Antiplasmodial potential of selected medicinal plants from Eastern Ghats of South India
[Display omitted] ► Plant extracts evaluated against CQ sensitive & resistant strains of P. falciparum. ► IC50 5 & 10μg/mL in C. rotundus and Z. officinale extracts respectively. ► Resistance indices (CQ resistant Pf INDO/CQ sensitive Pf 3D7): 0.78–1.28. ► Selectivity indices (HeLa/Pf 3D7: 3...
Gespeichert in:
| Veröffentlicht in: | Experimental parasitology 2013-05, Vol.134 (1), p.26-32 |
|---|---|
| Hauptverfasser: | , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 32 |
|---|---|
| container_issue | 1 |
| container_start_page | 26 |
| container_title | Experimental parasitology |
| container_volume | 134 |
| creator | Kaushik, Naveen Kumar Bagavan, Asokan Rahuman, Abdul Abdul Mohanakrishnan, Dinesh Kamaraj, Chinnaperumal Elango, Gandhi Zahir, Abdul Abduz Sahal, Dinkar |
| description | [Display omitted]
► Plant extracts evaluated against CQ sensitive & resistant strains of P. falciparum. ► IC50 5 & 10μg/mL in C. rotundus and Z. officinale extracts respectively. ► Resistance indices (CQ resistant Pf INDO/CQ sensitive Pf 3D7): 0.78–1.28. ► Selectivity indices (HeLa/Pf 3D7: 3–15), suggesting a good margin of safety.
Malaria caused by the protozoan parasite Plasmodium falciparum, is a major health problem of the developing world. In the present study medicinal plants from Eastern Ghats of South India have been extracted with ethyl acetate and assayed for growth inhibition of asexual erythrocytic stages of chloroquine (CQ)-sensitive (3D7) and (CQ)-resistant (INDO) strains of P. falciparum in culture using the fluorescence-based SYBR Green I assay. Studied extracts showed a spectrum of antiplasmodial activities ranging from (a) very good (IC5010–15μg/mL: Ficus religiosa and Murraya koenigii); (c) moderate (IC50>15–25μg/mL: Ficus benghalensis); (d) poor activity (IC50>25–60μg/mL) and (e) inactive (IC50>60μg/mL). Resistance indices ranging from 0.78 to 1.28 suggest that some of these extracts had equal promise against the CQ resistant INDO strain of P. falciparum. Cytotoxicity assessment of the extracts against HeLa cell line using MTT assay revealed that the selectivity indices in the range of 3–15 suggesting a good margin of safety. |
| doi_str_mv | 10.1016/j.exppara.2013.01.021 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1367484194</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0014489413000313</els_id><sourcerecordid>1367484194</sourcerecordid><originalsourceid>FETCH-LOGICAL-c422t-64ea1b8febd1f706d6795017f5c81116f293b874f4497f23848048048cb679803</originalsourceid><addsrcrecordid>eNqFkMFu1DAQhi0EotvCIwA5ckmYcbyJfULVqrSVKnEoFUfLcWzqVRIHO1u1b89sd9srkiVb429-jz_GPiFUCNh821bucZ5NMhUHrCvACji-YSsEBSUXQr1lKwAUpZBKnLDTnLcAIJGL9-yE17VSisOK_T6fljAPJo-xD2Yo5rg4qtAp-iK7wdnF9cXo-mDDtL8fzLTkwqc4FhcmLy5NxeW9oRLxt3G33BfXEyV9YO-8GbL7eNzP2N2Pi1-bq_Lm5-X15vymtILzpWyEM9hJ77oefQtN37RqDdj6tZWI2Hiu6k62wtOHWs9rKSQ8L9sRKaE-Y18PuXOKf3cuL3oM2bqBxnRxlzXWTSukQCUIXR9Qm2LOyXk9pzCa9KQR9N6p3uqjU713qgE1OaW-z8cndh2JeO16kUjAlwPgTdTmTwpZ391Swpr8tzRyQ8T3A-FIxUNwSWcb3GRJayLDuo_hP0P8A-m5kto</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1367484194</pqid></control><display><type>article</type><title>Antiplasmodial potential of selected medicinal plants from Eastern Ghats of South India</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><creator>Kaushik, Naveen Kumar ; Bagavan, Asokan ; Rahuman, Abdul Abdul ; Mohanakrishnan, Dinesh ; Kamaraj, Chinnaperumal ; Elango, Gandhi ; Zahir, Abdul Abduz ; Sahal, Dinkar</creator><creatorcontrib>Kaushik, Naveen Kumar ; Bagavan, Asokan ; Rahuman, Abdul Abdul ; Mohanakrishnan, Dinesh ; Kamaraj, Chinnaperumal ; Elango, Gandhi ; Zahir, Abdul Abduz ; Sahal, Dinkar</creatorcontrib><description>[Display omitted]
► Plant extracts evaluated against CQ sensitive & resistant strains of P. falciparum. ► IC50 5 & 10μg/mL in C. rotundus and Z. officinale extracts respectively. ► Resistance indices (CQ resistant Pf INDO/CQ sensitive Pf 3D7): 0.78–1.28. ► Selectivity indices (HeLa/Pf 3D7: 3–15), suggesting a good margin of safety.
Malaria caused by the protozoan parasite Plasmodium falciparum, is a major health problem of the developing world. In the present study medicinal plants from Eastern Ghats of South India have been extracted with ethyl acetate and assayed for growth inhibition of asexual erythrocytic stages of chloroquine (CQ)-sensitive (3D7) and (CQ)-resistant (INDO) strains of P. falciparum in culture using the fluorescence-based SYBR Green I assay. Studied extracts showed a spectrum of antiplasmodial activities ranging from (a) very good (IC50<10–10μg/mL: Cyperus rotundus and Zingiber officinale); (b) good (IC50, >10–15μg/mL: Ficus religiosa and Murraya koenigii); (c) moderate (IC50>15–25μg/mL: Ficus benghalensis); (d) poor activity (IC50>25–60μg/mL) and (e) inactive (IC50>60μg/mL). Resistance indices ranging from 0.78 to 1.28 suggest that some of these extracts had equal promise against the CQ resistant INDO strain of P. falciparum. Cytotoxicity assessment of the extracts against HeLa cell line using MTT assay revealed that the selectivity indices in the range of 3–15 suggesting a good margin of safety.</description><identifier>ISSN: 0014-4894</identifier><identifier>EISSN: 1090-2449</identifier><identifier>DOI: 10.1016/j.exppara.2013.01.021</identifier><identifier>PMID: 23399920</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Antiplasmodial activity ; chloroquine ; Cyperus - chemistry ; Cyperus rotundus ; cytotoxicity ; Drug Resistance ; Eastern Ghats of South India ; ethyl acetate ; Ficus ; Ficus - chemistry ; Ficus benghalensis ; Ficus religiosa ; growth retardation ; HeLa Cells - drug effects ; Humans ; India ; Inhibitory Concentration 50 ; malaria ; margin of safety ; Medicinal plants ; Murraya ; Murraya - chemistry ; Murraya koenigii ; parasites ; parasitology ; Plant Extracts - isolation & purification ; Plant Extracts - pharmacology ; Plant Extracts - toxicity ; Plants, Medicinal - chemistry ; Plasmodium falciparum ; Plasmodium falciparum - drug effects ; Zingiber officinale ; Zingiber officinale - chemistry</subject><ispartof>Experimental parasitology, 2013-05, Vol.134 (1), p.26-32</ispartof><rights>2013 Elsevier Inc.</rights><rights>Copyright © 2013 Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c422t-64ea1b8febd1f706d6795017f5c81116f293b874f4497f23848048048cb679803</citedby><cites>FETCH-LOGICAL-c422t-64ea1b8febd1f706d6795017f5c81116f293b874f4497f23848048048cb679803</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0014489413000313$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23399920$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kaushik, Naveen Kumar</creatorcontrib><creatorcontrib>Bagavan, Asokan</creatorcontrib><creatorcontrib>Rahuman, Abdul Abdul</creatorcontrib><creatorcontrib>Mohanakrishnan, Dinesh</creatorcontrib><creatorcontrib>Kamaraj, Chinnaperumal</creatorcontrib><creatorcontrib>Elango, Gandhi</creatorcontrib><creatorcontrib>Zahir, Abdul Abduz</creatorcontrib><creatorcontrib>Sahal, Dinkar</creatorcontrib><title>Antiplasmodial potential of selected medicinal plants from Eastern Ghats of South India</title><title>Experimental parasitology</title><addtitle>Exp Parasitol</addtitle><description>[Display omitted]
► Plant extracts evaluated against CQ sensitive & resistant strains of P. falciparum. ► IC50 5 & 10μg/mL in C. rotundus and Z. officinale extracts respectively. ► Resistance indices (CQ resistant Pf INDO/CQ sensitive Pf 3D7): 0.78–1.28. ► Selectivity indices (HeLa/Pf 3D7: 3–15), suggesting a good margin of safety.
Malaria caused by the protozoan parasite Plasmodium falciparum, is a major health problem of the developing world. In the present study medicinal plants from Eastern Ghats of South India have been extracted with ethyl acetate and assayed for growth inhibition of asexual erythrocytic stages of chloroquine (CQ)-sensitive (3D7) and (CQ)-resistant (INDO) strains of P. falciparum in culture using the fluorescence-based SYBR Green I assay. Studied extracts showed a spectrum of antiplasmodial activities ranging from (a) very good (IC50<10–10μg/mL: Cyperus rotundus and Zingiber officinale); (b) good (IC50, >10–15μg/mL: Ficus religiosa and Murraya koenigii); (c) moderate (IC50>15–25μg/mL: Ficus benghalensis); (d) poor activity (IC50>25–60μg/mL) and (e) inactive (IC50>60μg/mL). Resistance indices ranging from 0.78 to 1.28 suggest that some of these extracts had equal promise against the CQ resistant INDO strain of P. falciparum. Cytotoxicity assessment of the extracts against HeLa cell line using MTT assay revealed that the selectivity indices in the range of 3–15 suggesting a good margin of safety.</description><subject>Antiplasmodial activity</subject><subject>chloroquine</subject><subject>Cyperus - chemistry</subject><subject>Cyperus rotundus</subject><subject>cytotoxicity</subject><subject>Drug Resistance</subject><subject>Eastern Ghats of South India</subject><subject>ethyl acetate</subject><subject>Ficus</subject><subject>Ficus - chemistry</subject><subject>Ficus benghalensis</subject><subject>Ficus religiosa</subject><subject>growth retardation</subject><subject>HeLa Cells - drug effects</subject><subject>Humans</subject><subject>India</subject><subject>Inhibitory Concentration 50</subject><subject>malaria</subject><subject>margin of safety</subject><subject>Medicinal plants</subject><subject>Murraya</subject><subject>Murraya - chemistry</subject><subject>Murraya koenigii</subject><subject>parasites</subject><subject>parasitology</subject><subject>Plant Extracts - isolation & purification</subject><subject>Plant Extracts - pharmacology</subject><subject>Plant Extracts - toxicity</subject><subject>Plants, Medicinal - chemistry</subject><subject>Plasmodium falciparum</subject><subject>Plasmodium falciparum - drug effects</subject><subject>Zingiber officinale</subject><subject>Zingiber officinale - chemistry</subject><issn>0014-4894</issn><issn>1090-2449</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkMFu1DAQhi0EotvCIwA5ckmYcbyJfULVqrSVKnEoFUfLcWzqVRIHO1u1b89sd9srkiVb429-jz_GPiFUCNh821bucZ5NMhUHrCvACji-YSsEBSUXQr1lKwAUpZBKnLDTnLcAIJGL9-yE17VSisOK_T6fljAPJo-xD2Yo5rg4qtAp-iK7wdnF9cXo-mDDtL8fzLTkwqc4FhcmLy5NxeW9oRLxt3G33BfXEyV9YO-8GbL7eNzP2N2Pi1-bq_Lm5-X15vymtILzpWyEM9hJ77oefQtN37RqDdj6tZWI2Hiu6k62wtOHWs9rKSQ8L9sRKaE-Y18PuXOKf3cuL3oM2bqBxnRxlzXWTSukQCUIXR9Qm2LOyXk9pzCa9KQR9N6p3uqjU713qgE1OaW-z8cndh2JeO16kUjAlwPgTdTmTwpZ391Swpr8tzRyQ8T3A-FIxUNwSWcb3GRJayLDuo_hP0P8A-m5kto</recordid><startdate>20130501</startdate><enddate>20130501</enddate><creator>Kaushik, Naveen Kumar</creator><creator>Bagavan, Asokan</creator><creator>Rahuman, Abdul Abdul</creator><creator>Mohanakrishnan, Dinesh</creator><creator>Kamaraj, Chinnaperumal</creator><creator>Elango, Gandhi</creator><creator>Zahir, Abdul Abduz</creator><creator>Sahal, Dinkar</creator><general>Elsevier Inc</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>C1K</scope><scope>F1W</scope><scope>H95</scope><scope>H97</scope><scope>L.G</scope><scope>M7N</scope></search><sort><creationdate>20130501</creationdate><title>Antiplasmodial potential of selected medicinal plants from Eastern Ghats of South India</title><author>Kaushik, Naveen Kumar ; Bagavan, Asokan ; Rahuman, Abdul Abdul ; Mohanakrishnan, Dinesh ; Kamaraj, Chinnaperumal ; Elango, Gandhi ; Zahir, Abdul Abduz ; Sahal, Dinkar</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c422t-64ea1b8febd1f706d6795017f5c81116f293b874f4497f23848048048cb679803</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Antiplasmodial activity</topic><topic>chloroquine</topic><topic>Cyperus - chemistry</topic><topic>Cyperus rotundus</topic><topic>cytotoxicity</topic><topic>Drug Resistance</topic><topic>Eastern Ghats of South India</topic><topic>ethyl acetate</topic><topic>Ficus</topic><topic>Ficus - chemistry</topic><topic>Ficus benghalensis</topic><topic>Ficus religiosa</topic><topic>growth retardation</topic><topic>HeLa Cells - drug effects</topic><topic>Humans</topic><topic>India</topic><topic>Inhibitory Concentration 50</topic><topic>malaria</topic><topic>margin of safety</topic><topic>Medicinal plants</topic><topic>Murraya</topic><topic>Murraya - chemistry</topic><topic>Murraya koenigii</topic><topic>parasites</topic><topic>parasitology</topic><topic>Plant Extracts - isolation & purification</topic><topic>Plant Extracts - pharmacology</topic><topic>Plant Extracts - toxicity</topic><topic>Plants, Medicinal - chemistry</topic><topic>Plasmodium falciparum</topic><topic>Plasmodium falciparum - drug effects</topic><topic>Zingiber officinale</topic><topic>Zingiber officinale - chemistry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kaushik, Naveen Kumar</creatorcontrib><creatorcontrib>Bagavan, Asokan</creatorcontrib><creatorcontrib>Rahuman, Abdul Abdul</creatorcontrib><creatorcontrib>Mohanakrishnan, Dinesh</creatorcontrib><creatorcontrib>Kamaraj, Chinnaperumal</creatorcontrib><creatorcontrib>Elango, Gandhi</creatorcontrib><creatorcontrib>Zahir, Abdul Abduz</creatorcontrib><creatorcontrib>Sahal, Dinkar</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 3: Aquatic Pollution & Environmental Quality</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><jtitle>Experimental parasitology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kaushik, Naveen Kumar</au><au>Bagavan, Asokan</au><au>Rahuman, Abdul Abdul</au><au>Mohanakrishnan, Dinesh</au><au>Kamaraj, Chinnaperumal</au><au>Elango, Gandhi</au><au>Zahir, Abdul Abduz</au><au>Sahal, Dinkar</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Antiplasmodial potential of selected medicinal plants from Eastern Ghats of South India</atitle><jtitle>Experimental parasitology</jtitle><addtitle>Exp Parasitol</addtitle><date>2013-05-01</date><risdate>2013</risdate><volume>134</volume><issue>1</issue><spage>26</spage><epage>32</epage><pages>26-32</pages><issn>0014-4894</issn><eissn>1090-2449</eissn><abstract>[Display omitted]
► Plant extracts evaluated against CQ sensitive & resistant strains of P. falciparum. ► IC50 5 & 10μg/mL in C. rotundus and Z. officinale extracts respectively. ► Resistance indices (CQ resistant Pf INDO/CQ sensitive Pf 3D7): 0.78–1.28. ► Selectivity indices (HeLa/Pf 3D7: 3–15), suggesting a good margin of safety.
Malaria caused by the protozoan parasite Plasmodium falciparum, is a major health problem of the developing world. In the present study medicinal plants from Eastern Ghats of South India have been extracted with ethyl acetate and assayed for growth inhibition of asexual erythrocytic stages of chloroquine (CQ)-sensitive (3D7) and (CQ)-resistant (INDO) strains of P. falciparum in culture using the fluorescence-based SYBR Green I assay. Studied extracts showed a spectrum of antiplasmodial activities ranging from (a) very good (IC50<10–10μg/mL: Cyperus rotundus and Zingiber officinale); (b) good (IC50, >10–15μg/mL: Ficus religiosa and Murraya koenigii); (c) moderate (IC50>15–25μg/mL: Ficus benghalensis); (d) poor activity (IC50>25–60μg/mL) and (e) inactive (IC50>60μg/mL). Resistance indices ranging from 0.78 to 1.28 suggest that some of these extracts had equal promise against the CQ resistant INDO strain of P. falciparum. Cytotoxicity assessment of the extracts against HeLa cell line using MTT assay revealed that the selectivity indices in the range of 3–15 suggesting a good margin of safety.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>23399920</pmid><doi>10.1016/j.exppara.2013.01.021</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0014-4894 |
| ispartof | Experimental parasitology, 2013-05, Vol.134 (1), p.26-32 |
| issn | 0014-4894 1090-2449 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1367484194 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Antiplasmodial activity chloroquine Cyperus - chemistry Cyperus rotundus cytotoxicity Drug Resistance Eastern Ghats of South India ethyl acetate Ficus Ficus - chemistry Ficus benghalensis Ficus religiosa growth retardation HeLa Cells - drug effects Humans India Inhibitory Concentration 50 malaria margin of safety Medicinal plants Murraya Murraya - chemistry Murraya koenigii parasites parasitology Plant Extracts - isolation & purification Plant Extracts - pharmacology Plant Extracts - toxicity Plants, Medicinal - chemistry Plasmodium falciparum Plasmodium falciparum - drug effects Zingiber officinale Zingiber officinale - chemistry |
| title | Antiplasmodial potential of selected medicinal plants from Eastern Ghats of South India |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-03T16%3A25%3A46IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Antiplasmodial%20potential%20of%20selected%20medicinal%20plants%20from%20Eastern%20Ghats%20of%20South%20India&rft.jtitle=Experimental%20parasitology&rft.au=Kaushik,%20Naveen%20Kumar&rft.date=2013-05-01&rft.volume=134&rft.issue=1&rft.spage=26&rft.epage=32&rft.pages=26-32&rft.issn=0014-4894&rft.eissn=1090-2449&rft_id=info:doi/10.1016/j.exppara.2013.01.021&rft_dat=%3Cproquest_cross%3E1367484194%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1367484194&rft_id=info:pmid/23399920&rft_els_id=S0014489413000313&rfr_iscdi=true |