LC-MS/MS determination and pharmacokinetic study of lacidipine in human plasma
ABSTRACT A robust, specific and fully validated LC‐MS/MS method as per general practices of industry has been developed for estimation of lacidipine (LAC) with 100 μL of human plasma using lacidipine‐13C8 as an internal standard (IS). The API‐4000 LC‐MS/MS was operated under the multiple reaction‐mo...
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| Veröffentlicht in: | Biomedical chromatography 2013-07, Vol.27 (7), p.838-845 |
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| creator | Chatki, Pankaj Kisan Hotha, Kishore Kumar Kolagatla, Pandu Ranga Reddy Bharathi, D. Vijaya Venkateswarulu, V. |
| description | ABSTRACT
A robust, specific and fully validated LC‐MS/MS method as per general practices of industry has been developed for estimation of lacidipine (LAC) with 100 μL of human plasma using lacidipine‐13C8 as an internal standard (IS). The API‐4000 LC‐MS/MS was operated under the multiple reaction‐monitoring mode. A simple liquid–liquid extraction process was used to extract LAC and IS from human plasma. The total run time was 3.0 min and the elution of LAC and IS occurred at 1.96 and 1.97 min; this was achieved with a mobile phase consisting of 5 mm ammonium acetate buffer–acetontrile (15:85 v/v) at a flow rate of 0.60 mL/min on a Zorbax SB C18 (50 × 4.6 mm, 5 µm) column. A linear response function was established for the range of concentrations 50–15,000 pg/mL (r > 0.998) for LAC. The current developed method has negligible matrix effect and is free from unwanted adducts and clusters which are formed owing to system such as solvent or mobile phase. The developed assay method was applied to an oral pharmacokinetic study in humans and successfully characterized the pharmacokinetic data up to 72 h. Copyright © 2013 John Wiley & Sons, Ltd. |
| doi_str_mv | 10.1002/bmc.2868 |
| format | Article |
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A robust, specific and fully validated LC‐MS/MS method as per general practices of industry has been developed for estimation of lacidipine (LAC) with 100 μL of human plasma using lacidipine‐13C8 as an internal standard (IS). The API‐4000 LC‐MS/MS was operated under the multiple reaction‐monitoring mode. A simple liquid–liquid extraction process was used to extract LAC and IS from human plasma. The total run time was 3.0 min and the elution of LAC and IS occurred at 1.96 and 1.97 min; this was achieved with a mobile phase consisting of 5 mm ammonium acetate buffer–acetontrile (15:85 v/v) at a flow rate of 0.60 mL/min on a Zorbax SB C18 (50 × 4.6 mm, 5 µm) column. A linear response function was established for the range of concentrations 50–15,000 pg/mL (r > 0.998) for LAC. The current developed method has negligible matrix effect and is free from unwanted adducts and clusters which are formed owing to system such as solvent or mobile phase. The developed assay method was applied to an oral pharmacokinetic study in humans and successfully characterized the pharmacokinetic data up to 72 h. Copyright © 2013 John Wiley & Sons, Ltd.</description><identifier>ISSN: 0269-3879</identifier><identifier>EISSN: 1099-0801</identifier><identifier>DOI: 10.1002/bmc.2868</identifier><identifier>PMID: 23460049</identifier><language>eng</language><publisher>England: Blackwell Publishing Ltd</publisher><subject>Adolescent ; Adult ; Chromatography, High Pressure Liquid - methods ; Dihydropyridines - blood ; Dihydropyridines - chemistry ; Dihydropyridines - pharmacokinetics ; Drug Stability ; human plasma ; Humans ; lacidipine ; LC-MS/MS ; Male ; method validation ; Middle Aged ; pharmacokinetics ; Reproducibility of Results ; Spectrometry, Mass, Electrospray Ionization ; Tandem Mass Spectrometry - methods</subject><ispartof>Biomedical chromatography, 2013-07, Vol.27 (7), p.838-845</ispartof><rights>Copyright © 2013 John Wiley & Sons, Ltd.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3598-8930cbd25cc6c31c2267c88c916f7cef424c28f80a21936eed76b9da787dfe4e3</citedby><cites>FETCH-LOGICAL-c3598-8930cbd25cc6c31c2267c88c916f7cef424c28f80a21936eed76b9da787dfe4e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fbmc.2868$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fbmc.2868$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23460049$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chatki, Pankaj Kisan</creatorcontrib><creatorcontrib>Hotha, Kishore Kumar</creatorcontrib><creatorcontrib>Kolagatla, Pandu Ranga Reddy</creatorcontrib><creatorcontrib>Bharathi, D. Vijaya</creatorcontrib><creatorcontrib>Venkateswarulu, V.</creatorcontrib><title>LC-MS/MS determination and pharmacokinetic study of lacidipine in human plasma</title><title>Biomedical chromatography</title><addtitle>Biomed. Chromatogr</addtitle><description>ABSTRACT
A robust, specific and fully validated LC‐MS/MS method as per general practices of industry has been developed for estimation of lacidipine (LAC) with 100 μL of human plasma using lacidipine‐13C8 as an internal standard (IS). The API‐4000 LC‐MS/MS was operated under the multiple reaction‐monitoring mode. A simple liquid–liquid extraction process was used to extract LAC and IS from human plasma. The total run time was 3.0 min and the elution of LAC and IS occurred at 1.96 and 1.97 min; this was achieved with a mobile phase consisting of 5 mm ammonium acetate buffer–acetontrile (15:85 v/v) at a flow rate of 0.60 mL/min on a Zorbax SB C18 (50 × 4.6 mm, 5 µm) column. A linear response function was established for the range of concentrations 50–15,000 pg/mL (r > 0.998) for LAC. The current developed method has negligible matrix effect and is free from unwanted adducts and clusters which are formed owing to system such as solvent or mobile phase. The developed assay method was applied to an oral pharmacokinetic study in humans and successfully characterized the pharmacokinetic data up to 72 h. Copyright © 2013 John Wiley & Sons, Ltd.</description><subject>Adolescent</subject><subject>Adult</subject><subject>Chromatography, High Pressure Liquid - methods</subject><subject>Dihydropyridines - blood</subject><subject>Dihydropyridines - chemistry</subject><subject>Dihydropyridines - pharmacokinetics</subject><subject>Drug Stability</subject><subject>human plasma</subject><subject>Humans</subject><subject>lacidipine</subject><subject>LC-MS/MS</subject><subject>Male</subject><subject>method validation</subject><subject>Middle Aged</subject><subject>pharmacokinetics</subject><subject>Reproducibility of Results</subject><subject>Spectrometry, Mass, Electrospray Ionization</subject><subject>Tandem Mass Spectrometry - methods</subject><issn>0269-3879</issn><issn>1099-0801</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp10EtLAzEUhuEgiq0X8BdIlm6mzWWay1JHbYVW0VYKbkKaZGjs3JzMoP33jlh15erA4eFbvACcYTTACJHhKjcDIpjYA32MpIyQQHgf9BFhMqKCyx44CuEVISQZ4YegR2jMEIplH9xPk2g2H87m0LrG1bkvdOPLAurCwmqt61ybcuML13gDQ9PaLSxTmGnjra-6N_QFXLe5LmCV6ZDrE3CQ6iy40909Bs-3N4tkEk0fxnfJ5TQydCRFJCRFZmXJyBhmKDaEMG6EMBKzlBuXxiQ2RKQCaYIlZc5ZzlbSai64TV3s6DG4-N6t6vKtdaFRuQ_GZZkuXNkGhSljgiCC-R81dRlC7VJV1T7X9VZhpL7qqa6e-qrX0fPdarvKnf2FP7k6EH2Dd5-57b9D6mqW7AZ33ofGffx6XW8U45SP1PJ-rEYvk8frRbJUT_QTEp-Hew</recordid><startdate>201307</startdate><enddate>201307</enddate><creator>Chatki, Pankaj Kisan</creator><creator>Hotha, Kishore Kumar</creator><creator>Kolagatla, Pandu Ranga Reddy</creator><creator>Bharathi, D. Vijaya</creator><creator>Venkateswarulu, V.</creator><general>Blackwell Publishing Ltd</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>201307</creationdate><title>LC-MS/MS determination and pharmacokinetic study of lacidipine in human plasma</title><author>Chatki, Pankaj Kisan ; Hotha, Kishore Kumar ; Kolagatla, Pandu Ranga Reddy ; Bharathi, D. Vijaya ; Venkateswarulu, V.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3598-8930cbd25cc6c31c2267c88c916f7cef424c28f80a21936eed76b9da787dfe4e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Adolescent</topic><topic>Adult</topic><topic>Chromatography, High Pressure Liquid - methods</topic><topic>Dihydropyridines - blood</topic><topic>Dihydropyridines - chemistry</topic><topic>Dihydropyridines - pharmacokinetics</topic><topic>Drug Stability</topic><topic>human plasma</topic><topic>Humans</topic><topic>lacidipine</topic><topic>LC-MS/MS</topic><topic>Male</topic><topic>method validation</topic><topic>Middle Aged</topic><topic>pharmacokinetics</topic><topic>Reproducibility of Results</topic><topic>Spectrometry, Mass, Electrospray Ionization</topic><topic>Tandem Mass Spectrometry - methods</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chatki, Pankaj Kisan</creatorcontrib><creatorcontrib>Hotha, Kishore Kumar</creatorcontrib><creatorcontrib>Kolagatla, Pandu Ranga Reddy</creatorcontrib><creatorcontrib>Bharathi, D. Vijaya</creatorcontrib><creatorcontrib>Venkateswarulu, V.</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Biomedical chromatography</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chatki, Pankaj Kisan</au><au>Hotha, Kishore Kumar</au><au>Kolagatla, Pandu Ranga Reddy</au><au>Bharathi, D. Vijaya</au><au>Venkateswarulu, V.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>LC-MS/MS determination and pharmacokinetic study of lacidipine in human plasma</atitle><jtitle>Biomedical chromatography</jtitle><addtitle>Biomed. Chromatogr</addtitle><date>2013-07</date><risdate>2013</risdate><volume>27</volume><issue>7</issue><spage>838</spage><epage>845</epage><pages>838-845</pages><issn>0269-3879</issn><eissn>1099-0801</eissn><abstract>ABSTRACT
A robust, specific and fully validated LC‐MS/MS method as per general practices of industry has been developed for estimation of lacidipine (LAC) with 100 μL of human plasma using lacidipine‐13C8 as an internal standard (IS). The API‐4000 LC‐MS/MS was operated under the multiple reaction‐monitoring mode. A simple liquid–liquid extraction process was used to extract LAC and IS from human plasma. The total run time was 3.0 min and the elution of LAC and IS occurred at 1.96 and 1.97 min; this was achieved with a mobile phase consisting of 5 mm ammonium acetate buffer–acetontrile (15:85 v/v) at a flow rate of 0.60 mL/min on a Zorbax SB C18 (50 × 4.6 mm, 5 µm) column. A linear response function was established for the range of concentrations 50–15,000 pg/mL (r > 0.998) for LAC. The current developed method has negligible matrix effect and is free from unwanted adducts and clusters which are formed owing to system such as solvent or mobile phase. The developed assay method was applied to an oral pharmacokinetic study in humans and successfully characterized the pharmacokinetic data up to 72 h. Copyright © 2013 John Wiley & Sons, Ltd.</abstract><cop>England</cop><pub>Blackwell Publishing Ltd</pub><pmid>23460049</pmid><doi>10.1002/bmc.2868</doi><tpages>8</tpages></addata></record> |
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| subjects | Adolescent Adult Chromatography, High Pressure Liquid - methods Dihydropyridines - blood Dihydropyridines - chemistry Dihydropyridines - pharmacokinetics Drug Stability human plasma Humans lacidipine LC-MS/MS Male method validation Middle Aged pharmacokinetics Reproducibility of Results Spectrometry, Mass, Electrospray Ionization Tandem Mass Spectrometry - methods |
| title | LC-MS/MS determination and pharmacokinetic study of lacidipine in human plasma |
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