Mouse strain and quality control testing: improved sensitivity of the mouse embryo assay with embryos from outbred mice
Objective To determine the relative sensitivities of embryos from different strains of mice to in vitro stress. Design Laboratory experiment with embryos from different mouse strains. Setting University hospital–based fertility clinic. Animal(s) Mice. Intervention(s) Fresh one-cell embryos from outb...
Gespeichert in:
| Veröffentlicht in: | Fertility and sterility 2013-03, Vol.99 (3), p.847-854.e2 |
|---|---|
| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 854.e2 |
|---|---|
| container_issue | 3 |
| container_start_page | 847 |
| container_title | Fertility and sterility |
| container_volume | 99 |
| creator | Khan, Zaraq, M.B.B.S Wolff, Heather S., M.D Fredrickson, Jolene R., M.S Walker, David L., M.S Daftary, Gaurang S., M.D Morbeck, Dean E., Ph.D |
| description | Objective To determine the relative sensitivities of embryos from different strains of mice to in vitro stress. Design Laboratory experiment with embryos from different mouse strains. Setting University hospital–based fertility clinic. Animal(s) Mice. Intervention(s) Fresh one-cell embryos from outbred (CF1), inbred (FVB), F1 hybrid (B6/CBA), and cryopreserved F2 hybrid embryos (bcl/B6 × B6/bcl) compared in a mouse embryo assay (MEA) using six doses of each of three in vitro stressors: cumene hydroperoxide in mineral oil, Triton X-100 (TX-100) in media, and hyperosmolality. Main Outcome Measure(s) Blastocyst rate at 96 hours. Result(s) All studies were conducted in triplicate; data were analyzed with chi-square analysis based on fitting a logistic regression model. Both cumene hydroperoxide and Triton X-100 affected blastocyst formation in the outbred strain at concentrations that were less than half of the concentration that affected the other strains. The total number of cells was affected by the treatments in all strains. Conclusion(s) Outbred CF1 embryos are genetically diverse and more sensitive to toxins than either inbred or hybrid mouse embryos. Outbred embryos provide an additional tool for effective quality-control testing. |
| doi_str_mv | 10.1016/j.fertnstert.2012.10.046 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1356954947</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0015028212023904</els_id><sourcerecordid>1356954947</sourcerecordid><originalsourceid>FETCH-LOGICAL-c503t-624f771b5336e329e09e0ecaf75d7396af65aae81c2b3df677b7a7784c8cf0833</originalsourceid><addsrcrecordid>eNqNkk1v1DAQhi0EokvhL4CPXLL4I7YTDki0ghapiEPp2XKccesliVvb2Sr_HoddQOKEZNnS-Jl3xq8HIUzJlhIq3-22DmKeUi77lhHKSnhLavkEbagQshJS8KdoQwgVFWENO0EvUtoRQiRV7Dk6YZy2rSTtBj1-DXMCnHI0fsJm6vHDbAafF2zDlGMYcIaU_XT7HvvxPoY99DjBlHz2-5UKDuc7wOMvFRi7uARsUjILfvT57hhJ2MUw4jDnLpb80Vt4iZ45MyR4dTxP0c3nT9_PL6urbxdfzj9eVVYQnivJaqcU7QTnEjhrgZQF1jglesVbaZwUxkBDLet476RSnTJKNbVtrCMN56fo7UG39P4wl6fo0ScLw2AmKD1ryoVsRd3WqqDNAbUxpBTB6fvoRxMXTYlebdc7_dd2vdq-3hTbS-rrY5W5G6H_k_jb5wK8OQDOBG1uo0_65rooiPJFrGkYK8TZgYDixt5D1Ml6mCz0PoLNug_-f_r48I-IHfzkrRl-wAJpF-Y4Fbc11Ylpoq_XAVnngzLCeEtq_hP_Jrno</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1356954947</pqid></control><display><type>article</type><title>Mouse strain and quality control testing: improved sensitivity of the mouse embryo assay with embryos from outbred mice</title><source>MEDLINE</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>ScienceDirect Journals (5 years ago - present)</source><source>Alma/SFX Local Collection</source><creator>Khan, Zaraq, M.B.B.S ; Wolff, Heather S., M.D ; Fredrickson, Jolene R., M.S ; Walker, David L., M.S ; Daftary, Gaurang S., M.D ; Morbeck, Dean E., Ph.D</creator><creatorcontrib>Khan, Zaraq, M.B.B.S ; Wolff, Heather S., M.D ; Fredrickson, Jolene R., M.S ; Walker, David L., M.S ; Daftary, Gaurang S., M.D ; Morbeck, Dean E., Ph.D</creatorcontrib><description>Objective To determine the relative sensitivities of embryos from different strains of mice to in vitro stress. Design Laboratory experiment with embryos from different mouse strains. Setting University hospital–based fertility clinic. Animal(s) Mice. Intervention(s) Fresh one-cell embryos from outbred (CF1), inbred (FVB), F1 hybrid (B6/CBA), and cryopreserved F2 hybrid embryos (bcl/B6 × B6/bcl) compared in a mouse embryo assay (MEA) using six doses of each of three in vitro stressors: cumene hydroperoxide in mineral oil, Triton X-100 (TX-100) in media, and hyperosmolality. Main Outcome Measure(s) Blastocyst rate at 96 hours. Result(s) All studies were conducted in triplicate; data were analyzed with chi-square analysis based on fitting a logistic regression model. Both cumene hydroperoxide and Triton X-100 affected blastocyst formation in the outbred strain at concentrations that were less than half of the concentration that affected the other strains. The total number of cells was affected by the treatments in all strains. Conclusion(s) Outbred CF1 embryos are genetically diverse and more sensitive to toxins than either inbred or hybrid mouse embryos. Outbred embryos provide an additional tool for effective quality-control testing.</description><identifier>ISSN: 0015-0282</identifier><identifier>EISSN: 1556-5653</identifier><identifier>DOI: 10.1016/j.fertnstert.2012.10.046</identifier><identifier>PMID: 23199609</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Animals ; Animals, Outbred Strains ; Benzene Derivatives - toxicity ; Biological Assay - methods ; blastocyst ; Chimera ; cryopreservation ; Detergents - toxicity ; Embryo culture ; Embryo, Mammalian - embryology ; Female ; hybrids ; Internal Medicine ; in vitro fertilization ; laboratory experimentation ; Male ; Mice ; mineral oil ; mouse strain ; Obstetrics and Gynecology ; octoxynol ; Octoxynol - toxicity ; Osmolar Concentration ; Osmotic Pressure - physiology ; outbred mouse embryos ; Oxidants - toxicity ; Oxidative Stress - drug effects ; Oxidative Stress - physiology ; Pregnancy ; Quality Control ; regression analysis ; Reproductive Medicine - methods ; Reproductive Medicine - standards ; Species Specificity ; toxicity ; toxins</subject><ispartof>Fertility and sterility, 2013-03, Vol.99 (3), p.847-854.e2</ispartof><rights>American Society for Reproductive Medicine</rights><rights>2013 American Society for Reproductive Medicine</rights><rights>Copyright © 2013 American Society for Reproductive Medicine. Published by Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c503t-624f771b5336e329e09e0ecaf75d7396af65aae81c2b3df677b7a7784c8cf0833</citedby><cites>FETCH-LOGICAL-c503t-624f771b5336e329e09e0ecaf75d7396af65aae81c2b3df677b7a7784c8cf0833</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.fertnstert.2012.10.046$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,777,781,3537,27905,27906,45976</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23199609$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Khan, Zaraq, M.B.B.S</creatorcontrib><creatorcontrib>Wolff, Heather S., M.D</creatorcontrib><creatorcontrib>Fredrickson, Jolene R., M.S</creatorcontrib><creatorcontrib>Walker, David L., M.S</creatorcontrib><creatorcontrib>Daftary, Gaurang S., M.D</creatorcontrib><creatorcontrib>Morbeck, Dean E., Ph.D</creatorcontrib><title>Mouse strain and quality control testing: improved sensitivity of the mouse embryo assay with embryos from outbred mice</title><title>Fertility and sterility</title><addtitle>Fertil Steril</addtitle><description>Objective To determine the relative sensitivities of embryos from different strains of mice to in vitro stress. Design Laboratory experiment with embryos from different mouse strains. Setting University hospital–based fertility clinic. Animal(s) Mice. Intervention(s) Fresh one-cell embryos from outbred (CF1), inbred (FVB), F1 hybrid (B6/CBA), and cryopreserved F2 hybrid embryos (bcl/B6 × B6/bcl) compared in a mouse embryo assay (MEA) using six doses of each of three in vitro stressors: cumene hydroperoxide in mineral oil, Triton X-100 (TX-100) in media, and hyperosmolality. Main Outcome Measure(s) Blastocyst rate at 96 hours. Result(s) All studies were conducted in triplicate; data were analyzed with chi-square analysis based on fitting a logistic regression model. Both cumene hydroperoxide and Triton X-100 affected blastocyst formation in the outbred strain at concentrations that were less than half of the concentration that affected the other strains. The total number of cells was affected by the treatments in all strains. Conclusion(s) Outbred CF1 embryos are genetically diverse and more sensitive to toxins than either inbred or hybrid mouse embryos. Outbred embryos provide an additional tool for effective quality-control testing.</description><subject>Animals</subject><subject>Animals, Outbred Strains</subject><subject>Benzene Derivatives - toxicity</subject><subject>Biological Assay - methods</subject><subject>blastocyst</subject><subject>Chimera</subject><subject>cryopreservation</subject><subject>Detergents - toxicity</subject><subject>Embryo culture</subject><subject>Embryo, Mammalian - embryology</subject><subject>Female</subject><subject>hybrids</subject><subject>Internal Medicine</subject><subject>in vitro fertilization</subject><subject>laboratory experimentation</subject><subject>Male</subject><subject>Mice</subject><subject>mineral oil</subject><subject>mouse strain</subject><subject>Obstetrics and Gynecology</subject><subject>octoxynol</subject><subject>Octoxynol - toxicity</subject><subject>Osmolar Concentration</subject><subject>Osmotic Pressure - physiology</subject><subject>outbred mouse embryos</subject><subject>Oxidants - toxicity</subject><subject>Oxidative Stress - drug effects</subject><subject>Oxidative Stress - physiology</subject><subject>Pregnancy</subject><subject>Quality Control</subject><subject>regression analysis</subject><subject>Reproductive Medicine - methods</subject><subject>Reproductive Medicine - standards</subject><subject>Species Specificity</subject><subject>toxicity</subject><subject>toxins</subject><issn>0015-0282</issn><issn>1556-5653</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkk1v1DAQhi0EokvhL4CPXLL4I7YTDki0ghapiEPp2XKccesliVvb2Sr_HoddQOKEZNnS-Jl3xq8HIUzJlhIq3-22DmKeUi77lhHKSnhLavkEbagQshJS8KdoQwgVFWENO0EvUtoRQiRV7Dk6YZy2rSTtBj1-DXMCnHI0fsJm6vHDbAafF2zDlGMYcIaU_XT7HvvxPoY99DjBlHz2-5UKDuc7wOMvFRi7uARsUjILfvT57hhJ2MUw4jDnLpb80Vt4iZ45MyR4dTxP0c3nT9_PL6urbxdfzj9eVVYQnivJaqcU7QTnEjhrgZQF1jglesVbaZwUxkBDLet476RSnTJKNbVtrCMN56fo7UG39P4wl6fo0ScLw2AmKD1ryoVsRd3WqqDNAbUxpBTB6fvoRxMXTYlebdc7_dd2vdq-3hTbS-rrY5W5G6H_k_jb5wK8OQDOBG1uo0_65rooiPJFrGkYK8TZgYDixt5D1Ml6mCz0PoLNug_-f_r48I-IHfzkrRl-wAJpF-Y4Fbc11Ylpoq_XAVnngzLCeEtq_hP_Jrno</recordid><startdate>20130301</startdate><enddate>20130301</enddate><creator>Khan, Zaraq, M.B.B.S</creator><creator>Wolff, Heather S., M.D</creator><creator>Fredrickson, Jolene R., M.S</creator><creator>Walker, David L., M.S</creator><creator>Daftary, Gaurang S., M.D</creator><creator>Morbeck, Dean E., Ph.D</creator><general>Elsevier Inc</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20130301</creationdate><title>Mouse strain and quality control testing: improved sensitivity of the mouse embryo assay with embryos from outbred mice</title><author>Khan, Zaraq, M.B.B.S ; Wolff, Heather S., M.D ; Fredrickson, Jolene R., M.S ; Walker, David L., M.S ; Daftary, Gaurang S., M.D ; Morbeck, Dean E., Ph.D</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c503t-624f771b5336e329e09e0ecaf75d7396af65aae81c2b3df677b7a7784c8cf0833</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Animals</topic><topic>Animals, Outbred Strains</topic><topic>Benzene Derivatives - toxicity</topic><topic>Biological Assay - methods</topic><topic>blastocyst</topic><topic>Chimera</topic><topic>cryopreservation</topic><topic>Detergents - toxicity</topic><topic>Embryo culture</topic><topic>Embryo, Mammalian - embryology</topic><topic>Female</topic><topic>hybrids</topic><topic>Internal Medicine</topic><topic>in vitro fertilization</topic><topic>laboratory experimentation</topic><topic>Male</topic><topic>Mice</topic><topic>mineral oil</topic><topic>mouse strain</topic><topic>Obstetrics and Gynecology</topic><topic>octoxynol</topic><topic>Octoxynol - toxicity</topic><topic>Osmolar Concentration</topic><topic>Osmotic Pressure - physiology</topic><topic>outbred mouse embryos</topic><topic>Oxidants - toxicity</topic><topic>Oxidative Stress - drug effects</topic><topic>Oxidative Stress - physiology</topic><topic>Pregnancy</topic><topic>Quality Control</topic><topic>regression analysis</topic><topic>Reproductive Medicine - methods</topic><topic>Reproductive Medicine - standards</topic><topic>Species Specificity</topic><topic>toxicity</topic><topic>toxins</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Khan, Zaraq, M.B.B.S</creatorcontrib><creatorcontrib>Wolff, Heather S., M.D</creatorcontrib><creatorcontrib>Fredrickson, Jolene R., M.S</creatorcontrib><creatorcontrib>Walker, David L., M.S</creatorcontrib><creatorcontrib>Daftary, Gaurang S., M.D</creatorcontrib><creatorcontrib>Morbeck, Dean E., Ph.D</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Fertility and sterility</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Khan, Zaraq, M.B.B.S</au><au>Wolff, Heather S., M.D</au><au>Fredrickson, Jolene R., M.S</au><au>Walker, David L., M.S</au><au>Daftary, Gaurang S., M.D</au><au>Morbeck, Dean E., Ph.D</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Mouse strain and quality control testing: improved sensitivity of the mouse embryo assay with embryos from outbred mice</atitle><jtitle>Fertility and sterility</jtitle><addtitle>Fertil Steril</addtitle><date>2013-03-01</date><risdate>2013</risdate><volume>99</volume><issue>3</issue><spage>847</spage><epage>854.e2</epage><pages>847-854.e2</pages><issn>0015-0282</issn><eissn>1556-5653</eissn><abstract>Objective To determine the relative sensitivities of embryos from different strains of mice to in vitro stress. Design Laboratory experiment with embryos from different mouse strains. Setting University hospital–based fertility clinic. Animal(s) Mice. Intervention(s) Fresh one-cell embryos from outbred (CF1), inbred (FVB), F1 hybrid (B6/CBA), and cryopreserved F2 hybrid embryos (bcl/B6 × B6/bcl) compared in a mouse embryo assay (MEA) using six doses of each of three in vitro stressors: cumene hydroperoxide in mineral oil, Triton X-100 (TX-100) in media, and hyperosmolality. Main Outcome Measure(s) Blastocyst rate at 96 hours. Result(s) All studies were conducted in triplicate; data were analyzed with chi-square analysis based on fitting a logistic regression model. Both cumene hydroperoxide and Triton X-100 affected blastocyst formation in the outbred strain at concentrations that were less than half of the concentration that affected the other strains. The total number of cells was affected by the treatments in all strains. Conclusion(s) Outbred CF1 embryos are genetically diverse and more sensitive to toxins than either inbred or hybrid mouse embryos. Outbred embryos provide an additional tool for effective quality-control testing.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>23199609</pmid><doi>10.1016/j.fertnstert.2012.10.046</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0015-0282 |
| ispartof | Fertility and sterility, 2013-03, Vol.99 (3), p.847-854.e2 |
| issn | 0015-0282 1556-5653 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1356954947 |
| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; ScienceDirect Journals (5 years ago - present); Alma/SFX Local Collection |
| subjects | Animals Animals, Outbred Strains Benzene Derivatives - toxicity Biological Assay - methods blastocyst Chimera cryopreservation Detergents - toxicity Embryo culture Embryo, Mammalian - embryology Female hybrids Internal Medicine in vitro fertilization laboratory experimentation Male Mice mineral oil mouse strain Obstetrics and Gynecology octoxynol Octoxynol - toxicity Osmolar Concentration Osmotic Pressure - physiology outbred mouse embryos Oxidants - toxicity Oxidative Stress - drug effects Oxidative Stress - physiology Pregnancy Quality Control regression analysis Reproductive Medicine - methods Reproductive Medicine - standards Species Specificity toxicity toxins |
| title | Mouse strain and quality control testing: improved sensitivity of the mouse embryo assay with embryos from outbred mice |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-19T17%3A55%3A32IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Mouse%20strain%20and%20quality%20control%20testing:%20improved%20sensitivity%20of%20the%20mouse%20embryo%20assay%20with%20embryos%20from%20outbred%20mice&rft.jtitle=Fertility%20and%20sterility&rft.au=Khan,%20Zaraq,%20M.B.B.S&rft.date=2013-03-01&rft.volume=99&rft.issue=3&rft.spage=847&rft.epage=854.e2&rft.pages=847-854.e2&rft.issn=0015-0282&rft.eissn=1556-5653&rft_id=info:doi/10.1016/j.fertnstert.2012.10.046&rft_dat=%3Cproquest_cross%3E1356954947%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1356954947&rft_id=info:pmid/23199609&rft_els_id=S0015028212023904&rfr_iscdi=true |