Detection of Mycobacterium avium subsp. paratuberculosis in bulk tank milk by combined phage-PCR assay: Evidence that plaque number is a good predictor of MAP

Conventional culture and a rapid phage-PCR method were used to detect Mycobacterium avium subsp. paratuberculosis (MAP) in bulk tank milk (BTM) samples. Only two of 225 samples (0.9%) were found to contain MAP by culture whereas 50 (22%) MAP-positive samples were identified using the phage-PCR assay...

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Veröffentlicht in:	International journal of food microbiology 2013-06, Vol.164 (1), p.76-80
Hauptverfasser:	Botsaris, George, Liapi, Maria, Kakogiannis, Charalambos, Dodd, Christine E.R., Rees, Catherine E.D.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Biological and medical sciences Detection DNA, Bacterial - analysis Food industries Food microbiology Food Microbiology - methods Fundamental and applied biological sciences. Psychology General aspects Methods of analysis, processing and quality control, regulation, standards Milk Milk - microbiology Mycobacterium avium Mycobacterium avium subsp. paratuberculosis - genetics Mycobacterium avium subsp. paratuberculosis - isolation & purification Mycobacterium avium subsp. paratuberculosis - physiology Paratuberculosis Phage-PCR assay Polymerase Chain Reaction Reproducibility of Results Sensitivity and Specificity Viral Plaque Assay - standards
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