Analysis of Genetic Lineages and Their Correlation with Virulence Genes in Enterococcus faecalis Clinical Isolates from Root Canal and Systemic Infections
Abstract Introduction Enterococcus faecalis is a member of the mammalian gastrointestinal microbiota but has been considered a leading cause of hospital-acquired infections. In the oral cavity, it is commonly detected from root canals of teeth with failed endodontic treatment. However, little is kno...
Gespeichert in:
| Veröffentlicht in: | Journal of endodontics 2013-07, Vol.39 (7), p.858-864 |
|---|---|
| Hauptverfasser: | , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 864 |
|---|---|
| container_issue | 7 |
| container_start_page | 858 |
| container_title | Journal of endodontics |
| container_volume | 39 |
| creator | Penas, Pâmela P., MSc Mayer, Marcia P.A., PhD, MSc Gomes, Brenda P.F.A., PhD, MSc Endo, Marcos, MSc Pignatari, Antonio C.C., PhD, MSc Bauab, Karen C., MSc Pinheiro, Ericka T., PhD |
| description | Abstract Introduction Enterococcus faecalis is a member of the mammalian gastrointestinal microbiota but has been considered a leading cause of hospital-acquired infections. In the oral cavity, it is commonly detected from root canals of teeth with failed endodontic treatment. However, little is known about the virulence and genetic relatedness among E. faecalis isolates from different clinical sources. This study compared the presence of enterococcal virulence factors among root canal strains and clinical isolates from hospitalized patients to identify virulent clusters of E. faecalis. Methods Multilocus sequence typing analysis was used to determine genetic lineages of 40 E. faecalis clinical isolates from different sources. Virulence clusters were determined by evaluating capsule ( cps ) locus polymorphisms, pathogenicity island gene content, and antibiotic resistance genes by polymerase chain reaction. Results The clinical isolates from hospitalized patients formed a phylogenetically separate group and were mostly grouped in the clonal complex 2, which is a known virulent cluster of E. faecalis that has caused infection outbreaks globally. The clonal complex 2 group comprised capsule-producing strains harboring multiple antibiotic resistance and pathogenicity island genes. On the other hand, the endodontic isolates were more diverse and harbored few virulence and antibiotic resistance genes. In particular, although more closely related to isolates from hospitalized patients, capsule-producing E. faecalis strains from root canals did not carry more virulence/antibiotic genes than other endodontic isolates. Conclusions E. faecalis isolates from endodontic infections have a genetic and virulence profile different from pathogenic clusters of hospitalized patients' isolates, which is most likely due to niche specialization conferred mainly by variable regions in the genome. |
| doi_str_mv | 10.1016/j.joen.2013.01.009 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1371270842</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>1_s2_0_S0099239913001374</els_id><sourcerecordid>1371270842</sourcerecordid><originalsourceid>FETCH-LOGICAL-c411t-d11567365c303d82e572e98bbb5ff3de6d4fc42f08a2f629738122e57932190b3</originalsourceid><addsrcrecordid>eNp9Ustu1DAUtRAVnRZ-gAXykk2CH0mcSAipikoZaSSkPthajnNNHRK72AlofoWvrdMZWLDoylfWeeiecxF6S0lOCa0-DPngweWMUJ4TmhPSvEAbWos642VZvESb9NNkjDfNKTqLcSCECs7FK3TKuGgoK9kG_blwatxHG7E3-AoczFbjnXWgvkPEyvX49h5swK0PAUY1W-_wbzvf4282LCM4DU-siK3Dl26G4LXXeonYKNBqTLrtaJ1NI95GnwQS1AQ_4WvvZ9yq5P7kcrOPM0zJe-sM6NUmvkYnRo0R3hzfc3T3-fK2_ZLtvl5t24tdpgtK56yntKwEr0rNCe9rBqVg0NRd15XG8B6qvjC6YIbUipmKNYLXlK2ohjPakI6fo_cH3Yfgfy4QZznZqGEclQO_REm5oEyQumAJyg5QHXyMAYx8CHZSYS8pkWsncpBrJ3LtRBIqUwOJ9O6ov3QT9P8of0tIgI8HAKQtf1kIMmq7RtvbkLKQvbfP63_6j66Pkf-APcTBLyGlnPaQkUkib9arWI-C8nQQXBT8EUWns64</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1371270842</pqid></control><display><type>article</type><title>Analysis of Genetic Lineages and Their Correlation with Virulence Genes in Enterococcus faecalis Clinical Isolates from Root Canal and Systemic Infections</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><creator>Penas, Pâmela P., MSc ; Mayer, Marcia P.A., PhD, MSc ; Gomes, Brenda P.F.A., PhD, MSc ; Endo, Marcos, MSc ; Pignatari, Antonio C.C., PhD, MSc ; Bauab, Karen C., MSc ; Pinheiro, Ericka T., PhD</creator><creatorcontrib>Penas, Pâmela P., MSc ; Mayer, Marcia P.A., PhD, MSc ; Gomes, Brenda P.F.A., PhD, MSc ; Endo, Marcos, MSc ; Pignatari, Antonio C.C., PhD, MSc ; Bauab, Karen C., MSc ; Pinheiro, Ericka T., PhD</creatorcontrib><description>Abstract Introduction Enterococcus faecalis is a member of the mammalian gastrointestinal microbiota but has been considered a leading cause of hospital-acquired infections. In the oral cavity, it is commonly detected from root canals of teeth with failed endodontic treatment. However, little is known about the virulence and genetic relatedness among E. faecalis isolates from different clinical sources. This study compared the presence of enterococcal virulence factors among root canal strains and clinical isolates from hospitalized patients to identify virulent clusters of E. faecalis. Methods Multilocus sequence typing analysis was used to determine genetic lineages of 40 E. faecalis clinical isolates from different sources. Virulence clusters were determined by evaluating capsule ( cps ) locus polymorphisms, pathogenicity island gene content, and antibiotic resistance genes by polymerase chain reaction. Results The clinical isolates from hospitalized patients formed a phylogenetically separate group and were mostly grouped in the clonal complex 2, which is a known virulent cluster of E. faecalis that has caused infection outbreaks globally. The clonal complex 2 group comprised capsule-producing strains harboring multiple antibiotic resistance and pathogenicity island genes. On the other hand, the endodontic isolates were more diverse and harbored few virulence and antibiotic resistance genes. In particular, although more closely related to isolates from hospitalized patients, capsule-producing E. faecalis strains from root canals did not carry more virulence/antibiotic genes than other endodontic isolates. Conclusions E. faecalis isolates from endodontic infections have a genetic and virulence profile different from pathogenic clusters of hospitalized patients' isolates, which is most likely due to niche specialization conferred mainly by variable regions in the genome.</description><identifier>ISSN: 0099-2399</identifier><identifier>EISSN: 1878-3554</identifier><identifier>DOI: 10.1016/j.joen.2013.01.009</identifier><identifier>PMID: 23791252</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Antibiotic resistance ; Bacterial Capsules - genetics ; Bacterial Typing Techniques ; Chromosome Mapping ; Cross Infection - microbiology ; Dental Pulp Cavity - microbiology ; Dentistry ; Drug Resistance, Bacterial - genetics ; Endocrinology & Metabolism ; Enterococcus faecalis ; Enterococcus faecalis - classification ; Enterococcus faecalis - genetics ; Enterococcus faecalis - pathogenicity ; Genomic Islands - genetics ; genotypes ; Gram-Positive Bacterial Infections - microbiology ; Humans ; Multigene Family - genetics ; Multilocus Sequence Typing ; Polymorphism, Genetic - genetics ; root canals ; Tetracycline Resistance - genetics ; Tooth, Nonvital - microbiology ; Virulence - genetics ; virulence factors ; Virulence Factors - genetics</subject><ispartof>Journal of endodontics, 2013-07, Vol.39 (7), p.858-864</ispartof><rights>American Association of Endodontists</rights><rights>2013 American Association of Endodontists</rights><rights>Copyright © 2013 American Association of Endodontists. Published by Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c411t-d11567365c303d82e572e98bbb5ff3de6d4fc42f08a2f629738122e57932190b3</citedby><cites>FETCH-LOGICAL-c411t-d11567365c303d82e572e98bbb5ff3de6d4fc42f08a2f629738122e57932190b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0099239913001374$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23791252$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Penas, Pâmela P., MSc</creatorcontrib><creatorcontrib>Mayer, Marcia P.A., PhD, MSc</creatorcontrib><creatorcontrib>Gomes, Brenda P.F.A., PhD, MSc</creatorcontrib><creatorcontrib>Endo, Marcos, MSc</creatorcontrib><creatorcontrib>Pignatari, Antonio C.C., PhD, MSc</creatorcontrib><creatorcontrib>Bauab, Karen C., MSc</creatorcontrib><creatorcontrib>Pinheiro, Ericka T., PhD</creatorcontrib><title>Analysis of Genetic Lineages and Their Correlation with Virulence Genes in Enterococcus faecalis Clinical Isolates from Root Canal and Systemic Infections</title><title>Journal of endodontics</title><addtitle>J Endod</addtitle><description>Abstract Introduction Enterococcus faecalis is a member of the mammalian gastrointestinal microbiota but has been considered a leading cause of hospital-acquired infections. In the oral cavity, it is commonly detected from root canals of teeth with failed endodontic treatment. However, little is known about the virulence and genetic relatedness among E. faecalis isolates from different clinical sources. This study compared the presence of enterococcal virulence factors among root canal strains and clinical isolates from hospitalized patients to identify virulent clusters of E. faecalis. Methods Multilocus sequence typing analysis was used to determine genetic lineages of 40 E. faecalis clinical isolates from different sources. Virulence clusters were determined by evaluating capsule ( cps ) locus polymorphisms, pathogenicity island gene content, and antibiotic resistance genes by polymerase chain reaction. Results The clinical isolates from hospitalized patients formed a phylogenetically separate group and were mostly grouped in the clonal complex 2, which is a known virulent cluster of E. faecalis that has caused infection outbreaks globally. The clonal complex 2 group comprised capsule-producing strains harboring multiple antibiotic resistance and pathogenicity island genes. On the other hand, the endodontic isolates were more diverse and harbored few virulence and antibiotic resistance genes. In particular, although more closely related to isolates from hospitalized patients, capsule-producing E. faecalis strains from root canals did not carry more virulence/antibiotic genes than other endodontic isolates. Conclusions E. faecalis isolates from endodontic infections have a genetic and virulence profile different from pathogenic clusters of hospitalized patients' isolates, which is most likely due to niche specialization conferred mainly by variable regions in the genome.</description><subject>Antibiotic resistance</subject><subject>Bacterial Capsules - genetics</subject><subject>Bacterial Typing Techniques</subject><subject>Chromosome Mapping</subject><subject>Cross Infection - microbiology</subject><subject>Dental Pulp Cavity - microbiology</subject><subject>Dentistry</subject><subject>Drug Resistance, Bacterial - genetics</subject><subject>Endocrinology & Metabolism</subject><subject>Enterococcus faecalis</subject><subject>Enterococcus faecalis - classification</subject><subject>Enterococcus faecalis - genetics</subject><subject>Enterococcus faecalis - pathogenicity</subject><subject>Genomic Islands - genetics</subject><subject>genotypes</subject><subject>Gram-Positive Bacterial Infections - microbiology</subject><subject>Humans</subject><subject>Multigene Family - genetics</subject><subject>Multilocus Sequence Typing</subject><subject>Polymorphism, Genetic - genetics</subject><subject>root canals</subject><subject>Tetracycline Resistance - genetics</subject><subject>Tooth, Nonvital - microbiology</subject><subject>Virulence - genetics</subject><subject>virulence factors</subject><subject>Virulence Factors - genetics</subject><issn>0099-2399</issn><issn>1878-3554</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9Ustu1DAUtRAVnRZ-gAXykk2CH0mcSAipikoZaSSkPthajnNNHRK72AlofoWvrdMZWLDoylfWeeiecxF6S0lOCa0-DPngweWMUJ4TmhPSvEAbWos642VZvESb9NNkjDfNKTqLcSCECs7FK3TKuGgoK9kG_blwatxHG7E3-AoczFbjnXWgvkPEyvX49h5swK0PAUY1W-_wbzvf4282LCM4DU-siK3Dl26G4LXXeonYKNBqTLrtaJ1NI95GnwQS1AQ_4WvvZ9yq5P7kcrOPM0zJe-sM6NUmvkYnRo0R3hzfc3T3-fK2_ZLtvl5t24tdpgtK56yntKwEr0rNCe9rBqVg0NRd15XG8B6qvjC6YIbUipmKNYLXlK2ohjPakI6fo_cH3Yfgfy4QZznZqGEclQO_REm5oEyQumAJyg5QHXyMAYx8CHZSYS8pkWsncpBrJ3LtRBIqUwOJ9O6ov3QT9P8of0tIgI8HAKQtf1kIMmq7RtvbkLKQvbfP63_6j66Pkf-APcTBLyGlnPaQkUkib9arWI-C8nQQXBT8EUWns64</recordid><startdate>20130701</startdate><enddate>20130701</enddate><creator>Penas, Pâmela P., MSc</creator><creator>Mayer, Marcia P.A., PhD, MSc</creator><creator>Gomes, Brenda P.F.A., PhD, MSc</creator><creator>Endo, Marcos, MSc</creator><creator>Pignatari, Antonio C.C., PhD, MSc</creator><creator>Bauab, Karen C., MSc</creator><creator>Pinheiro, Ericka T., PhD</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20130701</creationdate><title>Analysis of Genetic Lineages and Their Correlation with Virulence Genes in Enterococcus faecalis Clinical Isolates from Root Canal and Systemic Infections</title><author>Penas, Pâmela P., MSc ; Mayer, Marcia P.A., PhD, MSc ; Gomes, Brenda P.F.A., PhD, MSc ; Endo, Marcos, MSc ; Pignatari, Antonio C.C., PhD, MSc ; Bauab, Karen C., MSc ; Pinheiro, Ericka T., PhD</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c411t-d11567365c303d82e572e98bbb5ff3de6d4fc42f08a2f629738122e57932190b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Antibiotic resistance</topic><topic>Bacterial Capsules - genetics</topic><topic>Bacterial Typing Techniques</topic><topic>Chromosome Mapping</topic><topic>Cross Infection - microbiology</topic><topic>Dental Pulp Cavity - microbiology</topic><topic>Dentistry</topic><topic>Drug Resistance, Bacterial - genetics</topic><topic>Endocrinology & Metabolism</topic><topic>Enterococcus faecalis</topic><topic>Enterococcus faecalis - classification</topic><topic>Enterococcus faecalis - genetics</topic><topic>Enterococcus faecalis - pathogenicity</topic><topic>Genomic Islands - genetics</topic><topic>genotypes</topic><topic>Gram-Positive Bacterial Infections - microbiology</topic><topic>Humans</topic><topic>Multigene Family - genetics</topic><topic>Multilocus Sequence Typing</topic><topic>Polymorphism, Genetic - genetics</topic><topic>root canals</topic><topic>Tetracycline Resistance - genetics</topic><topic>Tooth, Nonvital - microbiology</topic><topic>Virulence - genetics</topic><topic>virulence factors</topic><topic>Virulence Factors - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Penas, Pâmela P., MSc</creatorcontrib><creatorcontrib>Mayer, Marcia P.A., PhD, MSc</creatorcontrib><creatorcontrib>Gomes, Brenda P.F.A., PhD, MSc</creatorcontrib><creatorcontrib>Endo, Marcos, MSc</creatorcontrib><creatorcontrib>Pignatari, Antonio C.C., PhD, MSc</creatorcontrib><creatorcontrib>Bauab, Karen C., MSc</creatorcontrib><creatorcontrib>Pinheiro, Ericka T., PhD</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of endodontics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Penas, Pâmela P., MSc</au><au>Mayer, Marcia P.A., PhD, MSc</au><au>Gomes, Brenda P.F.A., PhD, MSc</au><au>Endo, Marcos, MSc</au><au>Pignatari, Antonio C.C., PhD, MSc</au><au>Bauab, Karen C., MSc</au><au>Pinheiro, Ericka T., PhD</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Analysis of Genetic Lineages and Their Correlation with Virulence Genes in Enterococcus faecalis Clinical Isolates from Root Canal and Systemic Infections</atitle><jtitle>Journal of endodontics</jtitle><addtitle>J Endod</addtitle><date>2013-07-01</date><risdate>2013</risdate><volume>39</volume><issue>7</issue><spage>858</spage><epage>864</epage><pages>858-864</pages><issn>0099-2399</issn><eissn>1878-3554</eissn><abstract>Abstract Introduction Enterococcus faecalis is a member of the mammalian gastrointestinal microbiota but has been considered a leading cause of hospital-acquired infections. In the oral cavity, it is commonly detected from root canals of teeth with failed endodontic treatment. However, little is known about the virulence and genetic relatedness among E. faecalis isolates from different clinical sources. This study compared the presence of enterococcal virulence factors among root canal strains and clinical isolates from hospitalized patients to identify virulent clusters of E. faecalis. Methods Multilocus sequence typing analysis was used to determine genetic lineages of 40 E. faecalis clinical isolates from different sources. Virulence clusters were determined by evaluating capsule ( cps ) locus polymorphisms, pathogenicity island gene content, and antibiotic resistance genes by polymerase chain reaction. Results The clinical isolates from hospitalized patients formed a phylogenetically separate group and were mostly grouped in the clonal complex 2, which is a known virulent cluster of E. faecalis that has caused infection outbreaks globally. The clonal complex 2 group comprised capsule-producing strains harboring multiple antibiotic resistance and pathogenicity island genes. On the other hand, the endodontic isolates were more diverse and harbored few virulence and antibiotic resistance genes. In particular, although more closely related to isolates from hospitalized patients, capsule-producing E. faecalis strains from root canals did not carry more virulence/antibiotic genes than other endodontic isolates. Conclusions E. faecalis isolates from endodontic infections have a genetic and virulence profile different from pathogenic clusters of hospitalized patients' isolates, which is most likely due to niche specialization conferred mainly by variable regions in the genome.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>23791252</pmid><doi>10.1016/j.joen.2013.01.009</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0099-2399 |
| ispartof | Journal of endodontics, 2013-07, Vol.39 (7), p.858-864 |
| issn | 0099-2399 1878-3554 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1371270842 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Antibiotic resistance Bacterial Capsules - genetics Bacterial Typing Techniques Chromosome Mapping Cross Infection - microbiology Dental Pulp Cavity - microbiology Dentistry Drug Resistance, Bacterial - genetics Endocrinology & Metabolism Enterococcus faecalis Enterococcus faecalis - classification Enterococcus faecalis - genetics Enterococcus faecalis - pathogenicity Genomic Islands - genetics genotypes Gram-Positive Bacterial Infections - microbiology Humans Multigene Family - genetics Multilocus Sequence Typing Polymorphism, Genetic - genetics root canals Tetracycline Resistance - genetics Tooth, Nonvital - microbiology Virulence - genetics virulence factors Virulence Factors - genetics |
| title | Analysis of Genetic Lineages and Their Correlation with Virulence Genes in Enterococcus faecalis Clinical Isolates from Root Canal and Systemic Infections |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-28T11%3A03%3A35IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Analysis%20of%20Genetic%20Lineages%20and%20Their%20Correlation%20with%20Virulence%20Genes%20in%20Enterococcus%20faecalis%20Clinical%20Isolates%20from%20Root%20Canal%20and%20Systemic%20Infections&rft.jtitle=Journal%20of%20endodontics&rft.au=Penas,%20P%C3%A2mela%20P.,%20MSc&rft.date=2013-07-01&rft.volume=39&rft.issue=7&rft.spage=858&rft.epage=864&rft.pages=858-864&rft.issn=0099-2399&rft.eissn=1878-3554&rft_id=info:doi/10.1016/j.joen.2013.01.009&rft_dat=%3Cproquest_cross%3E1371270842%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1371270842&rft_id=info:pmid/23791252&rft_els_id=1_s2_0_S0099239913001374&rfr_iscdi=true |