Nonlinear Microscopy of Eumelanin and Pheomelanin with Subcellular Resolution
Pump–probe microscopy nondestructively differentiates eumelanin and pheomelanin and can be used to quantify melanin distributions in thin biopsy slices. Here we have extended that work for imaging eumelanin and pheomelanin distributions on a subcellular scale, allowing elucidation of characteristics...
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| Veröffentlicht in: | Journal of investigative dermatology 2013-07, Vol.133 (7), p.1822-1826 |
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| container_issue | 7 |
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| container_title | Journal of investigative dermatology |
| container_volume | 133 |
| creator | Simpson, Mary Jane Wilson, Jesse W. Anthony Phipps, M. Robles, Francisco E. Angelica Selim, M. Warren, Warren S. |
| description | Pump–probe microscopy nondestructively differentiates eumelanin and pheomelanin and can be used to quantify melanin distributions in thin biopsy slices. Here we have extended that work for imaging eumelanin and pheomelanin distributions on a subcellular scale, allowing elucidation of characteristics of different cell types. The results show that melanin heterogeneity, previously found to be characteristic of melanomas, persists on the subcellular scale. We have also found spectral changes associated with melanin located in melanophages that could potentially differentiate invasive pigmented melanocytes from melanophages without immunohistochemical staining. |
| doi_str_mv | 10.1038/jid.2013.37 |
| format | Article |
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Here we have extended that work for imaging eumelanin and pheomelanin distributions on a subcellular scale, allowing elucidation of characteristics of different cell types. The results show that melanin heterogeneity, previously found to be characteristic of melanomas, persists on the subcellular scale. 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We have also found spectral changes associated with melanin located in melanophages that could potentially differentiate invasive pigmented melanocytes from melanophages without immunohistochemical staining.</description><subject>Biopsy</subject><subject>Humans</subject><subject>Keratinocytes - metabolism</subject><subject>Keratinocytes - pathology</subject><subject>Macrophages - metabolism</subject><subject>Macrophages - pathology</subject><subject>Melanins - metabolism</subject><subject>Melanocytes - metabolism</subject><subject>Melanocytes - pathology</subject><subject>Melanoma - metabolism</subject><subject>Melanoma - pathology</subject><subject>Microscopy, Fluorescence - methods</subject><subject>Skin Neoplasms - metabolism</subject><subject>Skin Neoplasms - pathology</subject><subject>Spectroscopy, Near-Infrared - methods</subject><issn>0022-202X</issn><issn>1523-1747</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNptkEtLxDAUhYMoOo6u3EvBjSAd8266FPEFvvAB7kKa3mKGTjImreK_t2XUhbi6XPjO4fAhtEfwjGCmjueunlFM2IwVa2hCBGU5KXixjiYYU5pTTF-20HZKc4yJ5EJtoi3KmGClEhN0cxt86zyYmN04G0OyYfmZhSY76xfQGu98Znyd3b9C-Pk_XPeaPfaVhbbt2yH4ACm0feeC30EbjWkT7H7fKXo-P3s6vcyv7y6uTk-uc8sx7nICFQfLSSUqToyQRja8FKypuJTcNMwwJquSlpzWlQJFSmgMK2srwDRECsmm6HDVu4zhrYfU6YVL4x7jIfRJEyYLpbAcrEzRwR90Hvroh3UjJZUSjJYDdbSiRgUpQqOX0S1M_NQE69GyHizr0bJmxUDvf3f21QLqX_ZH6wCIFQCDhHcHUSfrwFuoXQTb6Tq4f4u_AAgVigI</recordid><startdate>20130701</startdate><enddate>20130701</enddate><creator>Simpson, Mary Jane</creator><creator>Wilson, Jesse W.</creator><creator>Anthony Phipps, M.</creator><creator>Robles, Francisco E.</creator><creator>Angelica Selim, M.</creator><creator>Warren, Warren S.</creator><general>Elsevier Inc</general><general>Elsevier Limited</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7T5</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>H94</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>P64</scope><scope>PHGZM</scope><scope>PHGZT</scope><scope>PJZUB</scope><scope>PKEHL</scope><scope>PPXIY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20130701</creationdate><title>Nonlinear Microscopy of Eumelanin and Pheomelanin with Subcellular Resolution</title><author>Simpson, Mary Jane ; 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| subjects | Biopsy Humans Keratinocytes - metabolism Keratinocytes - pathology Macrophages - metabolism Macrophages - pathology Melanins - metabolism Melanocytes - metabolism Melanocytes - pathology Melanoma - metabolism Melanoma - pathology Microscopy, Fluorescence - methods Skin Neoplasms - metabolism Skin Neoplasms - pathology Spectroscopy, Near-Infrared - methods |
| title | Nonlinear Microscopy of Eumelanin and Pheomelanin with Subcellular Resolution |
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