Cloning and characterization of bottlenose dolphin (Tursiops truncatus) interleukin-10
The function of cytokines in cetaceans has so far only been determined for the proinflammatory cytokines. In this study, we cloned bottlenose dolphin (Tursiops truncatus) interleukin-10 (IL-10) cDNA from concanavalin A (Con A)-stimulated peripheral blood mononuclear cells (PBMC), and investigated th...
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| Veröffentlicht in: | Veterinary immunology and immunopathology 2013-07, Vol.154 (1-2), p.62-67 |
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| container_title | Veterinary immunology and immunopathology |
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| creator | Segawa, Takao Karatani, Nana Itou, Takuya Suzuki, Miwa Sakai, Takeo |
| description | The function of cytokines in cetaceans has so far only been determined for the proinflammatory cytokines. In this study, we cloned bottlenose dolphin (Tursiops truncatus) interleukin-10 (IL-10) cDNA from concanavalin A (Con A)-stimulated peripheral blood mononuclear cells (PBMC), and investigated the mRNA expression levels in various tissues and the bioactivity of recombinant dolphin (rd) IL-10. The gene encodes a polypeptide of 178 amino acids which encompasses the mature protein sequence of 158 amino acids. Quantitative expression analysis of dolphin IL-10 revealed that the highest mRNA levels are found in the spleen. To assess its function, rdIL-10 was produced in human embryonic kidney 293 cells and its bioactivity was demonstrated through IL-10-induced inhibition of proinflammatory cytokine mRNA expression IFN-γ, TNF-α, and IL-2 of Con A-stimulated PBMC. These results indicated that the structure and function of bottlenose dolphin IL-10 is similar to that of other animals. This is the first report of the characterization of an anti-inflammatory cytokine in cetaceans. |
| doi_str_mv | 10.1016/j.vetimm.2013.04.009 |
| format | Article |
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In this study, we cloned bottlenose dolphin (Tursiops truncatus) interleukin-10 (IL-10) cDNA from concanavalin A (Con A)-stimulated peripheral blood mononuclear cells (PBMC), and investigated the mRNA expression levels in various tissues and the bioactivity of recombinant dolphin (rd) IL-10. The gene encodes a polypeptide of 178 amino acids which encompasses the mature protein sequence of 158 amino acids. Quantitative expression analysis of dolphin IL-10 revealed that the highest mRNA levels are found in the spleen. To assess its function, rdIL-10 was produced in human embryonic kidney 293 cells and its bioactivity was demonstrated through IL-10-induced inhibition of proinflammatory cytokine mRNA expression IFN-γ, TNF-α, and IL-2 of Con A-stimulated PBMC. These results indicated that the structure and function of bottlenose dolphin IL-10 is similar to that of other animals. This is the first report of the characterization of an anti-inflammatory cytokine in cetaceans.</description><identifier>ISSN: 0165-2427</identifier><identifier>EISSN: 1873-2534</identifier><identifier>DOI: 10.1016/j.vetimm.2013.04.009</identifier><identifier>PMID: 23668955</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Amino Acid Sequence ; Animals ; Bottle-Nosed Dolphin - genetics ; Bottle-Nosed Dolphin - metabolism ; Cloning, Molecular ; DNA, Complementary ; Dolphin ; Interleukin-10 ; Interleukin-10 - genetics ; Interleukin-10 - metabolism ; Molecular Sequence Data ; Phylogeny ; RNA, Messenger - genetics ; RNA, Messenger - metabolism</subject><ispartof>Veterinary immunology and immunopathology, 2013-07, Vol.154 (1-2), p.62-67</ispartof><rights>2013 Elsevier B.V.</rights><rights>Copyright © 2013 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c428t-e9eff6818e0cc2157446f465da3af07bef90e1837cbec46e4c9b86e4314e08d93</citedby><cites>FETCH-LOGICAL-c428t-e9eff6818e0cc2157446f465da3af07bef90e1837cbec46e4c9b86e4314e08d93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.vetimm.2013.04.009$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3548,27923,27924,45994</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23668955$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Segawa, Takao</creatorcontrib><creatorcontrib>Karatani, Nana</creatorcontrib><creatorcontrib>Itou, Takuya</creatorcontrib><creatorcontrib>Suzuki, Miwa</creatorcontrib><creatorcontrib>Sakai, Takeo</creatorcontrib><title>Cloning and characterization of bottlenose dolphin (Tursiops truncatus) interleukin-10</title><title>Veterinary immunology and immunopathology</title><addtitle>Vet Immunol Immunopathol</addtitle><description>The function of cytokines in cetaceans has so far only been determined for the proinflammatory cytokines. In this study, we cloned bottlenose dolphin (Tursiops truncatus) interleukin-10 (IL-10) cDNA from concanavalin A (Con A)-stimulated peripheral blood mononuclear cells (PBMC), and investigated the mRNA expression levels in various tissues and the bioactivity of recombinant dolphin (rd) IL-10. The gene encodes a polypeptide of 178 amino acids which encompasses the mature protein sequence of 158 amino acids. Quantitative expression analysis of dolphin IL-10 revealed that the highest mRNA levels are found in the spleen. To assess its function, rdIL-10 was produced in human embryonic kidney 293 cells and its bioactivity was demonstrated through IL-10-induced inhibition of proinflammatory cytokine mRNA expression IFN-γ, TNF-α, and IL-2 of Con A-stimulated PBMC. These results indicated that the structure and function of bottlenose dolphin IL-10 is similar to that of other animals. This is the first report of the characterization of an anti-inflammatory cytokine in cetaceans.</description><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Bottle-Nosed Dolphin - genetics</subject><subject>Bottle-Nosed Dolphin - metabolism</subject><subject>Cloning, Molecular</subject><subject>DNA, Complementary</subject><subject>Dolphin</subject><subject>Interleukin-10</subject><subject>Interleukin-10 - genetics</subject><subject>Interleukin-10 - metabolism</subject><subject>Molecular Sequence Data</subject><subject>Phylogeny</subject><subject>RNA, Messenger - genetics</subject><subject>RNA, Messenger - metabolism</subject><issn>0165-2427</issn><issn>1873-2534</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1LxDAURYMoOo7-A5EuddH60qRtuhFk8AsEN6PbkKavmrFNxiQd0F9vZdSlq7s5913eIeSEQkaBlherbIPRDEOWA2UZ8Ayg3iEzKiqW5gXju2Q2YUWa87w6IIchrACgqIXYJwc5K0tRF8WMPC96Z419SZRtE_2qvNIRvflU0TibuC5pXIw9WhcwaV2_fjU2OVuOPhi3Dkn0o9UqjuE8MXbq9Ti-GZtSOCJ7neoDHv_knDzdXC8Xd-nD4-394uoh1TwXMcUau64UVCBondOi4rzseFm0iqkOqga7GpAKVukGNS-R67oRUzDKEURbszk5295de_c-YohyMEFj3yuLbgySsgpKVudFNaF8i2rvQvDYybU3g_IfkoL8NipXcmtUfhuVwOVkdKqd_iyMzYDtX-lX4QRcbgGc_twY9DJog1ZjazzqKFtn_l_4Ai3yipM</recordid><startdate>20130715</startdate><enddate>20130715</enddate><creator>Segawa, Takao</creator><creator>Karatani, Nana</creator><creator>Itou, Takuya</creator><creator>Suzuki, Miwa</creator><creator>Sakai, Takeo</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20130715</creationdate><title>Cloning and characterization of bottlenose dolphin (Tursiops truncatus) interleukin-10</title><author>Segawa, Takao ; Karatani, Nana ; Itou, Takuya ; Suzuki, Miwa ; Sakai, Takeo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c428t-e9eff6818e0cc2157446f465da3af07bef90e1837cbec46e4c9b86e4314e08d93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Bottle-Nosed Dolphin - genetics</topic><topic>Bottle-Nosed Dolphin - metabolism</topic><topic>Cloning, Molecular</topic><topic>DNA, Complementary</topic><topic>Dolphin</topic><topic>Interleukin-10</topic><topic>Interleukin-10 - genetics</topic><topic>Interleukin-10 - metabolism</topic><topic>Molecular Sequence Data</topic><topic>Phylogeny</topic><topic>RNA, Messenger - genetics</topic><topic>RNA, Messenger - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Segawa, Takao</creatorcontrib><creatorcontrib>Karatani, Nana</creatorcontrib><creatorcontrib>Itou, Takuya</creatorcontrib><creatorcontrib>Suzuki, Miwa</creatorcontrib><creatorcontrib>Sakai, Takeo</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Veterinary immunology and immunopathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Segawa, Takao</au><au>Karatani, Nana</au><au>Itou, Takuya</au><au>Suzuki, Miwa</au><au>Sakai, Takeo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cloning and characterization of bottlenose dolphin (Tursiops truncatus) interleukin-10</atitle><jtitle>Veterinary immunology and immunopathology</jtitle><addtitle>Vet Immunol Immunopathol</addtitle><date>2013-07-15</date><risdate>2013</risdate><volume>154</volume><issue>1-2</issue><spage>62</spage><epage>67</epage><pages>62-67</pages><issn>0165-2427</issn><eissn>1873-2534</eissn><abstract>The function of cytokines in cetaceans has so far only been determined for the proinflammatory cytokines. In this study, we cloned bottlenose dolphin (Tursiops truncatus) interleukin-10 (IL-10) cDNA from concanavalin A (Con A)-stimulated peripheral blood mononuclear cells (PBMC), and investigated the mRNA expression levels in various tissues and the bioactivity of recombinant dolphin (rd) IL-10. The gene encodes a polypeptide of 178 amino acids which encompasses the mature protein sequence of 158 amino acids. Quantitative expression analysis of dolphin IL-10 revealed that the highest mRNA levels are found in the spleen. To assess its function, rdIL-10 was produced in human embryonic kidney 293 cells and its bioactivity was demonstrated through IL-10-induced inhibition of proinflammatory cytokine mRNA expression IFN-γ, TNF-α, and IL-2 of Con A-stimulated PBMC. These results indicated that the structure and function of bottlenose dolphin IL-10 is similar to that of other animals. 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| ispartof | Veterinary immunology and immunopathology, 2013-07, Vol.154 (1-2), p.62-67 |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Amino Acid Sequence Animals Bottle-Nosed Dolphin - genetics Bottle-Nosed Dolphin - metabolism Cloning, Molecular DNA, Complementary Dolphin Interleukin-10 Interleukin-10 - genetics Interleukin-10 - metabolism Molecular Sequence Data Phylogeny RNA, Messenger - genetics RNA, Messenger - metabolism |
| title | Cloning and characterization of bottlenose dolphin (Tursiops truncatus) interleukin-10 |
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