Sensitive determination of enzymatically labile dissolved organic phosphorus and its vertical profiles in the oligotrophic western North Pacific and East China Sea

Trace concentrations of labile dissolved organic phosphorus (LDOP) in oligotrophic seawater were measured by use of an enzymatic procedure and a nanomolar phosphate analytical system consisting of a gas-segmented continuous flow analyzer with a liquid waveguide capillary cell. LDOP, defined as DOP h...

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Veröffentlicht in:Journal of oceanography 2013-06, Vol.69 (3), p.357-367
Hauptverfasser: Hashihama, Fuminori, Kinouchi, Shinko, Suwa, Shuhei, Suzumura, Masahiro, Kanda, Jota
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container_end_page 367
container_issue 3
container_start_page 357
container_title Journal of oceanography
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creator Hashihama, Fuminori
Kinouchi, Shinko
Suwa, Shuhei
Suzumura, Masahiro
Kanda, Jota
description Trace concentrations of labile dissolved organic phosphorus (LDOP) in oligotrophic seawater were measured by use of an enzymatic procedure and a nanomolar phosphate analytical system consisting of a gas-segmented continuous flow analyzer with a liquid waveguide capillary cell. LDOP, defined as DOP hydrolyzed by alkaline phosphatase (AP) from Escherichia coli , was quantified as the difference between the phosphate concentrations of the seawater sample with and without AP treatment. For sensitive measurement of LDOP, we found that phosphate contamination derived from commercially available AP must be corrected, and azide treatment before AP treatment proved effective in removing biological effect that occurs during DOP hydrolysis. Field observations at six stations of the western North Pacific and the East China Sea during the boreal summer revealed that, in the upper 200 m of the water column, LDOP concentrations ranged from the detection limit (3 n M ) to 243 n M , and phosphate concentrations ranged from 5 to 374 n M . The spatial distribution patterns of LDOP were similar to those of phosphate. Most of the depth profiles for LDOP and phosphate showed concentrations were extremely low,
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Most of the depth profiles for LDOP and phosphate showed concentrations were extremely low, &lt;25 n M , between the surface and the deep chlorophyll maximum layer (DCML) and increased below the DCML. 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subjects Biological effects
Chemical analysis
Chemical oceanography
Continuous flow
Dissolved organic phosphorus
Distribution patterns
E coli
Earth and Environmental Science
Earth Sciences
Escherichia coli
Freshwater & Marine Ecology
Marine
Oceanography
Original Article
Phosphates
Phosphorus content
Seawater
Spatial distribution
Water analysis
Water column
title Sensitive determination of enzymatically labile dissolved organic phosphorus and its vertical profiles in the oligotrophic western North Pacific and East China Sea
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