Influence of fluoride on activity of carboxylesterase and esterase in hemolymph of Bombyx mori
We explored the metabolic mechanism of silkworm (Bombyx mori) to NaF by using the fluoride-tolerant silkworm variety named T6 and the fluoride-sensitive variety named 734 as materials. We fed the silkworm larvae from the first day of fifth instar with mulberry leaves soaked in 50, 100, 200 and 400 m...
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Veröffentlicht in: | Sheng tai xue bao 2013, Vol.33 (4), p.1134-1141 |
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description | We explored the metabolic mechanism of silkworm (Bombyx mori) to NaF by using the fluoride-tolerant silkworm variety named T6 and the fluoride-sensitive variety named 734 as materials. We fed the silkworm larvae from the first day of fifth instar with mulberry leaves soaked in 50, 100, 200 and 400 mg/kg NaF solutions respectively. Then we examined the activities of CarE and esterase in hemolymph. Results showed that the activity of CarE was 73% to 88% in 734 strain and 72% to 81% in T6 strain compared with control groups, respectively. The CarE activity of two low concentration NaF-treated 734 groups showed extremely significant differences(P |
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We fed the silkworm larvae from the first day of fifth instar with mulberry leaves soaked in 50, 100, 200 and 400 mg/kg NaF solutions respectively. Then we examined the activities of CarE and esterase in hemolymph. Results showed that the activity of CarE was 73% to 88% in 734 strain and 72% to 81% in T6 strain compared with control groups, respectively. The CarE activity of two low concentration NaF-treated 734 groups showed extremely significant differences(P<0.01) with control group and two high concentration NaF-treated groups, while the differences among T6's all treatment groups were not significant. The esterase activity of NaF-treated groups was 89% to 97% in 734 strain and 73% to 92% in T6 strain compared with control groups, respectively. The esterase activity among all treatment groups in 734 strain show no significant differences. The esterase activity of the highest concentrations NaF-treated group showed extremely significant differences (P<0.01) compared with control group. These results suggest that the fluoride has certain inhibition to the activity of CarE and esterase in hemolymph of silkworm.</description><identifier>ISSN: 1000-0933</identifier><identifier>DOI: 10.5846/stxb201203230395</identifier><language>eng</language><subject>Bombyx mori</subject><ispartof>Sheng tai xue bao, 2013, Vol.33 (4), p.1134-1141</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c191t-6072f41d6d167bc5ef20b3352751ff2b1a2ead741b5d36672b2c4d34c471b7813</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,4010,27900,27901,27902</link.rule.ids></links><search><creatorcontrib>Mi, Z</creatorcontrib><creatorcontrib>Ruan, C</creatorcontrib><creatorcontrib>Li, J</creatorcontrib><creatorcontrib>Fu, Q</creatorcontrib><creatorcontrib>Du, W</creatorcontrib><creatorcontrib>Li, G</creatorcontrib><creatorcontrib>Long, Y</creatorcontrib><creatorcontrib>Zhu, Y</creatorcontrib><title>Influence of fluoride on activity of carboxylesterase and esterase in hemolymph of Bombyx mori</title><title>Sheng tai xue bao</title><description>We explored the metabolic mechanism of silkworm (Bombyx mori) to NaF by using the fluoride-tolerant silkworm variety named T6 and the fluoride-sensitive variety named 734 as materials. We fed the silkworm larvae from the first day of fifth instar with mulberry leaves soaked in 50, 100, 200 and 400 mg/kg NaF solutions respectively. Then we examined the activities of CarE and esterase in hemolymph. Results showed that the activity of CarE was 73% to 88% in 734 strain and 72% to 81% in T6 strain compared with control groups, respectively. The CarE activity of two low concentration NaF-treated 734 groups showed extremely significant differences(P<0.01) with control group and two high concentration NaF-treated groups, while the differences among T6's all treatment groups were not significant. The esterase activity of NaF-treated groups was 89% to 97% in 734 strain and 73% to 92% in T6 strain compared with control groups, respectively. The esterase activity among all treatment groups in 734 strain show no significant differences. The esterase activity of the highest concentrations NaF-treated group showed extremely significant differences (P<0.01) compared with control group. 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We fed the silkworm larvae from the first day of fifth instar with mulberry leaves soaked in 50, 100, 200 and 400 mg/kg NaF solutions respectively. Then we examined the activities of CarE and esterase in hemolymph. Results showed that the activity of CarE was 73% to 88% in 734 strain and 72% to 81% in T6 strain compared with control groups, respectively. The CarE activity of two low concentration NaF-treated 734 groups showed extremely significant differences(P<0.01) with control group and two high concentration NaF-treated groups, while the differences among T6's all treatment groups were not significant. The esterase activity of NaF-treated groups was 89% to 97% in 734 strain and 73% to 92% in T6 strain compared with control groups, respectively. The esterase activity among all treatment groups in 734 strain show no significant differences. The esterase activity of the highest concentrations NaF-treated group showed extremely significant differences (P<0.01) compared with control group. These results suggest that the fluoride has certain inhibition to the activity of CarE and esterase in hemolymph of silkworm.</abstract><doi>10.5846/stxb201203230395</doi><tpages>8</tpages></addata></record> |
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subjects | Bombyx mori |
title | Influence of fluoride on activity of carboxylesterase and esterase in hemolymph of Bombyx mori |
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