Porphyromonas gingivalis biofilms persist after chlorhexidine treatment

Chlorhexidine (CHX) gluconate effectively reduces the viability of biofilm‐forming bacteria, such as Porphyromonas gingivalis. However, it is impossible to completely remove biofilms. The goal of the present study was to assess the potential pathogenicity of residual P. gingivalis biofilms in vitro...

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Veröffentlicht in:European journal of oral sciences 2013-06, Vol.121 (3pt1), p.162-168
Hauptverfasser: Yamaguchi, Mikiyo, Noiri, Yuichiro, Kuboniwa, Masae, Yamamoto, Reiko, Asahi, Yoko, Maezono, Hazuki, Hayashi, Mikako, Ebisu, Shigeyuki
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container_end_page 168
container_issue 3pt1
container_start_page 162
container_title European journal of oral sciences
container_volume 121
creator Yamaguchi, Mikiyo
Noiri, Yuichiro
Kuboniwa, Masae
Yamamoto, Reiko
Asahi, Yoko
Maezono, Hazuki
Hayashi, Mikako
Ebisu, Shigeyuki
description Chlorhexidine (CHX) gluconate effectively reduces the viability of biofilm‐forming bacteria, such as Porphyromonas gingivalis. However, it is impossible to completely remove biofilms. The goal of the present study was to assess the potential pathogenicity of residual P. gingivalis biofilms in vitro after treatment with CHX gluconate. Scanning and transmission electron microscopy and confocal laser imaging revealed that treatment with CHX gluconate disrupted individual biofilm‐forming P. gingivalis cells but did not destroy the biofilms. The volumes of the protein and carbohydrate constituents in the residual biofilms were not significantly different from those of the controls. The physical resistance of the residual biofilms to ultrasonication was significantly higher than that of controls. The volume of P. gingivalis adherent to the residual biofilms was higher than that to saliva‐coated wells. These findings suggest that although CHX gluconate caused disruption of biofilm‐forming cells, the constituents derived from disrupted cells were maintained in the biofilms, which sustained their external structures. Moreover, the residual biofilms could serve as a scaffold for the formation of new biofilms.
doi_str_mv 10.1111/eos.12050
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subjects Anti-Infective Agents, Local - pharmacology
antimicrobial agents
Bacterial Adhesion - drug effects
Bacterial Proteins - physiology
biofilms
Biofilms - drug effects
chlorhexidine
Chlorhexidine - pharmacology
Dentistry
High-Energy Shock Waves
Imaging, Three-Dimensional - methods
Microbial Viability - drug effects
Polysaccharides, Bacterial - physiology
Porphyromonas gingivalis
Porphyromonas gingivalis - chemistry
Porphyromonas gingivalis - drug effects
Porphyromonas gingivalis - pathogenicity
title Porphyromonas gingivalis biofilms persist after chlorhexidine treatment
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