Glaciimonas singularis sp. nov., isolated from a uranium mine wastewater treatment plant
A bacterial strain, A2-57T, recovered from a water sample collected in a uranium mine was taxonomically studied in detail. This strain was a Gram-reaction-negative, rod-shaped bacterium that grew optimally at 25 °C and at pH 6.0–7.0 and had a DNA G+C content of 55.0 mol%. Ubiquinone 8 (UQ-8) was the...
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| Veröffentlicht in: | International journal of systematic and evolutionary microbiology 2013-06, Vol.63 (Pt 6), p.2344-2350 |
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| container_title | International journal of systematic and evolutionary microbiology |
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| creator | Chung, Ana Paula Tiago, Igor Nobre, M. Fernanda Veríssimo, António Morais, Paula V |
| description | A bacterial strain, A2-57T, recovered from a water sample collected in a uranium mine was taxonomically studied in detail. This strain was a Gram-reaction-negative, rod-shaped bacterium that grew optimally at 25 °C and at pH 6.0–7.0 and had a DNA G+C content of 55.0 mol%. Ubiquinone 8 (UQ-8) was the predominant respiratory quinone and the major fatty acids were C16 : 0, C17 : 0 cyclo, summed feature 3 (C16 : 1ω6c and/or ω7c and/or C15 : 0 iso 2-OH) and C18 : 1ω7c. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain A2-57T belonged to the family Oxalobacteraceae and formed a distinct branch with Glaciimonas immobilis Cr9-30T. Strain A2-57T shared approximately 97.3 % 16S rRNA sequence similarity with G. immobilis Cr9-30T and also showed high sequence similarity with members of the genera Herbaspirillum (96.3–97.0 %) and Collimonas (96.2–97.0 %). Although phylogenetically closely related to the type strain of G. immobilis, the low level of DNA–DNA hybridization between the two strains (21.6 %) and several physiological and biochemical properties indicated that the novel strain could be clearly distinguished from G. immobilis LMG 25547T. Therefore, it is concluded that strain A2-57T represents a novel species of the genus Glaciimonas, for which the name Glaciimonas singularis sp. nov. is proposed. The type strain is A2-57T ( = CIP 110539T = LMG 27070T). |
| doi_str_mv | 10.1099/ijs.0.046581-0 |
| format | Article |
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Fernanda ; Veríssimo, António ; Morais, Paula V</creator><creatorcontrib>Chung, Ana Paula ; Tiago, Igor ; Nobre, M. Fernanda ; Veríssimo, António ; Morais, Paula V</creatorcontrib><description>A bacterial strain, A2-57T, recovered from a water sample collected in a uranium mine was taxonomically studied in detail. This strain was a Gram-reaction-negative, rod-shaped bacterium that grew optimally at 25 °C and at pH 6.0–7.0 and had a DNA G+C content of 55.0 mol%. Ubiquinone 8 (UQ-8) was the predominant respiratory quinone and the major fatty acids were C16 : 0, C17 : 0 cyclo, summed feature 3 (C16 : 1ω6c and/or ω7c and/or C15 : 0 iso 2-OH) and C18 : 1ω7c. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain A2-57T belonged to the family Oxalobacteraceae and formed a distinct branch with Glaciimonas immobilis Cr9-30T. Strain A2-57T shared approximately 97.3 % 16S rRNA sequence similarity with G. immobilis Cr9-30T and also showed high sequence similarity with members of the genera Herbaspirillum (96.3–97.0 %) and Collimonas (96.2–97.0 %). Although phylogenetically closely related to the type strain of G. immobilis, the low level of DNA–DNA hybridization between the two strains (21.6 %) and several physiological and biochemical properties indicated that the novel strain could be clearly distinguished from G. immobilis LMG 25547T. Therefore, it is concluded that strain A2-57T represents a novel species of the genus Glaciimonas, for which the name Glaciimonas singularis sp. nov. is proposed. 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Fernanda</creatorcontrib><creatorcontrib>Veríssimo, António</creatorcontrib><creatorcontrib>Morais, Paula V</creatorcontrib><title>Glaciimonas singularis sp. nov., isolated from a uranium mine wastewater treatment plant</title><title>International journal of systematic and evolutionary microbiology</title><addtitle>Int J Syst Evol Microbiol</addtitle><description>A bacterial strain, A2-57T, recovered from a water sample collected in a uranium mine was taxonomically studied in detail. This strain was a Gram-reaction-negative, rod-shaped bacterium that grew optimally at 25 °C and at pH 6.0–7.0 and had a DNA G+C content of 55.0 mol%. Ubiquinone 8 (UQ-8) was the predominant respiratory quinone and the major fatty acids were C16 : 0, C17 : 0 cyclo, summed feature 3 (C16 : 1ω6c and/or ω7c and/or C15 : 0 iso 2-OH) and C18 : 1ω7c. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain A2-57T belonged to the family Oxalobacteraceae and formed a distinct branch with Glaciimonas immobilis Cr9-30T. Strain A2-57T shared approximately 97.3 % 16S rRNA sequence similarity with G. immobilis Cr9-30T and also showed high sequence similarity with members of the genera Herbaspirillum (96.3–97.0 %) and Collimonas (96.2–97.0 %). Although phylogenetically closely related to the type strain of G. immobilis, the low level of DNA–DNA hybridization between the two strains (21.6 %) and several physiological and biochemical properties indicated that the novel strain could be clearly distinguished from G. immobilis LMG 25547T. Therefore, it is concluded that strain A2-57T represents a novel species of the genus Glaciimonas, for which the name Glaciimonas singularis sp. nov. is proposed. The type strain is A2-57T ( = CIP 110539T = LMG 27070T).</description><subject>bacteria</subject><subject>Bacterial Typing Techniques</subject><subject>Base Composition</subject><subject>DNA</subject><subject>DNA, Bacterial - genetics</subject><subject>fatty acids</subject><subject>Fatty Acids - analysis</subject><subject>Herbaspirillum</subject><subject>Mining</subject><subject>Molecular Sequence Data</subject><subject>Nucleic Acid Hybridization</subject><subject>nucleotide sequences</subject><subject>Oxalobacteraceae - classification</subject><subject>Oxalobacteraceae - genetics</subject><subject>Oxalobacteraceae - isolation & purification</subject><subject>Phylogeny</subject><subject>Portugal</subject><subject>ribosomal RNA</subject><subject>RNA, Ribosomal, 16S - genetics</subject><subject>Sequence Analysis, DNA</subject><subject>sequence homology</subject><subject>Ubiquinone - analysis</subject><subject>ubiquinones</subject><subject>Uranium</subject><subject>Waste Water - microbiology</subject><subject>wastewater treatment</subject><subject>Water Microbiology</subject><issn>1466-5034</issn><issn>1466-5026</issn><issn>1466-5034</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpNkD1PwzAQhi0EoqWwMoJHBhLOceIkI6qgIFVigEps1iV2Klf5wk6o-Pe4CiAmn3zPvbp7CLlkEDLI8zuzcyGEEIskYwEckTmLhQgS4PHxv3pGzpzbAfgPgFMyizhLszQSc_K-qrE0puladNSZdjvWaI0v-5C23Wd4S43rahy0opXtGop0tNiasaGNaTXdoxv03rctHazGodHtQPsa2-GcnFRYO33x8y7I5vHhbfkUrF9Wz8v7dVBylg-B1hGLSl6gKhTDKOVZKrDQWsWqYiLjmJQAeVHlqThsjyJOM6VVVMVZofxpfEFuptzedh-jdoNsjCt17XfQ3egk40Ikqc-NPBpOaGk756yuZG9Ng_ZLMpAHm9LblCAnmxL8wNVP9lg0Wv3hv_o8cD0BFXYSt16c3LxGwJKD6hgY8G8FZXp_</recordid><startdate>20130601</startdate><enddate>20130601</enddate><creator>Chung, Ana Paula</creator><creator>Tiago, Igor</creator><creator>Nobre, M. Fernanda</creator><creator>Veríssimo, António</creator><creator>Morais, Paula V</creator><general>International Union of Microbiological Societies</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20130601</creationdate><title>Glaciimonas singularis sp. nov., isolated from a uranium mine wastewater treatment plant</title><author>Chung, Ana Paula ; Tiago, Igor ; Nobre, M. Fernanda ; Veríssimo, António ; Morais, Paula V</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c319t-ee212c3badbd1a273876abeed4df1683a5c009bf9760146a6478ded2f48bd5033</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>bacteria</topic><topic>Bacterial Typing Techniques</topic><topic>Base Composition</topic><topic>DNA</topic><topic>DNA, Bacterial - genetics</topic><topic>fatty acids</topic><topic>Fatty Acids - analysis</topic><topic>Herbaspirillum</topic><topic>Mining</topic><topic>Molecular Sequence Data</topic><topic>Nucleic Acid Hybridization</topic><topic>nucleotide sequences</topic><topic>Oxalobacteraceae - classification</topic><topic>Oxalobacteraceae - genetics</topic><topic>Oxalobacteraceae - isolation & purification</topic><topic>Phylogeny</topic><topic>Portugal</topic><topic>ribosomal RNA</topic><topic>RNA, Ribosomal, 16S - genetics</topic><topic>Sequence Analysis, DNA</topic><topic>sequence homology</topic><topic>Ubiquinone - analysis</topic><topic>ubiquinones</topic><topic>Uranium</topic><topic>Waste Water - microbiology</topic><topic>wastewater treatment</topic><topic>Water Microbiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chung, Ana Paula</creatorcontrib><creatorcontrib>Tiago, Igor</creatorcontrib><creatorcontrib>Nobre, M. Fernanda</creatorcontrib><creatorcontrib>Veríssimo, António</creatorcontrib><creatorcontrib>Morais, Paula V</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>International journal of systematic and evolutionary microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chung, Ana Paula</au><au>Tiago, Igor</au><au>Nobre, M. Fernanda</au><au>Veríssimo, António</au><au>Morais, Paula V</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Glaciimonas singularis sp. nov., isolated from a uranium mine wastewater treatment plant</atitle><jtitle>International journal of systematic and evolutionary microbiology</jtitle><addtitle>Int J Syst Evol Microbiol</addtitle><date>2013-06-01</date><risdate>2013</risdate><volume>63</volume><issue>Pt 6</issue><spage>2344</spage><epage>2350</epage><pages>2344-2350</pages><issn>1466-5034</issn><issn>1466-5026</issn><eissn>1466-5034</eissn><abstract>A bacterial strain, A2-57T, recovered from a water sample collected in a uranium mine was taxonomically studied in detail. This strain was a Gram-reaction-negative, rod-shaped bacterium that grew optimally at 25 °C and at pH 6.0–7.0 and had a DNA G+C content of 55.0 mol%. Ubiquinone 8 (UQ-8) was the predominant respiratory quinone and the major fatty acids were C16 : 0, C17 : 0 cyclo, summed feature 3 (C16 : 1ω6c and/or ω7c and/or C15 : 0 iso 2-OH) and C18 : 1ω7c. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain A2-57T belonged to the family Oxalobacteraceae and formed a distinct branch with Glaciimonas immobilis Cr9-30T. Strain A2-57T shared approximately 97.3 % 16S rRNA sequence similarity with G. immobilis Cr9-30T and also showed high sequence similarity with members of the genera Herbaspirillum (96.3–97.0 %) and Collimonas (96.2–97.0 %). Although phylogenetically closely related to the type strain of G. immobilis, the low level of DNA–DNA hybridization between the two strains (21.6 %) and several physiological and biochemical properties indicated that the novel strain could be clearly distinguished from G. immobilis LMG 25547T. Therefore, it is concluded that strain A2-57T represents a novel species of the genus Glaciimonas, for which the name Glaciimonas singularis sp. nov. is proposed. The type strain is A2-57T ( = CIP 110539T = LMG 27070T).</abstract><cop>England</cop><pub>International Union of Microbiological Societies</pub><pmid>23178726</pmid><doi>10.1099/ijs.0.046581-0</doi><tpages>7</tpages></addata></record> |
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| subjects | bacteria Bacterial Typing Techniques Base Composition DNA DNA, Bacterial - genetics fatty acids Fatty Acids - analysis Herbaspirillum Mining Molecular Sequence Data Nucleic Acid Hybridization nucleotide sequences Oxalobacteraceae - classification Oxalobacteraceae - genetics Oxalobacteraceae - isolation & purification Phylogeny Portugal ribosomal RNA RNA, Ribosomal, 16S - genetics Sequence Analysis, DNA sequence homology Ubiquinone - analysis ubiquinones Uranium Waste Water - microbiology wastewater treatment Water Microbiology |
| title | Glaciimonas singularis sp. nov., isolated from a uranium mine wastewater treatment plant |
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