Brownian nanoimaging of interface dynamics and ligand–receptor binding at cell surfaces in 3-D

We describe a method for nanoimaging interfacial dynamics and ligand–receptor binding at surfaces of live cells in 3-D. The imaging probe is a 1-μm diameter glass bead confined by a soft laser trap to create a “cloud” of fluctuating states. Using a facile on-line method of video image analysis, the...

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Veröffentlicht in:	Methods (San Diego, Calif.) Calif.), 2013-04, Vol.60 (2), p.214-224
Hauptverfasser:	Kuznetsov, Igor R., Evans, Evan A.
Format:	Artikel
Sprache:	eng
Schlagworte:	Algorithms Brownian particle imaging Calibration Cell Surface Extensions - metabolism Cell Surface Extensions - ultrastructure Fast telemetry of receptor binding Fibronectins - metabolism Humans Integrin alpha4beta1 - metabolism Jurkat Cells Kinetics Ligands Ligand–receptor binding kinetics Likelihood Functions Markov Chains Microscopy, Video - methods Models, Biological Nanoscale tracking in 3D Nanotechnology - methods Off-line super resolution Optical Tweezers Protein Binding Surface Properties Surface steric topography Thermodynamics
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