Evaluation of the fluorescent probes Nile Red and 25‐NBD‐cholesterol as substrates for steroid‐converting oxidoreductases using pure enzymes and microorganisms

The fluorescent probes Nile Red (nonsteroidal dye) and 25‐{N‐[(7‐nitrobenz‐2‐oxa‐1,3‐diazol‐4‐yl)‐methyl]amino}‐27‐norcholesterol (25‐NBD‐cholesterol) (a cholesterol analog) were evaluated as novel substrates for steroid‐converting oxidoreductases. Docking simulations with autodock showed that Nile...

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Veröffentlicht in:	The FEBS journal 2013-07, Vol.280 (13), p.3109-3119
Hauptverfasser:	Faletrov, Yaroslav V., Frolova, Nina S., Hlushko, Hanna V., Rudaya, Elena V., Edimecheva, Irina P., Mauersberger, Stephan, Shkumatov, Vladimir M.
Format:	Artikel
Sprache:	eng
Schlagworte:	25‐{N‐[(7‐nitrobenz‐2‐oxa‐1,3‐diazol‐4‐yl)‐methyl]amino}‐27‐norcholesterol (25‐NBD‐cholesterol) 4-Chloro-7-nitrobenzofurazan - analogs & derivatives 4-Chloro-7-nitrobenzofurazan - chemistry 4-Chloro-7-nitrobenzofurazan - metabolism Alkylation Bacterial Proteins - chemistry Bacterial Proteins - metabolism Brevibacterium - enzymology Brevibacterium - metabolism Catalytic Domain Cholesterol - analogs & derivatives Cholesterol - chemistry Cholesterol - metabolism cholesterol oxidase Cholesterol Oxidase - chemistry Cholesterol Oxidase - metabolism cytochrome P450 17α‐hydroxylase/17,20‐lyase (CYP17A1) Enzymes Fluorescence Fluorescent Dyes - chemistry Fluorescent Dyes - metabolism Fungal Proteins - metabolism Humans Kinetics Microorganisms Molecular Conformation Molecular Docking Simulation NADPH-Ferrihemoprotein Reductase - metabolism Nile Red Oxazines - chemistry Oxazines - metabolism Oxidation-Reduction Oxidoreductases - chemistry Oxidoreductases - metabolism Pseudomonas aeruginosa - enzymology Pseudomonas aeruginosa - metabolism Recombinant Proteins - chemistry Recombinant Proteins - metabolism recombinant yeasts Steroid 17-alpha-Hydroxylase - chemistry Steroid 17-alpha-Hydroxylase - genetics Steroid 17-alpha-Hydroxylase - metabolism Steroids Substrate Specificity Substrates
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