Lack of correlation between the size of HIV proviral DNA reservoir and the level of immune activation in HIV-infected patients with a sustained undetectable HIV viral load for 10 years
Abstract Background The persistence of HIV residual replication in patients with an undetectable plasma viral load (pVL) may limit immune recovery and facilitate inflammation-induced comorbidities. Objective The objective was to evaluate any correlation between immune restoration and intracellular [...
Gespeichert in:
| Veröffentlicht in: | Journal of clinical virology 2013-08, Vol.57 (4), p.351-355 |
|---|---|
| Hauptverfasser: | , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 355 |
|---|---|
| container_issue | 4 |
| container_start_page | 351 |
| container_title | Journal of clinical virology |
| container_volume | 57 |
| creator | Poizot-Martin, Isabelle Faucher, Olivia Obry-Roguet, Véronique Nicolino-Brunet, Corinne Ronot-Bregigeon, Sylvie Dignat-George, Françoise Tamalet, Catherine |
| description | Abstract Background The persistence of HIV residual replication in patients with an undetectable plasma viral load (pVL) may limit immune recovery and facilitate inflammation-induced comorbidities. Objective The objective was to evaluate any correlation between immune restoration and intracellular [IC] HIV-DNA in cART-treated patients with a sustained undetectable pVL. Study design This retrospective cross sectional study included 62 patients with a median duration of undetectable pVL of 10.3 years. IC HIV DNA in peripheral mononuclear blood cells (PBMCs) and T cell subsets were measured at the last visit. pVL, CD4+ and CD8+ T cell counts were retrospectively collected from the onset of long-term inhibition by antiretroviral treatment. The patients were separated into two groups: 27 non-blippers (sustained pVL < threshold value during all the visits) and 35 blippers (≥1 episodes of pVL > threshold but |
| doi_str_mv | 10.1016/j.jcv.2013.04.007 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1371270314</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>1_s2_0_S1386653213001285</els_id><sourcerecordid>1371270314</sourcerecordid><originalsourceid>FETCH-LOGICAL-c408t-cc3864da521ddb9fde12bd229123b44eaf3aa53ff0295c8d55bed0e6ff241ff3</originalsourceid><addsrcrecordid>eNp9ks1u1TAQhSMEoqXwAGyQl2wS_JO_KySkqgVa6QoWVGwtxx6rTh37YjupLk_G4-E0hQULVrbsb85ozpmieE1wRTBp343VKJeKYsIqXFcYd0-KU9J3rGx2bfc031nflm3D6EnxIsYRY9KwuntenFDWtnVHm9Pi117IO-Q1kj4EsCIZ79AA6R7AoXQLKJqfsP5fXX9Hh-AXE4RFl1_OUYAIYfEmIOHUA2phAbuyZppmB0jIZJZN0bhVoDROg0yg0CE_g0sR3Zt0iwSKc0zCuPwzOwUpQ2Kw8NB062i9UEj7gAhGRxAhviyeaWEjvHo8z4qbTx9vLq7K_dfP1xfn-1LWuE-llNmDWomGEqWGnVZA6KAo3RHKhroGoZkQDdMa010je9U0AygMrda0Jlqzs-LtJptn_zFDTHwyUYK1woGfIyesI7TDjNQZJRsqg48xgOaHYCYRjpxgvubFR57z4mteHNc855Vr3jzKz8ME6m_Fn4Ay8H4DIM-4GAg8yuycBGVCdokrb_4r_-GfammNM1LYOzhCHP0cXDaPEx4px_zbujDrvhCWd4X2DfsNnqC9wg</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1371270314</pqid></control><display><type>article</type><title>Lack of correlation between the size of HIV proviral DNA reservoir and the level of immune activation in HIV-infected patients with a sustained undetectable HIV viral load for 10 years</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><creator>Poizot-Martin, Isabelle ; Faucher, Olivia ; Obry-Roguet, Véronique ; Nicolino-Brunet, Corinne ; Ronot-Bregigeon, Sylvie ; Dignat-George, Françoise ; Tamalet, Catherine</creator><creatorcontrib>Poizot-Martin, Isabelle ; Faucher, Olivia ; Obry-Roguet, Véronique ; Nicolino-Brunet, Corinne ; Ronot-Bregigeon, Sylvie ; Dignat-George, Françoise ; Tamalet, Catherine</creatorcontrib><description>Abstract Background The persistence of HIV residual replication in patients with an undetectable plasma viral load (pVL) may limit immune recovery and facilitate inflammation-induced comorbidities. Objective The objective was to evaluate any correlation between immune restoration and intracellular [IC] HIV-DNA in cART-treated patients with a sustained undetectable pVL. Study design This retrospective cross sectional study included 62 patients with a median duration of undetectable pVL of 10.3 years. IC HIV DNA in peripheral mononuclear blood cells (PBMCs) and T cell subsets were measured at the last visit. pVL, CD4+ and CD8+ T cell counts were retrospectively collected from the onset of long-term inhibition by antiretroviral treatment. The patients were separated into two groups: 27 non-blippers (sustained pVL < threshold value during all the visits) and 35 blippers (≥1 episodes of pVL > threshold but <1000 copies/ml). The median pVL in blippers was 115 copies/ml. Results The median IC HIV DNA rate was 34 copies/106 PBMCs (71% ≥20 copies/106 PBMCs) with no significant difference between the groups. The proportion of CD8+ CD38+ and CD8+ DR+ T cells was higher in blipper patients, but the difference was only significant for the CD8+ DR+ marker ( p = 0.036). No correlation was found between markers of immune activation on CD4+ and CD8+ T cells and the IC HIV-DNA level. Conclusion No relation was found between the size of HIV reservoirs and immune activation in patients with sustained undetectable pVL. Mechanisms of immune activation have to be better understood in order to define specific therapeutic interventions.</description><identifier>ISSN: 1386-6532</identifier><identifier>EISSN: 1873-5967</identifier><identifier>DOI: 10.1016/j.jcv.2013.04.007</identifier><identifier>PMID: 23664725</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Adult ; Allergy and Immunology ; Anti-HIV Agents - immunology ; Anti-HIV Agents - therapeutic use ; Antiretroviral Therapy, Highly Active - methods ; CD4-Positive T-Lymphocytes - drug effects ; CD4-Positive T-Lymphocytes - immunology ; CD4-Positive T-Lymphocytes - virology ; CD8-Positive T-Lymphocytes - drug effects ; CD8-Positive T-Lymphocytes - immunology ; CD8-Positive T-Lymphocytes - virology ; Cross-Sectional Studies ; DNA, Viral - genetics ; DNA, Viral - immunology ; Female ; HIV - genetics ; HIV - immunology ; HIV DNA-proviral ; HIV Infections - blood ; HIV Infections - drug therapy ; HIV Infections - immunology ; HIV Infections - virology ; HIV reservoir ; HIV-1 ; Humans ; Immune activation markers ; Infectious Disease ; Leukocytes, Mononuclear - immunology ; Leukocytes, Mononuclear - virology ; Lymphocyte Activation - immunology ; Male ; Middle Aged ; Retrospective Studies ; RNA, Viral - blood ; RNA, Viral - immunology ; T-Lymphocyte Subsets - drug effects ; T-Lymphocyte Subsets - immunology ; T-Lymphocyte Subsets - virology ; Viral Load - immunology</subject><ispartof>Journal of clinical virology, 2013-08, Vol.57 (4), p.351-355</ispartof><rights>Elsevier B.V.</rights><rights>2013 Elsevier B.V.</rights><rights>Copyright © 2013 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c408t-cc3864da521ddb9fde12bd229123b44eaf3aa53ff0295c8d55bed0e6ff241ff3</citedby><cites>FETCH-LOGICAL-c408t-cc3864da521ddb9fde12bd229123b44eaf3aa53ff0295c8d55bed0e6ff241ff3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S1386653213001285$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23664725$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Poizot-Martin, Isabelle</creatorcontrib><creatorcontrib>Faucher, Olivia</creatorcontrib><creatorcontrib>Obry-Roguet, Véronique</creatorcontrib><creatorcontrib>Nicolino-Brunet, Corinne</creatorcontrib><creatorcontrib>Ronot-Bregigeon, Sylvie</creatorcontrib><creatorcontrib>Dignat-George, Françoise</creatorcontrib><creatorcontrib>Tamalet, Catherine</creatorcontrib><title>Lack of correlation between the size of HIV proviral DNA reservoir and the level of immune activation in HIV-infected patients with a sustained undetectable HIV viral load for 10 years</title><title>Journal of clinical virology</title><addtitle>J Clin Virol</addtitle><description>Abstract Background The persistence of HIV residual replication in patients with an undetectable plasma viral load (pVL) may limit immune recovery and facilitate inflammation-induced comorbidities. Objective The objective was to evaluate any correlation between immune restoration and intracellular [IC] HIV-DNA in cART-treated patients with a sustained undetectable pVL. Study design This retrospective cross sectional study included 62 patients with a median duration of undetectable pVL of 10.3 years. IC HIV DNA in peripheral mononuclear blood cells (PBMCs) and T cell subsets were measured at the last visit. pVL, CD4+ and CD8+ T cell counts were retrospectively collected from the onset of long-term inhibition by antiretroviral treatment. The patients were separated into two groups: 27 non-blippers (sustained pVL < threshold value during all the visits) and 35 blippers (≥1 episodes of pVL > threshold but <1000 copies/ml). The median pVL in blippers was 115 copies/ml. Results The median IC HIV DNA rate was 34 copies/106 PBMCs (71% ≥20 copies/106 PBMCs) with no significant difference between the groups. The proportion of CD8+ CD38+ and CD8+ DR+ T cells was higher in blipper patients, but the difference was only significant for the CD8+ DR+ marker ( p = 0.036). No correlation was found between markers of immune activation on CD4+ and CD8+ T cells and the IC HIV-DNA level. Conclusion No relation was found between the size of HIV reservoirs and immune activation in patients with sustained undetectable pVL. Mechanisms of immune activation have to be better understood in order to define specific therapeutic interventions.</description><subject>Adult</subject><subject>Allergy and Immunology</subject><subject>Anti-HIV Agents - immunology</subject><subject>Anti-HIV Agents - therapeutic use</subject><subject>Antiretroviral Therapy, Highly Active - methods</subject><subject>CD4-Positive T-Lymphocytes - drug effects</subject><subject>CD4-Positive T-Lymphocytes - immunology</subject><subject>CD4-Positive T-Lymphocytes - virology</subject><subject>CD8-Positive T-Lymphocytes - drug effects</subject><subject>CD8-Positive T-Lymphocytes - immunology</subject><subject>CD8-Positive T-Lymphocytes - virology</subject><subject>Cross-Sectional Studies</subject><subject>DNA, Viral - genetics</subject><subject>DNA, Viral - immunology</subject><subject>Female</subject><subject>HIV - genetics</subject><subject>HIV - immunology</subject><subject>HIV DNA-proviral</subject><subject>HIV Infections - blood</subject><subject>HIV Infections - drug therapy</subject><subject>HIV Infections - immunology</subject><subject>HIV Infections - virology</subject><subject>HIV reservoir</subject><subject>HIV-1</subject><subject>Humans</subject><subject>Immune activation markers</subject><subject>Infectious Disease</subject><subject>Leukocytes, Mononuclear - immunology</subject><subject>Leukocytes, Mononuclear - virology</subject><subject>Lymphocyte Activation - immunology</subject><subject>Male</subject><subject>Middle Aged</subject><subject>Retrospective Studies</subject><subject>RNA, Viral - blood</subject><subject>RNA, Viral - immunology</subject><subject>T-Lymphocyte Subsets - drug effects</subject><subject>T-Lymphocyte Subsets - immunology</subject><subject>T-Lymphocyte Subsets - virology</subject><subject>Viral Load - immunology</subject><issn>1386-6532</issn><issn>1873-5967</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9ks1u1TAQhSMEoqXwAGyQl2wS_JO_KySkqgVa6QoWVGwtxx6rTh37YjupLk_G4-E0hQULVrbsb85ozpmieE1wRTBp343VKJeKYsIqXFcYd0-KU9J3rGx2bfc031nflm3D6EnxIsYRY9KwuntenFDWtnVHm9Pi117IO-Q1kj4EsCIZ79AA6R7AoXQLKJqfsP5fXX9Hh-AXE4RFl1_OUYAIYfEmIOHUA2phAbuyZppmB0jIZJZN0bhVoDROg0yg0CE_g0sR3Zt0iwSKc0zCuPwzOwUpQ2Kw8NB062i9UEj7gAhGRxAhviyeaWEjvHo8z4qbTx9vLq7K_dfP1xfn-1LWuE-llNmDWomGEqWGnVZA6KAo3RHKhroGoZkQDdMa010je9U0AygMrda0Jlqzs-LtJptn_zFDTHwyUYK1woGfIyesI7TDjNQZJRsqg48xgOaHYCYRjpxgvubFR57z4mteHNc855Vr3jzKz8ME6m_Fn4Ay8H4DIM-4GAg8yuycBGVCdokrb_4r_-GfammNM1LYOzhCHP0cXDaPEx4px_zbujDrvhCWd4X2DfsNnqC9wg</recordid><startdate>20130801</startdate><enddate>20130801</enddate><creator>Poizot-Martin, Isabelle</creator><creator>Faucher, Olivia</creator><creator>Obry-Roguet, Véronique</creator><creator>Nicolino-Brunet, Corinne</creator><creator>Ronot-Bregigeon, Sylvie</creator><creator>Dignat-George, Françoise</creator><creator>Tamalet, Catherine</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20130801</creationdate><title>Lack of correlation between the size of HIV proviral DNA reservoir and the level of immune activation in HIV-infected patients with a sustained undetectable HIV viral load for 10 years</title><author>Poizot-Martin, Isabelle ; Faucher, Olivia ; Obry-Roguet, Véronique ; Nicolino-Brunet, Corinne ; Ronot-Bregigeon, Sylvie ; Dignat-George, Françoise ; Tamalet, Catherine</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c408t-cc3864da521ddb9fde12bd229123b44eaf3aa53ff0295c8d55bed0e6ff241ff3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Adult</topic><topic>Allergy and Immunology</topic><topic>Anti-HIV Agents - immunology</topic><topic>Anti-HIV Agents - therapeutic use</topic><topic>Antiretroviral Therapy, Highly Active - methods</topic><topic>CD4-Positive T-Lymphocytes - drug effects</topic><topic>CD4-Positive T-Lymphocytes - immunology</topic><topic>CD4-Positive T-Lymphocytes - virology</topic><topic>CD8-Positive T-Lymphocytes - drug effects</topic><topic>CD8-Positive T-Lymphocytes - immunology</topic><topic>CD8-Positive T-Lymphocytes - virology</topic><topic>Cross-Sectional Studies</topic><topic>DNA, Viral - genetics</topic><topic>DNA, Viral - immunology</topic><topic>Female</topic><topic>HIV - genetics</topic><topic>HIV - immunology</topic><topic>HIV DNA-proviral</topic><topic>HIV Infections - blood</topic><topic>HIV Infections - drug therapy</topic><topic>HIV Infections - immunology</topic><topic>HIV Infections - virology</topic><topic>HIV reservoir</topic><topic>HIV-1</topic><topic>Humans</topic><topic>Immune activation markers</topic><topic>Infectious Disease</topic><topic>Leukocytes, Mononuclear - immunology</topic><topic>Leukocytes, Mononuclear - virology</topic><topic>Lymphocyte Activation - immunology</topic><topic>Male</topic><topic>Middle Aged</topic><topic>Retrospective Studies</topic><topic>RNA, Viral - blood</topic><topic>RNA, Viral - immunology</topic><topic>T-Lymphocyte Subsets - drug effects</topic><topic>T-Lymphocyte Subsets - immunology</topic><topic>T-Lymphocyte Subsets - virology</topic><topic>Viral Load - immunology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Poizot-Martin, Isabelle</creatorcontrib><creatorcontrib>Faucher, Olivia</creatorcontrib><creatorcontrib>Obry-Roguet, Véronique</creatorcontrib><creatorcontrib>Nicolino-Brunet, Corinne</creatorcontrib><creatorcontrib>Ronot-Bregigeon, Sylvie</creatorcontrib><creatorcontrib>Dignat-George, Françoise</creatorcontrib><creatorcontrib>Tamalet, Catherine</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of clinical virology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Poizot-Martin, Isabelle</au><au>Faucher, Olivia</au><au>Obry-Roguet, Véronique</au><au>Nicolino-Brunet, Corinne</au><au>Ronot-Bregigeon, Sylvie</au><au>Dignat-George, Françoise</au><au>Tamalet, Catherine</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Lack of correlation between the size of HIV proviral DNA reservoir and the level of immune activation in HIV-infected patients with a sustained undetectable HIV viral load for 10 years</atitle><jtitle>Journal of clinical virology</jtitle><addtitle>J Clin Virol</addtitle><date>2013-08-01</date><risdate>2013</risdate><volume>57</volume><issue>4</issue><spage>351</spage><epage>355</epage><pages>351-355</pages><issn>1386-6532</issn><eissn>1873-5967</eissn><abstract>Abstract Background The persistence of HIV residual replication in patients with an undetectable plasma viral load (pVL) may limit immune recovery and facilitate inflammation-induced comorbidities. Objective The objective was to evaluate any correlation between immune restoration and intracellular [IC] HIV-DNA in cART-treated patients with a sustained undetectable pVL. Study design This retrospective cross sectional study included 62 patients with a median duration of undetectable pVL of 10.3 years. IC HIV DNA in peripheral mononuclear blood cells (PBMCs) and T cell subsets were measured at the last visit. pVL, CD4+ and CD8+ T cell counts were retrospectively collected from the onset of long-term inhibition by antiretroviral treatment. The patients were separated into two groups: 27 non-blippers (sustained pVL < threshold value during all the visits) and 35 blippers (≥1 episodes of pVL > threshold but <1000 copies/ml). The median pVL in blippers was 115 copies/ml. Results The median IC HIV DNA rate was 34 copies/106 PBMCs (71% ≥20 copies/106 PBMCs) with no significant difference between the groups. The proportion of CD8+ CD38+ and CD8+ DR+ T cells was higher in blipper patients, but the difference was only significant for the CD8+ DR+ marker ( p = 0.036). No correlation was found between markers of immune activation on CD4+ and CD8+ T cells and the IC HIV-DNA level. Conclusion No relation was found between the size of HIV reservoirs and immune activation in patients with sustained undetectable pVL. Mechanisms of immune activation have to be better understood in order to define specific therapeutic interventions.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>23664725</pmid><doi>10.1016/j.jcv.2013.04.007</doi><tpages>5</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1386-6532 |
| ispartof | Journal of clinical virology, 2013-08, Vol.57 (4), p.351-355 |
| issn | 1386-6532 1873-5967 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1371270314 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Adult Allergy and Immunology Anti-HIV Agents - immunology Anti-HIV Agents - therapeutic use Antiretroviral Therapy, Highly Active - methods CD4-Positive T-Lymphocytes - drug effects CD4-Positive T-Lymphocytes - immunology CD4-Positive T-Lymphocytes - virology CD8-Positive T-Lymphocytes - drug effects CD8-Positive T-Lymphocytes - immunology CD8-Positive T-Lymphocytes - virology Cross-Sectional Studies DNA, Viral - genetics DNA, Viral - immunology Female HIV - genetics HIV - immunology HIV DNA-proviral HIV Infections - blood HIV Infections - drug therapy HIV Infections - immunology HIV Infections - virology HIV reservoir HIV-1 Humans Immune activation markers Infectious Disease Leukocytes, Mononuclear - immunology Leukocytes, Mononuclear - virology Lymphocyte Activation - immunology Male Middle Aged Retrospective Studies RNA, Viral - blood RNA, Viral - immunology T-Lymphocyte Subsets - drug effects T-Lymphocyte Subsets - immunology T-Lymphocyte Subsets - virology Viral Load - immunology |
| title | Lack of correlation between the size of HIV proviral DNA reservoir and the level of immune activation in HIV-infected patients with a sustained undetectable HIV viral load for 10 years |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-06T01%3A03%3A18IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Lack%20of%20correlation%20between%20the%20size%20of%20HIV%20proviral%20DNA%20reservoir%20and%20the%20level%20of%20immune%20activation%20in%20HIV-infected%20patients%20with%20a%20sustained%20undetectable%20HIV%20viral%20load%20for%2010%20years&rft.jtitle=Journal%20of%20clinical%20virology&rft.au=Poizot-Martin,%20Isabelle&rft.date=2013-08-01&rft.volume=57&rft.issue=4&rft.spage=351&rft.epage=355&rft.pages=351-355&rft.issn=1386-6532&rft.eissn=1873-5967&rft_id=info:doi/10.1016/j.jcv.2013.04.007&rft_dat=%3Cproquest_cross%3E1371270314%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1371270314&rft_id=info:pmid/23664725&rft_els_id=1_s2_0_S1386653213001285&rfr_iscdi=true |