A W.H.O. collaborative study of in vitro and in vivo methods for the assay of yellow fever vaccines
An international collaborative study was carried out to compare the plaque assay in monolayer cell cultures with the mouse assay for the potency testing of yellow fever vaccines. Twelve laboratories assayed four preparations of yellow fever virus by both methods. Differences were found between estim...
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| Veröffentlicht in: | Journal of biological standardization 1983, Vol.11 (1), p.47-54 |
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| container_title | Journal of biological standardization |
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| creator | Seagroatt, Valerie Magrath, D.I. |
| description | An international collaborative study was carried out to compare the plaque assay in monolayer cell cultures with the mouse assay for the potency testing of yellow fever vaccines. Twelve laboratories assayed four preparations of yellow fever virus by both methods. Differences were found between estimates of infectivities obtained by different laboratories using the plaque assay. There was also large variation between the estimates of mouse LD
50 obtained by different laboratories. However, expressing potencies of the preparations relative to a common preparation greatly reduced the variation between laboratories for both the plaque and the mouse tests; it also resulted in remarkably close agreement between the methods. |
| doi_str_mv | 10.1016/S0092-1157(83)80045-6 |
| format | Article |
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| fulltext | fulltext |
| identifier | ISSN: 0092-1157 |
| ispartof | Journal of biological standardization, 1983, Vol.11 (1), p.47-54 |
| issn | 0092-1157 |
| language | eng |
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| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Animals Cells, Cultured Mice Viral Plaque Assay Viral Vaccines - immunology Viral Vaccines - toxicity World Health Organization yellow fever virus Yellow fever virus - immunology Yellow fever virus - pathogenicity |
| title | A W.H.O. collaborative study of in vitro and in vivo methods for the assay of yellow fever vaccines |
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