Determination of Enterotoxigenic and Methicillin Resistant Staphylococcus aureus in Ice Cream
The aim of this study was to determine the prevalence of enterotoxigenic and methicillin‐resistant Staphylococcus aureus in ice creams. After culture‐based identification of isolates, the presence of 16S rRNA and nuc was confirmed by mPCR. S. aureus was identified in 18 of 56 fruity (32.1%), 4 of 32...
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| Veröffentlicht in: | Journal of food science 2013-05, Vol.78 (5), p.M738-M741 |
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| creator | Gücükoğlu, Ali Çadirci, Özgür Terzi, Göknur Kevenk, T. Onur Alişarli, Mustafa |
| description | The aim of this study was to determine the prevalence of enterotoxigenic and methicillin‐resistant Staphylococcus aureus in ice creams. After culture‐based identification of isolates, the presence of 16S rRNA and nuc was confirmed by mPCR. S. aureus was identified in 18 of 56 fruity (32.1%), 4 of 32 vanilla (12.5%), and 1 of 12 chocolate (8.3%) ice creams. S. aureus was identified as 38 isolates in 23 ice cream samples by culture‐based techniques, but only 35 isolates were confirmed by PCR as S. aureus. To determine the enterotoxigenic properties of PCR‐confirmed S. aureus isolates, a toxin detection kit was used (SET RPLA®). Of the 12 enterotoxigenic S. aureus isolates, 9 SEB (75%), 1 SED (8.3%), 1 SEB+SED (8.3%), and 1 SEA+SEB+SED (8.3%) expressing isolates were found. The presence of enterotoxin genes (sea, seb, sed) was identified in 13 (37.1%) out of 35 isolates by the mPCR technique. In the ice cream isolates, the sea, seb, and sed genes were detected: 1 sea (7.6%), 9 seb (69.2%), 1 sed (7.6%), 1 seb+sed (7.6%), and 1 sea+seb+sed (7.6%), respectively. The sec gene was not detected in any of these isolates. One of the 35 (2.8%) S. aureus strain was mecA positive. |
| doi_str_mv | 10.1111/1750-3841.12093 |
| format | Article |
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Onur ; Alişarli, Mustafa</creator><creatorcontrib>Gücükoğlu, Ali ; Çadirci, Özgür ; Terzi, Göknur ; Kevenk, T. Onur ; Alişarli, Mustafa</creatorcontrib><description>The aim of this study was to determine the prevalence of enterotoxigenic and methicillin‐resistant Staphylococcus aureus in ice creams. After culture‐based identification of isolates, the presence of 16S rRNA and nuc was confirmed by mPCR. S. aureus was identified in 18 of 56 fruity (32.1%), 4 of 32 vanilla (12.5%), and 1 of 12 chocolate (8.3%) ice creams. S. aureus was identified as 38 isolates in 23 ice cream samples by culture‐based techniques, but only 35 isolates were confirmed by PCR as S. aureus. To determine the enterotoxigenic properties of PCR‐confirmed S. aureus isolates, a toxin detection kit was used (SET RPLA®). Of the 12 enterotoxigenic S. aureus isolates, 9 SEB (75%), 1 SED (8.3%), 1 SEB+SED (8.3%), and 1 SEA+SEB+SED (8.3%) expressing isolates were found. The presence of enterotoxin genes (sea, seb, sed) was identified in 13 (37.1%) out of 35 isolates by the mPCR technique. In the ice cream isolates, the sea, seb, and sed genes were detected: 1 sea (7.6%), 9 seb (69.2%), 1 sed (7.6%), 1 seb+sed (7.6%), and 1 sea+seb+sed (7.6%), respectively. The sec gene was not detected in any of these isolates. One of the 35 (2.8%) S. aureus strain was mecA positive.</description><identifier>ISSN: 0022-1147</identifier><identifier>EISSN: 1750-3841</identifier><identifier>DOI: 10.1111/1750-3841.12093</identifier><identifier>PMID: 23527618</identifier><identifier>CODEN: JFDSAZ</identifier><language>eng</language><publisher>Hoboken, NJ: Blackwell Publishing Ltd</publisher><subject>Biological and medical sciences ; DNA, Bacterial - genetics ; Drug resistance ; Enterotoxigenic gene ; Enterotoxins - isolation & purification ; Food Contamination - analysis ; Food industries ; Food Microbiology ; Fundamental and applied biological sciences. Psychology ; Genes ; Ice cream ; Ice Cream - microbiology ; Methicillin-Resistant Staphylococcus aureus - classification ; Methicillin-Resistant Staphylococcus aureus - genetics ; Methicillin-Resistant Staphylococcus aureus - isolation & purification ; Milk and cheese industries. Ice creams ; MRSA ; PCR ; Polymerase chain reaction ; Polymerase Chain Reaction - methods ; RNA, Ribosomal, 16S - genetics ; S. aureus ; Staphylococcus aureus ; Staphylococcus aureus - classification ; Staphylococcus aureus - genetics ; Staphylococcus aureus - isolation & purification ; Staphylococcus infections ; Vanilla</subject><ispartof>Journal of food science, 2013-05, Vol.78 (5), p.M738-M741</ispartof><rights>2013 Institute of Food Technologists</rights><rights>2014 INIST-CNRS</rights><rights>Copyright Institute of Food Technologists May 2013</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5143-7f1004c0ff49e612069be4235402f08077b73c5033ac184c6708490755ebfe223</citedby><cites>FETCH-LOGICAL-c5143-7f1004c0ff49e612069be4235402f08077b73c5033ac184c6708490755ebfe223</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2F1750-3841.12093$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2F1750-3841.12093$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1416,27922,27923,45572,45573</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=27516841$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23527618$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Gücükoğlu, Ali</creatorcontrib><creatorcontrib>Çadirci, Özgür</creatorcontrib><creatorcontrib>Terzi, Göknur</creatorcontrib><creatorcontrib>Kevenk, T. Onur</creatorcontrib><creatorcontrib>Alişarli, Mustafa</creatorcontrib><title>Determination of Enterotoxigenic and Methicillin Resistant Staphylococcus aureus in Ice Cream</title><title>Journal of food science</title><addtitle>Journal of Food Science</addtitle><description>The aim of this study was to determine the prevalence of enterotoxigenic and methicillin‐resistant Staphylococcus aureus in ice creams. After culture‐based identification of isolates, the presence of 16S rRNA and nuc was confirmed by mPCR. S. aureus was identified in 18 of 56 fruity (32.1%), 4 of 32 vanilla (12.5%), and 1 of 12 chocolate (8.3%) ice creams. S. aureus was identified as 38 isolates in 23 ice cream samples by culture‐based techniques, but only 35 isolates were confirmed by PCR as S. aureus. To determine the enterotoxigenic properties of PCR‐confirmed S. aureus isolates, a toxin detection kit was used (SET RPLA®). Of the 12 enterotoxigenic S. aureus isolates, 9 SEB (75%), 1 SED (8.3%), 1 SEB+SED (8.3%), and 1 SEA+SEB+SED (8.3%) expressing isolates were found. The presence of enterotoxin genes (sea, seb, sed) was identified in 13 (37.1%) out of 35 isolates by the mPCR technique. In the ice cream isolates, the sea, seb, and sed genes were detected: 1 sea (7.6%), 9 seb (69.2%), 1 sed (7.6%), 1 seb+sed (7.6%), and 1 sea+seb+sed (7.6%), respectively. The sec gene was not detected in any of these isolates. One of the 35 (2.8%) S. aureus strain was mecA positive.</description><subject>Biological and medical sciences</subject><subject>DNA, Bacterial - genetics</subject><subject>Drug resistance</subject><subject>Enterotoxigenic gene</subject><subject>Enterotoxins - isolation & purification</subject><subject>Food Contamination - analysis</subject><subject>Food industries</subject><subject>Food Microbiology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genes</subject><subject>Ice cream</subject><subject>Ice Cream - microbiology</subject><subject>Methicillin-Resistant Staphylococcus aureus - classification</subject><subject>Methicillin-Resistant Staphylococcus aureus - genetics</subject><subject>Methicillin-Resistant Staphylococcus aureus - isolation & purification</subject><subject>Milk and cheese industries. Ice creams</subject><subject>MRSA</subject><subject>PCR</subject><subject>Polymerase chain reaction</subject><subject>Polymerase Chain Reaction - methods</subject><subject>RNA, Ribosomal, 16S - genetics</subject><subject>S. aureus</subject><subject>Staphylococcus aureus</subject><subject>Staphylococcus aureus - classification</subject><subject>Staphylococcus aureus - genetics</subject><subject>Staphylococcus aureus - isolation & purification</subject><subject>Staphylococcus infections</subject><subject>Vanilla</subject><issn>0022-1147</issn><issn>1750-3841</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkctv1DAQxi0EotvCmRuKhJC4pB3Hr-SItg9atSAoDwkJWV53Ql0Se7ETtfvf47DbReICvow8-s038-kj5BmFfZrfAVUCSlZzuk8raNgDMtt2HpIZQFWVlHK1Q3ZTuoHpz-RjslMxUSlJ6xn5dogDxt55M7jgi9AWRz43whDu3Hf0zhbGXxUXOFw767rO-eIDJpcG44ficjDL61UXbLB2TIUZI-aSkVOLxTyi6Z-QR63pEj7d1D3y6fjo4_xNef7u5HT--ry0gnJWqpYCcAttyxuU2YlsFsjzkRyqFmpQaqGYFcCYsbTmViqoeQNKCFy0mD3tkVdr3WUMP0dMg-5dsth1xmMYk6ZMCpCyBvgPlDccQLJJ9cVf6E0Yo89GMpX1Kl4LkamDNWVjSCliq5fR9SauNAU9haSnSPQUif4dUp54vtEdFz1ebfn7VDLwcgOYZE3XRuOtS384JajMcpmTa-7Wdbj61159dnx4eX9BuR7MSeLddtDEH1oqpoT-8vZEv_-qqovPFegz9gsYkrUc</recordid><startdate>201305</startdate><enddate>201305</enddate><creator>Gücükoğlu, Ali</creator><creator>Çadirci, Özgür</creator><creator>Terzi, Göknur</creator><creator>Kevenk, T. Onur</creator><creator>Alişarli, Mustafa</creator><general>Blackwell Publishing Ltd</general><general>Wiley</general><general>Wiley Subscription Services, Inc</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7QR</scope><scope>7ST</scope><scope>7T7</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>F28</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>SOI</scope><scope>7X8</scope><scope>7QL</scope></search><sort><creationdate>201305</creationdate><title>Determination of Enterotoxigenic and Methicillin Resistant Staphylococcus aureus in Ice Cream</title><author>Gücükoğlu, Ali ; Çadirci, Özgür ; Terzi, Göknur ; Kevenk, T. Onur ; Alişarli, Mustafa</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5143-7f1004c0ff49e612069be4235402f08077b73c5033ac184c6708490755ebfe223</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Biological and medical sciences</topic><topic>DNA, Bacterial - genetics</topic><topic>Drug resistance</topic><topic>Enterotoxigenic gene</topic><topic>Enterotoxins - isolation & purification</topic><topic>Food Contamination - analysis</topic><topic>Food industries</topic><topic>Food Microbiology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genes</topic><topic>Ice cream</topic><topic>Ice Cream - microbiology</topic><topic>Methicillin-Resistant Staphylococcus aureus - classification</topic><topic>Methicillin-Resistant Staphylococcus aureus - genetics</topic><topic>Methicillin-Resistant Staphylococcus aureus - isolation & purification</topic><topic>Milk and cheese industries. Ice creams</topic><topic>MRSA</topic><topic>PCR</topic><topic>Polymerase chain reaction</topic><topic>Polymerase Chain Reaction - methods</topic><topic>RNA, Ribosomal, 16S - genetics</topic><topic>S. aureus</topic><topic>Staphylococcus aureus</topic><topic>Staphylococcus aureus - classification</topic><topic>Staphylococcus aureus - genetics</topic><topic>Staphylococcus aureus - isolation & purification</topic><topic>Staphylococcus infections</topic><topic>Vanilla</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gücükoğlu, Ali</creatorcontrib><creatorcontrib>Çadirci, Özgür</creatorcontrib><creatorcontrib>Terzi, Göknur</creatorcontrib><creatorcontrib>Kevenk, T. 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Onur</au><au>Alişarli, Mustafa</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Determination of Enterotoxigenic and Methicillin Resistant Staphylococcus aureus in Ice Cream</atitle><jtitle>Journal of food science</jtitle><addtitle>Journal of Food Science</addtitle><date>2013-05</date><risdate>2013</risdate><volume>78</volume><issue>5</issue><spage>M738</spage><epage>M741</epage><pages>M738-M741</pages><issn>0022-1147</issn><eissn>1750-3841</eissn><coden>JFDSAZ</coden><abstract>The aim of this study was to determine the prevalence of enterotoxigenic and methicillin‐resistant Staphylococcus aureus in ice creams. After culture‐based identification of isolates, the presence of 16S rRNA and nuc was confirmed by mPCR. S. aureus was identified in 18 of 56 fruity (32.1%), 4 of 32 vanilla (12.5%), and 1 of 12 chocolate (8.3%) ice creams. S. aureus was identified as 38 isolates in 23 ice cream samples by culture‐based techniques, but only 35 isolates were confirmed by PCR as S. aureus. To determine the enterotoxigenic properties of PCR‐confirmed S. aureus isolates, a toxin detection kit was used (SET RPLA®). Of the 12 enterotoxigenic S. aureus isolates, 9 SEB (75%), 1 SED (8.3%), 1 SEB+SED (8.3%), and 1 SEA+SEB+SED (8.3%) expressing isolates were found. The presence of enterotoxin genes (sea, seb, sed) was identified in 13 (37.1%) out of 35 isolates by the mPCR technique. In the ice cream isolates, the sea, seb, and sed genes were detected: 1 sea (7.6%), 9 seb (69.2%), 1 sed (7.6%), 1 seb+sed (7.6%), and 1 sea+seb+sed (7.6%), respectively. The sec gene was not detected in any of these isolates. One of the 35 (2.8%) S. aureus strain was mecA positive.</abstract><cop>Hoboken, NJ</cop><pub>Blackwell Publishing Ltd</pub><pmid>23527618</pmid><doi>10.1111/1750-3841.12093</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Biological and medical sciences DNA, Bacterial - genetics Drug resistance Enterotoxigenic gene Enterotoxins - isolation & purification Food Contamination - analysis Food industries Food Microbiology Fundamental and applied biological sciences. Psychology Genes Ice cream Ice Cream - microbiology Methicillin-Resistant Staphylococcus aureus - classification Methicillin-Resistant Staphylococcus aureus - genetics Methicillin-Resistant Staphylococcus aureus - isolation & purification Milk and cheese industries. Ice creams MRSA PCR Polymerase chain reaction Polymerase Chain Reaction - methods RNA, Ribosomal, 16S - genetics S. aureus Staphylococcus aureus Staphylococcus aureus - classification Staphylococcus aureus - genetics Staphylococcus aureus - isolation & purification Staphylococcus infections Vanilla |
| title | Determination of Enterotoxigenic and Methicillin Resistant Staphylococcus aureus in Ice Cream |
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