Synthesis of a New Conjugated Polymer for DNA Alkylation and Gene Regulation
A new polyfluorene derivative containing pendent alkylating chlorambucil (PFP-Cbl) was synthesized and characterized. Under direct incubation with DNA in vitro, PFP-Cbl could undergo an efficient DNA alkylating reaction and induce DNA cross-linking. In vitro transcription and translation experiment...
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| Veröffentlicht in: | ACS applied materials & interfaces 2013-06, Vol.5 (11), p.4549-4554 |
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| container_title | ACS applied materials & interfaces |
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| creator | Nie, Chenyao Zhu, Chunlei Feng, Liheng Lv, Fengting Liu, Libing Wang, Shu |
| description | A new polyfluorene derivative containing pendent alkylating chlorambucil (PFP-Cbl) was synthesized and characterized. Under direct incubation with DNA in vitro, PFP-Cbl could undergo an efficient DNA alkylating reaction and induce DNA cross-linking. In vitro transcription and translation experiment exhibited that the PFP-Cbl significantly down-regulated the gene expression of luciferase reporter plasmid. The down-regulation of gene expression was also verified through the transfection experiment of p-EGFP plasmid, which showed decreased green fluorescent protein (GFP) in cells. Meanwhile, the self-luminous property of PFP-Cbl could make it able to trace the internalized PFP-Cbl and plasmid complexes resulted from cross-linking in cells by fluorescent microscopy. Combining the features of alkylating function, multivalent binding sites, and fluorescent characteristics, PFP-Cbl provides a new insight in the area of gene regulation and extends the new applications of conjugated polymers (CPs). |
| doi_str_mv | 10.1021/am4001985 |
| format | Article |
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Under direct incubation with DNA in vitro, PFP-Cbl could undergo an efficient DNA alkylating reaction and induce DNA cross-linking. In vitro transcription and translation experiment exhibited that the PFP-Cbl significantly down-regulated the gene expression of luciferase reporter plasmid. The down-regulation of gene expression was also verified through the transfection experiment of p-EGFP plasmid, which showed decreased green fluorescent protein (GFP) in cells. Meanwhile, the self-luminous property of PFP-Cbl could make it able to trace the internalized PFP-Cbl and plasmid complexes resulted from cross-linking in cells by fluorescent microscopy. 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Mater. Interfaces</addtitle><description>A new polyfluorene derivative containing pendent alkylating chlorambucil (PFP-Cbl) was synthesized and characterized. Under direct incubation with DNA in vitro, PFP-Cbl could undergo an efficient DNA alkylating reaction and induce DNA cross-linking. In vitro transcription and translation experiment exhibited that the PFP-Cbl significantly down-regulated the gene expression of luciferase reporter plasmid. The down-regulation of gene expression was also verified through the transfection experiment of p-EGFP plasmid, which showed decreased green fluorescent protein (GFP) in cells. Meanwhile, the self-luminous property of PFP-Cbl could make it able to trace the internalized PFP-Cbl and plasmid complexes resulted from cross-linking in cells by fluorescent microscopy. Combining the features of alkylating function, multivalent binding sites, and fluorescent characteristics, PFP-Cbl provides a new insight in the area of gene regulation and extends the new applications of conjugated polymers (CPs).</description><subject>Alkylation</subject><subject>Antineoplastic Agents, Alkylating - chemical synthesis</subject><subject>Binding Sites - genetics</subject><subject>Chlorambucil - chemical synthesis</subject><subject>Cross-Linking Reagents - chemical synthesis</subject><subject>DNA - genetics</subject><subject>DNA - metabolism</subject><subject>Gene Expression Regulation</subject><subject>Green Fluorescent Proteins - genetics</subject><subject>Green Fluorescent Proteins - metabolism</subject><subject>HeLa Cells</subject><subject>Humans</subject><subject>Microscopy, Fluorescence</subject><subject>Plasmids - genetics</subject><subject>Plasmids - metabolism</subject><subject>Polymers - chemical synthesis</subject><subject>Polymers - metabolism</subject><issn>1944-8244</issn><issn>1944-8252</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNptkE1Lw0AQQBdRrFYP_gHZi6CH6O5mN9kcS9UqlCp-nMMkO6mtSbbuJkj-vZHUnjzNMDwezCPkjLNrzgS_gUoyxhOt9sgRT6QMtFBif7dLOSLH3q8Zi0LB1CEZiVBJzZk8IvPXrm4-0K88tQUFusBvOrX1ul1Cg4Y-27Kr0NHCOnq7mNBJ-dmV0KxsTaE2dIY10hdctsPthBwUUHo83c4xeb-_e5s-BPOn2eN0Mg8g5KoJEoWxDjVwlLqAJEkyqZQ0yvA8KjImMtSRMTKLi5BrJmJQOUJktIJEACRxOCaXg3fj7FeLvkmrlc-xLKFG2_qUh1GsmJKc9ejVgObOeu-wSDduVYHrUs7S33jpLl7Pnm-1bVah2ZF_tXrgYgAg9-natq7uv_xH9AMOlHPs</recordid><startdate>20130612</startdate><enddate>20130612</enddate><creator>Nie, Chenyao</creator><creator>Zhu, Chunlei</creator><creator>Feng, Liheng</creator><creator>Lv, Fengting</creator><creator>Liu, Libing</creator><creator>Wang, Shu</creator><general>American Chemical Society</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20130612</creationdate><title>Synthesis of a New Conjugated Polymer for DNA Alkylation and Gene Regulation</title><author>Nie, Chenyao ; Zhu, Chunlei ; Feng, Liheng ; Lv, Fengting ; Liu, Libing ; Wang, Shu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a315t-95e7838a1e48fa999b4554d5d1c6fb02be86dd4b7f318027a5cea6d85a92aa973</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Alkylation</topic><topic>Antineoplastic Agents, Alkylating - chemical synthesis</topic><topic>Binding Sites - genetics</topic><topic>Chlorambucil - chemical synthesis</topic><topic>Cross-Linking Reagents - chemical synthesis</topic><topic>DNA - genetics</topic><topic>DNA - metabolism</topic><topic>Gene Expression Regulation</topic><topic>Green Fluorescent Proteins - genetics</topic><topic>Green Fluorescent Proteins - metabolism</topic><topic>HeLa Cells</topic><topic>Humans</topic><topic>Microscopy, Fluorescence</topic><topic>Plasmids - genetics</topic><topic>Plasmids - metabolism</topic><topic>Polymers - chemical synthesis</topic><topic>Polymers - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Nie, Chenyao</creatorcontrib><creatorcontrib>Zhu, Chunlei</creatorcontrib><creatorcontrib>Feng, Liheng</creatorcontrib><creatorcontrib>Lv, Fengting</creatorcontrib><creatorcontrib>Liu, Libing</creatorcontrib><creatorcontrib>Wang, Shu</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>ACS applied materials & interfaces</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Nie, Chenyao</au><au>Zhu, Chunlei</au><au>Feng, Liheng</au><au>Lv, Fengting</au><au>Liu, Libing</au><au>Wang, Shu</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Synthesis of a New Conjugated Polymer for DNA Alkylation and Gene Regulation</atitle><jtitle>ACS applied materials & interfaces</jtitle><addtitle>ACS Appl. Mater. Interfaces</addtitle><date>2013-06-12</date><risdate>2013</risdate><volume>5</volume><issue>11</issue><spage>4549</spage><epage>4554</epage><pages>4549-4554</pages><issn>1944-8244</issn><eissn>1944-8252</eissn><abstract>A new polyfluorene derivative containing pendent alkylating chlorambucil (PFP-Cbl) was synthesized and characterized. Under direct incubation with DNA in vitro, PFP-Cbl could undergo an efficient DNA alkylating reaction and induce DNA cross-linking. In vitro transcription and translation experiment exhibited that the PFP-Cbl significantly down-regulated the gene expression of luciferase reporter plasmid. The down-regulation of gene expression was also verified through the transfection experiment of p-EGFP plasmid, which showed decreased green fluorescent protein (GFP) in cells. Meanwhile, the self-luminous property of PFP-Cbl could make it able to trace the internalized PFP-Cbl and plasmid complexes resulted from cross-linking in cells by fluorescent microscopy. Combining the features of alkylating function, multivalent binding sites, and fluorescent characteristics, PFP-Cbl provides a new insight in the area of gene regulation and extends the new applications of conjugated polymers (CPs).</abstract><cop>United States</cop><pub>American Chemical Society</pub><pmid>23548104</pmid><doi>10.1021/am4001985</doi><tpages>6</tpages></addata></record> |
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| subjects | Alkylation Antineoplastic Agents, Alkylating - chemical synthesis Binding Sites - genetics Chlorambucil - chemical synthesis Cross-Linking Reagents - chemical synthesis DNA - genetics DNA - metabolism Gene Expression Regulation Green Fluorescent Proteins - genetics Green Fluorescent Proteins - metabolism HeLa Cells Humans Microscopy, Fluorescence Plasmids - genetics Plasmids - metabolism Polymers - chemical synthesis Polymers - metabolism |
| title | Synthesis of a New Conjugated Polymer for DNA Alkylation and Gene Regulation |
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