesan, Penicillium expansum, najvažnijeg uzročnika kvarenja jabuka u skladištu. Najveća antifungalna aktivnost bakterije primijećena je između 3. i 9. dana, kada nije zabilježen porast kolonija, a nakon 12. dana promjer kolonije plijesni bio je manji za 42,3 %. Iako istraživana bakterija Gluconobacter oxydans 1J nije u potpunosti inhibirala rast plijesni u jabučnom soku pokazala je visoku razinu učinkovitosti (od 86 % do 95 %). Gluconobacter oxydans 1J djelomično inhibira rast plijesni i u potpunosti biosintezu patulina, ovisno o vremenu i uvjetima uzgoja. |
| doi_str_mv | 10.2478/10004-1254-64-2013-2308 |
| format | Article |
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Tretiranje voća fungicidima, nakon berbe, uobičajeni je način suzbijanja plave plijesni. Međutim, propadanje voća može se spriječiti i upotrebom antagonističkih mikroorganizama, kao što je bakterija Gluconobacter oxydans. Svrha ovoga rada bila je izolirati prirodnu mikrobnu populaciju s površine jabuka i istražiti moguće inhibitorno djelovanje Gluconobacter oxydans 1J na plavu plijesan, Penicillium expansum, najvažnijeg uzročnika kvarenja jabuka u skladištu. Najveća antifungalna aktivnost bakterije primijećena je između 3. i 9. dana, kada nije zabilježen porast kolonija, a nakon 12. dana promjer kolonije plijesni bio je manji za 42,3 %. Iako istraživana bakterija Gluconobacter oxydans 1J nije u potpunosti inhibirala rast plijesni u jabučnom soku pokazala je visoku razinu učinkovitosti (od 86 % do 95 %). Gluconobacter oxydans 1J djelomično inhibira rast plijesni i u potpunosti biosintezu patulina, ovisno o vremenu i uvjetima uzgoja.</description><identifier>ISSN: 0004-1254</identifier><identifier>EISSN: 1848-6312</identifier><identifier>DOI: 10.2478/10004-1254-64-2013-2308</identifier><identifier>PMID: 23819937</identifier><language>eng</language><publisher>Croatia: Versita</publisher><subject>antagonistic microorganisms ; antagonistički mikroorganizmi ; Antifungal Agents ; Food Microbiology ; Gluconobacter oxydans - classification ; Gluconobacter oxydans - physiology ; Malus - microbiology ; mould growth ; nakupljanje toksina ; Patulin - antagonists & inhibitors ; Patulin - biosynthesis ; Penicillium - growth & development ; Penicillium - isolation & purification ; Penicillium expansum ; Pest Control, Biological - methods ; rast plijesni ; Species Specificity ; toxin accumulation</subject><ispartof>Arhiv za higijenu rada i toksikologiju, 2013-06, Vol.64 (2), p.93-98</ispartof><rights>Copyright Institute for Medical Research and Occupational Health 2013</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c587t-dba11f955c396a50f9fb2a10608c5c368befdb08f89a8cd85bff84118d98185e3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.degruyter.com/document/doi/10.2478/10004-1254-64-2013-2308/pdf$$EPDF$$P50$$Gwalterdegruyter$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.degruyter.com/document/doi/10.2478/10004-1254-64-2013-2308/html$$EHTML$$P50$$Gwalterdegruyter$$Hfree_for_read</linktohtml><link.rule.ids>314,776,780,860,27903,27904,66905,68689</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23819937$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bevardi, Martina</creatorcontrib><creatorcontrib>Frece, Jadranka</creatorcontrib><creatorcontrib>Mesarek, Dragana</creatorcontrib><creatorcontrib>Bošnir, Jasna</creatorcontrib><creatorcontrib>Mrvčić, Jasna</creatorcontrib><creatorcontrib>Delaš, Frane</creatorcontrib><creatorcontrib>Markov, Ksenija</creatorcontrib><title>Antifungal and Antipatulin Activity of Gluconobacter Oxydans Isolated from Apple Surface</title><title>Arhiv za higijenu rada i toksikologiju</title><addtitle>Arh Hig Rada Toksikol</addtitle><description>Fungicides are the most common agents used in postharvest treatment of fruit and are the most effective against blue mould, primarily caused by Penicillium expansum. Alternatively, blue mould can be treated with antagonistic microorganisms naturally occurring on fruit, such as the bacterium Gluconobacter oxydans. The aim of this study was to establish the antifungal potential of the G. oxydans 1J strain isolated from apple surface against Penicillium expansum in culture and apple juice and to compare it with the efficiency of a reference strain G. oxydans ATCC 621H. The highest antifungal activity of G. oxydans 1J was observed between days 3 and 9 with no colony growth, while on day 12, P. expansum colony diameter was reduced to 42.3 % of the control diameter. Although G. oxydans 1J did not fully inhibit mould growth, it showed a high level of efficiency and completely prevented patulin accumulation in apple juice.
Tretiranje voća fungicidima, nakon berbe, uobičajeni je način suzbijanja plave plijesni. Međutim, propadanje voća može se spriječiti i upotrebom antagonističkih mikroorganizama, kao što je bakterija Gluconobacter oxydans. Svrha ovoga rada bila je izolirati prirodnu mikrobnu populaciju s površine jabuka i istražiti moguće inhibitorno djelovanje Gluconobacter oxydans 1J na plavu plijesan, Penicillium expansum, najvažnijeg uzročnika kvarenja jabuka u skladištu. Najveća antifungalna aktivnost bakterije primijećena je između 3. i 9. dana, kada nije zabilježen porast kolonija, a nakon 12. dana promjer kolonije plijesni bio je manji za 42,3 %. Iako istraživana bakterija Gluconobacter oxydans 1J nije u potpunosti inhibirala rast plijesni u jabučnom soku pokazala je visoku razinu učinkovitosti (od 86 % do 95 %). Gluconobacter oxydans 1J djelomično inhibira rast plijesni i u potpunosti biosintezu patulina, ovisno o vremenu i uvjetima uzgoja.</description><subject>antagonistic microorganisms</subject><subject>antagonistički mikroorganizmi</subject><subject>Antifungal Agents</subject><subject>Food Microbiology</subject><subject>Gluconobacter oxydans - classification</subject><subject>Gluconobacter oxydans - physiology</subject><subject>Malus - microbiology</subject><subject>mould growth</subject><subject>nakupljanje toksina</subject><subject>Patulin - antagonists & inhibitors</subject><subject>Patulin - biosynthesis</subject><subject>Penicillium - growth & development</subject><subject>Penicillium - isolation & purification</subject><subject>Penicillium expansum</subject><subject>Pest Control, Biological - methods</subject><subject>rast plijesni</subject><subject>Species Specificity</subject><subject>toxin accumulation</subject><issn>0004-1254</issn><issn>1848-6312</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9kE1v1DAQhi1ERZfCXwBLXHoJ9Vec8XFVoFSq1EOLxM1y_FGl8sbBjgv775uwpUIcONkePTPz-kHoPSUfmejgjBJCRENZKxopGkYobxgn8AJtKAhoJKfsJdo8Q8fodSn3hLRCgXqFjhkHqhTvNuj7dpyHUMc7E7EZHV6fk5lrHEa8tfPwMMx7nAK-iNWmMfXGzj7j6197Z8aCL0uKZvYOh5x2eDtN0eObmoOx_g06CiYW__bpPEHfvny-Pf_aXF1fXJ5vrxrbQjc3rjeUBtW2litpWhJU6JmhRBKwS01C74PrCQRQBqyDtg8BBKXgFFBoPT9Bp4e5U04_qi-z3g3F-hjN6FMtmnLFgS0_lwv64R_0PtU8Luk0FYJ1QlLCF6o7UDanUrIPesrDzuS9pkSv8vVv-Xr1qqXQq3y9yl863z3Nr_3Ou-e-P7YX4NMB-GniotH5u1z3y-WvHP9fIQVTnD8Cx_OUZg</recordid><startdate>20130601</startdate><enddate>20130601</enddate><creator>Bevardi, Martina</creator><creator>Frece, Jadranka</creator><creator>Mesarek, Dragana</creator><creator>Bošnir, Jasna</creator><creator>Mrvčić, Jasna</creator><creator>Delaš, Frane</creator><creator>Markov, Ksenija</creator><general>Versita</general><general>Institute for Medical Research and Occupational Health</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>BYOGL</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>PATMY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PYCSY</scope><scope>7X8</scope></search><sort><creationdate>20130601</creationdate><title>Antifungal and Antipatulin Activity of Gluconobacter Oxydans Isolated from Apple Surface</title><author>Bevardi, Martina ; 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Alternatively, blue mould can be treated with antagonistic microorganisms naturally occurring on fruit, such as the bacterium Gluconobacter oxydans. The aim of this study was to establish the antifungal potential of the G. oxydans 1J strain isolated from apple surface against Penicillium expansum in culture and apple juice and to compare it with the efficiency of a reference strain G. oxydans ATCC 621H. The highest antifungal activity of G. oxydans 1J was observed between days 3 and 9 with no colony growth, while on day 12, P. expansum colony diameter was reduced to 42.3 % of the control diameter. Although G. oxydans 1J did not fully inhibit mould growth, it showed a high level of efficiency and completely prevented patulin accumulation in apple juice.
Tretiranje voća fungicidima, nakon berbe, uobičajeni je način suzbijanja plave plijesni. Međutim, propadanje voća može se spriječiti i upotrebom antagonističkih mikroorganizama, kao što je bakterija Gluconobacter oxydans. Svrha ovoga rada bila je izolirati prirodnu mikrobnu populaciju s površine jabuka i istražiti moguće inhibitorno djelovanje Gluconobacter oxydans 1J na plavu plijesan, Penicillium expansum, najvažnijeg uzročnika kvarenja jabuka u skladištu. Najveća antifungalna aktivnost bakterije primijećena je između 3. i 9. dana, kada nije zabilježen porast kolonija, a nakon 12. dana promjer kolonije plijesni bio je manji za 42,3 %. Iako istraživana bakterija Gluconobacter oxydans 1J nije u potpunosti inhibirala rast plijesni u jabučnom soku pokazala je visoku razinu učinkovitosti (od 86 % do 95 %). Gluconobacter oxydans 1J djelomično inhibira rast plijesni i u potpunosti biosintezu patulina, ovisno o vremenu i uvjetima uzgoja.</abstract><cop>Croatia</cop><pub>Versita</pub><pmid>23819937</pmid><doi>10.2478/10004-1254-64-2013-2308</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Arhiv za higijenu rada i toksikologiju, 2013-06, Vol.64 (2), p.93-98 |
| issn | 0004-1254 1848-6312 |
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| source | MEDLINE; De Gruyter Open Access Journals; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | antagonistic microorganisms antagonistički mikroorganizmi Antifungal Agents Food Microbiology Gluconobacter oxydans - classification Gluconobacter oxydans - physiology Malus - microbiology mould growth nakupljanje toksina Patulin - antagonists & inhibitors Patulin - biosynthesis Penicillium - growth & development Penicillium - isolation & purification Penicillium expansum Pest Control, Biological - methods rast plijesni Species Specificity toxin accumulation |
| title | Antifungal and Antipatulin Activity of Gluconobacter Oxydans Isolated from Apple Surface |
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