Distribution of additional virulence factors related to adhesion and toxicity in Shiga toxin‐producing Escherichia coli isolated from raw products in Argentina
A total of 73 Shiga toxin‐producing Escherichia coli (STEC) isolates, belonging to 25 serotypes and isolated from raw products in Argentina, were examined for the occurrence of genes responsible for bacterial adhesions to intestine, ehaA (EHEC autotransporter), lpfAO113 (long polar fimbriae), sab (S...
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| Veröffentlicht in: | Letters in applied microbiology 2013-06, Vol.56 (6), p.449-455 |
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| creator | Cadona, J.S. Bustamante, A.V. Parma, A.E. Lucchesi, P.M.A. Sanso, A.M. |
| description | A total of 73 Shiga toxin‐producing Escherichia coli (STEC) isolates, belonging to 25 serotypes and isolated from raw products in Argentina, were examined for the occurrence of genes responsible for bacterial adhesions to intestine, ehaA (EHEC autotransporter), lpfAO113 (long polar fimbriae), sab (STEC autotransporter [AT] contributing to biofilm formation), ecpA (E. coli common pilus), hcpA (haemorrhagic coli pilus), elfA (E. coli laminin‐binding fimbriae), sfpA (sorbitol‐fermenting EHEC O157 fimbriae plasmid‐encoded) and of the toxigenic gene cdt‐V (cytolethal distending toxin). Our study showed different adhesin profiles that are not linked to one specific serotype and that all analysed isolates possess, besides stx genes, some adherence genes. Several of the isolates contained also multiple toxin genes. The results of the present work alert the presence of genes coding for additional adhesins and cdt‐V toxin in LEE‐negative STEC strains that occur in foods, and this traits could increase their pathogenic potential.
Significance and Impact of the Study
Meat products are one of the main vehicles of Shiga toxin‐producing E. coli, and the presence of genes coding for additional adhesins and toxins could increase their pathogenic potential. There is a need for a more detailed characterization of the strains in regard to these extra virulence factors.
Significance and Impact of the Study: Meat products are one of the main vehicles of Shiga toxin‐producing E. coli, and the presence of genes coding for additional adhesins and toxins could increase their pathogenic potential. There is a need for a more detailed characterization of the strains in regard to these extra virulence factors. |
| doi_str_mv | 10.1111/lam.12067 |
| format | Article |
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Significance and Impact of the Study
Meat products are one of the main vehicles of Shiga toxin‐producing E. coli, and the presence of genes coding for additional adhesins and toxins could increase their pathogenic potential. There is a need for a more detailed characterization of the strains in regard to these extra virulence factors.
Significance and Impact of the Study: Meat products are one of the main vehicles of Shiga toxin‐producing E. coli, and the presence of genes coding for additional adhesins and toxins could increase their pathogenic potential. There is a need for a more detailed characterization of the strains in regard to these extra virulence factors.</description><identifier>ISSN: 0266-8254</identifier><identifier>EISSN: 1472-765X</identifier><identifier>DOI: 10.1111/lam.12067</identifier><identifier>PMID: 23488818</identifier><identifier>CODEN: LAMIE7</identifier><language>eng</language><publisher>Oxford: Blackwell</publisher><subject>Adhesins, Bacterial - genetics ; adhesion ; Argentina ; bacterial toxin ; Bacterial Toxins - genetics ; Biological and medical sciences ; Escherichia coli ; Escherichia coli - genetics ; Escherichia coli - isolation & purification ; Escherichia coli - pathogenicity ; Escherichia coli Proteins - genetics ; food ; Fundamental and applied biological sciences. Psychology ; Genes, Bacterial ; Meat Products - microbiology ; Microbiology ; Shiga Toxins - genetics ; Shiga toxin‐producing Escherichia coli ; Shiga-Toxigenic Escherichia coli - classification ; Shiga-Toxigenic Escherichia coli - genetics ; Shiga-Toxigenic Escherichia coli - isolation & purification ; Shiga-Toxigenic Escherichia coli - pathogenicity ; Virulence Factors - genetics ; virulence genes</subject><ispartof>Letters in applied microbiology, 2013-06, Vol.56 (6), p.449-455</ispartof><rights>2013 The Society for Applied Microbiology</rights><rights>2014 INIST-CNRS</rights><rights>2013 The Society for Applied Microbiology.</rights><rights>Copyright © 2013 The Society for Applied Microbiology</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4517-95e61af5e78ff6dd657a0857c67780668cedb9a49ca18a0bfd456df531e8f8963</citedby><cites>FETCH-LOGICAL-c4517-95e61af5e78ff6dd657a0857c67780668cedb9a49ca18a0bfd456df531e8f8963</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Flam.12067$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Flam.12067$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=27369197$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23488818$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Cadona, J.S.</creatorcontrib><creatorcontrib>Bustamante, A.V.</creatorcontrib><creatorcontrib>Parma, A.E.</creatorcontrib><creatorcontrib>Lucchesi, P.M.A.</creatorcontrib><creatorcontrib>Sanso, A.M.</creatorcontrib><title>Distribution of additional virulence factors related to adhesion and toxicity in Shiga toxin‐producing Escherichia coli isolated from raw products in Argentina</title><title>Letters in applied microbiology</title><addtitle>Lett Appl Microbiol</addtitle><description>A total of 73 Shiga toxin‐producing Escherichia coli (STEC) isolates, belonging to 25 serotypes and isolated from raw products in Argentina, were examined for the occurrence of genes responsible for bacterial adhesions to intestine, ehaA (EHEC autotransporter), lpfAO113 (long polar fimbriae), sab (STEC autotransporter [AT] contributing to biofilm formation), ecpA (E. coli common pilus), hcpA (haemorrhagic coli pilus), elfA (E. coli laminin‐binding fimbriae), sfpA (sorbitol‐fermenting EHEC O157 fimbriae plasmid‐encoded) and of the toxigenic gene cdt‐V (cytolethal distending toxin). Our study showed different adhesin profiles that are not linked to one specific serotype and that all analysed isolates possess, besides stx genes, some adherence genes. Several of the isolates contained also multiple toxin genes. The results of the present work alert the presence of genes coding for additional adhesins and cdt‐V toxin in LEE‐negative STEC strains that occur in foods, and this traits could increase their pathogenic potential.
Significance and Impact of the Study
Meat products are one of the main vehicles of Shiga toxin‐producing E. coli, and the presence of genes coding for additional adhesins and toxins could increase their pathogenic potential. There is a need for a more detailed characterization of the strains in regard to these extra virulence factors.
Significance and Impact of the Study: Meat products are one of the main vehicles of Shiga toxin‐producing E. coli, and the presence of genes coding for additional adhesins and toxins could increase their pathogenic potential. There is a need for a more detailed characterization of the strains in regard to these extra virulence factors.</description><subject>Adhesins, Bacterial - genetics</subject><subject>adhesion</subject><subject>Argentina</subject><subject>bacterial toxin</subject><subject>Bacterial Toxins - genetics</subject><subject>Biological and medical sciences</subject><subject>Escherichia coli</subject><subject>Escherichia coli - genetics</subject><subject>Escherichia coli - isolation & purification</subject><subject>Escherichia coli - pathogenicity</subject><subject>Escherichia coli Proteins - genetics</subject><subject>food</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genes, Bacterial</subject><subject>Meat Products - microbiology</subject><subject>Microbiology</subject><subject>Shiga Toxins - genetics</subject><subject>Shiga toxin‐producing Escherichia coli</subject><subject>Shiga-Toxigenic Escherichia coli - classification</subject><subject>Shiga-Toxigenic Escherichia coli - genetics</subject><subject>Shiga-Toxigenic Escherichia coli - isolation & purification</subject><subject>Shiga-Toxigenic Escherichia coli - pathogenicity</subject><subject>Virulence Factors - genetics</subject><subject>virulence genes</subject><issn>0266-8254</issn><issn>1472-765X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp10ctqVDEYB_Agih2rC19AAlKoi9Mm55LLcqj1AiMuVHB3-CaXmZRMUpNzbGfnI_gKvppPYk7PqCCYTS788v9CPoSeUnJGyzj3sDujNWH8HlrQltcVZ93n-2hBasYqUXftEXqU8xUhRNBaPkRHddMKIahYoB8vXR6SW4-DiwFHi0FrN63B468ujd4EZbAFNcSUcTIeBqPxEIvbmjzdgTDtb51ywx67gD9s3QbuTsLPb9-vU9SjcmGDL7PamuTU1gFW0TvscpzTbIo7nOAGz3jIU8wybUwYXIDH6IEFn82Tw3yMPr26_Hjxplq9f_32YrmqVNtRXsnOMAq2M1xYy7RmHQciOq4Y54IwJpTRawmtVEAFkLXVbce07RpqhBWSNcfodM4tr_gymjz0O5eV8R6CiWPuacN4KymRTaHP_6FXcUzlyyZVHsMok6KoF7NSKeacjO2vk9tB2veU9FPf-tK3_q5vxT47JI7rndF_5O9GFXByAJAVeJsgKJf_Ot4wSeUUdD67G-fN_v8V-9Xy3Vz6F6L9swI</recordid><startdate>201306</startdate><enddate>201306</enddate><creator>Cadona, J.S.</creator><creator>Bustamante, A.V.</creator><creator>Parma, A.E.</creator><creator>Lucchesi, P.M.A.</creator><creator>Sanso, A.M.</creator><general>Blackwell</general><general>Oxford University Press</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7ST</scope><scope>7T7</scope><scope>7TM</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>SOI</scope><scope>7U7</scope></search><sort><creationdate>201306</creationdate><title>Distribution of additional virulence factors related to adhesion and toxicity in Shiga toxin‐producing Escherichia coli isolated from raw products in Argentina</title><author>Cadona, J.S. ; Bustamante, A.V. ; Parma, A.E. ; Lucchesi, P.M.A. ; Sanso, A.M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4517-95e61af5e78ff6dd657a0857c67780668cedb9a49ca18a0bfd456df531e8f8963</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Adhesins, Bacterial - genetics</topic><topic>adhesion</topic><topic>Argentina</topic><topic>bacterial toxin</topic><topic>Bacterial Toxins - genetics</topic><topic>Biological and medical sciences</topic><topic>Escherichia coli</topic><topic>Escherichia coli - genetics</topic><topic>Escherichia coli - isolation & purification</topic><topic>Escherichia coli - pathogenicity</topic><topic>Escherichia coli Proteins - genetics</topic><topic>food</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genes, Bacterial</topic><topic>Meat Products - microbiology</topic><topic>Microbiology</topic><topic>Shiga Toxins - genetics</topic><topic>Shiga toxin‐producing Escherichia coli</topic><topic>Shiga-Toxigenic Escherichia coli - classification</topic><topic>Shiga-Toxigenic Escherichia coli - genetics</topic><topic>Shiga-Toxigenic Escherichia coli - isolation & purification</topic><topic>Shiga-Toxigenic Escherichia coli - pathogenicity</topic><topic>Virulence Factors - genetics</topic><topic>virulence genes</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Cadona, J.S.</creatorcontrib><creatorcontrib>Bustamante, A.V.</creatorcontrib><creatorcontrib>Parma, A.E.</creatorcontrib><creatorcontrib>Lucchesi, P.M.A.</creatorcontrib><creatorcontrib>Sanso, A.M.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Environment Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environment Abstracts</collection><collection>Toxicology Abstracts</collection><jtitle>Letters in applied microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Cadona, J.S.</au><au>Bustamante, A.V.</au><au>Parma, A.E.</au><au>Lucchesi, P.M.A.</au><au>Sanso, A.M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Distribution of additional virulence factors related to adhesion and toxicity in Shiga toxin‐producing Escherichia coli isolated from raw products in Argentina</atitle><jtitle>Letters in applied microbiology</jtitle><addtitle>Lett Appl Microbiol</addtitle><date>2013-06</date><risdate>2013</risdate><volume>56</volume><issue>6</issue><spage>449</spage><epage>455</epage><pages>449-455</pages><issn>0266-8254</issn><eissn>1472-765X</eissn><coden>LAMIE7</coden><abstract>A total of 73 Shiga toxin‐producing Escherichia coli (STEC) isolates, belonging to 25 serotypes and isolated from raw products in Argentina, were examined for the occurrence of genes responsible for bacterial adhesions to intestine, ehaA (EHEC autotransporter), lpfAO113 (long polar fimbriae), sab (STEC autotransporter [AT] contributing to biofilm formation), ecpA (E. coli common pilus), hcpA (haemorrhagic coli pilus), elfA (E. coli laminin‐binding fimbriae), sfpA (sorbitol‐fermenting EHEC O157 fimbriae plasmid‐encoded) and of the toxigenic gene cdt‐V (cytolethal distending toxin). Our study showed different adhesin profiles that are not linked to one specific serotype and that all analysed isolates possess, besides stx genes, some adherence genes. Several of the isolates contained also multiple toxin genes. The results of the present work alert the presence of genes coding for additional adhesins and cdt‐V toxin in LEE‐negative STEC strains that occur in foods, and this traits could increase their pathogenic potential.
Significance and Impact of the Study
Meat products are one of the main vehicles of Shiga toxin‐producing E. coli, and the presence of genes coding for additional adhesins and toxins could increase their pathogenic potential. There is a need for a more detailed characterization of the strains in regard to these extra virulence factors.
Significance and Impact of the Study: Meat products are one of the main vehicles of Shiga toxin‐producing E. coli, and the presence of genes coding for additional adhesins and toxins could increase their pathogenic potential. There is a need for a more detailed characterization of the strains in regard to these extra virulence factors.</abstract><cop>Oxford</cop><pub>Blackwell</pub><pmid>23488818</pmid><doi>10.1111/lam.12067</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Access via Wiley Online Library; Oxford University Press Journals All Titles (1996-Current); EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Adhesins, Bacterial - genetics adhesion Argentina bacterial toxin Bacterial Toxins - genetics Biological and medical sciences Escherichia coli Escherichia coli - genetics Escherichia coli - isolation & purification Escherichia coli - pathogenicity Escherichia coli Proteins - genetics food Fundamental and applied biological sciences. Psychology Genes, Bacterial Meat Products - microbiology Microbiology Shiga Toxins - genetics Shiga toxin‐producing Escherichia coli Shiga-Toxigenic Escherichia coli - classification Shiga-Toxigenic Escherichia coli - genetics Shiga-Toxigenic Escherichia coli - isolation & purification Shiga-Toxigenic Escherichia coli - pathogenicity Virulence Factors - genetics virulence genes |
| title | Distribution of additional virulence factors related to adhesion and toxicity in Shiga toxin‐producing Escherichia coli isolated from raw products in Argentina |
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