Purification, Characterization, and Specificity Determination of a New Serine Protease Secreted by Penicillium waksmanii

The purpose of this work was to purify a protease from Penicillium waksmanii and to determine its biochemical characteristics and specificity. The extracellular protease isolated that was produced by P. waksmanii is a serine protease that is essential for the reproduction and growth of the fungus. T...

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Veröffentlicht in:	Applied biochemistry and biotechnology 2013, Vol.169 (1), p.201-214
Hauptverfasser:	Graminho, Eduardo Rezende, da Silva, Ronivaldo Rodrigues, de Freitas Cabral, Tatiana Pereira, Arantes, Eliane Candiani, da Rosa, Nathalia Gonsales, Juliano, Luiz, Okamoto, Debora Noma, de Oliveira, Lilian Caroline Gonçalves, Kondo, Marcia Yuri, Juliano, Maria Aparecida, Cabral, Hamilton
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Amino acids Biochemistry Biological and medical sciences Biotechnology Chemistry Chemistry and Materials Science Energy transfer Enzyme Stability Extracellular Space - chemistry Extracellular Space - enzymology Extracellular Space - genetics Fundamental and applied biological sciences. Psychology Fungal Proteins - chemistry Fungal Proteins - genetics Fungal Proteins - isolation & purification Fungal Proteins - metabolism Fungi Kinetics Molecular Sequence Data Penicillium Penicillium - chemistry Penicillium - enzymology Penicillium - genetics Penicillium chrysogenum Penicillium citrinum Proteases Protein Transport Serine Proteases - chemistry Serine Proteases - genetics Serine Proteases - isolation & purification Serine Proteases - metabolism Substrate Specificity Urea
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