MicroRNA-181b expression in prostate cancer tissues and its influence on the biological behavior of the prostate cancer cell line PC-3
We examined microRNA-181b (miRNA) expression in prostate cancer tissues and its effect on the prostate cancer cell line PC-3. Tissues from 27 cases of prostate cancer and 30 samples of normal human prostate were collected by surgical removal. Total miRNA was extracted, and the relative expression of...
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| Veröffentlicht in: | Genetics and molecular research 2013-04, Vol.12 (2), p.1012-1021 |
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| creator | He, L Yao, H Fan, L H Liu, L Qiu, S Li, X Gao, J P Hao, C Q |
| description | We examined microRNA-181b (miRNA) expression in prostate cancer tissues and its effect on the prostate cancer cell line PC-3. Tissues from 27 cases of prostate cancer and 30 samples of normal human prostate were collected by surgical removal. Total miRNA was extracted, and the relative expression of miR-181b was quantified using RT-PCR. miR-181b ASO was transfected into prostate cancer PC-3 cells. miR-181b expression in transfected and non-transfected cells was measured using RT-PCR. Changes in cell apoptosis were measured using flow cytometry. MTT and cell growth curve methods were used to assess the influence of miR-181b expression on cell proliferation. The changes in cell invasive ability in vitro were detected using the Transwell chamber method. miR-181b was up-regulated in the prostate cancer tissues compared with the normal prostate samples. It was down-regulated after miR-181b ASO transfection into the prostate cancer PC-3 cells. Down-regulation of miR-181b in the PC-3 cell induced apoptosis, inhibited proliferation, and depressed invasion of PC-3 cells in vitro. As miR-181b is over-expressed in prostate cancer, its down-regulation could have potential as gene therapy for prostate cancer by inducing apoptosis, inhibiting proliferation and depressing invasion by cancer cells. |
| doi_str_mv | 10.4238/2013.April.2.17 |
| format | Article |
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Tissues from 27 cases of prostate cancer and 30 samples of normal human prostate were collected by surgical removal. Total miRNA was extracted, and the relative expression of miR-181b was quantified using RT-PCR. miR-181b ASO was transfected into prostate cancer PC-3 cells. miR-181b expression in transfected and non-transfected cells was measured using RT-PCR. Changes in cell apoptosis were measured using flow cytometry. MTT and cell growth curve methods were used to assess the influence of miR-181b expression on cell proliferation. The changes in cell invasive ability in vitro were detected using the Transwell chamber method. miR-181b was up-regulated in the prostate cancer tissues compared with the normal prostate samples. It was down-regulated after miR-181b ASO transfection into the prostate cancer PC-3 cells. Down-regulation of miR-181b in the PC-3 cell induced apoptosis, inhibited proliferation, and depressed invasion of PC-3 cells in vitro. As miR-181b is over-expressed in prostate cancer, its down-regulation could have potential as gene therapy for prostate cancer by inducing apoptosis, inhibiting proliferation and depressing invasion by cancer cells.</description><identifier>ISSN: 1676-5680</identifier><identifier>EISSN: 1676-5680</identifier><identifier>DOI: 10.4238/2013.April.2.17</identifier><identifier>PMID: 23613247</identifier><language>eng</language><publisher>Brazil</publisher><subject>Apoptosis - genetics ; Cell Line, Tumor ; Cell Movement - genetics ; Cell Proliferation ; Gene Expression ; Gene Expression Regulation, Neoplastic ; Humans ; Male ; MicroRNAs - genetics ; MicroRNAs - metabolism ; Prostatic Neoplasms - genetics ; Prostatic Neoplasms - metabolism ; Transfection</subject><ispartof>Genetics and molecular research, 2013-04, Vol.12 (2), p.1012-1021</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c330t-f37765338a3bfc78b2eae765ca513a9acaf0c3f38c8cb3d9fdcd1dec654aa2df3</citedby><cites>FETCH-LOGICAL-c330t-f37765338a3bfc78b2eae765ca513a9acaf0c3f38c8cb3d9fdcd1dec654aa2df3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23613247$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>He, L</creatorcontrib><creatorcontrib>Yao, H</creatorcontrib><creatorcontrib>Fan, L H</creatorcontrib><creatorcontrib>Liu, L</creatorcontrib><creatorcontrib>Qiu, S</creatorcontrib><creatorcontrib>Li, X</creatorcontrib><creatorcontrib>Gao, J P</creatorcontrib><creatorcontrib>Hao, C Q</creatorcontrib><title>MicroRNA-181b expression in prostate cancer tissues and its influence on the biological behavior of the prostate cancer cell line PC-3</title><title>Genetics and molecular research</title><addtitle>Genet Mol Res</addtitle><description>We examined microRNA-181b (miRNA) expression in prostate cancer tissues and its effect on the prostate cancer cell line PC-3. Tissues from 27 cases of prostate cancer and 30 samples of normal human prostate were collected by surgical removal. Total miRNA was extracted, and the relative expression of miR-181b was quantified using RT-PCR. miR-181b ASO was transfected into prostate cancer PC-3 cells. miR-181b expression in transfected and non-transfected cells was measured using RT-PCR. Changes in cell apoptosis were measured using flow cytometry. MTT and cell growth curve methods were used to assess the influence of miR-181b expression on cell proliferation. The changes in cell invasive ability in vitro were detected using the Transwell chamber method. miR-181b was up-regulated in the prostate cancer tissues compared with the normal prostate samples. It was down-regulated after miR-181b ASO transfection into the prostate cancer PC-3 cells. Down-regulation of miR-181b in the PC-3 cell induced apoptosis, inhibited proliferation, and depressed invasion of PC-3 cells in vitro. As miR-181b is over-expressed in prostate cancer, its down-regulation could have potential as gene therapy for prostate cancer by inducing apoptosis, inhibiting proliferation and depressing invasion by cancer cells.</description><subject>Apoptosis - genetics</subject><subject>Cell Line, Tumor</subject><subject>Cell Movement - genetics</subject><subject>Cell Proliferation</subject><subject>Gene Expression</subject><subject>Gene Expression Regulation, Neoplastic</subject><subject>Humans</subject><subject>Male</subject><subject>MicroRNAs - genetics</subject><subject>MicroRNAs - metabolism</subject><subject>Prostatic Neoplasms - genetics</subject><subject>Prostatic Neoplasms - metabolism</subject><subject>Transfection</subject><issn>1676-5680</issn><issn>1676-5680</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkctOwzAQRS0EoqWwZoe8ZJNie5LYXVYVL6k8hGBtOc6YGqVJiVMEP8B34z5AiBUrW57jq5k5hBxzNkwFqDPBOAzHi9ZXQzHkcof0eS7zJMsV2_1175GDEF4YE1mq2D7pCcg5iFT2yeeNt23zcDtOuOIFxfdFiyH4pqa-pou2CZ3pkFpTW2xp50NYYqCmLqnvQkRctcRYopHvZkgL31TNs7emogXOzJtvWtq4delvlsWqopWvkd5PEjgke85UAY-254A8XZw_Tq6S6d3l9WQ8TSwA6xIHUuYZgDJQOCtVIdBgfLEm42BGxhrHLDhQVtkCypErbclLtHmWGiNKBwNyusmN7bzGUTo992HViqmxWQbNQQqWM5Wyf6BpzrkciTSiZxs0rjKEFp2ORuam_dCc6ZUnvfKk15600FzGHyfb8GUxx_KH_xYDXzTrkNA</recordid><startdate>20130402</startdate><enddate>20130402</enddate><creator>He, L</creator><creator>Yao, H</creator><creator>Fan, L H</creator><creator>Liu, L</creator><creator>Qiu, S</creator><creator>Li, X</creator><creator>Gao, J P</creator><creator>Hao, C Q</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>20130402</creationdate><title>MicroRNA-181b expression in prostate cancer tissues and its influence on the biological behavior of the prostate cancer cell line PC-3</title><author>He, L ; Yao, H ; Fan, L H ; Liu, L ; Qiu, S ; Li, X ; Gao, J P ; Hao, C Q</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c330t-f37765338a3bfc78b2eae765ca513a9acaf0c3f38c8cb3d9fdcd1dec654aa2df3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Apoptosis - genetics</topic><topic>Cell Line, Tumor</topic><topic>Cell Movement - genetics</topic><topic>Cell Proliferation</topic><topic>Gene Expression</topic><topic>Gene Expression Regulation, Neoplastic</topic><topic>Humans</topic><topic>Male</topic><topic>MicroRNAs - genetics</topic><topic>MicroRNAs - metabolism</topic><topic>Prostatic Neoplasms - genetics</topic><topic>Prostatic Neoplasms - metabolism</topic><topic>Transfection</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>He, L</creatorcontrib><creatorcontrib>Yao, H</creatorcontrib><creatorcontrib>Fan, L H</creatorcontrib><creatorcontrib>Liu, L</creatorcontrib><creatorcontrib>Qiu, S</creatorcontrib><creatorcontrib>Li, X</creatorcontrib><creatorcontrib>Gao, J P</creatorcontrib><creatorcontrib>Hao, C Q</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Genetics and molecular research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>He, L</au><au>Yao, H</au><au>Fan, L H</au><au>Liu, L</au><au>Qiu, S</au><au>Li, X</au><au>Gao, J P</au><au>Hao, C Q</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>MicroRNA-181b expression in prostate cancer tissues and its influence on the biological behavior of the prostate cancer cell line PC-3</atitle><jtitle>Genetics and molecular research</jtitle><addtitle>Genet Mol Res</addtitle><date>2013-04-02</date><risdate>2013</risdate><volume>12</volume><issue>2</issue><spage>1012</spage><epage>1021</epage><pages>1012-1021</pages><issn>1676-5680</issn><eissn>1676-5680</eissn><abstract>We examined microRNA-181b (miRNA) expression in prostate cancer tissues and its effect on the prostate cancer cell line PC-3. Tissues from 27 cases of prostate cancer and 30 samples of normal human prostate were collected by surgical removal. Total miRNA was extracted, and the relative expression of miR-181b was quantified using RT-PCR. miR-181b ASO was transfected into prostate cancer PC-3 cells. miR-181b expression in transfected and non-transfected cells was measured using RT-PCR. Changes in cell apoptosis were measured using flow cytometry. MTT and cell growth curve methods were used to assess the influence of miR-181b expression on cell proliferation. The changes in cell invasive ability in vitro were detected using the Transwell chamber method. miR-181b was up-regulated in the prostate cancer tissues compared with the normal prostate samples. It was down-regulated after miR-181b ASO transfection into the prostate cancer PC-3 cells. Down-regulation of miR-181b in the PC-3 cell induced apoptosis, inhibited proliferation, and depressed invasion of PC-3 cells in vitro. As miR-181b is over-expressed in prostate cancer, its down-regulation could have potential as gene therapy for prostate cancer by inducing apoptosis, inhibiting proliferation and depressing invasion by cancer cells.</abstract><cop>Brazil</cop><pmid>23613247</pmid><doi>10.4238/2013.April.2.17</doi><tpages>10</tpages></addata></record> |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | Apoptosis - genetics Cell Line, Tumor Cell Movement - genetics Cell Proliferation Gene Expression Gene Expression Regulation, Neoplastic Humans Male MicroRNAs - genetics MicroRNAs - metabolism Prostatic Neoplasms - genetics Prostatic Neoplasms - metabolism Transfection |
| title | MicroRNA-181b expression in prostate cancer tissues and its influence on the biological behavior of the prostate cancer cell line PC-3 |
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