Hydrophobic interaction of ribosomal ribonucleic acids on a nitrocellulose column
The non-covalent attachment of polynucleotides to polysaccharide matrices has been extensively used in quantitative biology, starting with the DNA-agar techqnique. Thus, nitroceulluose can effect an interaction with single stranded DNA, poly(A)+mRNAs and poly(A). However, these is little information...
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| Veröffentlicht in: | Journal of Chromatography A 1983-01, Vol.268 (2), p.285-290 |
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| container_end_page | 290 |
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| container_issue | 2 |
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| container_title | Journal of Chromatography A |
| container_volume | 268 |
| creator | Popović, D.A. Wintzerith, M. |
| description | The non-covalent attachment of polynucleotides to polysaccharide matrices has been extensively used in quantitative biology, starting with the DNA-agar techqnique. Thus, nitroceulluose can effect an interaction with single stranded DNA, poly(A)+mRNAs and poly(A). However, these is little information on the retention of rRNA subunits on a nitrocellulose column. The authors have recently described conditions resulting in selective retention of the larger (28S) rat liver rRNA on a nitrocellulose column. The main purpose of this communication is to compare nitrocellulose with other polysaccharide matrices used previously for selective or less selective retention of individual rRNA species (Sepharoses, agaroses and agar). Rat liver rRNAs, pea seedling rRNAs and Escherichia coli rRNAs were used. |
| doi_str_mv | 10.1016/S0021-9673(01)95417-2 |
| format | Article |
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Psychology</subject><subject>Liver - analysis</subject><subject>Molecular Weight</subject><subject>Nucleic acids</subject><subject>Pisum sativum</subject><subject>Plants, Medicinal</subject><subject>Rats</subject><subject>Rna, ribonucleoproteins</subject><subject>RNA, Ribosomal - isolation & purification</subject><subject>Temperature</subject><issn>0021-9673</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1983</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkEtLw0AQgPeg1Fr9CYUcRPQQ3Ucem5NIUSsURNTzspmd4EqSrbuJ0H9vYkOvnmaY-ebBR8iS0RtGWXb7RilncZHl4oqy6yJNWB7zIzI_lE_IaQhflLKc5nxGZplIJRfFnLyud8a77acrLUS27dBr6KxrI1dF3pYuuEbXf1nbQ40DpMGaEA2EjlrbeQdY133tAkbg6r5pz8hxpeuA51NckI_Hh_fVOt68PD2v7jcxCFl0MfIEZF6JRGqdVyVDrpEXSUlLU2ouDRRS5BQBQRoUQmcMk0yUkoEsjIFULMjlfu_Wu-8eQ6caG8ZndIuuD4qJPEkKPoLpHgTvQvBYqa23jfY7xaga9ak_fWr0pOgQR32KD3PL6UBfNmgOU5O7oX8x9XUAXVdet2DDARvXcTGev9tjOMj4sehVAIstoLEeoVPG2X8e-QVTVpAH</recordid><startdate>19830101</startdate><enddate>19830101</enddate><creator>Popović, D.A.</creator><creator>Wintzerith, M.</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope></search><sort><creationdate>19830101</creationdate><title>Hydrophobic interaction of ribosomal ribonucleic acids on a nitrocellulose column</title><author>Popović, D.A. ; Wintzerith, M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c389t-e24c87f348aa7fb1e2ae294b0bdba28dc98370ecec8de33a61e463b81c89ddc53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1983</creationdate><topic>Analytical, structural and metabolic biochemistry</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Chemical Phenomena</topic><topic>Chemistry</topic><topic>Chromatography - methods</topic><topic>Collodion</topic><topic>Escherichia coli</topic><topic>Escherichia coli - analysis</topic><topic>Fabaceae - analysis</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Liver - analysis</topic><topic>Molecular Weight</topic><topic>Nucleic acids</topic><topic>Pisum sativum</topic><topic>Plants, Medicinal</topic><topic>Rats</topic><topic>Rna, ribonucleoproteins</topic><topic>RNA, Ribosomal - isolation & purification</topic><topic>Temperature</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Popović, D.A.</creatorcontrib><creatorcontrib>Wintzerith, M.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><jtitle>Journal of Chromatography A</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Popović, D.A.</au><au>Wintzerith, M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Hydrophobic interaction of ribosomal ribonucleic acids on a nitrocellulose column</atitle><jtitle>Journal of Chromatography A</jtitle><addtitle>J Chromatogr</addtitle><date>1983-01-01</date><risdate>1983</risdate><volume>268</volume><issue>2</issue><spage>285</spage><epage>290</epage><pages>285-290</pages><issn>0021-9673</issn><coden>JOCRAM</coden><abstract>The non-covalent attachment of polynucleotides to polysaccharide matrices has been extensively used in quantitative biology, starting with the DNA-agar techqnique. 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| ispartof | Journal of Chromatography A, 1983-01, Vol.268 (2), p.285-290 |
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| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Analytical, structural and metabolic biochemistry Animals Biological and medical sciences Chemical Phenomena Chemistry Chromatography - methods Collodion Escherichia coli Escherichia coli - analysis Fabaceae - analysis Fundamental and applied biological sciences. Psychology Liver - analysis Molecular Weight Nucleic acids Pisum sativum Plants, Medicinal Rats Rna, ribonucleoproteins RNA, Ribosomal - isolation & purification Temperature |
| title | Hydrophobic interaction of ribosomal ribonucleic acids on a nitrocellulose column |
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