The effect of electroacupuncture on osteotomy gap healing in a rat fibula model
Objective To examine the effect of electroacupuncture (EA) on osteotomy gap healing in a rat fibula model. Methods A total of 40 12-week-old male Wistar rats underwent unilateral open osteotomy of the fibula to create a 2 mm gap. The rats were randomly assigned to an EA group (n=20) and a control gr...
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| Veröffentlicht in: | Acupuncture in medicine : journal of the British Medical Acupuncture Society 2013-06, Vol.31 (2), p.222-227 |
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| container_title | Acupuncture in medicine : journal of the British Medical Acupuncture Society |
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| creator | Inoue, Motohiro Nakajima, Miwa Hojo, Tatsuya Itoi, Megumi Kitakoji, Hiroshi |
| description | Objective To examine the effect of electroacupuncture (EA) on osteotomy gap healing in a rat fibula model. Methods A total of 40 12-week-old male Wistar rats underwent unilateral open osteotomy of the fibula to create a 2 mm gap. The rats were randomly assigned to an EA group (n=20) and a control group (n=20). In the EA group, a cathodal electrode was connected to an acupuncture needle percutaneously at the surgery site, while another acupuncture needle inserted 15 mm proximal to the surgery site was used as an anodal electrode. EA was performed 5 days a week for 6 weeks. The control group received no treatment. Some rats were killed at 3 days and 1 week after surgery and the cut end of the distal part of the fibula was surgically removed and histologically assessed. Haematoxylin and eosin (H&E) staining was used to measure total cell count and immunohistochemical staining to assess the increase in the bone morphogenetic protein 2 (BMP-2)-positive cells. The healing process was also assessed weekly after surgery via x-ray examinations. Results At each time point, total cell count showed a marked increase in the EA group (p |
| doi_str_mv | 10.1136/acupmed-2012-010294 |
| format | Article |
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Methods A total of 40 12-week-old male Wistar rats underwent unilateral open osteotomy of the fibula to create a 2 mm gap. The rats were randomly assigned to an EA group (n=20) and a control group (n=20). In the EA group, a cathodal electrode was connected to an acupuncture needle percutaneously at the surgery site, while another acupuncture needle inserted 15 mm proximal to the surgery site was used as an anodal electrode. EA was performed 5 days a week for 6 weeks. The control group received no treatment. Some rats were killed at 3 days and 1 week after surgery and the cut end of the distal part of the fibula was surgically removed and histologically assessed. Haematoxylin and eosin (H&E) staining was used to measure total cell count and immunohistochemical staining to assess the increase in the bone morphogenetic protein 2 (BMP-2)-positive cells. The healing process was also assessed weekly after surgery via x-ray examinations. Results At each time point, total cell count showed a marked increase in the EA group (p<0.05), while BMP-2 expression showed a tendency to increase in the EA group. Radiological examination showed a marked reduction in the distance between the cut ends of the fibula in the EA group. Conclusions There was a marked increase in cell count and expression of growth factor in the EA group. These results indicate direct current EA could be useful for promoting bone healing.</description><identifier>ISSN: 0964-5284</identifier><identifier>EISSN: 1759-9873</identifier><identifier>DOI: 10.1136/acupmed-2012-010294</identifier><identifier>PMID: 23542071</identifier><language>eng</language><publisher>England: BMJ Publishing Group Ltd</publisher><subject>Animals ; Disease Models, Animal ; Electroacupuncture - methods ; Fibula - injuries ; Fibula - pathology ; Fracture Healing - physiology ; Fractures, Bone - therapy ; Osteotomy ; Rats ; Rats, Wistar</subject><ispartof>Acupuncture in medicine : journal of the British Medical Acupuncture Society, 2013-06, Vol.31 (2), p.222-227</ispartof><rights>Published by the BMJ Publishing Group Limited. For permission to use (where not already granted under a licence) please go to http://group.bmj.com/group/rights-licensing/permissions</rights><rights>Copyright British Medical Acupuncture Society Jun 2013</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-b476t-5e5c222c0553ba54db8a43736a389de8f68e99d129117bfa6841e1a83a6c56a53</citedby><cites>FETCH-LOGICAL-b476t-5e5c222c0553ba54db8a43736a389de8f68e99d129117bfa6841e1a83a6c56a53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781,27905,27906</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23542071$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Inoue, Motohiro</creatorcontrib><creatorcontrib>Nakajima, Miwa</creatorcontrib><creatorcontrib>Hojo, Tatsuya</creatorcontrib><creatorcontrib>Itoi, Megumi</creatorcontrib><creatorcontrib>Kitakoji, Hiroshi</creatorcontrib><title>The effect of electroacupuncture on osteotomy gap healing in a rat fibula model</title><title>Acupuncture in medicine : journal of the British Medical Acupuncture Society</title><addtitle>Acupunct Med</addtitle><description>Objective To examine the effect of electroacupuncture (EA) on osteotomy gap healing in a rat fibula model. Methods A total of 40 12-week-old male Wistar rats underwent unilateral open osteotomy of the fibula to create a 2 mm gap. The rats were randomly assigned to an EA group (n=20) and a control group (n=20). In the EA group, a cathodal electrode was connected to an acupuncture needle percutaneously at the surgery site, while another acupuncture needle inserted 15 mm proximal to the surgery site was used as an anodal electrode. EA was performed 5 days a week for 6 weeks. The control group received no treatment. Some rats were killed at 3 days and 1 week after surgery and the cut end of the distal part of the fibula was surgically removed and histologically assessed. Haematoxylin and eosin (H&E) staining was used to measure total cell count and immunohistochemical staining to assess the increase in the bone morphogenetic protein 2 (BMP-2)-positive cells. The healing process was also assessed weekly after surgery via x-ray examinations. Results At each time point, total cell count showed a marked increase in the EA group (p<0.05), while BMP-2 expression showed a tendency to increase in the EA group. Radiological examination showed a marked reduction in the distance between the cut ends of the fibula in the EA group. Conclusions There was a marked increase in cell count and expression of growth factor in the EA group. These results indicate direct current EA could be useful for promoting bone healing.</description><subject>Animals</subject><subject>Disease Models, Animal</subject><subject>Electroacupuncture - methods</subject><subject>Fibula - injuries</subject><subject>Fibula - pathology</subject><subject>Fracture Healing - physiology</subject><subject>Fractures, Bone - therapy</subject><subject>Osteotomy</subject><subject>Rats</subject><subject>Rats, Wistar</subject><issn>0964-5284</issn><issn>1759-9873</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><recordid>eNqNkE1rFEEQhhtRzCb6CwRp8OJlYn9_HHXRqIQEJOqxqZmpSWadmd50z4D59_YyGw-ePFVBPe9L8RDyirNzzqV5B82yH7GtBOOiYpwJr56QDbfaV95Z-ZRsmDeq0sKpE3Ka844x5qxWz8mJkFoJZvmGXN_cIcWuw2amsaM4lCXFQ_UyNfOSkMaJxjxjnOP4QG9hT-8Qhn66pf1EgSaYadfXywB0jC0OL8izDoaML4_zjHz_9PFm-7m6vL74sn1_WdXKmrnSqBshRMO0ljVo1dYOlLTSgHS-RdcZh963XHjObd2BcYojByfBNNqAlmfk7dq7T_F-wTyHsc8NDgNMGJccuNRWeW-YL-ibf9BdXNJUviuU91pJ7k2h5Eo1KeacsAv71I-QHgJn4eA7HH2Hg--w-i6p18fupT7cHjOPggtQrUBfHP7-e4f0KxgrrQ5XP7bh209mtPjwNVwV_nzl63H3Xx_8AUMBmYM</recordid><startdate>201306</startdate><enddate>201306</enddate><creator>Inoue, Motohiro</creator><creator>Nakajima, Miwa</creator><creator>Hojo, Tatsuya</creator><creator>Itoi, Megumi</creator><creator>Kitakoji, Hiroshi</creator><general>BMJ Publishing Group Ltd</general><general>Sage Publications Ltd</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7RV</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AN0</scope><scope>BENPR</scope><scope>BTHHO</scope><scope>CCPQU</scope><scope>EHMNL</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>KB0</scope><scope>M0S</scope><scope>M1P</scope><scope>NAPCQ</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope></search><sort><creationdate>201306</creationdate><title>The effect of electroacupuncture on osteotomy gap healing in a rat fibula model</title><author>Inoue, Motohiro ; Nakajima, Miwa ; Hojo, Tatsuya ; Itoi, Megumi ; Kitakoji, Hiroshi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-b476t-5e5c222c0553ba54db8a43736a389de8f68e99d129117bfa6841e1a83a6c56a53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Animals</topic><topic>Disease Models, Animal</topic><topic>Electroacupuncture - methods</topic><topic>Fibula - injuries</topic><topic>Fibula - pathology</topic><topic>Fracture Healing - physiology</topic><topic>Fractures, Bone - therapy</topic><topic>Osteotomy</topic><topic>Rats</topic><topic>Rats, Wistar</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Inoue, Motohiro</creatorcontrib><creatorcontrib>Nakajima, Miwa</creatorcontrib><creatorcontrib>Hojo, Tatsuya</creatorcontrib><creatorcontrib>Itoi, Megumi</creatorcontrib><creatorcontrib>Kitakoji, Hiroshi</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Nursing & Allied Health Database</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>British Nursing Database</collection><collection>ProQuest Central</collection><collection>BMJ Journals</collection><collection>ProQuest One Community College</collection><collection>UK & Ireland Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Nursing & Allied Health Premium</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><jtitle>Acupuncture in medicine : journal of the British Medical Acupuncture Society</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Inoue, Motohiro</au><au>Nakajima, Miwa</au><au>Hojo, Tatsuya</au><au>Itoi, Megumi</au><au>Kitakoji, Hiroshi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The effect of electroacupuncture on osteotomy gap healing in a rat fibula model</atitle><jtitle>Acupuncture in medicine : journal of the British Medical Acupuncture Society</jtitle><addtitle>Acupunct Med</addtitle><date>2013-06</date><risdate>2013</risdate><volume>31</volume><issue>2</issue><spage>222</spage><epage>227</epage><pages>222-227</pages><issn>0964-5284</issn><eissn>1759-9873</eissn><abstract>Objective To examine the effect of electroacupuncture (EA) on osteotomy gap healing in a rat fibula model. Methods A total of 40 12-week-old male Wistar rats underwent unilateral open osteotomy of the fibula to create a 2 mm gap. The rats were randomly assigned to an EA group (n=20) and a control group (n=20). In the EA group, a cathodal electrode was connected to an acupuncture needle percutaneously at the surgery site, while another acupuncture needle inserted 15 mm proximal to the surgery site was used as an anodal electrode. EA was performed 5 days a week for 6 weeks. The control group received no treatment. Some rats were killed at 3 days and 1 week after surgery and the cut end of the distal part of the fibula was surgically removed and histologically assessed. Haematoxylin and eosin (H&E) staining was used to measure total cell count and immunohistochemical staining to assess the increase in the bone morphogenetic protein 2 (BMP-2)-positive cells. The healing process was also assessed weekly after surgery via x-ray examinations. Results At each time point, total cell count showed a marked increase in the EA group (p<0.05), while BMP-2 expression showed a tendency to increase in the EA group. Radiological examination showed a marked reduction in the distance between the cut ends of the fibula in the EA group. Conclusions There was a marked increase in cell count and expression of growth factor in the EA group. These results indicate direct current EA could be useful for promoting bone healing.</abstract><cop>England</cop><pub>BMJ Publishing Group Ltd</pub><pmid>23542071</pmid><doi>10.1136/acupmed-2012-010294</doi><tpages>6</tpages></addata></record> |
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| subjects | Animals Disease Models, Animal Electroacupuncture - methods Fibula - injuries Fibula - pathology Fracture Healing - physiology Fractures, Bone - therapy Osteotomy Rats Rats, Wistar |
| title | The effect of electroacupuncture on osteotomy gap healing in a rat fibula model |
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