SEROLOGIC, MOLECULAR, AND PATHOLOGIC SURVEY OF TOXOPLASMA GONDII INFECTION IN FREE-RANGING RED FOXES (VULPES VULPES) IN CENTRAL ITALY
We tested 191 sera of red foxes (Vulpes vulpes; 78 females and 113 males) for antibodies to Toxoplasma gondii using an indirect immunofluorescent test. Tissue samples of myocardium, lymph nodes, and brains from antibody-positive animals were tested for T. gondii DNA using specific PCR and processed...
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| Veröffentlicht in: | Journal of wildlife diseases 2013-07, Vol.49 (3), p.545-551 |
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| container_title | Journal of wildlife diseases |
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| creator | Verin, Ranieri Mugnaini, Linda Nardoni, Simona Papini, Roberto Amerigo Ariti, Gaetano Poli, Alessandro Mancianti, Francesca |
| description | We tested 191 sera of red foxes (Vulpes vulpes; 78 females and 113 males) for antibodies to Toxoplasma gondii using an indirect immunofluorescent test. Tissue samples of myocardium, lymph nodes, and brains from antibody-positive animals were tested for T. gondii DNA using specific PCR and processed for histopathology and immunohistochemistry (IHC) to detect T. gondii antigen. Of 192 (53.4%) antibody-positive animals, eight were positive by PCR on myocardium and one on brain. All DNA extracts were genotyped. Histopathology showed lesions characteristic of protozoan encephalitis; IHC did not show T. gondii antigen in examined tissues. The high antibody prevalence found in our study, which is the first in Italy, and the occurrence of polymorphic strains (combination of different type I and III alleles) divergent from typical T. gondii strains, suggests red foxes may be a sentinel of T. gondii in the environment. |
| doi_str_mv | 10.7589/2011-07-204 |
| format | Article |
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Tissue samples of myocardium, lymph nodes, and brains from antibody-positive animals were tested for T. gondii DNA using specific PCR and processed for histopathology and immunohistochemistry (IHC) to detect T. gondii antigen. Of 192 (53.4%) antibody-positive animals, eight were positive by PCR on myocardium and one on brain. All DNA extracts were genotyped. Histopathology showed lesions characteristic of protozoan encephalitis; IHC did not show T. gondii antigen in examined tissues. The high antibody prevalence found in our study, which is the first in Italy, and the occurrence of polymorphic strains (combination of different type I and III alleles) divergent from typical T. gondii strains, suggests red foxes may be a sentinel of T. gondii in the environment.</description><identifier>ISSN: 0090-3558</identifier><identifier>EISSN: 1943-3700</identifier><identifier>DOI: 10.7589/2011-07-204</identifier><identifier>PMID: 23778603</identifier><language>eng</language><publisher>United States: Wildlife Disease Association</publisher><subject>Animals ; Animals, Wild - parasitology ; Antibodies, Protozoan - blood ; Disease Reservoirs - parasitology ; Disease Reservoirs - veterinary ; Female ; Foxes - parasitology ; Genotyping ; histopathology ; IFAT ; immunohistochemistry ; Immunohistochemistry - veterinary ; Italy ; Male ; PATHOLOGY ; PCR ; red fox ; Sentinel Surveillance - veterinary ; Toxoplasma - immunology ; Toxoplasma - isolation & purification ; Toxoplasma gondii ; Toxoplasmosis, Animal - blood ; Toxoplasmosis, Animal - epidemiology ; Toxoplasmosis, Animal - pathology ; Vulpes vulpes</subject><ispartof>Journal of wildlife diseases, 2013-07, Vol.49 (3), p.545-551</ispartof><rights>2013 Wildlife Disease Association</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-b323t-1fc38a5b13ae1725d5db01c484a88d6bb637a7d9ee0e8ae19ffbe90b079640c93</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27923,27924</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23778603$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Verin, Ranieri</creatorcontrib><creatorcontrib>Mugnaini, Linda</creatorcontrib><creatorcontrib>Nardoni, Simona</creatorcontrib><creatorcontrib>Papini, Roberto Amerigo</creatorcontrib><creatorcontrib>Ariti, Gaetano</creatorcontrib><creatorcontrib>Poli, Alessandro</creatorcontrib><creatorcontrib>Mancianti, Francesca</creatorcontrib><title>SEROLOGIC, MOLECULAR, AND PATHOLOGIC SURVEY OF TOXOPLASMA GONDII INFECTION IN FREE-RANGING RED FOXES (VULPES VULPES) IN CENTRAL ITALY</title><title>Journal of wildlife diseases</title><addtitle>J Wildl Dis</addtitle><description>We tested 191 sera of red foxes (Vulpes vulpes; 78 females and 113 males) for antibodies to Toxoplasma gondii using an indirect immunofluorescent test. Tissue samples of myocardium, lymph nodes, and brains from antibody-positive animals were tested for T. gondii DNA using specific PCR and processed for histopathology and immunohistochemistry (IHC) to detect T. gondii antigen. Of 192 (53.4%) antibody-positive animals, eight were positive by PCR on myocardium and one on brain. All DNA extracts were genotyped. Histopathology showed lesions characteristic of protozoan encephalitis; IHC did not show T. gondii antigen in examined tissues. The high antibody prevalence found in our study, which is the first in Italy, and the occurrence of polymorphic strains (combination of different type I and III alleles) divergent from typical T. gondii strains, suggests red foxes may be a sentinel of T. gondii in the environment.</description><subject>Animals</subject><subject>Animals, Wild - parasitology</subject><subject>Antibodies, Protozoan - blood</subject><subject>Disease Reservoirs - parasitology</subject><subject>Disease Reservoirs - veterinary</subject><subject>Female</subject><subject>Foxes - parasitology</subject><subject>Genotyping</subject><subject>histopathology</subject><subject>IFAT</subject><subject>immunohistochemistry</subject><subject>Immunohistochemistry - veterinary</subject><subject>Italy</subject><subject>Male</subject><subject>PATHOLOGY</subject><subject>PCR</subject><subject>red fox</subject><subject>Sentinel Surveillance - veterinary</subject><subject>Toxoplasma - immunology</subject><subject>Toxoplasma - isolation & purification</subject><subject>Toxoplasma gondii</subject><subject>Toxoplasmosis, Animal - blood</subject><subject>Toxoplasmosis, Animal - epidemiology</subject><subject>Toxoplasmosis, Animal - pathology</subject><subject>Vulpes vulpes</subject><issn>0090-3558</issn><issn>1943-3700</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1Pg0AQhjdGY-vHybvZY42isyx04bihSyVBtqHUtCfCwpLUtFLBHvwB_m-3oh49vTOZJ-8kD0JXBO6Z6_kPNhBiAbNscI7QkPgOtSgDOEZDAB8s6rreAJ113QuA7ZrlFA1sypg3BjpEn3ORylhOo-AOP8lYBIuYp3eYJxM849ljf8LzRfosVliGOJNLOYv5_InjqUwmUYSjJBRBFsnETDhMhbBSnkyjZIpTMcGhXIo5Hj0v4pnJPm4OZCCSLOUxjjIery7QSV1sOn35k-doEYoseLS-3_PYUtSm7xapS-oVriK00ITZbuVWCkjpeE7hedVYqTFlBat8rUF7BvHrWmkfFDB_7EDp03M06nt3bfO21917vl13pd5silfd7LucGHHESGLUoLc9WrZN17W6znftelu0HzmB_OA9P3jPgZl0DH39U7xXW139sb-iDXDTA2rdNK_637IvU1B-hg</recordid><startdate>201307</startdate><enddate>201307</enddate><creator>Verin, Ranieri</creator><creator>Mugnaini, Linda</creator><creator>Nardoni, Simona</creator><creator>Papini, Roberto Amerigo</creator><creator>Ariti, Gaetano</creator><creator>Poli, Alessandro</creator><creator>Mancianti, Francesca</creator><general>Wildlife Disease Association</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>201307</creationdate><title>SEROLOGIC, MOLECULAR, AND PATHOLOGIC SURVEY OF TOXOPLASMA GONDII INFECTION IN FREE-RANGING RED FOXES (VULPES VULPES) IN CENTRAL ITALY</title><author>Verin, Ranieri ; Mugnaini, Linda ; Nardoni, Simona ; Papini, Roberto Amerigo ; Ariti, Gaetano ; Poli, Alessandro ; Mancianti, Francesca</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-b323t-1fc38a5b13ae1725d5db01c484a88d6bb637a7d9ee0e8ae19ffbe90b079640c93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Animals</topic><topic>Animals, Wild - parasitology</topic><topic>Antibodies, Protozoan - blood</topic><topic>Disease Reservoirs - parasitology</topic><topic>Disease Reservoirs - veterinary</topic><topic>Female</topic><topic>Foxes - parasitology</topic><topic>Genotyping</topic><topic>histopathology</topic><topic>IFAT</topic><topic>immunohistochemistry</topic><topic>Immunohistochemistry - veterinary</topic><topic>Italy</topic><topic>Male</topic><topic>PATHOLOGY</topic><topic>PCR</topic><topic>red fox</topic><topic>Sentinel Surveillance - veterinary</topic><topic>Toxoplasma - immunology</topic><topic>Toxoplasma - isolation & purification</topic><topic>Toxoplasma gondii</topic><topic>Toxoplasmosis, Animal - blood</topic><topic>Toxoplasmosis, Animal - epidemiology</topic><topic>Toxoplasmosis, Animal - pathology</topic><topic>Vulpes vulpes</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Verin, Ranieri</creatorcontrib><creatorcontrib>Mugnaini, Linda</creatorcontrib><creatorcontrib>Nardoni, Simona</creatorcontrib><creatorcontrib>Papini, Roberto Amerigo</creatorcontrib><creatorcontrib>Ariti, Gaetano</creatorcontrib><creatorcontrib>Poli, Alessandro</creatorcontrib><creatorcontrib>Mancianti, Francesca</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of wildlife diseases</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Verin, Ranieri</au><au>Mugnaini, Linda</au><au>Nardoni, Simona</au><au>Papini, Roberto Amerigo</au><au>Ariti, Gaetano</au><au>Poli, Alessandro</au><au>Mancianti, Francesca</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>SEROLOGIC, MOLECULAR, AND PATHOLOGIC SURVEY OF TOXOPLASMA GONDII INFECTION IN FREE-RANGING RED FOXES (VULPES VULPES) IN CENTRAL ITALY</atitle><jtitle>Journal of wildlife diseases</jtitle><addtitle>J Wildl Dis</addtitle><date>2013-07</date><risdate>2013</risdate><volume>49</volume><issue>3</issue><spage>545</spage><epage>551</epage><pages>545-551</pages><issn>0090-3558</issn><eissn>1943-3700</eissn><abstract>We tested 191 sera of red foxes (Vulpes vulpes; 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Tissue samples of myocardium, lymph nodes, and brains from antibody-positive animals were tested for T. gondii DNA using specific PCR and processed for histopathology and immunohistochemistry (IHC) to detect T. gondii antigen. Of 192 (53.4%) antibody-positive animals, eight were positive by PCR on myocardium and one on brain. All DNA extracts were genotyped. Histopathology showed lesions characteristic of protozoan encephalitis; IHC did not show T. gondii antigen in examined tissues. The high antibody prevalence found in our study, which is the first in Italy, and the occurrence of polymorphic strains (combination of different type I and III alleles) divergent from typical T. gondii strains, suggests red foxes may be a sentinel of T. gondii in the environment.</abstract><cop>United States</cop><pub>Wildlife Disease Association</pub><pmid>23778603</pmid><doi>10.7589/2011-07-204</doi><tpages>7</tpages></addata></record> |
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| ispartof | Journal of wildlife diseases, 2013-07, Vol.49 (3), p.545-551 |
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| language | eng |
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| source | MEDLINE; Allen Press Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | Animals Animals, Wild - parasitology Antibodies, Protozoan - blood Disease Reservoirs - parasitology Disease Reservoirs - veterinary Female Foxes - parasitology Genotyping histopathology IFAT immunohistochemistry Immunohistochemistry - veterinary Italy Male PATHOLOGY PCR red fox Sentinel Surveillance - veterinary Toxoplasma - immunology Toxoplasma - isolation & purification Toxoplasma gondii Toxoplasmosis, Animal - blood Toxoplasmosis, Animal - epidemiology Toxoplasmosis, Animal - pathology Vulpes vulpes |
| title | SEROLOGIC, MOLECULAR, AND PATHOLOGIC SURVEY OF TOXOPLASMA GONDII INFECTION IN FREE-RANGING RED FOXES (VULPES VULPES) IN CENTRAL ITALY |
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