Flow-injection immunosensor for triazine herbicides using Eu(III) chelate label fluorescence detection

An immunochemical flow-injection system for the determination of triazine herbicides based on principles of immunoaffinity chromatography was developed. Triazine herbicide derivatives immobilized on oxirane acrylic beads serve as the affinity column. They are saturated with fluorescently labelled mo...

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Veröffentlicht in:Analytica chimica acta 1994-04, Vol.289 (2), p.177-186
Hauptverfasser: Wortberg, Monika, Middendorf, Cornelia, Katerkamp, Andreas, Rump, Thomas, Krause, Jörg, Cammann, Karl
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container_end_page 186
container_issue 2
container_start_page 177
container_title Analytica chimica acta
container_volume 289
creator Wortberg, Monika
Middendorf, Cornelia
Katerkamp, Andreas
Rump, Thomas
Krause, Jörg
Cammann, Karl
description An immunochemical flow-injection system for the determination of triazine herbicides based on principles of immunoaffinity chromatography was developed. Triazine herbicide derivatives immobilized on oxirane acrylic beads serve as the affinity column. They are saturated with fluorescently labelled monoclonal anti-herbicide antibodies prior to the assay. The label used is the fluorescent Eu(III) chelate W8044-Eu. A fraction of the fluorescent antibodies is replaced when exposed to analyte and this fluorescence is detected in a postcolumn mode by means of a special laser-based fluorimeter. With the reusable affinity column a detection limit of μg l −1 for the herbicide atrazine was obtained.
doi_str_mv 10.1016/0003-2670(94)80101-0
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1873-4324
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source Elsevier ScienceDirect Journals
subjects Analytical chemistry
Chemistry
Chromatographic methods and physical methods associated with chromatography
Chromatography
Exact sciences and technology
Flow injection
Fluorimetry
Herbicides
Immunosensors
Other chromatographic methods
Sensors
Triazine herbicides
title Flow-injection immunosensor for triazine herbicides using Eu(III) chelate label fluorescence detection
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