Fluorescent detection of protein kinase based on zirconium ions-immobilized magnetic nanoparticles

•Present a novel method for PKA activity analysis based on magnetic separation.•Zr-NTA MNPs are implemented in detection of PKA activation in cell lysates.•Specific adsorption between zirconium ions and phosphate group.•Highly selective, sensitive and fast recognition of phosphorylated peptides.•The...

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Veröffentlicht in:	Analytica chimica acta 2013-05, Vol.780, p.89-94
Hauptverfasser:	Tan, Penglong, Lei, Chunyang, Liu, Xin, Qing, Meng, Nie, Zhou, Guo, Manli, Huang, Yan, Yao, Shouzhuo
Format:	Artikel
Sprache:	eng
Schlagworte:	Adsorption Assaying Cell Line, Tumor Fluorescence Fluorometric method Humans Inhibitors Kinases Magnetic separation Magnetics Nanoparticles Nanoparticles - chemistry Peptides Phosphorylation Protein Binding Protein kinase Protein Kinases - chemistry Protein Kinases - metabolism Proteins Zirconium Zirconium - chemistry Zirconium ions-immobilized magnetic nanoparticles
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