Effects of formaldehyde exposure on the extractability of DNA from proteins in the rat nasal mucosa
Reaction of an homogenate of the rat nasal mucosa with formaldehyde (CH 2O) followed by solubilization and extraction in a strongly denaturing aqueous-immiscible organic solvent mixture decreased the quantity of nucleic acids that remained in aqueous solution in comparison with a control homogenate...
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| Veröffentlicht in: | Toxicology and applied pharmacology 1983-01, Vol.70 (1), p.121-132 |
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| creator | Casanova-Schmitz, Mercedes Heck, Henry D'A. |
| description | Reaction of an homogenate of the rat nasal mucosa with formaldehyde (CH
2O) followed by solubilization and extraction in a strongly denaturing aqueous-immiscible organic solvent mixture decreased the quantity of nucleic acids that remained in aqueous solution in comparison with a control homogenate untreated with CH
2O. The absent DNA and RNA were located in the interface between the aqueous and organic phases, from which they could be recovered only after enzymatic proteolysis. It is concluded that the interfacial nucleic acids were cross-linked to proteins by CH
2O. The concentration of cross-links was estimated with
14CH
2O; under conditions that rendered 48% of the DNA nonextractable from proteins, there was less than one cross-link per 28,000 nucleotide residues. Exposure of rats to airborne CH
2O at concentrations of 0, 2, 6, 15, and 30 ppm (6 hr/day for 2 days) resulted in a statistically significant increase in the percentage of the total DNA from the respiratory mucosa that was located in the interface at concentrations of 6 ppm and higher. However, the percentage of DNA from the olfactory mucosa located in the interface was not increased by CH
2O exposure. Analysis of nasal mucosal DNA by ultracentrifugation in CsCl density gradients provided no evidence of a change in the buoyant density of the DNA caused by reaction with CH
2O. The results indicate that CH
2O may induce DNA-protein cross-links in the respiratory but not in the olfactory mucosa at concentrations equal to or greater than 6 ppm. |
| doi_str_mv | 10.1016/0041-008X(83)90185-0 |
| format | Article |
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2O) followed by solubilization and extraction in a strongly denaturing aqueous-immiscible organic solvent mixture decreased the quantity of nucleic acids that remained in aqueous solution in comparison with a control homogenate untreated with CH
2O. The absent DNA and RNA were located in the interface between the aqueous and organic phases, from which they could be recovered only after enzymatic proteolysis. It is concluded that the interfacial nucleic acids were cross-linked to proteins by CH
2O. The concentration of cross-links was estimated with
14CH
2O; under conditions that rendered 48% of the DNA nonextractable from proteins, there was less than one cross-link per 28,000 nucleotide residues. Exposure of rats to airborne CH
2O at concentrations of 0, 2, 6, 15, and 30 ppm (6 hr/day for 2 days) resulted in a statistically significant increase in the percentage of the total DNA from the respiratory mucosa that was located in the interface at concentrations of 6 ppm and higher. However, the percentage of DNA from the olfactory mucosa located in the interface was not increased by CH
2O exposure. Analysis of nasal mucosal DNA by ultracentrifugation in CsCl density gradients provided no evidence of a change in the buoyant density of the DNA caused by reaction with CH
2O. The results indicate that CH
2O may induce DNA-protein cross-links in the respiratory but not in the olfactory mucosa at concentrations equal to or greater than 6 ppm.</description><identifier>ISSN: 0041-008X</identifier><identifier>EISSN: 1096-0333</identifier><identifier>DOI: 10.1016/0041-008X(83)90185-0</identifier><identifier>PMID: 6193611</identifier><identifier>CODEN: TXAPA9</identifier><language>eng</language><publisher>San Diego, CA: Elsevier Inc</publisher><subject>Animals ; Biological and medical sciences ; Carcinogenesis, carcinogens and anticarcinogens ; Centrifugation, Isopycnic ; Chemical agents ; DNA - isolation & purification ; Formaldehyde - toxicity ; Male ; Medical sciences ; Nasal Mucosa - analysis ; Nasal Mucosa - drug effects ; Proteins - analysis ; Rats ; RNA - isolation & purification ; Tumors</subject><ispartof>Toxicology and applied pharmacology, 1983-01, Vol.70 (1), p.121-132</ispartof><rights>1983</rights><rights>1984 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c417t-cd00076ee44c1df42cdc0b54363ec9cc0f5e3b24cacbd82ffd2ad443b13036803</citedby><cites>FETCH-LOGICAL-c417t-cd00076ee44c1df42cdc0b54363ec9cc0f5e3b24cacbd82ffd2ad443b13036803</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0041-008X(83)90185-0$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,777,781,3537,27905,27906,45976</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=9376566$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/6193611$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Casanova-Schmitz, Mercedes</creatorcontrib><creatorcontrib>Heck, Henry D'A.</creatorcontrib><title>Effects of formaldehyde exposure on the extractability of DNA from proteins in the rat nasal mucosa</title><title>Toxicology and applied pharmacology</title><addtitle>Toxicol Appl Pharmacol</addtitle><description>Reaction of an homogenate of the rat nasal mucosa with formaldehyde (CH
2O) followed by solubilization and extraction in a strongly denaturing aqueous-immiscible organic solvent mixture decreased the quantity of nucleic acids that remained in aqueous solution in comparison with a control homogenate untreated with CH
2O. The absent DNA and RNA were located in the interface between the aqueous and organic phases, from which they could be recovered only after enzymatic proteolysis. It is concluded that the interfacial nucleic acids were cross-linked to proteins by CH
2O. The concentration of cross-links was estimated with
14CH
2O; under conditions that rendered 48% of the DNA nonextractable from proteins, there was less than one cross-link per 28,000 nucleotide residues. Exposure of rats to airborne CH
2O at concentrations of 0, 2, 6, 15, and 30 ppm (6 hr/day for 2 days) resulted in a statistically significant increase in the percentage of the total DNA from the respiratory mucosa that was located in the interface at concentrations of 6 ppm and higher. However, the percentage of DNA from the olfactory mucosa located in the interface was not increased by CH
2O exposure. Analysis of nasal mucosal DNA by ultracentrifugation in CsCl density gradients provided no evidence of a change in the buoyant density of the DNA caused by reaction with CH
2O. The results indicate that CH
2O may induce DNA-protein cross-links in the respiratory but not in the olfactory mucosa at concentrations equal to or greater than 6 ppm.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Carcinogenesis, carcinogens and anticarcinogens</subject><subject>Centrifugation, Isopycnic</subject><subject>Chemical agents</subject><subject>DNA - isolation & purification</subject><subject>Formaldehyde - toxicity</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Nasal Mucosa - analysis</subject><subject>Nasal Mucosa - drug effects</subject><subject>Proteins - analysis</subject><subject>Rats</subject><subject>RNA - isolation & purification</subject><subject>Tumors</subject><issn>0041-008X</issn><issn>1096-0333</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1983</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kcFu1DAQhi1EVZbCG4DkA0JwCIxjr5NcKlWltJUquIDEzXLGY9UoiRfbQezbk7CrPXIajebzr1-fGXsl4IMAoT8CKFEBtD_etfJ9B6LdVvCEbQR0ugIp5VO2OSHP2POcfwJAp5Q4Z-dadFILsWF44z1hyTx67mMa7eDoce-I059dzHMiHideHte9JIvF9mEIZb_in75ccZ_iyHcpFgpT5uGAJlv4ZLMd-DhjzPYFO_N2yPTyOC_Y9883367vqoevt_fXVw8VKtGUCt3Sr9FESqFwXtXoEPqtkloSdojgtyT7WqHF3rW19662TinZCwlStyAv2NtD7lLo10y5mDFkpGGwE8U5GyF1A13dLKA6gJhizom82aUw2rQ3Aszq1qzizCrOtNL8c2vW_NfH_LkfyZ0eHWUu9zfHu81oB5_shCGfsE42eqv1gl0eMFpc_A6UTMZAE5ILafkK42L4f4-_sAyWew</recordid><startdate>19830101</startdate><enddate>19830101</enddate><creator>Casanova-Schmitz, Mercedes</creator><creator>Heck, Henry D'A.</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>7U7</scope><scope>C1K</scope></search><sort><creationdate>19830101</creationdate><title>Effects of formaldehyde exposure on the extractability of DNA from proteins in the rat nasal mucosa</title><author>Casanova-Schmitz, Mercedes ; Heck, Henry D'A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c417t-cd00076ee44c1df42cdc0b54363ec9cc0f5e3b24cacbd82ffd2ad443b13036803</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1983</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Carcinogenesis, carcinogens and anticarcinogens</topic><topic>Centrifugation, Isopycnic</topic><topic>Chemical agents</topic><topic>DNA - isolation & purification</topic><topic>Formaldehyde - toxicity</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Nasal Mucosa - analysis</topic><topic>Nasal Mucosa - drug effects</topic><topic>Proteins - analysis</topic><topic>Rats</topic><topic>RNA - isolation & purification</topic><topic>Tumors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Casanova-Schmitz, Mercedes</creatorcontrib><creatorcontrib>Heck, Henry D'A.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><jtitle>Toxicology and applied pharmacology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Casanova-Schmitz, Mercedes</au><au>Heck, Henry D'A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of formaldehyde exposure on the extractability of DNA from proteins in the rat nasal mucosa</atitle><jtitle>Toxicology and applied pharmacology</jtitle><addtitle>Toxicol Appl Pharmacol</addtitle><date>1983-01-01</date><risdate>1983</risdate><volume>70</volume><issue>1</issue><spage>121</spage><epage>132</epage><pages>121-132</pages><issn>0041-008X</issn><eissn>1096-0333</eissn><coden>TXAPA9</coden><abstract>Reaction of an homogenate of the rat nasal mucosa with formaldehyde (CH
2O) followed by solubilization and extraction in a strongly denaturing aqueous-immiscible organic solvent mixture decreased the quantity of nucleic acids that remained in aqueous solution in comparison with a control homogenate untreated with CH
2O. The absent DNA and RNA were located in the interface between the aqueous and organic phases, from which they could be recovered only after enzymatic proteolysis. It is concluded that the interfacial nucleic acids were cross-linked to proteins by CH
2O. The concentration of cross-links was estimated with
14CH
2O; under conditions that rendered 48% of the DNA nonextractable from proteins, there was less than one cross-link per 28,000 nucleotide residues. Exposure of rats to airborne CH
2O at concentrations of 0, 2, 6, 15, and 30 ppm (6 hr/day for 2 days) resulted in a statistically significant increase in the percentage of the total DNA from the respiratory mucosa that was located in the interface at concentrations of 6 ppm and higher. However, the percentage of DNA from the olfactory mucosa located in the interface was not increased by CH
2O exposure. Analysis of nasal mucosal DNA by ultracentrifugation in CsCl density gradients provided no evidence of a change in the buoyant density of the DNA caused by reaction with CH
2O. The results indicate that CH
2O may induce DNA-protein cross-links in the respiratory but not in the olfactory mucosa at concentrations equal to or greater than 6 ppm.</abstract><cop>San Diego, CA</cop><pub>Elsevier Inc</pub><pmid>6193611</pmid><doi>10.1016/0041-008X(83)90185-0</doi><tpages>12</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0041-008X |
| ispartof | Toxicology and applied pharmacology, 1983-01, Vol.70 (1), p.121-132 |
| issn | 0041-008X 1096-0333 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_13670927 |
| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Animals Biological and medical sciences Carcinogenesis, carcinogens and anticarcinogens Centrifugation, Isopycnic Chemical agents DNA - isolation & purification Formaldehyde - toxicity Male Medical sciences Nasal Mucosa - analysis Nasal Mucosa - drug effects Proteins - analysis Rats RNA - isolation & purification Tumors |
| title | Effects of formaldehyde exposure on the extractability of DNA from proteins in the rat nasal mucosa |
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