Flow-injection immunosensor for triazine herbicides using Eu(III) chelate label fluorescence detection
An immunochemical flow-injection system for the determination of triazine herbicides based on principles of immunoaffinity chromatography was developed. Triazine herbicide derivatives immobilized on oxirane acrylic beads serve as the affinity column. They are saturated with fluorescently labelled mo...
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Veröffentlicht in: | Analytica chimica acta 1994-04, Vol.289 (2), p.177-186 |
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creator | Wortberg, Monika Middendorf, Cornelia Katerkamp, Andreas Rump, Thomas Krause, Jörg Cammann, Karl |
description | An immunochemical flow-injection system for the determination of triazine herbicides based on principles of immunoaffinity chromatography was developed. Triazine herbicide derivatives immobilized on oxirane acrylic beads serve as the affinity column. They are saturated with fluorescently labelled monoclonal anti-herbicide antibodies prior to the assay. The label used is the fluorescent Eu(III) chelate W8044-Eu. A fraction of the fluorescent antibodies is replaced when exposed to analyte and this fluorescence is detected in a postcolumn mode by means of a special laser-based fluorimeter. With the reusable affinity column a detection limit of μg l
−1 for the herbicide atrazine was obtained. |
doi_str_mv | 10.1016/0003-2670(94)80101-0 |
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−1 for the herbicide atrazine was obtained.</description><subject>Analytical chemistry</subject><subject>Chemistry</subject><subject>Chromatographic methods and physical methods associated with chromatography</subject><subject>Chromatography</subject><subject>Exact sciences and technology</subject><subject>Flow injection</subject><subject>Fluorimetry</subject><subject>Herbicides</subject><subject>Immunosensors</subject><subject>Other chromatographic methods</subject><subject>Sensors</subject><subject>Triazine herbicides</subject><issn>0003-2670</issn><issn>1873-4324</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><recordid>eNp9kE1LxDAQhoMouH78Aw85iKyH6qRNm_YiyLLqguBFzyGbTjWSTTRpFf31pnbxaCCEGZ55kzyEnDC4YMCqSwAosrwSMG_4eQ2pl8EOmbFaFBkvcr5LZn_IPjmI8TWVOQM-I92N9Z-Zca-oe-MdNZvN4HxEF32gXdp9MOrbOKQvGNZGmxYjHaJxz3Q5zFer1TnVL2hVj9SqNVra2cEHjBqdRtpiP-Uekb1O2YjH2_OQPN0sHxd32f3D7WpxfZ9pXkCftWnlNdeq6YB3SgjBsVECmCjbJi-B5bpmTQnrVOSatQxSs1Ks1awshSqKQ3I25b4F_z5g7OXGpLdYqxz6IUpWVHXSUieQT6AOPsaAnXwLZqPCl2QgR6lyNCZHY7Lh8leqhDR2us1XUSvbBeW0iX-zHAoBdZmwqwnD9NcPg0FGbUYjrQlJiGy9-f-eH11mirQ</recordid><startdate>19940429</startdate><enddate>19940429</enddate><creator>Wortberg, Monika</creator><creator>Middendorf, Cornelia</creator><creator>Katerkamp, Andreas</creator><creator>Rump, Thomas</creator><creator>Krause, Jörg</creator><creator>Cammann, Karl</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QH</scope></search><sort><creationdate>19940429</creationdate><title>Flow-injection immunosensor for triazine herbicides using Eu(III) chelate label fluorescence detection</title><author>Wortberg, Monika ; Middendorf, Cornelia ; Katerkamp, Andreas ; Rump, Thomas ; Krause, Jörg ; Cammann, Karl</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c430t-dddd284ca9f04fa7774e9a70175d925012c81950b9252c1d102506a1dc1557a33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>Analytical chemistry</topic><topic>Chemistry</topic><topic>Chromatographic methods and physical methods associated with chromatography</topic><topic>Chromatography</topic><topic>Exact sciences and technology</topic><topic>Flow injection</topic><topic>Fluorimetry</topic><topic>Herbicides</topic><topic>Immunosensors</topic><topic>Other chromatographic methods</topic><topic>Sensors</topic><topic>Triazine herbicides</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wortberg, Monika</creatorcontrib><creatorcontrib>Middendorf, Cornelia</creatorcontrib><creatorcontrib>Katerkamp, Andreas</creatorcontrib><creatorcontrib>Rump, Thomas</creatorcontrib><creatorcontrib>Krause, Jörg</creatorcontrib><creatorcontrib>Cammann, Karl</creatorcontrib><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>Aqualine</collection><jtitle>Analytica chimica acta</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wortberg, Monika</au><au>Middendorf, Cornelia</au><au>Katerkamp, Andreas</au><au>Rump, Thomas</au><au>Krause, Jörg</au><au>Cammann, Karl</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Flow-injection immunosensor for triazine herbicides using Eu(III) chelate label fluorescence detection</atitle><jtitle>Analytica chimica acta</jtitle><date>1994-04-29</date><risdate>1994</risdate><volume>289</volume><issue>2</issue><spage>177</spage><epage>186</epage><pages>177-186</pages><issn>0003-2670</issn><eissn>1873-4324</eissn><coden>ACACAM</coden><abstract>An immunochemical flow-injection system for the determination of triazine herbicides based on principles of immunoaffinity chromatography was developed. Triazine herbicide derivatives immobilized on oxirane acrylic beads serve as the affinity column. They are saturated with fluorescently labelled monoclonal anti-herbicide antibodies prior to the assay. The label used is the fluorescent Eu(III) chelate W8044-Eu. A fraction of the fluorescent antibodies is replaced when exposed to analyte and this fluorescence is detected in a postcolumn mode by means of a special laser-based fluorimeter. With the reusable affinity column a detection limit of μg l
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subjects | Analytical chemistry Chemistry Chromatographic methods and physical methods associated with chromatography Chromatography Exact sciences and technology Flow injection Fluorimetry Herbicides Immunosensors Other chromatographic methods Sensors Triazine herbicides |
title | Flow-injection immunosensor for triazine herbicides using Eu(III) chelate label fluorescence detection |
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