Enterically induced regulation of systemic immune responses. II. Suppression of proliferating T cells by an Lyt-1 super(+), 2 super(-) T effector cell

Peripheral lymph node cells from C3H mice that were fed and injected with bovine serum albumin (REG cells) demonstrate an impaired proliferative response to antigenic stimulation in vitro compared to cells from mice only injected with BSA. To determine whether suppressor cells contributed to this enterically induced impairment of systemic T cell responses, REG cells were pretreated with various monoclonal antibodies and complement (C), and were then co-cultured with antigen-reactive indicator T cell (IND) from parenterally immunized mice. Proliferation of IND cells (( super(3)H)thymidine uptake) was suppressed only if REG cells were treated with anti-Lyt-2 and C before coculture. The ability of anti-Lyt-1 plus anti-Lyt-2 and C treatment to abrogate suppression suggested that the suppressor effect was due to an Lyt-1 super(+), 2 super(-) REG cell. Suppression was independent of Lyt-2 super(+) IND cells, and was observed at different antigen concentrations, cultivation times, and cell densities. The cells responsible for suppressor activity were radiosensitive, nylon wool non-adherent, and antigen specific. The data suggests that an Lyt-1 super(+), 2 super(-) T cell could be an important component in mediating enterically induced regulation of systemic T cell responses.