The complete amino acid sequence of hirudin, a thrombin specific inhibitor: Application of colour carboxymethylation
Color carboxymethylation of cysteine residues with a new chromophoric reagent dimethylaminoazobenzene iodoacetamide, was applied to the micro-sequence analysis of hirudin, a thrombin specific inhibitor. Six cysteine residues of the reduced hirudin were detected as colored phenylthiohydantoin derivat...
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| Veröffentlicht in: | FEBS letters 1984-01, Vol.165 (2), p.180-184 |
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| creator | Dodt, Johannes Müller, Hans-Peter Seemüller, Ursula Chang, Jui-Yoa |
| description | Color carboxymethylation of cysteine residues with a new chromophoric reagent dimethylaminoazobenzene iodoacetamide, was applied to the micro-sequence analysis of hirudin, a thrombin specific inhibitor. Six cysteine residues of the reduced hirudin were detected as colored phenylthiohydantoin derivative and 3 tryptic peptides of hirudin (all containing cysteines) were isolated as colored peptide. The complete hirudin sequence, including 6 uncertain positions left in the previous report [Petersen T.E. (1976) in: Protides of the Biological Fluids; 23rd Colloquium, pp. 145, Pergamon Press, London] was established. |
| doi_str_mv | 10.1016/0014-5793(84)80165-9 |
| format | Article |
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Six cysteine residues of the reduced hirudin were detected as colored phenylthiohydantoin derivative and 3 tryptic peptides of hirudin (all containing cysteines) were isolated as colored peptide. The complete hirudin sequence, including 6 uncertain positions left in the previous report [Petersen T.E. (1976) in: Protides of the Biological Fluids; 23rd Colloquium, pp. 145, Pergamon Press, London] was established.</description><subject>amino acid sequence</subject><subject>Color carboxymethylation</subject><subject>Hirudin</subject><subject>Hirudo medicinalis</subject><subject>salivary gland</subject><subject>thrombin inhibitor</subject><subject>Tyrosine sulfate</subject><issn>0014-5793</issn><issn>1873-3468</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1984</creationdate><recordtype>article</recordtype><recordid>eNp9kE9LxDAQxYMouK5-Aw85iYLVpE23qQdBxH8geNFzSKdTOtI2NcmK--1tXfHoaZiZ9x68H2PHUlxIIVeXQkiV5EWZnWp1pqdLnpQ7bCF1kSWZWuldtviT7LODEN7FtGtZLlh8bZGD68cOI3Lb0-C4Bap5wI81DoDcNbwlv65pOOeWx9a7vqKBhxGBGgJOQ0sVReev-M04dgQ2khtmG7jOrT0H6yv3tekxtpvu53nI9hrbBTz6nUv2dn_3evuYPL88PN3ePCeQahUTFHmKWuUrLEFhmleiycDCStXTRTSqsKWqMK0qlQuYtRYLleaYKZXWWS2zJTvZ5o7eTW1CND0FwK6zA7p1MDLTUqaymIRqKwTvQvDYmNFTb_3GSGFmxGbmZ2Z-Rivzg9iUk-16a8OpxCehNwFohlaTR4imdvR_wDdrGoUI</recordid><startdate>19840101</startdate><enddate>19840101</enddate><creator>Dodt, Johannes</creator><creator>Müller, Hans-Peter</creator><creator>Seemüller, Ursula</creator><creator>Chang, Jui-Yoa</creator><general>Elsevier B.V</general><scope>6I.</scope><scope>AAFTH</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>C1K</scope></search><sort><creationdate>19840101</creationdate><title>The complete amino acid sequence of hirudin, a thrombin specific inhibitor: Application of colour carboxymethylation</title><author>Dodt, Johannes ; Müller, Hans-Peter ; Seemüller, Ursula ; Chang, Jui-Yoa</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c284t-e052e8456e9c4e25b0f3cac64d6e90f47a94be2bb450c052eae7425e3442d3d13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1984</creationdate><topic>amino acid sequence</topic><topic>Color carboxymethylation</topic><topic>Hirudin</topic><topic>Hirudo medicinalis</topic><topic>salivary gland</topic><topic>thrombin inhibitor</topic><topic>Tyrosine sulfate</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Dodt, Johannes</creatorcontrib><creatorcontrib>Müller, Hans-Peter</creatorcontrib><creatorcontrib>Seemüller, Ursula</creatorcontrib><creatorcontrib>Chang, Jui-Yoa</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><jtitle>FEBS letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Dodt, Johannes</au><au>Müller, Hans-Peter</au><au>Seemüller, Ursula</au><au>Chang, Jui-Yoa</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The complete amino acid sequence of hirudin, a thrombin specific inhibitor: Application of colour carboxymethylation</atitle><jtitle>FEBS letters</jtitle><date>1984-01-01</date><risdate>1984</risdate><volume>165</volume><issue>2</issue><spage>180</spage><epage>184</epage><pages>180-184</pages><issn>0014-5793</issn><eissn>1873-3468</eissn><abstract>Color carboxymethylation of cysteine residues with a new chromophoric reagent dimethylaminoazobenzene iodoacetamide, was applied to the micro-sequence analysis of hirudin, a thrombin specific inhibitor. Six cysteine residues of the reduced hirudin were detected as colored phenylthiohydantoin derivative and 3 tryptic peptides of hirudin (all containing cysteines) were isolated as colored peptide. The complete hirudin sequence, including 6 uncertain positions left in the previous report [Petersen T.E. (1976) in: Protides of the Biological Fluids; 23rd Colloquium, pp. 145, Pergamon Press, London] was established.</abstract><pub>Elsevier B.V</pub><doi>10.1016/0014-5793(84)80165-9</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | FEBS letters, 1984-01, Vol.165 (2), p.180-184 |
| issn | 0014-5793 1873-3468 |
| language | eng |
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| source | Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; ScienceDirect Journals (5 years ago - present); Alma/SFX Local Collection |
| subjects | amino acid sequence Color carboxymethylation Hirudin Hirudo medicinalis salivary gland thrombin inhibitor Tyrosine sulfate |
| title | The complete amino acid sequence of hirudin, a thrombin specific inhibitor: Application of colour carboxymethylation |
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