Cloning and application of recombinant dengue virus prM-M protein for serodiagnosis of dengue virus infection
We studied the use of the precursor to the M structural protein (prM) found only on the surface of mature dengue virus as a target protein to detect dengue virus infection. Recombinant D2-16681 prM-M protein was constructed and tested for immunogenicity with dengue and Japanese encephalitis patient...
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| Veröffentlicht in: | Southeast Asian journal of tropical medicine and public health 2013-03, Vol.44 (2), p.218-225 |
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| creator | Wichit, Sineewanlaya Yongpradoem, Hatairat Surasombatpattana, Pornapat Leaungwuttiwong, Pornsawan Kalambaheti, Thareerat Jampangern, Wipawee Jittmittraphap, Akanitt |
| description | We studied the use of the precursor to the M structural protein (prM) found only on the surface of mature dengue virus as a target protein to detect dengue virus infection. Recombinant D2-16681 prM-M protein was constructed and tested for immunogenicity with dengue and Japanese encephalitis patient sera by Western blot analysis and indirect ELISA. The sensitivity and specificity of indirect ELISA were 48.1 and 85.5%, respectively, and Western blot assay were 23.1 and 98.7%, respectively, for detection of dengue virus. Although the sensitivity of the indirect ELISA is low, the indirect ELISA using recombinant D2-16681 prM-M proteins as antigen may be used for early detection of dengue virus infection. |
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Recombinant D2-16681 prM-M protein was constructed and tested for immunogenicity with dengue and Japanese encephalitis patient sera by Western blot analysis and indirect ELISA. The sensitivity and specificity of indirect ELISA were 48.1 and 85.5%, respectively, and Western blot assay were 23.1 and 98.7%, respectively, for detection of dengue virus. Although the sensitivity of the indirect ELISA is low, the indirect ELISA using recombinant D2-16681 prM-M proteins as antigen may be used for early detection of dengue virus infection.</description><identifier>ISSN: 0125-1562</identifier><identifier>PMID: 23691631</identifier><language>eng</language><publisher>Thailand: Central Coordinating Board, SEAMEO-TROPMED Project</publisher><subject>Cloning, Molecular ; Dengue - blood ; Dengue - diagnosis ; Dengue - immunology ; Dengue virus ; Dengue Virus - genetics ; Dengue Virus - immunology ; Enzyme-Linked Immunosorbent Assay ; Humans ; Recombinant Proteins - genetics ; Serologic Tests ; Viral Proteins - genetics ; Viral Proteins - immunology</subject><ispartof>Southeast Asian journal of tropical medicine and public health, 2013-03, Vol.44 (2), p.218-225</ispartof><rights>Copyright Central Coordinating Board, SEAMEO-TROPMED Project Mar 2013</rights><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23691631$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wichit, Sineewanlaya</creatorcontrib><creatorcontrib>Yongpradoem, Hatairat</creatorcontrib><creatorcontrib>Surasombatpattana, Pornapat</creatorcontrib><creatorcontrib>Leaungwuttiwong, Pornsawan</creatorcontrib><creatorcontrib>Kalambaheti, Thareerat</creatorcontrib><creatorcontrib>Jampangern, Wipawee</creatorcontrib><creatorcontrib>Jittmittraphap, Akanitt</creatorcontrib><title>Cloning and application of recombinant dengue virus prM-M protein for serodiagnosis of dengue virus infection</title><title>Southeast Asian journal of tropical medicine and public health</title><addtitle>Southeast Asian J Trop Med Public Health</addtitle><description>We studied the use of the precursor to the M structural protein (prM) found only on the surface of mature dengue virus as a target protein to detect dengue virus infection. Recombinant D2-16681 prM-M protein was constructed and tested for immunogenicity with dengue and Japanese encephalitis patient sera by Western blot analysis and indirect ELISA. The sensitivity and specificity of indirect ELISA were 48.1 and 85.5%, respectively, and Western blot assay were 23.1 and 98.7%, respectively, for detection of dengue virus. Although the sensitivity of the indirect ELISA is low, the indirect ELISA using recombinant D2-16681 prM-M proteins as antigen may be used for early detection of dengue virus infection.</description><subject>Cloning, Molecular</subject><subject>Dengue - blood</subject><subject>Dengue - diagnosis</subject><subject>Dengue - immunology</subject><subject>Dengue virus</subject><subject>Dengue Virus - genetics</subject><subject>Dengue Virus - immunology</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Humans</subject><subject>Recombinant Proteins - genetics</subject><subject>Serologic Tests</subject><subject>Viral Proteins - genetics</subject><subject>Viral Proteins - immunology</subject><issn>0125-1562</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqN0DtPwzAQB_AMIFoKXwFZYmGJFMfvEVU8KrVigTny41K5SuxgJ0h8e1JRBphY7pbf_XV3Z8WywjUrMeP1orjM-VBVtMJKXhSLmnCFOcHLol93MfiwRzo4pIeh81aPPgYUW5TAxt74oMOIHIT9BOjDpymjIe3K3VzjCD6gNiaUIUXn9T7E7PNx9pf3oQV7TL0qzlvdZbg-9VXx9vjwun4uty9Pm_X9thywFGNpLbNSE8u45ZQZQ5StKFjphFBtTUE5Jx0GDYYbqLWsFcaGAuOCEeeAk1Vx95077_g-QR6b3mcLXacDxCk3mHBBJSeM_YMyKqQSgsz09g89xCmF-ZCjYgKTSqhZ3ZzUZHpwzZB8r9Nn8_Nz8gWyR32i</recordid><startdate>201303</startdate><enddate>201303</enddate><creator>Wichit, Sineewanlaya</creator><creator>Yongpradoem, Hatairat</creator><creator>Surasombatpattana, Pornapat</creator><creator>Leaungwuttiwong, Pornsawan</creator><creator>Kalambaheti, Thareerat</creator><creator>Jampangern, Wipawee</creator><creator>Jittmittraphap, Akanitt</creator><general>Central Coordinating Board, SEAMEO-TROPMED Project</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>3V.</scope><scope>7QL</scope><scope>7SS</scope><scope>7T7</scope><scope>7U7</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>BVBZV</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>P64</scope><scope>PATMY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PYCSY</scope><scope>7X8</scope><scope>F1W</scope><scope>H95</scope><scope>H97</scope><scope>L.G</scope></search><sort><creationdate>201303</creationdate><title>Cloning and application of recombinant dengue virus prM-M protein for serodiagnosis of dengue virus infection</title><author>Wichit, Sineewanlaya ; Yongpradoem, Hatairat ; Surasombatpattana, Pornapat ; Leaungwuttiwong, Pornsawan ; Kalambaheti, Thareerat ; Jampangern, Wipawee ; Jittmittraphap, Akanitt</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p187t-cc5c8a3c56c645bb39c04ec8d779f24e9dd8d1eaeb6be2a82911b4e56753dde63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Cloning, Molecular</topic><topic>Dengue - 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Recombinant D2-16681 prM-M protein was constructed and tested for immunogenicity with dengue and Japanese encephalitis patient sera by Western blot analysis and indirect ELISA. The sensitivity and specificity of indirect ELISA were 48.1 and 85.5%, respectively, and Western blot assay were 23.1 and 98.7%, respectively, for detection of dengue virus. Although the sensitivity of the indirect ELISA is low, the indirect ELISA using recombinant D2-16681 prM-M proteins as antigen may be used for early detection of dengue virus infection.</abstract><cop>Thailand</cop><pub>Central Coordinating Board, SEAMEO-TROPMED Project</pub><pmid>23691631</pmid><tpages>8</tpages></addata></record> |
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| subjects | Cloning, Molecular Dengue - blood Dengue - diagnosis Dengue - immunology Dengue virus Dengue Virus - genetics Dengue Virus - immunology Enzyme-Linked Immunosorbent Assay Humans Recombinant Proteins - genetics Serologic Tests Viral Proteins - genetics Viral Proteins - immunology |
| title | Cloning and application of recombinant dengue virus prM-M protein for serodiagnosis of dengue virus infection |
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