A data treatment method for detecting fluorescence anisotropy peaks in capillary electropherograms
[Display omitted] ► We explain the absence of fluorescence anisotropy peaks for capillary separations. ► We developed a method to visualize fluorescence anisotropy peaks for separations. ► This method was applied to a separation of amyloid beta peptide aggregates. A data treatment method is presente...
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| Veröffentlicht in: | Analytica chimica acta 2012-08, Vol.739, p.99-103 |
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| container_title | Analytica chimica acta |
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| creator | Picou, Ryan A. Kheterpal, Indu Gilman, S. Douglass |
| description | [Display omitted]
► We explain the absence of fluorescence anisotropy peaks for capillary separations. ► We developed a method to visualize fluorescence anisotropy peaks for separations. ► This method was applied to a separation of amyloid beta peptide aggregates.
A data treatment method is presented to detect fluorescence anisotropy (FA) peaks in capillary electrophoresis electropherograms. The data treatment method converts plots of fluorescence anisotropy vs. time that contain no peaks that are distinguishable from the noise of the anisotropy background into plots that show distinct fluorescence anisotropy peaks. The method was demonstrated using laser-induced fluorescence anisotropy data from individual Aβ (1–42) aggregates separated using capillary electrophoresis. Applying this data treatment method enabled the detection of anisotropy peaks for individual Aβ aggregate fluorescence peaks that were not observed prior to the data treatment method. The data treatment method is not specifically designed for Aβ aggregate analysis or capillary electrophoresis, and it should be applicable to other applications and other separation methods with FA detection. |
| doi_str_mv | 10.1016/j.aca.2012.06.023 |
| format | Article |
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► We explain the absence of fluorescence anisotropy peaks for capillary separations. ► We developed a method to visualize fluorescence anisotropy peaks for separations. ► This method was applied to a separation of amyloid beta peptide aggregates.
A data treatment method is presented to detect fluorescence anisotropy (FA) peaks in capillary electrophoresis electropherograms. The data treatment method converts plots of fluorescence anisotropy vs. time that contain no peaks that are distinguishable from the noise of the anisotropy background into plots that show distinct fluorescence anisotropy peaks. The method was demonstrated using laser-induced fluorescence anisotropy data from individual Aβ (1–42) aggregates separated using capillary electrophoresis. Applying this data treatment method enabled the detection of anisotropy peaks for individual Aβ aggregate fluorescence peaks that were not observed prior to the data treatment method. The data treatment method is not specifically designed for Aβ aggregate analysis or capillary electrophoresis, and it should be applicable to other applications and other separation methods with FA detection.</description><identifier>ISSN: 0003-2670</identifier><identifier>EISSN: 1873-4324</identifier><identifier>DOI: 10.1016/j.aca.2012.06.023</identifier><identifier>PMID: 22819055</identifier><identifier>CODEN: ACACAM</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Aggregates ; Algorithms ; Amyloid beta peptide ; Amyloid beta-Peptides - analysis ; Analytical chemistry ; Anisotropy ; Background noise ; Capillarity ; Capillary electrophoresis ; Chemistry ; Chromatographic methods and physical methods associated with chromatography ; Data treatment ; Electrophoresis ; Electrophoresis, Capillary ; Exact sciences and technology ; Fluorescence ; Fluorescence anisotropy ; Fluorescence Polarization ; Humans ; Lasers ; Mathematical analysis ; Noise ; Other chromatographic methods ; Peptide Fragments - analysis ; Signal Processing, Computer-Assisted ; Spectrometric and optical methods</subject><ispartof>Analytica chimica acta, 2012-08, Vol.739, p.99-103</ispartof><rights>2012 Elsevier B.V.</rights><rights>2015 INIST-CNRS</rights><rights>Copyright © 2012 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c416t-67ab7c20022348cf8d8ff4509ea868f8a93a41f077c662c3a3b8ac8737d6ea6c3</citedby><cites>FETCH-LOGICAL-c416t-67ab7c20022348cf8d8ff4509ea868f8a93a41f077c662c3a3b8ac8737d6ea6c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.aca.2012.06.023$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=26175884$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22819055$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Picou, Ryan A.</creatorcontrib><creatorcontrib>Kheterpal, Indu</creatorcontrib><creatorcontrib>Gilman, S. Douglass</creatorcontrib><title>A data treatment method for detecting fluorescence anisotropy peaks in capillary electropherograms</title><title>Analytica chimica acta</title><addtitle>Anal Chim Acta</addtitle><description>[Display omitted]
► We explain the absence of fluorescence anisotropy peaks for capillary separations. ► We developed a method to visualize fluorescence anisotropy peaks for separations. ► This method was applied to a separation of amyloid beta peptide aggregates.
A data treatment method is presented to detect fluorescence anisotropy (FA) peaks in capillary electrophoresis electropherograms. The data treatment method converts plots of fluorescence anisotropy vs. time that contain no peaks that are distinguishable from the noise of the anisotropy background into plots that show distinct fluorescence anisotropy peaks. The method was demonstrated using laser-induced fluorescence anisotropy data from individual Aβ (1–42) aggregates separated using capillary electrophoresis. Applying this data treatment method enabled the detection of anisotropy peaks for individual Aβ aggregate fluorescence peaks that were not observed prior to the data treatment method. The data treatment method is not specifically designed for Aβ aggregate analysis or capillary electrophoresis, and it should be applicable to other applications and other separation methods with FA detection.</description><subject>Aggregates</subject><subject>Algorithms</subject><subject>Amyloid beta peptide</subject><subject>Amyloid beta-Peptides - analysis</subject><subject>Analytical chemistry</subject><subject>Anisotropy</subject><subject>Background noise</subject><subject>Capillarity</subject><subject>Capillary electrophoresis</subject><subject>Chemistry</subject><subject>Chromatographic methods and physical methods associated with chromatography</subject><subject>Data treatment</subject><subject>Electrophoresis</subject><subject>Electrophoresis, Capillary</subject><subject>Exact sciences and technology</subject><subject>Fluorescence</subject><subject>Fluorescence anisotropy</subject><subject>Fluorescence Polarization</subject><subject>Humans</subject><subject>Lasers</subject><subject>Mathematical analysis</subject><subject>Noise</subject><subject>Other chromatographic methods</subject><subject>Peptide Fragments - analysis</subject><subject>Signal Processing, Computer-Assisted</subject><subject>Spectrometric and optical methods</subject><issn>0003-2670</issn><issn>1873-4324</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUtvFDEQhC0URJYkP4AL8iUSlxn8mLG9yila8ZIicYGz1etpJ15mxhPbGyn_Hi-7wA1OVstftaqrCHnDWcsZV-93LThoBeOiZaplQr4gK260bDopujOyYozJRijNzsnrnHd1FJx1r8i5EIavWd-vyPaWDlCAloRQJpwLnbA8xIH6mOiABV0J8z314z4mzA5nhxTmkGNJcXmmC8KPTMNMHSxhHCE9Uxyrpn4-YIr3CaZ8SV56GDNend4L8v3jh2-bz83d109fNrd3jeu4Ko3SsNVOVI9CdsZ5Mxjvu56tEYwy3sBaQsc909opJZwEuTXg6rV6UAjKyQvy7rh3SfFxj7nYKVTH1dWMcZ8tl6rnXCnF_o8yoZXhXMiK8iPqUsw5obdLClM9tEL20ILd2dqCPbRgmbLsl-btaf1-O-HwR_E79gpcnwDIDkafYHYh_-UU170xXeVujhzW3J4CJptdOHQwhFRTtkMM_7DxEzVEpTs</recordid><startdate>20120820</startdate><enddate>20120820</enddate><creator>Picou, Ryan A.</creator><creator>Kheterpal, Indu</creator><creator>Gilman, S. 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Douglass</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c416t-67ab7c20022348cf8d8ff4509ea868f8a93a41f077c662c3a3b8ac8737d6ea6c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Aggregates</topic><topic>Algorithms</topic><topic>Amyloid beta peptide</topic><topic>Amyloid beta-Peptides - analysis</topic><topic>Analytical chemistry</topic><topic>Anisotropy</topic><topic>Background noise</topic><topic>Capillarity</topic><topic>Capillary electrophoresis</topic><topic>Chemistry</topic><topic>Chromatographic methods and physical methods associated with chromatography</topic><topic>Data treatment</topic><topic>Electrophoresis</topic><topic>Electrophoresis, Capillary</topic><topic>Exact sciences and technology</topic><topic>Fluorescence</topic><topic>Fluorescence anisotropy</topic><topic>Fluorescence Polarization</topic><topic>Humans</topic><topic>Lasers</topic><topic>Mathematical analysis</topic><topic>Noise</topic><topic>Other chromatographic methods</topic><topic>Peptide Fragments - analysis</topic><topic>Signal Processing, Computer-Assisted</topic><topic>Spectrometric and optical methods</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Picou, Ryan A.</creatorcontrib><creatorcontrib>Kheterpal, Indu</creatorcontrib><creatorcontrib>Gilman, S. 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Douglass</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A data treatment method for detecting fluorescence anisotropy peaks in capillary electropherograms</atitle><jtitle>Analytica chimica acta</jtitle><addtitle>Anal Chim Acta</addtitle><date>2012-08-20</date><risdate>2012</risdate><volume>739</volume><spage>99</spage><epage>103</epage><pages>99-103</pages><issn>0003-2670</issn><eissn>1873-4324</eissn><coden>ACACAM</coden><abstract>[Display omitted]
► We explain the absence of fluorescence anisotropy peaks for capillary separations. ► We developed a method to visualize fluorescence anisotropy peaks for separations. ► This method was applied to a separation of amyloid beta peptide aggregates.
A data treatment method is presented to detect fluorescence anisotropy (FA) peaks in capillary electrophoresis electropherograms. The data treatment method converts plots of fluorescence anisotropy vs. time that contain no peaks that are distinguishable from the noise of the anisotropy background into plots that show distinct fluorescence anisotropy peaks. The method was demonstrated using laser-induced fluorescence anisotropy data from individual Aβ (1–42) aggregates separated using capillary electrophoresis. Applying this data treatment method enabled the detection of anisotropy peaks for individual Aβ aggregate fluorescence peaks that were not observed prior to the data treatment method. The data treatment method is not specifically designed for Aβ aggregate analysis or capillary electrophoresis, and it should be applicable to other applications and other separation methods with FA detection.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>22819055</pmid><doi>10.1016/j.aca.2012.06.023</doi><tpages>5</tpages></addata></record> |
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| subjects | Aggregates Algorithms Amyloid beta peptide Amyloid beta-Peptides - analysis Analytical chemistry Anisotropy Background noise Capillarity Capillary electrophoresis Chemistry Chromatographic methods and physical methods associated with chromatography Data treatment Electrophoresis Electrophoresis, Capillary Exact sciences and technology Fluorescence Fluorescence anisotropy Fluorescence Polarization Humans Lasers Mathematical analysis Noise Other chromatographic methods Peptide Fragments - analysis Signal Processing, Computer-Assisted Spectrometric and optical methods |
| title | A data treatment method for detecting fluorescence anisotropy peaks in capillary electropherograms |
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