The periodic loss of metabolically unstable DNA during replication of chromosomal DNA of CHO cells
The fate of 3H-thymidine incorporated into newly synthesized DNA of CHO cells was analyzed by either the estimation of the incorporated radioactivity per cell or sedimentation in alkaline sucrose gradient. Under conditions in which DNA synthesis proceeded continuously, of incorporated radioactivity...
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| Veröffentlicht in: | Biochemical and biophysical research communications 1984-03, Vol.119 (3), p.920-925 |
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| container_title | Biochemical and biophysical research communications |
| container_volume | 119 |
| creator | Masuda, Michiyoshi Isiglo, Kimio Yamoka, Hideki Matsumoto, Takafumi Takahashi, Manabu |
| description | The fate of 3H-thymidine incorporated into newly synthesized DNA of CHO cells was analyzed by either the estimation of the incorporated radioactivity per cell or sedimentation in alkaline sucrose gradient. Under conditions in which DNA synthesis proceeded continuously, of incorporated radioactivity was periodically lost and regained during a 90 min chase, corresponding to a cyclic change in the sedimentation profiles. When DNA synthesis was inhibited by hydroxyurea no cyclic change of the incorporated radioactivity was observed. The cyclic changes were regarded as the result of an actual metabolic change in3H-labelled DNA probaly joining to one of the newly formed sister strands of DNA and the loss of radioactivity seems to require active continued DNA synthesis. |
| doi_str_mv | 10.1016/0006-291X(84)90861-1 |
| format | Article |
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Under conditions in which DNA synthesis proceeded continuously, of incorporated radioactivity was periodically lost and regained during a 90 min chase, corresponding to a cyclic change in the sedimentation profiles. When DNA synthesis was inhibited by hydroxyurea no cyclic change of the incorporated radioactivity was observed. The cyclic changes were regarded as the result of an actual metabolic change in3H-labelled DNA probaly joining to one of the newly formed sister strands of DNA and the loss of radioactivity seems to require active continued DNA synthesis.</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1016/0006-291X(84)90861-1</identifier><identifier>PMID: 6712677</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Animals ; Cell Line ; Cricetinae ; Cricetulus ; DNA - genetics ; DNA - isolation & purification ; DNA - metabolism ; DNA Replication - drug effects ; Female ; Hydroxyurea - toxicity ; Kinetics ; Ovary</subject><ispartof>Biochemical and biophysical research communications, 1984-03, Vol.119 (3), p.920-925</ispartof><rights>1984 Academic Press, Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c337t-bfbf7b418494ec8004794779c93f68f82bd0d1e16883402f5184363e6cea9ceb3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/0006291X84908611$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27903,27904,65309</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/6712677$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Masuda, Michiyoshi</creatorcontrib><creatorcontrib>Isiglo, Kimio</creatorcontrib><creatorcontrib>Yamoka, Hideki</creatorcontrib><creatorcontrib>Matsumoto, Takafumi</creatorcontrib><creatorcontrib>Takahashi, Manabu</creatorcontrib><title>The periodic loss of metabolically unstable DNA during replication of chromosomal DNA of CHO cells</title><title>Biochemical and biophysical research communications</title><addtitle>Biochem Biophys Res Commun</addtitle><description>The fate of 3H-thymidine incorporated into newly synthesized DNA of CHO cells was analyzed by either the estimation of the incorporated radioactivity per cell or sedimentation in alkaline sucrose gradient. Under conditions in which DNA synthesis proceeded continuously, of incorporated radioactivity was periodically lost and regained during a 90 min chase, corresponding to a cyclic change in the sedimentation profiles. When DNA synthesis was inhibited by hydroxyurea no cyclic change of the incorporated radioactivity was observed. The cyclic changes were regarded as the result of an actual metabolic change in3H-labelled DNA probaly joining to one of the newly formed sister strands of DNA and the loss of radioactivity seems to require active continued DNA synthesis.</description><subject>Animals</subject><subject>Cell Line</subject><subject>Cricetinae</subject><subject>Cricetulus</subject><subject>DNA - genetics</subject><subject>DNA - isolation & purification</subject><subject>DNA - metabolism</subject><subject>DNA Replication - drug effects</subject><subject>Female</subject><subject>Hydroxyurea - toxicity</subject><subject>Kinetics</subject><subject>Ovary</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1984</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE9LxDAQxYMouq5-A4WcRA_VTBvT5CLI-hdELwreQptONZI2a9IKfntTd_Hoaci835vJPEIOgJ0CA3HGGBNZruD1WPITxaSADDbIDJhiWQ6Mb5LZH7JDdmP8YAyAC7VNtkUJuSjLGamf35EuMVjfWEOdj5H6lnY4VLV31lTOfdOxj-npkF49XtJmDLZ_owGXkzxY308G8x5856PvKvdLpdbi7okadC7uka22chH313VOXm6unxd32cPT7f3i8iEzRVEOWd3WbVlzkFxxNJIxXipelsqoohWylXndsAYQhJQFZ3l7nshCFCgMVspgXczJ0WruMvjPEeOgOxunH1Q9-jFqKBTwZE8gX4EmpHsDtnoZbFeFbw1MT9HqKTc95aYl17_RJvecHK7nj3WHzZ9pnWXSL1Y6piO_LAYdjcXeYGMDmkE33v6_4AcavIfk</recordid><startdate>19840330</startdate><enddate>19840330</enddate><creator>Masuda, Michiyoshi</creator><creator>Isiglo, Kimio</creator><creator>Yamoka, Hideki</creator><creator>Matsumoto, Takafumi</creator><creator>Takahashi, Manabu</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>19840330</creationdate><title>The periodic loss of metabolically unstable DNA during replication of chromosomal DNA of CHO cells</title><author>Masuda, Michiyoshi ; Isiglo, Kimio ; Yamoka, Hideki ; Matsumoto, Takafumi ; Takahashi, Manabu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c337t-bfbf7b418494ec8004794779c93f68f82bd0d1e16883402f5184363e6cea9ceb3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1984</creationdate><topic>Animals</topic><topic>Cell Line</topic><topic>Cricetinae</topic><topic>Cricetulus</topic><topic>DNA - genetics</topic><topic>DNA - isolation & purification</topic><topic>DNA - metabolism</topic><topic>DNA Replication - drug effects</topic><topic>Female</topic><topic>Hydroxyurea - toxicity</topic><topic>Kinetics</topic><topic>Ovary</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Masuda, Michiyoshi</creatorcontrib><creatorcontrib>Isiglo, Kimio</creatorcontrib><creatorcontrib>Yamoka, Hideki</creatorcontrib><creatorcontrib>Matsumoto, Takafumi</creatorcontrib><creatorcontrib>Takahashi, Manabu</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Masuda, Michiyoshi</au><au>Isiglo, Kimio</au><au>Yamoka, Hideki</au><au>Matsumoto, Takafumi</au><au>Takahashi, Manabu</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The periodic loss of metabolically unstable DNA during replication of chromosomal DNA of CHO cells</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>1984-03-30</date><risdate>1984</risdate><volume>119</volume><issue>3</issue><spage>920</spage><epage>925</epage><pages>920-925</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><abstract>The fate of 3H-thymidine incorporated into newly synthesized DNA of CHO cells was analyzed by either the estimation of the incorporated radioactivity per cell or sedimentation in alkaline sucrose gradient. Under conditions in which DNA synthesis proceeded continuously, of incorporated radioactivity was periodically lost and regained during a 90 min chase, corresponding to a cyclic change in the sedimentation profiles. When DNA synthesis was inhibited by hydroxyurea no cyclic change of the incorporated radioactivity was observed. The cyclic changes were regarded as the result of an actual metabolic change in3H-labelled DNA probaly joining to one of the newly formed sister strands of DNA and the loss of radioactivity seems to require active continued DNA synthesis.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>6712677</pmid><doi>10.1016/0006-291X(84)90861-1</doi><tpages>6</tpages></addata></record> |
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| ispartof | Biochemical and biophysical research communications, 1984-03, Vol.119 (3), p.920-925 |
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| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Animals Cell Line Cricetinae Cricetulus DNA - genetics DNA - isolation & purification DNA - metabolism DNA Replication - drug effects Female Hydroxyurea - toxicity Kinetics Ovary |
| title | The periodic loss of metabolically unstable DNA during replication of chromosomal DNA of CHO cells |
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